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Gene information
General information
TC008149 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5818408..5820831
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008149
    iBeetle screen iB_04437

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head split - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008149 RA
      MAENRKEKF ASTYTVDNF NSTAELANN ECNIPVSGA 
      DIITDKVYV PYDHRNVEH PNTFSGALM HICKSSLGT 
      GILAIPSAV KAAGIIVGV VGTVLTGIL CTHTIHLLI 
      FASQEICKK AKLPMLGFA ETAHAVFKY GPRHIQPFA 
      NFAKNFVDF CLLLTYCSG NAVYVVFIT GNIQKVVNY 
      YQESVADWP LQYYMLILL VPLTLCCQV RQLKHLVPF 
      SIIANVTMV TAFLITLYY MFSGIGDID MEERKLFND 
      ISQFPLFFS TVIFAMEGI GTMLPIENT MIKQQFIGC 
      PGVLNFAMA FVVTLYTLI GLFGYLRFG DKVSSNVIE 
      NLPTDEIAA QVARLCVAT AVFFTFMLQ FYVPCEITW 
      RKVSSKIPK NYHNIAQIV MRTLLVLFI TAIGAAVPK 
      LDVIIGLVG SICLSTLGL FIPAAIDLT LNLGENGDF 
      GVMKWRLWK DILIAILSW FALFSGSYY AIKELIEG
    CDS sequence
    • >TC008149 RA
      atggctgaa aatcgcaaa gaaaagttc gcgtcgacg 
      tacactgtc gacaatttc aactcaacg gcagaactc 
      gccaataat gaatgcaat attccagtc agtggtgct 
      gatataatc actgataaa gtttacgtc ccgtatgac 
      cacagaaat gtggaacat cccaacaca ttttctgga 
      gcgttaatg cacatatgt aagagttct ttaggaacc 
      ggaattttg gcaatacca agtgctgtg aaagctgct 
      gggattatt gttggtgtt gttgggact gtccttaca 
      gggattttg tgtactcac acgattcat ttgctgatt 
      tttgcgtcc caagaaata tgtaagaaa gccaaactc 
      ccaatgtta ggctttgct gaaaccgcc catgctgta 
      tttaaatac ggacctagg cacattcag ccttttgca 
      aacttcgcc aaaaacttc gtagacttt tgtctcctt 
      ttgacttac tgttcagga aatgcagtt tacgttgtt 
      tttattacc ggaaacatc caaaaagtc gtgaattat 
      taccaggaa tctgtcgct gactggcca ctccagtat 
      tacatgctt atactgttg gtgccttta acattgtgt 
      tgccaagtc cgccagttg aaacacttg gtaccattt 
      tcgatcata gcaaatgtt acaatggta acagcattt 
      ttgatcact ctgtattac atgttttct gggattggt 
      gatatcgac atggaagag agaaaactc tttaatgat 
      atttcccaa tttccgctg ttcttcagc actgtgata 
      tttgcaatg gaagggatc ggcacaatg ttgccaatt 
      gaaaatacg atgattaag caacaattc atcggatgt 
      cctggtgtt ttgaacttt gcaatggcc tttgtggtc 
      actttgtat actcttatc gggctcttc ggttacctc 
      cgatttgga gataaggtc tcttcaaac gtcattgaa 
      aacttacca accgatgaa atagcagca caagtggcc 
      aggctgtgt gtagccacc gcagttttt tttacgttt 
      atgttacaa ttttacgtc ccttgtgag ataacatgg 
      aggaaagta tcgtcaaaa attccgaaa aactaccac 
      aacattgct caaattgtc atgagaacg ttactagta 
      ttatttata acagcaata ggtgcagct gttccaaaa 
      ttggacgtt attatcggg ctagtggga tcaatttgt 
      ttgtcgact cttggactt tttatacca gctgccatt 
      gatcttacc ttgaatttg ggcgaaaac ggtgatttt 
      ggggtcatg aagtggaga ttatggaaa gatattctt 
      attgctatc ttgtcgtgg tttgcgctc ttctcaggt 
      agttattat gctattaag gagttaatc gaaggttga
    mRNA sequence
    • >TC008149 RA
      ctttgcaaa aaccatggc tgaaaatcg caaagaaaa 
      gttcgcgtc gacgtacac tgtcgacaa tttcaactc 
      aacggcaga actcgccaa taatgaatg caatattcc 
      agtcagtgg tgctgatat aatcactga taaagttta 
      cgtcccgta tgaccacag aaatgtgga acatcccaa 
      cacattttc tggagcgtt aatgcacat atgtaagag 
      ttctttagg aaccggaat tttggcaat accaagtgc 
      tgtgaaagc tgctgggat tattgttgg tgttgttgg 
      gactgtcct tacagggat tttgtgtac tcacacgat 
      tcatttgct gatttttgc gtcccaaga aatatgtaa 
      gaaagccaa actcccaat gttaggctt tgctgaaac 
      cgcccatgc tgtatttaa atacggacc taggcacat 
      tcagccttt tgcaaactt cgccaaaaa cttcgtaga 
      cttttgtct ccttttgac ttactgttc aggaaatgc 
      agtttacgt tgtttttat taccggaaa catccaaaa 
      agtcgtgaa ttattacca ggaatctgt cgctgactg 
      gccactcca gtattacat gcttatact gttggtgcc 
      tttaacatt gtgttgcca agtccgcca gttgaaaca 
      cttggtacc attttcgat catagcaaa tgttacaat 
      ggtaacagc atttttgat cactctgta ttacatgtt 
      ttctgggat tggtgatat cgacatgga agagagaaa 
      actctttaa tgatatttc ccaatttcc gctgttctt 
      cagcactgt gatatttgc aatggaagg gatcggcac 
      aatgttgcc aattgaaaa tacgatgat taagcaaca 
      attcatcgg atgtcctgg tgttttgaa ctttgcaat 
      ggcctttgt ggtcacttt gtatactct tatcgggct 
      cttcggtta cctccgatt tggagataa ggtctcttc 
      aaacgtcat tgaaaactt accaaccga tgaaatagc 
      agcacaagt ggccaggct gtgtgtagc caccgcagt 
      tttttttac gtttatgtt acaatttta cgtcccttg 
      tgagataac atggaggaa agtatcgtc aaaaattcc 
      gaaaaacta ccacaacat tgctcaaat tgtcatgag 
      aacgttact agtattatt tataacagc aataggtgc 
      agctgttcc aaaattgga cgttattat cgggctagt 
      gggatcaat ttgtttgtc gactcttgg actttttat 
      accagctgc cattgatct taccttgaa tttgggcga 
      aaacggtga ttttggggt catgaagtg gagattatg 
      gaaagatat tcttattgc tatcttgtc gtggtttgc 
      gctcttctc aggtagtta ttatgctat taaggagtt 
      aatcgaagg ttgatagaa caaataaat gtgatagta 
      tttttaaat attaggttt gtcacaagc tacaaatag 
      gtaatttat tgtcataat tttaattaa cttaattga 
      gacctgtat ttttgtatt aattgtaag tgggcttct 
      gagaatgtc gtacacatt tacgatgaa taaaagaca 
      tttttaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY