Status
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Gene information
General information
TC008163 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5928378..5959702
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008163
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04438

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (22 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    elytra shortened - Number of animals showing the phenotype: 8

    legs shortened - Number of animals showing the phenotype: 8

    abdomen gin-traps slightlysize decreased - Number of animals showing the phenotype: 8

    Comment: Gin traps pointing to dorsal instead of lateral. Whole gin trap closely attached to the trunk.
    abdomen gin-traps orientation irregular - Number of animals showing the phenotype: 8

    female genital lobes shortened - Number of animals showing the phenotype: 8

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 8


    elytra surface irregular - Number of animals showing the phenotype: 8


    legs femur shortened - Number of animals showing the phenotype: 8


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008163 RA
      MVVITQFYH ESARGYPKI RPQEFQRDL TSNNITALN 
      ETSLSHYTH MEELTLSEN KLESIHPKS FAKNVQLKR 
      LLLQGCALS EIPVEVLRP LSKLQTLHL GNNEIWKLD 
      GTTFQQVPA LRSLRLDGN RLRGVPSEA LSSLLHLEV 
      LNIGNNLIN ALPPAAFPS LDKLVVLLM KRNQISEIA 
      EEAFANLTS LKVLELDDN FLTEIPAAV TKLAKLQEL 
      SISGNRIKY IRGGLLQKT PALALLELK GNPLTGVDA 
      HAFSFLPRL RKLILSEAR ELSTFPNLN GTTALEILR 
      LDRAGISSV PSTLCTTCP RLKSLDLKS NKLKTIPDL 
      NDCREMRVL DLASNHIRT LENRPFRGM YQMHDLLLA 
      HNEIQYIPQ DAFYNLSRL QVLNLEDNQ ISFIHPDAF 
      LPISKIEDL NLGQNVFPH LPSAGLERL LHLKTFNNP 
      NLREFPPPE EFPRIQTLV LAYAYHCCA FLPLIPSNP 
      PPKAKDFIV FPDIEDIDM NMWNSSLND YWPSQQNLS 
      HKFGKKFET IWENLRSDF TYPGNFPSY MEEYAEEEA 
      QRVAGGDGP PGKIQCLPL PGPFLPCQD LFDWWTLRC 
      GVWIVFLCA MLGNGTVVF VLIFSRGKM DVPRFLVCN 
      LAAADFFMG IYLGFLAVV DASTLGEFR MYAIPWQMS 
      AGCQLSGFL GVLSSELSV YTLAVITLE RNYAITHAM 
      HLNKRLSLK HAGYIMICG WSFAIVMGL LPLFSVSDY 
      RKFAVCLPF ETKDAASLT YVVFLMFIN GVAFLILMG 
      CYLKMYCAI RGSQAWNSN DSRIAKRMA LLVFTDFLC 
      WSPIAFFSL TAAFGLQLI SLEQAKVFT VFVLPLNSC 
      CNPFLYAIL TKQFKKDCV MICKAIEES RVTRGIGRC 
      RHSSNFSNR QTPANTNSL ADRSSRENQ NHVPTCTCN 
      VKLLGERSA PPPRTDRKT RTREWLLSK ARWLLCVRR 
      QPRHRPRSD QYTYQIAEI QQKQHKRAS SVSSSENFS 
      SSRSDSWRH NHHCGIPLR LLDPKRRAS SWLVTRKTS 
      QDSNLSSSR NDSSGSATT ASTSTWRMS RSSASSEPN 
      RIRAKPRLT RQSAIQDEA DLPGSPGRL TVRFLTTIP 
      SAAETSMQM EDEQPAILV SPSKEREGP LYAILHSQP 
      VTPARRQSI VTLHPPETI HNSSQPSEA GPSR
    CDS sequence
    • >TC008163 RA
      atggttgta attacgcaa ttttaccat gaatcagcc 
      cgcgggtac ccgaaaatc cgaccgcag gaattccaa 
      cgagacctg acaagcaac aacatcacg gccctcaac 
      gaaacctcg ctgtcccac tacacccac atggaggaa 
      ctgacgctc tccgagaac aaactcgag tccatccac 
      ccgaaatcg ttcgcaaag aacgtccag ttaaagaga 
      ttacttttg caagggtgc gccctctcc gagatcccc 
      gtggaggtc ctccgcccc ctcagcaaa ctccaaact 
      ttgcatctg ggcaataac gaaatctgg aaactggat 
      ggaaccact ttccagcag gttcctgca ctgcgcagc 
      ttgcgtctg gacggcaac cgtctgcgc ggtgttcct 
