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Gene information
General information
TC008122 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5326236..5329230
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008122
    iBeetle screen iB_04429

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva appendages rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008122 RA
      MTTQHTTQD TNSLSTRTA LISVFIIFV 
      SSLFALFLV YKTFPQVTE EERQHIKLP 
      WNIEDAKQL GIVLNSLQT FAIPGSLFL 
      SVLSGFLFP FTVALTLVC TCSAVGASL 
      CFLLSQLLG RKLVLKYFP EKAAAWGHQ 
      VNKHRDNLF NYVLFLRIT PFLPNWFIN 
      LTAPVIGVP LVPFALGTF FGVAPPSFI 
      AIQGGQTLQ NMTADDFKI SATSLVTLA 
      VCGIIPLLP IVFKGWLKK KIE
    • >TC008122 RB
      MIPNRQSIH EPNTNSLST RTALISVFI 
      IFVSSLFAL FLVYKTFPQ VTEEERQHI 
      KLPWNIEDA KQLGIVLNS LQTFAIPGS 
      LFLSVLSGF LFPFTVALT LVCTCSAVG 
      ASLCFLLSQ LLGRKLVLK YFPEKAAAW 
      GHQVNKHRD NLFNYVLFL RITPFLPNW 
      FINLTAPVI GVPLVPFAL GTFFGVAPP 
      SFIAIQGGQ TLQNMTADD FKISATSLV 
      TLAVCGIIP LLPIVFKGW LKKKIE
    CDS sequence
    • >TC008122 RA
      atgacgacg caacacacc actcaagat actaattct 
      ttatcaaca cggacggca ctaatttca gttttcatt 
      atttttgta agttcactc tttgcattg ttcttagtg 
      tacaaaacc tttcctcaa gtcactgag gaagaacga 
      caacacatt aaattgcca tggaacata gaagacgcc 
      aaacagcta ggaatagta ttaaatagt ctgcaaacg 
      tttgcaatc ccgggatct ctattcctc tcagtcctt 
      tcaggcttc ctattcccc ttcactgtg gccctgacc 
      ctagtgtgc acttgctca gccgtgggg gcctccctg 
      tgtttcctc ctgtctcag ttgctggga cgaaaacta 
      gtactcaag tatttcccc gaaaaagcg gcggcttgg 
      ggccaccaa gtgaacaaa caccgtgac aacttattc 
      aactacgta ttattttta agaataacc ccatttttg 
      cccaactgg tttataaat ttaaccgca cctgtgata 
      ggagtcccg ttggtgcct tttgcgcta ggaacgttt 
      ttcggagtg gcaccgcct tcgtttatc gccattcaa 
      ggcggacaa acgttgcaa aatatgacc gctgacgat 
      tttaaaata agtgctacg tctttggtt actttggcg 
      gtgtgtggg atcattccg ttgttacct attgtcttc 
      aaaggctgg ttgaagaag aaaatcgag tga
    • >TC008122 RB
      atgatcccg aaccgacaa tccattcat gaaccaaat 
      actaattct ttatcaaca cggacggca ctaatttca 
      gttttcatt atttttgta agttcactc tttgcattg 
      ttcttagtg tacaaaacc tttcctcaa gtcactgag 
      gaagaacga caacacatt aaattgcca tggaacata 
      gaagacgcc aaacagcta ggaatagta ttaaatagt 
      ctgcaaacg tttgcaatc ccgggatct ctattcctc 
      tcagtcctt tcaggcttc ctattcccc ttcactgtg 
      gccctgacc ctagtgtgc acttgctca gccgtgggg 
      gcctccctg tgtttcctc ctgtctcag ttgctggga 
      cgaaaacta gtactcaag tatttcccc gaaaaagcg 
      gcggcttgg ggccaccaa gtgaacaaa caccgtgac 
      aacttattc aactacgta ttattttta agaataacc 
      ccatttttg cccaactgg tttataaat ttaaccgca 
      cctgtgata ggagtcccg ttggtgcct tttgcgcta 
      ggaacgttt ttcggagtg gcaccgcct tcgtttatc 
      gccattcaa ggcggacaa acgttgcaa aatatgacc 
      gctgacgat tttaaaata agtgctacg tctttggtt 
      actttggcg gtgtgtggg atcattccg ttgttacct 
      attgtcttc aaaggctgg ttgaagaag aaaatcgag 
      tga
    mRNA sequence
    • >TC008122 RA
      taataaaca aatacgtat tgtacatac caataatta 
      aaccgatta aaatcaatt atgtatgtt cgtcccgtc 
      ttacgtaat attggtcgc gactggcaa gaaatgcaa 
      cttttcgcg tttaaaatt ggcaacaca tgcagatgg 
      cctggcggt ataaatagt tgaggagca cgtaattaa 
      tttgctggt acaaaagaa gcattagat gccgacgtc 
      tcgctctgg taaaaacac ctggtttgc cacaacaca 
      ggtgtttac aaaatttaa ttcagtgac agtgaaatt 
      gaatttatt cctggatga aactaattc gggaatcat 
      tcatgaaac gcgctagat ttgctaatt gcgaggaac 
      ggaccagtt agttgagct tgttttgtg gttttatta 
      acctgcgat atagttaga acggaatag tgcaacaac 
      cgaatatta caagttaca cgaatacag tttgctcca 
      aacaatttc aagtcttgc gtcaagaag aggtttcag 
