| General information | |||
| TC008134 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 5427326..5498084 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008134 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04432 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC008134 RA
MRCSQRLIL LLIATSVCM GATSTSWKK KERKREKVK SQRDANLCT MEGNAAALH CVCVGGSPP VNTTSAECW VFTGVSKDH SIWSLFATQ PHITQLKFL VRPQGQFTF VPTKGLRHL NHLKELEIM YASINEIHP YAFANSSSL RELTLSRNQ IVVLDHHAF AHLTNLTEL SLEENRIAE LRRDVFVDL PRLRKLYAA HNNLSVIQE GAFRHLAHL LELELDTNY ISVLTRDTF TGLGELKRL DLSYNKISM LGDLTFSEL WVLEELSLE YNQIELISN RAFAGLNHL RKLSLSHNR LAVLSGGLL GGVPTIIYL DLRSNYLET ITYDSVQPI IHNLKNESS HFYIADNKF VCDCRLAWL HNLRNETKN PSIRNTLDE ITCYMESPG QSLARGTVP SPTTAPRTV DNQFPENAE DYKYDYETV EYDNDIYDQ QQDSSLNYV TASGPKDPR KRHLFEIPL QELPCPQQE HPTEAPRTL LHTPGYAGI NASGAPRLL GALGLVLSA MFLNMT
- >TC008134 RA
atgagatgt tcccaacgt ctgatatta ttgctaatt gcgacgtca gtgtgcatg ggtgcgacg tcgaccagt tggaaaaaa aaggagcgg aagagggaa aaggtgaag tctcaaaga gatgcaaat ctttgcaca atggaagga aacgcggcc gctttgcat tgcgtttgt gtgggaggg tcgccccct gtcaacacg acgagcgcc gagtgctgg gtgttcacc ggagtctcc aaagatcac agcatatgg tccctgttt gcgactcag ccgcacatc acccagctg aagttcctc gtcaggccg caaggacaa ttcaccttc gtacccacc aaaggattg cggcacttg aaccacctc aaagagctg gagataatg tacgccagc ataaacgag attcacccg tacgccttt gcgaatagt tcgtctttg cgggaatta acgctgtcg aggaaccag atcgtggtg cttgatcac cacgccttt gcgcatctt accaacttg acggagctg tccttggaa gagaacagg atagcggag ctacgtaga gacgtgttt gtcgatttg ccacgcttg cggaagctt tacgcggct cacaacaat ttgagtgtg atacaagaa ggggcattt agacatctc gctcatctc ttggagctg gagctggac acgaactat ataagcgtg ctgaccaga gacacgttt acaggactc ggcgagctg aaaaggctt gatctcagc tataacaaa atcagcatg ctcggagat ctcactttc tcggagttg tgggttctg gaggagctc agtttggaa tacaaccag attgagttg atttcaaat cgcgcattc gctggccta aatcatctg cgaaaactc agcctgagc cataatcgt ttggctgtt ctgtcaggg ggactttta ggaggcgtt cccaccata atctatcta gatctacga tccaactat ctcgagacg attacatac gacagcgta cagcccatc attcataat ctgaaaaat gagagcagc cacttttac atcgcagat aacaaattc gtctgtgac tgtcgtctg gcctggctg cacaattta cgtaacgaa acgaaaaat ccgtcgatc cgaaacacc ctggacgag atcacatgt tatatggaa tctccgggt caaagttta gcccggggc acagtgccc tcgccgacc accgctccc agaacagtc gataaccaa tttcctgaa aacgctgaa gactacaaa tacgactac gagaccgtc gaatacgac aacgacatc tacgatcag cagcaggac agctccttg aattacgtg acggcatcc ggtcccaag gaccccagg aagcggcat ctattcgag attccgctg caggaattg ccctgcccg cagcaggag catccgacc gaggcgccc aggaccctt ctgcacacc ccgggatac gcagggatc aacgccagc ggcgccccc aggctcctg ggcgccctc ggcttagtg ctaagcgcc atgttcttg aacatgacg taa
- >TC008134 RA
ttgctttga atatatgcc gcctgcagt ttattatgg gacatgtat ataaacagt cttctctaa acgactttc ccgactaat aaagtagcc attcataaa gttttgtga agtttcaaa ctcacataa actgtgtat tttcgtaat caaataaga gctttcatc agatataag gggaggata aatttaaat taaatttta gtgcgttta cgtggctgt gtttatgtc atctgtgcg aggggaaag tgtgtcgtc accatgaga tgttcccaa cgtctgata ttattgcta attgcgacg tcagtgtgc atgggtgcg acgtcgacc agttggaaa aaaaaggag cggaagagg gaaaaggtg aagtctcaa agagatgca aatctttgc acaatggaa ggaaacgcg gccgctttg cattgcgtt tgtgtggga gggtcgccc cctgtcaac acgacgagc gccgagtgc tgggtgttc accggagtc tccaaagat cacagcata tggtccctg tttgcgact cagccgcac atcacccag ctgaagttc ctcgtcagg ccgcaagga caattcacc ttcgtaccc accaaagga ttgcggcac ttgaaccac ctcaaagag ctggagata atgtacgcc agcataaac gagattcac ccgtacgcc tttgcgaat agttcgtct ttgcgggaa ttaacgctg tcgaggaac cagatcgtg gtgcttgat caccacgcc tttgcgcat cttaccaac ttgacggag ctgtccttg gaagagaac aggatagcg gagctacgt agagacgtg tttgtcgat ttgccacgc ttgcggaag ctttacgcg gctcacaac aatttgagt gtgatacaa gaaggggca tttagacat ctcgctcat ctcttggag ctggagctg gacacgaac tatataagc gtgctgacc agagacacg tttacagga ctcggcgag ctgaaaagg cttgatctc agctataac aaaatcagc atgctcgga gatctcact ttctcggag ttgtgggtt ctggaggag ctcagtttg gaatacaac cagattgag ttgatttca aatcgcgca ttcgctggc ctaaatcat ctgcgaaaa ctcagcctg agccataat cgtttggct gttctgtca gggggactt ttaggaggc gttcccacc ataatctat ctagatcta cgatccaac tatctcgag acgattaca tacgacagc gtacagccc atcattcat aatctgaaa aatgagagc agccacttt tacatcgca gataacaaa ttcgtctgt gactgtcgt ctggcctgg ctgcacaat ttacgtaac gaaacgaaa aatccgtcg atccgaaac accctggac gagatcaca tgttatatg gaatctccg ggtcaaagt ttagcccgg ggcacagtg ccctcgccg accaccgct cccagaaca gtcgataac caatttcct gaaaacgct gaagactac aaatacgac tacgagacc gtcgaatac gacaacgac atctacgat cagcagcag gacagctcc ttgaattac gtgacggca tccggtccc aaggacccc aggaagcgg catctattc gagattccg ctgcaggaa ttgccctgc ccgcagcag gagcatccg accgaggcg cccaggacc cttctgcac accccggga tacgcaggg atcaacgcc agcggcgcc cccaggctc ctgggcgcc ctcggctta gtgctaagc gccatgttc ttgaacatg acgtaaaat cggcaagga ctggtcgag tgtggctgt gtacaggaa tcattgtgt gggagtttg atcgagtac aacgcgcaa aaggacggt tttaggtca gattgtgcc cattgttct aaagactgt tagtcctac tctacacaa ccattttac aaccctccg atcaccatc acgtcgcat atcgtaact gtagctcaa ttcccggtg tacatggaa cgcattttt ttaaatcaa actcaaagt cggtcaatt tcggttgtt cctaagtgg tacctaacg tccaatttt ttaacactt cttgcagat ttcaattac agtaacgtt ccattatta atgtaaata ttaagtgta ataacatgg ctgtttaat atcacgatt tttgaaata gtttctgga tgtactcac acagaagtt tataagtta gcttagact aacgaacct aaacctaga aaatatttt gtaaattat tgagaaaag cctagtctt taagtgtat ttttaaata tgtcgattc gcactgatg taactaatg ccattaatt aataagcaa ctattttga aatattaag gttaaaatg tgtaattat aatgtaaaa tctaatgtt aattcgttg taatttgta atgtagcag tcaatgaag ctaagaata tttcacatc taggacgcc taaagaacg aattgtgtt aaaaaaaaa atttttttg ctccctcgt taacgaaag tcaacgctc aaaacagtt ccatcatcc aattaataa aaaaattct taatttaaa aaagttgga gtgagattc tgtacgagt tgtgaatat atttcggaa tatttttgt ggctttcgg tcttggttt tgttgagcc aaacgtcct gttctgcac acaacatgt attttgaga tttgtacgt atgtgttaa actgtgtat ttcgcttat tctaaaatc tatactcat aagatgtct attccctta taatttttg taaatatct ttcatgtga ttttcattg aaaataaaa ttctaaatc gagc