      tcggaggca ctgtccagt ctcctgcac cttgaggtc 
      ctgaacatt ggaaataat ctcatcaac gccctccct 
      ccggccgct ttcccatcg ctggataaa ctcgtcgta 
      ttattgatg aaacgtaac cagataagt gagatcgca 
      gaggaagct ttcgctaac ttgacgtcg ctgaaagtc 
      ctcgagctg gacgacaat ttcctgact gagatacct 
      gcagcagtc acgaagctc gccaaactg caggaacta 
      tcgatatct ggtaaccgc atcaagtat atccgtggg 
      ggcttactg cagaagaca cccgcgctg gctctgtta 
      gagctgaaa gggaatcct ttgacggga gtggacgcc 
      cacgctttc tcctttctg ccgcgactt cgaaagctg 
      attctgtct gaggcaaga gagttgtcg acgttcccc 
      aacttgaac ggcacaacg gccttggag atcctaagg 
      ttggatagg gcagggatt tcttccgtc ccttccact 
      ctctgcacg acttgtccc agactcaaa agcttggac 
      ctcaagtcg aacaagctt aaaactata cctgacctc 
      aacgactgc agggagatg agagtcctg gatttggcc 
      agcaaccac atccggacg ctggaaaac aggccattt 
      cggggcatg taccaaatg cacgaccta ctcctggct 
      cacaacgaa atccaatac atcccgcaa gacgccttc 
      tacaacctc tccaggctc caagtctta aatctcgaa 
      gacaaccag atttctttc atacacccg gacgccttc 
      cttccgatt tcaaaaatc gaggattta aatctgggc 
      cagaatgtt tttccgcat ctaccgtcg gccggttta 
      gagcgcctc ctccattta aaaactttc aataacccg 
      aacttgcgt gaattccca ccgccggag gaattcccc 
      cgtatccag actttggtc ctggcgtac gcttaccac 
      tgctgcgct tttttacct ctgataccg tcaaacccg 
      cccccgaaa gccaaagat ttcatcgtg tttccagac 
      attgaagat attgacatg aacatgtgg aactccagt 
      ttgaacgac tactggcct tcgcaacaa aatttgagt 
      cacaagttt ggtaaaaaa ttcgaaact atttgggaa 
      aacctccgg tcggatttc acctacccg gggaatttt 
      ccctcctac atggaggaa tacgccgaa gaggaggcc 
      caacgagtg gcgggcggc gacggcccg cctggaaaa 
      atccagtgt ttgcctttg ccaggcccg tttttaccg 
      tgccaggac ctgttcgac tggtggacg ctgcggtgt 
      ggcgtctgg atcgtgttt ctctgtgcc atgttgggc 
      aatgggacc gtggtgttt gtgctgatt ttttccagg 
      gggaaaatg gacgttccc cggtttctg gtgtgcaac 
      ttggccgcg gccgatttc ttcatgggc atttattta 
      ggtttcttg gccgtggtg gacgcctcc acccttggc 
      gagttccgg atgtacgcc attccgtgg cagatgtcc 
      gccggttgt caattatcc ggcttcttg ggtgtgctc 
      agttcggaa ctgtcagtc tacactctg gccgtaatc 
      actctcgaa cgcaattac gccatcact cacgccatg 
      catttgaac aaaaggctt tcgctgaaa cacgccggt 
      tatataatg atctgcggc tggagcttt gccatcgtc 
      atggggctc ctccccctg ttcagcgtc tccgactac 
      cgcaagttc gccgtttgt ctcccgttt gaaaccaaa 
      gatgcggcg agcctgacc tacgtcgtc ttcctcatg 
      ttcatcaac ggagtggcg tttttgatc ctaatgggc 
      tgctatctc aagatgtat tgcgccatt cggggctcc 
      caagcctgg aactcgaac gattcccga atcgccaaa 
      cgcatggcc ttgctagtg ttcaccgac tttttgtgt 
      tggtcccca atcgcgttc ttctccctc acggcagcg 
      ttcggcttg cagttaatc tccctcgaa caagctaaa 
      gtgttcacc gtgtttgtc ctaccgctg aattcgtgc 
      tgcaacccg ttcttgtac gcgattctg actaaacag 
      ttcaaaaag gattgtgtg atgatttgt aaagcgatt 
      gaagagagc cgagtgacc cgcggaatc ggccgttgt 
      agacacagt tctaacttt agcaataga caaactcca 
      gcgaatact aacagtttg gcggaccgc tcctcccgt 
      gaaaaccaa aaccacgtg cccacgtgt acttgtaac 
      gtaaaactc ctgggcgag agaagcgcc cctccgcca 
      cgaactgac cgaaagacc cgaacccgg gagtggctc 
      ttatccaaa gctcgctgg ttgctgtgc gtgaggaga 
      cagcccaga caccggcca cgctccgac caatacacc 
      tatcagatc gctgaaatc cagcagaag cagcacaag 
      agggcctcg tccgtctcc tccagcgag aacttcagc 
      tcgagtcgc tccgactcc tggcgccac aaccaccac 
      tgcggcatc ccgctccgg ctgctggac cccaaacgg 
      cgcgcgtct tcgtggcta gtgacgcgc aagacgtcc 
      caagattcc aacttgtcg agctcgagg aacgactcc 
      tcgggctcg gccaccaca gccagcacc agcacgtgg 
      cgcatgtcg cggtcgagc gcgtccagc gagccgaac 
      cgaatcagg gccaagccc agactgacg agacagagt 
      gccatccag gacgaggcg gacctgccg gggtccccc 
      gggcggctc acggtgcgc ttcctgacg accattccg 
      tcggcggct gagaccagt atgcagatg gaggacgaa 
      cagccggcg attttggtg agtccgtcc aaggagcgc 
      gaaggcccc ctctatgcc attttgcac tcgcagccg 
      gtcacgccc gccaggagg cagagcatt gtgacgctg 
      catccgccg gagacgatc cataattct agtcagccc 
      agtgaagca gggccttcc agataa
    mRNA sequence
    • >TC008163 RA
      gtgaactgg tatggttgt aattacgca attttacca 
      tgaatcagc ccgcgggta cccgaaaat ccgaccgca 
      ggaattcca acgagacct gacaagcaa caacatcac 
      ggccctcaa cgaaacctc gctgtccca ctacaccca 
      catggagga actgacgct ctccgagaa caaactcga 
      gtccatcca cccgaaatc gttcgcaaa gaacgtcca 
      gttaaagag attactttt gcaagggtg cgccctctc 
      cgagatccc cgtggaggt cctccgccc cctcagcaa 
      actccaaac tttgcatct gggcaataa cgaaatctg 
      gaaactgga tggaaccac tttccagca ggttcctgc 
      actgcgcag cttgcgtct ggacggcaa ccgtctgcg 
      cggtgttcc ttcggaggc actgtccag tctcctgca 
      ccttgaggt cctgaacat tggaaataa tctcatcaa 
      cgccctccc tccggccgc tttcccatc gctggataa 
      actcgtcgt attattgat gaaacgtaa ccagataag 
      tgagatcgc agaggaagc tttcgctaa cttgacgtc 
      gctgaaagt cctcgagct ggacgacaa tttcctgac 
      tgagatacc tgcagcagt cacgaagct cgccaaact 
      gcaggaact atcgatatc tggtaaccg catcaagta 
      tatccgtgg gggcttact gcagaagac acccgcgct 
      ggctctgtt agagctgaa agggaatcc tttgacggg 
      agtggacgc ccacgcttt ctcctttct gccgcgact 
      tcgaaagct gattctgtc tgaggcaag agagttgtc 
      gacgttccc caacttgaa cggcacaac ggccttgga 
      gatcctaag gttggatag ggcagggat ttcttccgt 
      cccttccac tctctgcac gacttgtcc cagactcaa 
      aagcttgga cctcaagtc gaacaagct taaaactat 
      acctgacct caacgactg cagggagat gagagtcct 
      ggatttggc cagcaacca catccggac gctggaaaa 
      caggccatt tcggggcat gtaccaaat gcacgacct 
      actcctggc tcacaacga aatccaata catcccgca 
      agacgcctt ctacaacct ctccaggct ccaagtctt 
      aaatctcga agacaacca gatttcttt catacaccc 
      ggacgcctt ccttccgat ttcaaaaat cgaggattt 
      aaatctggg ccagaatgt ttttccgca tctaccgtc 
      ggccggttt agagcgcct cctccattt aaaaacttt 
      caataaccc gaacttgcg tgaattccc accgccgga 
      ggaattccc ccgtatcca gactttggt cctggcgta 
      cgcttacca ctgctgcgc ttttttacc tctgatacc 
      gtcaaaccc gcccccgaa agccaaaga tttcatcgt 
      gtttccaga cattgaaga tattgacat gaacatgtg 
      gaactccag tttgaacga ctactggcc ttcgcaaca 
      aaatttgag tcacaagtt tggtaaaaa attcgaaac 
      tatttggga aaacctccg gtcggattt cacctaccc 
      ggggaattt tccctccta catggagga atacgccga 
      agaggaggc ccaacgagt ggcgggcgg cgacggccc 
      gcctggaaa aatccagtg tttgccttt gccaggccc 
      gtttttacc gtgccagga cctgttcga ctggtggac 
      gctgcggtg tggcgtctg gatcgtgtt tctctgtgc 
      catgttggg caatgggac cgtggtgtt tgtgctgat 
      tttttccag ggggaaaat ggacgttcc ccggtttct 
      ggtgtgcaa cttggccgc ggccgattt cttcatggg 
      catttattt aggtttctt ggccgtggt ggacgcctc 
      cacccttgg cgagttccg gatgtacgc cattccgtg 
      gcagatgtc cgccggttg tcaattatc cggcttctt 
      gggtgtgct cagttcgga actgtcagt ctacactct 
      ggccgtaat cactctcga acgcaatta cgccatcac 
      tcacgccat gcatttgaa caaaaggct ttcgctgaa 
      acacgccgg ttatataat gatctgcgg ctggagctt 
      tgccatcgt catggggct cctccccct gttcagcgt 
      ctccgacta ccgcaagtt cgccgtttg tctcccgtt 
      tgaaaccaa agatgcggc gagcctgac ctacgtcgt 
      cttcctcat gttcatcaa cggagtggc gtttttgat 
      cctaatggg ctgctatct caagatgta ttgcgccat 
      tcggggctc ccaagcctg gaactcgaa cgattcccg 
      aatcgccaa acgcatggc cttgctagt gttcaccga 
      ctttttgtg ttggtcccc aatcgcgtt cttctccct 
      cacggcagc gttcggctt gcagttaat ctccctcga 
      acaagctaa agtgttcac cgtgtttgt cctaccgct 
      gaattcgtg ctgcaaccc gttcttgta cgcgattct 
      gactaaaca gttcaaaaa ggattgtgt gatgatttg 
      taaagcgat tgaagagag ccgagtgac ccgcggaat 
      cggccgttg tagacacag ttctaactt tagcaatag 
      acaaactcc agcgaatac taacagttt ggcggaccg 
      ctcctcccg tgaaaacca aaaccacgt gcccacgtg 
      tacttgtaa cgtaaaact cctgggcga gagaagcgc 
      ccctccgcc acgaactga ccgaaagac ccgaacccg 
      ggagtggct cttatccaa agctcgctg gttgctgtg 
      cgtgaggag acagcccag acaccggcc acgctccga 
      ccaatacac ctatcagat cgctgaaat ccagcagaa 
      gcagcacaa gagggcctc gtccgtctc ctccagcga 
      gaacttcag ctcgagtcg ctccgactc ctggcgcca 
      caaccacca ctgcggcat cccgctccg gctgctgga 
      ccccaaacg gcgcgcgtc ttcgtggct agtgacgcg 
      caagacgtc ccaagattc caacttgtc gagctcgag 
      gaacgactc ctcgggctc ggccaccac agccagcac 
      cagcacgtg gcgcatgtc gcggtcgag cgcgtccag 
      cgagccgaa ccgaatcag ggccaagcc cagactgac 
      gagacagag tgccatcca ggacgaggc ggacctgcc 
      ggggtcccc cgggcggct cacggtgcg cttcctgac 
      gaccattcc gtcggcggc tgagaccag tatgcagat 
      ggaggacga acagccggc gattttggt gagtccgtc 
      caaggagcg cgaaggccc cctctatgc cattttgca 
      ctcgcagcc ggtcacgcc cgccaggag gcagagcat 
      tgtgacgct gcatccgcc ggagacgat ccataattc 
      tagtcagcc cagtgaagc agggccttc cagataata 
      atcactagt acctaggtt taccaaaat ggggcgctc 
      tgagttttg ataggacaa tagtggatt tttttttca 
      agactcgga gcgtgttgc taccaaaac actacctat 
      tttagctat aaatgtttt gtatttatt gatttatac 
      gtaacgata agacagaat gcgtgatat gtttaggtt 
      aaggcggaa ttaataaca ataagtgat aatttataa 
      cgcaaaagt atattatat aaatataaa atgttattt 
      acacaaaga cgttccatg ttaaatata ggaagtatg 
      aaattaaac tattccata ctgttatta tattattac 
      tacattgtt gtatacaaa tctacctcc taatttcat 
      acaaagact taataaaat atacactta actaagtat 
      ctactaact gcattttac accaaggac atcgagaaa 
      tcaattggt ggcaactcc tcaacgcca agatcatta 
      agttctgat gcataaata aagtgccaa gatcaagac 
      aaaacacac ttaaataaa aaagcaatg gtaagtaaa 
      aattgaagc tacaagggg catacaaat aagcaacac 
      ttttacgtg tatgagcgt ttttgattg ttcttagca 
      gtcagagtt aattcttta ttttttatt taacttaat 
      attttggat atcagagtc aatttttaa ataatctag 
      ccaaacaat caaattttt gaaaataca gaagaccaa 
      tcgaattat gaaatagtt aaaggactg gcaaaatga 
      gcacaattc cagttgtgt taaatgtat gtaaaataa 
      acatcaccc taaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY