Status
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Gene information
General information
TC008134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5427326..5498084
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008134
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04432

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008134 RA
      MRCSQRLIL LLIATSVCM GATSTSWKK KERKREKVK 
      SQRDANLCT MEGNAAALH CVCVGGSPP VNTTSAECW 
      VFTGVSKDH SIWSLFATQ PHITQLKFL VRPQGQFTF 
      VPTKGLRHL NHLKELEIM YASINEIHP YAFANSSSL 
      RELTLSRNQ IVVLDHHAF AHLTNLTEL SLEENRIAE 
      LRRDVFVDL PRLRKLYAA HNNLSVIQE GAFRHLAHL 
      LELELDTNY ISVLTRDTF TGLGELKRL DLSYNKISM 
      LGDLTFSEL WVLEELSLE YNQIELISN RAFAGLNHL 
      RKLSLSHNR LAVLSGGLL GGVPTIIYL DLRSNYLET 
      ITYDSVQPI IHNLKNESS HFYIADNKF VCDCRLAWL 
      HNLRNETKN PSIRNTLDE ITCYMESPG QSLARGTVP 
      SPTTAPRTV DNQFPENAE DYKYDYETV EYDNDIYDQ 
      QQDSSLNYV TASGPKDPR KRHLFEIPL QELPCPQQE 
      HPTEAPRTL LHTPGYAGI NASGAPRLL GALGLVLSA 
      MFLNMT
    CDS sequence
    • >TC008134 RA
      atgagatgt tcccaacgt ctgatatta ttgctaatt 
      gcgacgtca gtgtgcatg ggtgcgacg tcgaccagt 
      tggaaaaaa aaggagcgg aagagggaa aaggtgaag 
      tctcaaaga gatgcaaat ctttgcaca atggaagga 
      aacgcggcc gctttgcat tgcgtttgt gtgggaggg 
      tcgccccct gtcaacacg acgagcgcc gagtgctgg 
      gtgttcacc ggagtctcc aaagatcac agcatatgg 
      tccctgttt gcgactcag ccgcacatc acccagctg 
      aagttcctc gtcaggccg caaggacaa ttcaccttc 
      gtacccacc aaaggattg cggcacttg aaccacctc 
      aaagagctg gagataatg tacgccagc ataaacgag 
      attcacccg tacgccttt gcgaatagt tcgtctttg 
      cgggaatta acgctgtcg aggaaccag atcgtggtg 
      cttgatcac cacgccttt gcgcatctt accaacttg 
      acggagctg tccttggaa gagaacagg atagcggag 
      ctacgtaga gacgtgttt gtcgatttg ccacgcttg 
      cggaagctt tacgcggct cacaacaat ttgagtgtg 
      atacaagaa ggggcattt agacatctc gctcatctc 
      ttggagctg gagctggac acgaactat ataagcgtg 
      ctgaccaga gacacgttt acaggactc ggcgagctg 
      aaaaggctt gatctcagc tataacaaa atcagcatg 
      ctcggagat ctcactttc tcggagttg tgggttctg 
      gaggagctc agtttggaa tacaaccag attgagttg 
      atttcaaat cgcgcattc gctggccta aatcatctg 
      cgaaaactc agcctgagc cataatcgt ttggctgtt 
      ctgtcaggg ggactttta ggaggcgtt cccaccata 
      atctatcta gatctacga tccaactat ctcgagacg 
      attacatac gacagcgta cagcccatc attcataat 
      ctgaaaaat gagagcagc cacttttac atcgcagat 
      aacaaattc gtctgtgac tgtcgtctg gcctggctg 
      cacaattta cgtaacgaa acgaaaaat ccgtcgatc 
      cgaaacacc ctggacgag atcacatgt tatatggaa 
      tctccgggt caaagttta gcccggggc acagtgccc 
      tcgccgacc accgctccc agaacagtc gataaccaa 
      tttcctgaa aacgctgaa gactacaaa tacgactac 
      gagaccgtc gaatacgac aacgacatc tacgatcag 
      cagcaggac agctccttg aattacgtg acggcatcc 
      ggtcccaag gaccccagg aagcggcat ctattcgag 
      attccgctg caggaattg ccctgcccg cagcaggag 
      catccgacc gaggcgccc aggaccctt ctgcacacc 
      ccgggatac gcagggatc aacgccagc ggcgccccc 
      aggctcctg ggcgccctc ggcttagtg ctaagcgcc 
      atgttcttg aacatgacg taa
    mRNA sequence
    • >TC008134 RA
      ttgctttga atatatgcc gcctgcagt ttattatgg 
      gacatgtat ataaacagt cttctctaa acgactttc 
      ccgactaat aaagtagcc attcataaa gttttgtga 
      agtttcaaa ctcacataa actgtgtat tttcgtaat 
      caaataaga gctttcatc agatataag gggaggata 
      aatttaaat taaatttta gtgcgttta cgtggctgt 
      gtttatgtc atctgtgcg aggggaaag tgtgtcgtc 
      accatgaga tgttcccaa cgtctgata ttattgcta 
      attgcgacg tcagtgtgc atgggtgcg acgtcgacc 
      agttggaaa aaaaaggag cggaagagg gaaaaggtg 
      aagtctcaa agagatgca aatctttgc acaatggaa 
      ggaaacgcg gccgctttg cattgcgtt tgtgtggga 
      gggtcgccc cctgtcaac acgacgagc gccgagtgc 
      tgggtgttc accggagtc tccaaagat cacagcata 
      tggtccctg tttgcgact cagccgcac atcacccag 
      ctgaagttc ctcgtcagg ccgcaagga caattcacc 
      ttcgtaccc accaaagga ttgcggcac ttgaaccac 
      ctcaaagag ctggagata atgtacgcc agcataaac 
      gagattcac ccgtacgcc tttgcgaat agttcgtct 
      ttgcgggaa ttaacgctg tcgaggaac cagatcgtg 
      gtgcttgat caccacgcc tttgcgcat cttaccaac 
      ttgacggag ctgtccttg gaagagaac aggatagcg 
      gagctacgt agagacgtg tttgtcgat ttgccacgc 
      ttgcggaag ctttacgcg gctcacaac aatttgagt 
      gtgatacaa gaaggggca tttagacat ctcgctcat 
      ctcttggag ctggagctg gacacgaac tatataagc 
      gtgctgacc agagacacg tttacagga ctcggcgag 
      ctgaaaagg cttgatctc agctataac aaaatcagc 
      atgctcgga gatctcact ttctcggag ttgtgggtt 
      ctggaggag ctcagtttg gaatacaac cagattgag 
      ttgatttca aatcgcgca ttcgctggc ctaaatcat 
      ctgcgaaaa ctcagcctg agccataat cgtttggct 
      gttctgtca gggggactt ttaggaggc gttcccacc 
      ataatctat ctagatcta cgatccaac tatctcgag 
      acgattaca tacgacagc gtacagccc atcattcat 
      aatctgaaa aatgagagc agccacttt tacatcgca 
      gataacaaa ttcgtctgt gactgtcgt ctggcctgg 
      ctgcacaat ttacgtaac gaaacgaaa aatccgtcg 
      atccgaaac accctggac gagatcaca tgttatatg 
      gaatctccg ggtcaaagt ttagcccgg ggcacagtg 
      ccctcgccg accaccgct cccagaaca gtcgataac 
      caatttcct gaaaacgct gaagactac aaatacgac 
      tacgagacc gtcgaatac gacaacgac atctacgat 
      cagcagcag gacagctcc ttgaattac gtgacggca 
      tccggtccc aaggacccc aggaagcgg catctattc 
      gagattccg ctgcaggaa ttgccctgc ccgcagcag 
      gagcatccg accgaggcg cccaggacc cttctgcac 
      accccggga tacgcaggg atcaacgcc agcggcgcc 
      cccaggctc ctgggcgcc ctcggctta gtgctaagc 
      gccatgttc ttgaacatg acgtaaaat cggcaagga 
      ctggtcgag tgtggctgt gtacaggaa tcattgtgt 
      gggagtttg atcgagtac aacgcgcaa aaggacggt 
      tttaggtca gattgtgcc cattgttct aaagactgt 
      tagtcctac tctacacaa ccattttac aaccctccg 
      atcaccatc acgtcgcat atcgtaact gtagctcaa 
      ttcccggtg tacatggaa cgcattttt ttaaatcaa 
      actcaaagt cggtcaatt tcggttgtt cctaagtgg 
      tacctaacg tccaatttt ttaacactt cttgcagat 
      ttcaattac agtaacgtt ccattatta atgtaaata 
      ttaagtgta ataacatgg ctgtttaat atcacgatt 
      tttgaaata gtttctgga tgtactcac acagaagtt 
      tataagtta gcttagact aacgaacct aaacctaga 
      aaatatttt gtaaattat tgagaaaag cctagtctt 
      taagtgtat ttttaaata tgtcgattc gcactgatg 
      taactaatg ccattaatt aataagcaa ctattttga 
      aatattaag gttaaaatg tgtaattat aatgtaaaa 
      tctaatgtt aattcgttg taatttgta atgtagcag 
      tcaatgaag ctaagaata tttcacatc taggacgcc 
      taaagaacg aattgtgtt aaaaaaaaa atttttttg 
      ctccctcgt taacgaaag tcaacgctc aaaacagtt 
      ccatcatcc aattaataa aaaaattct taatttaaa 
      aaagttgga gtgagattc tgtacgagt tgtgaatat 
      atttcggaa tatttttgt ggctttcgg tcttggttt 
      tgttgagcc aaacgtcct gttctgcac acaacatgt 
      attttgaga tttgtacgt atgtgttaa actgtgtat 
      ttcgcttat tctaaaatc tatactcat aagatgtct 
      attccctta taatttttg taaatatct ttcatgtga 
      ttttcattg aaaataaaa ttctaaatc gagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY