Status
Please wait ...
Gene information
General information
TC008136 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5537081..5544557
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008136
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04434

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008136 RA
      MIRQIFGNL SCHVNDNKL IKIPNLNYT DSTAMSPTI 
      TCGILTVSD SCYKKEKED TSGKELERA FRRAFPSFK 
      VTHKDIIPD DRDKIINTL NKWVEDQCN IVLTTGGTG 
      FSPRDVTPE ATRAVIEKE APGIAYAMI TKSLAITPM 
      AMLSRAVAG IKAQSLIVN LPGSAKGAV ECFSFIQEC 
      IPHALALIT DNKTEVTKK HREVQKRVS FVDGCGPSK 
      VITDNVAER PRESTYAML EVDEAFELV LKESEIRVE 
      TERLAVENA LFRVLAEDV LAYDDVPPF DASVKDGYA 
      VLASDGEGP RRVRTALGA GDAPHTSPL QPGEAIRIG 
      TGAPIPPGA DAVVQVEDT SVLRKSKGE EIEINVNKA 
      PVKGQDIRR MGCDVAKNT VVLMNGERL KAPHVGVLA 
      AVGKSTVTV YKPVSVGIF TTGNELKEH YEELRPGQI 
      RDSNRLALI NLLKECHYE CDDYGIIRD DPDTIKKAI 
      ATALTINDI LITTGGVSM GEFDFIKRV LIEDFNATV 
      HFGRVNMKP GKPTTFATL SHEGAKKIV FGLPGNPVS 
      ASVTCLLFV IPLLRHLEG ANVWQWPTI PYVVSVKKQ 
      STDARPEYV RAVVSYLHG KIVAEPNGG QISSRLNSL 
      VGANGLILM PPSMIPTRE YLSHIILIG NLQT
    CDS sequence
    • >TC008136 RA
      atgattagg cagattttt ggaaacttg tcctgtcat 
      gtcaacgat aacaagttg ataaagata ccgaacctg 
      aattatact gactccaca gcaatgtct cccacaata 
      acctgcgga attctaaca gttagcgat tcctgttat 
      aaaaaggag aaggaggat acttccggc aaagaatta 
      gagcgtgct ttcaggcga gcttttccc agttttaaa 
      gttacccac aaagacata atccccgac gatcgtgac 
      aaaataatc aatacactc aacaaatgg gtcgaagac 
      cagtgcaac atcgttcta accactggc ggtaccggc 
      ttttctccc agagacgta accccggaa gcaacacgc 
      gctgtaatc gaaaaggaa gcaccaggg attgcttac 
      gccatgatt accaaaagt ctagccatc actcctatg 
      gcgatgctt tcgcgagct gtcgccggc atcaaagcc 
      caatcattg atcgtgaac ctcccgggg agtgcgaag 
      ggggcagtt gagtgtttc tctttcatc caggagtgt 
      atcccccac gctttggct ctaataaca gacaataag 
      actgaagtt acgaaaaaa cacagagaa gtccagaag 
      agggtgagc tttgttgac ggctgcggt ccgagcaaa 
      gttattaca gacaatgtg gcggagagg ccgagggaa 
      tctacttat gccatgttg gaggttgat gaagcgttt 
      gagttggtt ttgaaggaa agtgaaatt cgtgtcgag 
      acggaacga ctggcggtc gaaaacgca cttttccgg 
      gtcttggcc gaagacgta ttggcttat gatgacgtt 
      ccgccgttt gatgcgtcc gtcaaagac ggctatgct 
      gtgctggca tccgacggt gaagggccc cggagggtg 
      cgaactgct ctgggagct ggagacgct ccccacact 
      tcccccttg caacctggc gaggcaatc cgtattggc 
      accggcgct ccgatcccc ccaggagct gacgcggtc 
      gttcaagtt gaagatacg agcgttttg aggaaaagc 
      aaaggcgaa gaaatcgaa attaacgtt aacaaagcg 
      ccggtcaaa ggtcaggat atcagacgt atgggatgc 
      gatgttgct aaaaatacc gtggttttg atgaacggc 
      gaacgtttg aaagctcca catgttgga gttttggca 
      gctgtggga aaatcaaca gttacagtc tataaacca 
      gtttccgtt ggaattttt acaacgggg aacgaattg 
      aaagagcat tacgaggag ttgcgacct ggccaaatc 
      cgcgacagc aacaggctc gccttaatt aatcttctg 
      aaagaatgc cactacgag tgtgacgat tacgggata 
      atcagagat gacccggac accatcaaa aaggccatc 
      gctaccgct ttgacaatc aatgacatc ctaattaca 
      acaggggga gtgtcgatg ggggaattc gactttata 
      aaaagagtg ctaattgaa gacttcaac gcgactgtg 
      catttcggt cgcgtcaac atgaaacca ggaaaaccc 
      acaacgttt gccacttta agccacgaa ggtgcaaag 
      aagattgtt ttcggatta ccaggaaat ccggtgtcg 
      gctagtgta acatgttta ttgttcgta attccactg 
      ttacgtcac ttggaaggt gcgaatgtg tggcagtgg 
      ccaaccatt ccctatgtt gtgtccgtc aaaaagcag 
      agcaccgat gcacggcct gagtacgtc agagctgtg 
      gtttcgtac ctccacggg aaaattgtg gctgagcct 
      aatgggggg caaattagc agccgattg aacagctta 
      gtcggcgct aatggctta attttaatg ccgccgtcg 
      atgataccc acgagggag tatctttcg cacattatt 
      cttatcggg aatctacaa acgtag
    mRNA sequence
    • >TC008136 RA
      attgccttg ccttgcgta cggtgaata agtgtcgag 
      tgtggagtt ttccggcct acgcagaaa gcagcatcc 
      atcacgata ttttttccc aacatatgt atttcggac 
      ggcagcggt taagatcct gattatgaa ataatatca 
      cagtgaatg attaggcag atttttgga aacttgtcc 
      tgtcatgtc aacgataac aagttgata aagataccg 
      aacctgaat tatactgac tccacagca atgtctccc 
      acaataacc tgcggaatt ctaacagtt agcgattcc 
      tgttataaa aaggagaag gaggatact tccggcaaa 
      gaattagag cgtgctttc aggcgagct tttcccagt 
      tttaaagtt acccacaaa gacataatc cccgacgat 
      cgtgacaaa ataatcaat acactcaac aaatgggtc 
      gaagaccag tgcaacatc gttctaacc actggcggt 
      accggcttt tctcccaga gacgtaacc ccggaagca 
      acacgcgct gtaatcgaa aaggaagca ccagggatt 
      gcttacgcc atgattacc aaaagtcta gccatcact 
      cctatggcg atgctttcg cgagctgtc gccggcatc 
      aaagcccaa tcattgatc gtgaacctc ccggggagt 
      gcgaagggg gcagttgag tgtttctct ttcatccag 
      gagtgtatc ccccacgct ttggctcta ataacagac 
      aataagact gaagttacg aaaaaacac agagaagtc 
      cagaagagg gtgagcttt gttgacggc tgcggtccg 
      agcaaagtt attacagac aatgtggcg gagaggccg 
      agggaatct acttatgcc atgttggag gttgatgaa 
      gcgtttgag ttggttttg aaggaaagt gaaattcgt 
      gtcgagacg gaacgactg gcggtcgaa aacgcactt 
      ttccgggtc ttggccgaa gacgtattg gcttatgat 
      gacgttccg ccgtttgat gcgtccgtc aaagacggc 
      tatgctgtg ctggcatcc gacggtgaa gggccccgg 
      agggtgcga actgctctg ggagctgga gacgctccc 
      cacacttcc cccttgcaa cctggcgag gcaatccgt 
      attggcacc ggcgctccg atcccccca ggagctgac 
      gcggtcgtt caagttgaa gatacgagc gttttgagg 
      aaaagcaaa ggcgaagaa atcgaaatt aacgttaac 
      aaagcgccg gtcaaaggt caggatatc agacgtatg 
      ggatgcgat gttgctaaa aataccgtg gttttgatg 
      aacggcgaa cgtttgaaa gctccacat gttggagtt 
      ttggcagct gtgggaaaa tcaacagtt acagtctat 
      aaaccagtt tccgttgga atttttaca acggggaac 
      gaattgaaa gagcattac gaggagttg cgacctggc 
      caaatccgc gacagcaac aggctcgcc ttaattaat 
      cttctgaaa gaatgccac tacgagtgt gacgattac 
      gggataatc agagatgac ccggacacc atcaaaaag 
      gccatcgct accgctttg acaatcaat gacatccta 
      attacaaca gggggagtg tcgatgggg gaattcgac 
      tttataaaa agagtgcta attgaagac ttcaacgcg 
      actgtgcat ttcggtcgc gtcaacatg aaaccagga 
      aaacccaca acgtttgcc actttaagc cacgaaggt 
      gcaaagaag attgttttc ggattacca ggaaatccg 
      gtgtcggct agtgtaaca tgtttattg ttcgtaatt 
      ccactgtta cgtcacttg gaaggtgcg aatgtgtgg 
      cagtggcca accattccc tatgttgtg tccgtcaaa 
      aagcagagc accgatgca cggcctgag tacgtcaga 
      gctgtggtt tcgtacctc cacgggaaa attgtggct 
      gagcctaat ggggggcaa attagcagc cgattgaac 
      agcttagtc ggcgctaat ggcttaatt ttaatgccg 
      ccgtcgatg atacccacg agggagtat ctttcgcac 
      attattctt atcgggaat ctacaaacg tagtaattt 
      tgcatcggt tttgttggt ttttggcaa gggtttttt 
      gtacttttg taatgattt cctattttt tttttgaat 
      aaacttttt ttgcatgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY