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Gene information
General information
TC008135 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5501586..5506830
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008135
    iBeetle screen iB_04433

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape rounded - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer rounded - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    pygopodium not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008135 RA
      MLAKKADVV LVLLIQIIL IVLFFIFTT YDDKYESNA 
      YPMFQDIHV MIFIGFGFL MTFLRRYGY SAVGFNFLL 
      GALIIQWAL LCQGFYQLN ENNKMKLGI GSLYKADIA 
      AATVLISMG AVLGRTSYM QLLIMGIVE IAVFSANSF 
      LGSNIFQVA DAGGSMFVH AFGAYFGLA VSYVIELKK 
      AKTITDNGL EESRYTSDL FAMIGTIFL WLYWPSFNA 
      VELTGDGQQ RAVMNTYLS LASCCVTTF AVSLIFSKN 
      HKFDMVHVQ NSTLAGGVA VGTTANLML QPFGAVLIG 
      AVAGVVSVA GYAKLTPWL SSNFGIRDT CGVHNLHGI 
      PGVLAGLIG AFMAGIASE ATYCKTLYE IYPARASPN 
      MTATAEYTF LKPGLGRDE AQQAGYQTL ALVVTVTLA 
      VASGLLTGM LVMWENFLP LKEECFDDR HAWHLPRKK 
      AEIGSMVTF KPMIKNNYY SVFERVL
    CDS sequence
    • >TC008135 RA
      atgctcgcc aagaaagca gacgttgtg ctagtcctt 
      ctgattcaa attatcctc attgtgcta ttttttatt 
      ttcaccact tatgatgat aaatacgaa tccaatgcc 
      tacccgatg tttcaggac attcacgtg atgattttt 
      atcgggttt ggattcctt atgacattt ttacgacga 
      tatggttac agtgcagtg ggttttaat tttttattg 
      ggcgctttg attatccaa tgggctttg ctttgtcag 
      ggcttttac cagctcaat gagaataat aaaatgaag 
      ctcggaatt ggaagttta tacaaagct gatattgcc 
      gcggccact gtgctcatt tccatgggg gcggttttg 
      gggcgcacc tcttacatg cagctcctg atcatggga 
      attgtcgaa atcgccgtt ttttcggcc aattctttt 
      ctaggatcg aacatattt caggtagcg gacgctgga 
      ggatcgatg tttgtccat gcatttggt gcctatttc 
      ggcttggcc gttagttac gtaattgaa ctaaaaaaa 
      gcgaaaaca atcactgat aacggcctc gaagaatcg 
      agatacacg tccgatctt ttcgcaatg attggaaca 
      atctttctt tggctttat tggcccagt ttcaacgcc 
      gttgagcta acaggagac ggccagcaa agggccgtt 
      atgaatact tatctatct ctggcttcc tgctgtgtc 
      acgactttc gccgtctcg ctcatcttc agcaaaaac 
      cacaagttc gacatggtg cacgtgcaa aactccacg 
      ctcgcaggt ggtgtggcc gtcggcacg accgccaac 
      ctcatgctg caacccttc ggtgctgtg ctcattggg 
      gccgtcgca ggcgtcgtc tcggtggcg ggttacgcc 
      aagcttaca ccttggttg agttcaaac ttcggaatt 
      agagatact tgtggcgtt cataacctt cacggcatt 
      cctggagta ttagcgggt ctaattggg gcattcatg 
      gccggaata gcttcagaa gccacctac tgtaaaact 
      ctatacgag atataccca gccagagcg agcccaaat 
      atgacagca actgccgaa tacaccttc ctgaagccg 
      ggactcggg cgagacgag gcccaacaa gccggttac 
      caaacacta gctcttgta gttacggtt actctagcg 
      gtcgcatcc gggttactt acagggatg cttgtaatg 
      tgggaaaac tttctacca ctgaaagag gaatgtttc 
      gacgatcga cacgcgtgg catttgccg agaaaaaag 
      gccgaaatt gggtcaatg gtcacgttt aaacccatg 
      attaaaaat aattactac agtgtgttt gaaagggta 
      ttgtag
    mRNA sequence
    • >TC008135 RA
      atgtaaatt atgaaatag ttctggaac gaactttgc 
      acgataata aagttgttt ttcaccgcg agtcgatcg 
      ttcatcatg ctcgccaag aaagcagac gttgtgcta 
      gtccttctg attcaaatt atcctcatt gtgctattt 
      tttattttc accacttat gatgataaa tacgaatcc 
      aatgcctac ccgatgttt caggacatt cacgtgatg 
      atttttatc gggtttgga ttccttatg acattttta 
      cgacgatat ggttacagt gcagtgggt tttaatttt 
      ttattgggc gctttgatt atccaatgg gctttgctt 
      tgtcagggc ttttaccag ctcaatgag aataataaa 
      atgaagctc ggaattgga agtttatac aaagctgat 
      attgccgcg gccactgtg ctcatttcc atgggggcg 
      gttttgggg cgcacctct tacatgcag ctcctgatc 
      atgggaatt gtcgaaatc gccgttttt tcggccaat 
      tcttttcta ggatcgaac atatttcag gtagcggac 
      gctggagga tcgatgttt gtccatgca tttggtgcc 
      tatttcggc ttggccgtt agttacgta attgaacta 
      aaaaaagcg aaaacaatc actgataac ggcctcgaa 
      gaatcgaga tacacgtcc gatcttttc gcaatgatt 
      ggaacaatc tttctttgg ctttattgg cccagtttc 
      aacgccgtt gagctaaca ggagacggc cagcaaagg 
      gccgttatg aatacttat ctatctctg gcttcctgc 
      tgtgtcacg actttcgcc gtctcgctc atcttcagc 
      aaaaaccac aagttcgac atggtgcac gtgcaaaac 
      tccacgctc gcaggtggt gtggccgtc ggcacgacc 
      gccaacctc atgctgcaa cccttcggt gctgtgctc 
      attggggcc gtcgcaggc gtcgtctcg gtggcgggt 
      tacgccaag cttacacct tggttgagt tcaaacttc 
      ggaattaga gatacttgt ggcgttcat aaccttcac 
      ggcattcct ggagtatta gcgggtcta attggggca 
      ttcatggcc ggaatagct tcagaagcc acctactgt 
      aaaactcta tacgagata tacccagcc agagcgagc 
      ccaaatatg acagcaact gccgaatac accttcctg 
      aagccggga ctcgggcga gacgaggcc caacaagcc 
      ggttaccaa acactagct cttgtagtt acggttact 
      ctagcggtc gcatccggg ttacttaca gggatgctt 
      gtaatgtgg gaaaacttt ctaccactg aaagaggaa 
      tgtttcgac gatcgacac gcgtggcat ttgccgaga 
      aaaaaggcc gaaattggg tcaatggtc acgtttaaa 
      cccatgatt aaaaataat tactacagt gtgtttgaa 
      agggtattg tagaaaatt gttgtttca ctccctaga 
      ttccggcta gactgaccc tctctaatt gtactgcac 
      ttaccctca atttatttt cccgcactt tcaaattat 
      gtaaatatg tgcaaatta cgcaactat ttggctttg 
      tagttacgg ctctagtgc catatggca gatttattt 
      gcgatttga tttaattat ccccgataa aaaaaattc 
      gtaacaatt tcaataatt tttattcat tgttgttac 
      aatacaatt tgcaggact tattctaaa gttgacaaa 
      agttgggac aatttcaag cgagagctt tcataacga 
      cgccacctc ttggatact cccggaaca gaacgtgaa 
      agctcggca ggacccgct ggggtgcca ctacgacaa 
      ccccagttt tgaacttcc agccccgac tgcagatgt 
      ctatagtta acacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY