Status
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Gene information
General information
TC008131 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5382666..5384794
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008131
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04431

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    only 4 eggs on the slide  
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008131 RA
      MASTLFFGS ESQSITNLD AKKIRQSPH AIVVNDFSI 
      QTGILYKNK EVLENPIYW HKFIVNNAE DFSRDLVLK 
      TILDHVQPM DLIPVFFLK EGSSASFLA RNCGAAIEK 
      ICKDNLIID NPYVKNQPF KLTIILRFS TTNDVKIDV 
      SKNISKVLR KRTNPATAT INLENFYQD SDLTEFCIL 
      SQPKLMSYV LHLSRLAKP KAIILSNNE IRSLVPIEA 
      LWGINVTSL DLRNNLIMS LTELEPLTQ LKITELWLE 
      GNPLCDNYD EHTYIQRVK EYCPKIERL DGVLLKHNG 
      FPSFRRNFL CNSCGHNLV DQFLDYYFT SYDSSNRGM 
      LKNLYHKGA LFSLTADYH STQLSSASA QLKRYKTHS 
      RNLLSLADL SRSDLNLQQ GSKAIIDLL NLLPPSEHD 
      PYSFTVDLI HYSDVCAIV VVTGVFKEL PETILDAER 
      FMGFNRCFA LESINGEYC IINEQMHVF NALTSQQVK 
      AFKHERPIR PQMLPPAQT AKQKSQVIT AMKLITNLT 
      TEWAKKCLE ECHYDFKSA LALFVDLYK VDKIPADGF 
      QN
    CDS sequence
    • >TC008131 RA
      atggcttca acgctgttt tttggtagc gagtcccag 
      tcgataacg aatttggac gcgaaaaaa attcgccag 
      agtccgcac gctattgtc gttaacgat ttttcgata 
      cagactggg attctctac aaaaataag gaagttctg 
      gaaaacccc atctactgg cataagttt attgtgaat 
      aacgccgag gattttagc cgggacttg gttttgaaa 
      acaattctg gaccatgtg caaccgatg gatttaatc 
      ccggttttc ttcctcaaa gaaggcagt tctgcttcg 
      ttccttgcg cggaactgt ggagcagcg attgaaaaa 
      atatgtaaa gataatttg attattgat aacccgtat 
      gtgaaaaac caaccattc aagctcact attatatta 
      aggttttcc acaacaaac gacgtgaaa atcgatgtt 
      tcaaaaaat atttccaaa gtgttaaga aagagaaca 
      aacccagcc actgcaacc ataaacctt gagaatttc 
      taccaagac tctgattta acagagttt tgtattttg 
      tcccaacct aaactcatg tcttacgtg ttacattta 
      tcccgtcta gcaaaaccc aaagccata atcttaagt 
      aataacgaa atcagaagt ttggttccg attgaggct 
      ctatgggga atcaatgtg acaagttta gatttgaga 
      aataatttg ataatgagt ttgaccgaa ctcgaacct 
      ttgacacaa cttaagatt actgagctt tggttggaa 
      ggcaatcct ctatgtgat aattacgat gagcacact 
      tatattcaa agagttaag gagtattgt ccaaaaatt 
      gaaagactt gacggtgtt ttattgaaa cacaacggc 
      tttccgtca tttagaaga aactttttg tgtaacagc 
      tgtggccat aatttagtt gaccaattt ctagattat 
      tacttcact tcctacgac tcttcaaat cgaggcatg 
      cttaaaaat ttataccac aagggtgcc ctattttcg 
      ttaactgct gactatcac tctacgcag ttgtcttca 
      gcttcagcc cagttgaag cgttacaag acgcactcc 
      agaaattta ctttcttta gcagactta tctcggagc 
      gatttgaat ttacagcaa ggaagtaaa gcgattata 
      gatttgtta aatttatta ccgccgtca gaacacgat 
      ccatattct ttcacggtc gatttaata cattattcg 
      gatgtttgt gccattgtc gttgtcact ggagtcttt 
      aaagaactg ccagagaca atcttggac gcggagcgg 
      tttatgggt tttaacagg tgttttgca cttgaatcc 
      ataaatgga gagtattgc attattaat gaacaaatg 
      catgtcttc aatgcgctc acttcgcag caagtgaag 
      gcattcaaa cacgagagg cccatcagg ccgcaaatg 
      ctcccacct gctcaaact gctaaacag aagagtcag 
      gttattaca gcaatgaaa ctcattaca aatttgacc 
      accgaatgg gccaaaaag tgtttagaa gaatgccac 
      tatgatttc aaaagcgca cttgcgctc tttgtagat 
      ctgtataaa gtggataaa attcccgcc gacggattt 
      caaaattaa
    mRNA sequence
    • >TC008131 RA
      gtggtggtc gtccttcgt gtttttgtt ttgtgtaca 
      aaaatatca atctgagct gtttaattc aattttaat 
      cagtttagt ttagataag aattggttt atttcacgt 
      aaaagcgtt ttgattaga agatatttc atataacct 
      aaaaatggc ttcaacgct gttttttgg tagcgagtc 
      ccagtcgat aacgaattt ggacgcgaa aaaaattcg 
      ccagagtcc gcacgctat tgtcgttaa cgatttttc 
      gatacagac tgggattct ctacaaaaa taaggaagt 
      tctggaaaa ccccatcta ctggcataa gtttattgt 
      gaataacgc cgaggattt tagccggga cttggtttt 
      gaaaacaat tctggacca tgtgcaacc gatggattt 
      aatcccggt tttcttcct caaagaagg cagttctgc 
      ttcgttcct tgcgcggaa ctgtggagc agcgattga 
      aaaaatatg taaagataa tttgattat tgataaccc 
      gtatgtgaa aaaccaacc attcaagct cactattat 
      attaaggtt ttccacaac aaacgacgt gaaaatcga 
      tgtttcaaa aaatatttc caaagtgtt aagaaagag 
      aacaaaccc agccactgc aaccataaa ccttgagaa 
      tttctacca agactctga tttaacaga gttttgtat 
      tttgtccca acctaaact catgtctta cgtgttaca 
      tttatcccg tctagcaaa acccaaagc cataatctt 
      aagtaataa cgaaatcag aagtttggt tccgattga 
      ggctctatg gggaatcaa tgtgacaag tttagattt 
      gagaaataa tttgataat gagtttgac cgaactcga 
      acctttgac acaacttaa gattactga gctttggtt 
      ggaaggcaa tcctctatg tgataatta cgatgagca 
      cacttatat tcaaagagt taaggagta ttgtccaaa 
      aattgaaag acttgacgg tgttttatt gaaacacaa 
      cggctttcc gtcatttag aagaaactt tttgtgtaa 
      cagctgtgg ccataattt agttgacca atttctaga 
      ttattactt cacttccta cgactcttc aaatcgagg 
      catgcttaa aaatttata ccacaaggg tgccctatt 
      ttcgttaac tgctgacta tcactctac gcagttgtc 
      ttcagcttc agcccagtt gaagcgtta caagacgca 
      ctccagaaa tttactttc tttagcaga cttatctcg 
      gagcgattt gaatttaca gcaaggaag taaagcgat 
      tatagattt gttaaattt attaccgcc gtcagaaca 
      cgatccata ttctttcac ggtcgattt aatacatta 
      ttcggatgt ttgtgccat tgtcgttgt cactggagt 
      ctttaaaga actgccaga gacaatctt ggacgcgga 
      gcggtttat gggttttaa caggtgttt tgcacttga 
      atccataaa tggagagta ttgcattat taatgaaca 
      aatgcatgt cttcaatgc gctcacttc gcagcaagt 
      gaaggcatt caaacacga gaggcccat caggccgca 
      aatgctccc acctgctca aactgctaa acagaagag 
      tcaggttat tacagcaat gaaactcat tacaaattt 
      gaccaccga atgggccaa aaagtgttt agaagaatg 
      ccactatga tttcaaaag cgcacttgc gctctttgt 
      agatctgta taaagtgga taaaattcc cgccgacgg 
      atttcaaaa ttaagcatc tcaaccaaa atattcatc 
      acaaataaa ggattttta aacta
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