      tgttttcga ggctttttt cattcgctt ggcacagat 
      tcaatgttg atgccctgt caaagtggc gataggctc 
      tcagccaat cacctcatg ccacagcat gtcagctga 
      tctcgttca agtcagtgc tgtcatctg tcaagttag 
      tttaaattt ccattaatt aaaaaaata aataacgta 
      aatatgaag cagtaaaac acaacaaac atatcgagt 
      tctacttta gataaattt cacgcaatt ccaaaccga 
      aaatgacga cgcaacaca ccactcaag atactaatt 
      ctttatcaa cacggacgg cactaattt cagttttca 
      ttatttttg taagttcac tctttgcat tgttcttag 
      tgtacaaaa cctttcctc aagtcactg aggaagaac 
      gacaacaca ttaaattgc catggaaca tagaagacg 
      ccaaacagc taggaatag tattaaata gtctgcaaa 
      cgtttgcaa tcccgggat ctctattcc tctcagtcc 
      tttcaggct tcctattcc ccttcactg tggccctga 
      ccctagtgt gcacttgct cagccgtgg gggcctccc 
      tgtgtttcc tcctgtctc agttgctgg gacgaaaac 
      tagtactca agtatttcc ccgaaaaag cggcggctt 
      ggggccacc aagtgaaca aacaccgtg acaacttat 
      tcaactacg tattatttt taagaataa ccccatttt 
      tgcccaact ggtttataa atttaaccg cacctgtga 
      taggagtcc cgttggtgc cttttgcgc taggaacgt 
      ttttcggag tggcaccgc cttcgttta tcgccattc 
      aaggcggac aaacgttgc aaaatatga ccgctgacg 
      attttaaaa taagtgcta cgtctttgg ttactttgg 
      cggtgtgtg ggatcattc cgttgttac ctattgtct 
      tcaaaggct ggttgaaga agaaaatcg agtgaaata 
      gtatttatt tttagttaa atcggttgt taaagaata 
      gcccattcc gttttgtga gttgtattt attttg
    • >TC008122 RB
      taataaaca aatacgtat tgtacatac caataatta 
      aaccgatta aaatcaatt atgtatgtt cgtcccgtc 
      ttacgtaat attggtcgc gactggcaa gaaatgcaa 
      cttttcgcg tttaaaatt ggcaacaca tgcagatgg 
      cctggcggt ataaatagt tgaggagca cgtaattaa 
      tttgctggt acaaaagaa gcattagat gccgacgtc 
      tcgctctgg taaaaacac ctggtttgc cacaacaca 
      ggtgtttac aaaatttaa ttcagtgac agtgaaatt 
      gaatttatt cctggatga aactaattc gggaatcat 
      tcatgaaac gcgctagat ttgctaatt gcgaggaac 
      ggaccagtt agttgagct tgttttgtg gttttatta 
      acctgcgat atagttaga acggaatag tgcaacaac 
      cgaatatta caagttaca cgaatacag tttgctcca 
      aacaatttc aagtcttgc gtcaagaag aggtttcag 
      tgttttcga ggctttttt cattcgctt ggcacagat 
      tcaatgttg atgccctgt caaagtggc gataggctc 
      tcagccaat cacctcatg ccacagcat gtcagctga 
      tctcgttca agtcagtgc tgtcatctg tcaagttag 
      tttaaattt ccattaatt aaaaaaata aataacgta 
      aatatgaag cagtaaaac acaacaaac atatcgagt 
      tctacttta gataaattt cacgcaatt ccaaaccga 
      aaatgacga cgcaacaca ccactcaag gtgacagtc 
      aaattcaat gttttcaaa tatcatcca cctcagaca 
      tttacaatc gccctttcg tatccccat tccaactca 
      ggggtagac ctacatgcc gtagatgca ttcatttta 
      ccaatcatt ctccaatta tgatcccga accgacaat 
      ccattcatg aaccaaata ctaattctt tatcaacac 
      ggacggcac taatttcag ttttcatta tttttgtaa 
      gttcactct ttgcattgt tcttagtgt acaaaacct 
      ttcctcaag tcactgagg aagaacgac aacacatta 
      aattgccat ggaacatag aagacgcca aacagctag 
      gaatagtat taaatagtc tgcaaacgt ttgcaatcc 
      cgggatctc tattcctct cagtccttt caggcttcc 
      tattcccct tcactgtgg ccctgaccc tagtgtgca 
      cttgctcag ccgtggggg cctccctgt gtttcctcc 
      tgtctcagt tgctgggac gaaaactag tactcaagt 
      atttccccg aaaaagcgg cggcttggg gccaccaag 
      tgaacaaac accgtgaca acttattca actacgtat 
      tatttttaa gaataaccc catttttgc ccaactggt 
      ttataaatt taaccgcac ctgtgatag gagtcccgt 
      tggtgcctt ttgcgctag gaacgtttt tcggagtgg 
      caccgcctt cgtttatcg ccattcaag gcggacaaa 
      cgttgcaaa atatgaccg ctgacgatt ttaaaataa 
      gtgctacgt ctttggtta ctttggcgg tgtgtggga 
      tcattccgt tgttaccta ttgtcttca aaggctggt 
      tgaagaaga aaatcgagt gaaatagta tttattttt 
      agttaaatc ggttgttaa agaatagcc cattccgtt 
      ttgtgagtt gtatttatt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY