Status
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Gene information
General information
TC008134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5427326..5498084
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008134
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008134 RA
      MRCSQRLIL LLIATSVCM GATSTSWKK KERKREKVK 
SQRDANLCT MEGNAAALH CVCVGGSPP VNTTSAECW 
VFTGVSKDH SIWSLFATQ PHITQLKFL VRPQGQFTF 
VPTKGLRHL NHLKELEIM YASINEIHP YAFANSSSL 
RELTLSRNQ IVVLDHHAF AHLTNLTEL SLEENRIAE 
LRRDVFVDL PRLRKLYAA HNNLSVIQE GAFRHLAHL 
LELELDTNY ISVLTRDTF TGLGELKRL DLSYNKISM 
LGDLTFSEL WVLEELSLE YNQIELISN RAFAGLNHL 
RKLSLSHNR LAVLSGGLL GGVPTIIYL DLRSNYLET 
ITYDSVQPI IHNLKNESS HFYIADNKF VCDCRLAWL 
HNLRNETKN PSIRNTLDE ITCYMESPG QSLARGTVP 
SPTTAPRTV DNQFPENAE DYKYDYETV EYDNDIYDQ 
QQDSSLNYV TASGPKDPR KRHLFEIPL QELPCPQQE 
HPTEAPRTL LHTPGYAGI NASGAPRLL GALGLVLSA 
MFLNMT
    CDS sequence
    • >TC008134 RA
      atgagatgt tcccaacgt ctgatatta ttgctaatt 
gcgacgtca gtgtgcatg ggtgcgacg tcgaccagt 
tggaaaaaa aaggagcgg aagagggaa aaggtgaag 
tctcaaaga gatgcaaat ctttgcaca atggaagga 
aacgcggcc gctttgcat tgcgtttgt gtgggaggg 
tcgccccct gtcaacacg acgagcgcc gagtgctgg 
gtgttcacc ggagtctcc aaagatcac agcatatgg 
tccctgttt gcgactcag ccgcacatc acccagctg 
aagttcctc gtcaggccg caaggacaa ttcaccttc 
gtacccacc aaaggattg cggcacttg aaccacctc 
aaagagctg gagataatg tacgccagc ataaacgag 
attcacccg tacgccttt gcgaatagt tcgtctttg 
cgggaatta acgctgtcg aggaaccag atcgtggtg 
cttgatcac cacgccttt gcgcatctt accaacttg 
acggagctg tccttggaa gagaacagg atagcggag 
ctacgtaga gacgtgttt gtcgatttg ccacgcttg 
cggaagctt tacgcggct cacaacaat ttgagtgtg 
atacaagaa ggggcattt agacatctc gctcatctc 
ttggagctg gagctggac acgaactat ataagcgtg 
ctgaccaga gacacgttt acaggactc ggcgagctg 
aaaaggctt gatctcagc tataacaaa atcagcatg 
ctcggagat ctcactttc tcggagttg tgggttctg 
gaggagctc agtttggaa tacaaccag attgagttg 
atttcaaat cgcgcattc gctggccta aatcatctg 
cgaaaactc agcctgagc cataatcgt ttggctgtt 
ctgtcaggg ggactttta ggaggcgtt cccaccata 
atctatcta gatctacga tccaactat ctcgagacg 
attacatac gacagcgta cagcccatc attcataat 
ctgaaaaat gagagcagc cacttttac atcgcagat 
aacaaattc gtctgtgac tgtcgtctg gcctggctg 
cacaattta cgtaacgaa acgaaaaat ccgtcgatc 
cgaaacacc ctggacgag atcacatgt tatatggaa 
tctccgggt caaagttta gcccggggc acagtgccc 
tcgccgacc accgctccc agaacagtc gataaccaa 
tttcctgaa aacgctgaa gactacaaa tacgactac 
gagaccgtc gaatacgac aacgacatc tacgatcag 
cagcaggac agctccttg aattacgtg acggcatcc 
ggtcccaag gaccccagg aagcggcat ctattcgag 
attccgctg caggaattg ccctgcccg cagcaggag 
catccgacc gaggcgccc aggaccctt ctgcacacc 
ccgggatac gcagggatc aacgccagc ggcgccccc 
aggctcctg ggcgccctc ggcttagtg ctaagcgcc 
atgttcttg aacatgacg taa
    mRNA sequence
    • >TC008134 RA
      ttgctttga atatatgcc gcctgcagt ttattatgg 
gacatgtat ataaacagt cttctctaa acgactttc 
ccgactaat aaagtagcc attcataaa gttttgtga 
agtttcaaa ctcacataa actgtgtat tttcgtaat 
caaataaga gctttcatc agatataag gggaggata 
aatttaaat taaatttta gtgcgttta cgtggctgt 
gtttatgtc atctgtgcg aggggaaag tgtgtcgtc 
accatgaga tgttcccaa cgtctgata ttattgcta 
attgcgacg tcagtgtgc atgggtgcg acgtcgacc 
agttggaaa aaaaaggag cggaagagg gaaaaggtg 
aagtctcaa agagatgca aatctttgc acaatggaa 
ggaaacgcg gccgctttg cattgcgtt tgtgtggga 
gggtcgccc cctgtcaac acgacgagc gccgagtgc 
tgggtgttc accggagtc tccaaagat cacagcata 
tggtccctg tttgcgact cagccgcac atcacccag 
ctgaagttc ctcgtcagg ccgcaagga caattcacc 
ttcgtaccc accaaagga ttgcggcac ttgaaccac 
ctcaaagag ctggagata atgtacgcc agcataaac 
gagattcac ccgtacgcc tttgcgaat agttcgtct 
ttgcgggaa ttaacgctg tcgaggaac cagatcgtg 
gtgcttgat caccacgcc tttgcgcat cttaccaac 
ttgacggag ctgtccttg gaagagaac aggatagcg 
gagctacgt agagacgtg tttgtcgat ttgccacgc 
ttgcggaag ctttacgcg gctcacaac aatttgagt 
gtgatacaa gaaggggca tttagacat ctcgctcat 
ctcttggag ctggagctg gacacgaac tatataagc 
gtgctgacc agagacacg tttacagga ctcggcgag 
ctgaaaagg cttgatctc agctataac aaaatcagc 
atgctcgga gatctcact ttctcggag ttgtgggtt 
ctggaggag ctcagtttg gaatacaac cagattgag 
ttgatttca aatcgcgca ttcgctggc ctaaatcat 
ctgcgaaaa ctcagcctg agccataat cgtttggct 
gttctgtca gggggactt ttaggaggc gttcccacc 
ataatctat ctagatcta cgatccaac tatctcgag 
acgattaca tacgacagc gtacagccc atcattcat 
aatctgaaa aatgagagc agccacttt tacatcgca 
gataacaaa ttcgtctgt gactgtcgt ctggcctgg 
ctgcacaat ttacgtaac gaaacgaaa aatccgtcg 
atccgaaac accctggac gagatcaca tgttatatg 
gaatctccg ggtcaaagt ttagcccgg ggcacagtg 
ccctcgccg accaccgct cccagaaca gtcgataac 
caatttcct gaaaacgct gaagactac aaatacgac 
tacgagacc gtcgaatac gacaacgac atctacgat 
cagcagcag gacagctcc ttgaattac gtgacggca 
tccggtccc aaggacccc aggaagcgg catctattc 
gagattccg ctgcaggaa ttgccctgc ccgcagcag 
gagcatccg accgaggcg cccaggacc cttctgcac 
accccggga tacgcaggg atcaacgcc agcggcgcc 
cccaggctc ctgggcgcc ctcggctta gtgctaagc 
gccatgttc ttgaacatg acgtaaaat cggcaagga 
ctggtcgag tgtggctgt gtacaggaa tcattgtgt 
gggagtttg atcgagtac aacgcgcaa aaggacggt 
tttaggtca gattgtgcc cattgttct aaagactgt 
tagtcctac tctacacaa ccattttac aaccctccg 
atcaccatc acgtcgcat atcgtaact gtagctcaa 
ttcccggtg tacatggaa cgcattttt ttaaatcaa 
actcaaagt cggtcaatt tcggttgtt cctaagtgg 
tacctaacg tccaatttt ttaacactt cttgcagat 
ttcaattac agtaacgtt ccattatta atgtaaata 
ttaagtgta ataacatgg ctgtttaat atcacgatt 
tttgaaata gtttctgga tgtactcac acagaagtt 
tataagtta gcttagact aacgaacct aaacctaga 
aaatatttt gtaaattat tgagaaaag cctagtctt 
taagtgtat ttttaaata tgtcgattc gcactgatg 
taactaatg ccattaatt aataagcaa ctattttga 
aatattaag gttaaaatg tgtaattat aatgtaaaa 
tctaatgtt aattcgttg taatttgta atgtagcag 
tcaatgaag ctaagaata tttcacatc taggacgcc 
taaagaacg aattgtgtt aaaaaaaaa atttttttg 
ctccctcgt taacgaaag tcaacgctc aaaacagtt 
ccatcatcc aattaataa aaaaattct taatttaaa 
aaagttgga gtgagattc tgtacgagt tgtgaatat 
atttcggaa tatttttgt ggctttcgg tcttggttt 
tgttgagcc aaacgtcct gttctgcac acaacatgt 
attttgaga tttgtacgt atgtgttaa actgtgtat 
ttcgcttat tctaaaatc tatactcat aagatgtct 
attccctta taatttttg taaatatct ttcatgtga 
ttttcattg aaaataaaa ttctaaatc gagc

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY