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Gene information
General information
TC008101 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5026873..5028587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008101
    iBeetle screen iB_04425

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008101 RA
      MSVEQLQNI ISPKINQQV LTDIVVKKS GLKDIQIKE 
      IKIEGACTR KGDSYLSQI CRFTIEATG KNKSNQENE 
      TSIPVIAKF LPKNLARRK TYRSPVFFE NEGIFYETV 
      LPAFQDFQN SKKLSKKFE NIPLMLAQW IDGDNDFII 
      LEDVTPQGY KPAVRGSDL DENHSVAIL KLLANFHSL 
      SIAFRDQKP EIFEKAAAS LKETYFAEK YRSWFEPFL 
      HKLYIVALD AVENEFPQL LPKLKKYIS GDVFGKICQ 
      GYQLRGPYA VVAHGDVWA PNFLIKYAE KDRIEDIVM 
      IDFQLTRYA TLAGDLIFY LLTCVDINL IEQKWNFLI 
      KEYHVTLTE NLAQLGSDP NLINLEGLH RELKESMKF 
      GLGVVIESL IMSLLDETE VGDLDGIQG DEAVSVENV 
      WKVSPFKTQ AKRLRFARM FKFLVDNNF I
    CDS sequence
    • >TC008101 RA
      atgtcggtc gaacagctt caaaatatt atttcgccg 
      aaaattaat cagcaagtc cttacggac attgtcgtg 
      aaaaaaagt ggtttgaag gatattcaa ataaaagaa 
      attaaaata gaaggggcc tgcacgaga aaaggggat 
      tcgtatttg agccaaatt tgccgattt acaattgag 
      gcaacggga aaaaataaa tcaaatcag gaaaatgaa 
      acgtcaatc cccgtaatt gcaaaattc ctaccgaaa 
      aatctcgct cgtcgcaaa acctaccgc tcccctgta 
      tttttcgaa aatgaggga atattctac gaaacagtt 
      ttgccggcc ttccaagac ttccaaaac tcgaaaaag 
      ttgtccaaa aaattcgaa aacattcct ttaatgctt 
      gcacaatgg attgacggc gataacgat ttcataatt 
      ctcgaagac gtgactccc caaggatac aagcctgct 
      gttcgaggc tcagactta gatgaaaac cactctgtg 
      gctatttta aaattactt gcaaatttt cattctctg 
      agtatcgct ttcagggac caaaagcca gaaatattc 
      gaaaaagcc gcggcgagt ttaaaagag acatacttt 
      gccgaaaaa taccgaagc tggttcgaa cctttcctc 
      cacaaactc tatattgtg gccttagac gcagtcgag 
      aatgaattt ccccaactt ttaccaaaa ttaaaaaaa 
      tacatttct ggcgatgtc ttcggtaaa atttgtcag 
      ggttaccaa ctccgcggc ccctacgcc gttgtagca 
      cacggcgac gtttgggcc ccaaatttt ttaataaaa 
      tatgcggaa aaagatcga attgaagat atcgtaatg 
      atcgatttt caattaaca cgatacgca actctcgct 
      ggcgattta attttctat ttattgact tgtgtcgac 
      attaatctg atcgaacaa aagtggaat tttttgatc 
      aaagaatat cacgtgact ttgaccgaa aatttggcg 
      caattgggg tccgacccg aatttaatt aatttagaa 
      gggttgcac agggaattg aaagaaagt atgaagttt 
      ggcctaggt gtcgtaata gagtcctta attatgtca 
      cttctggat gaaaccgaa gttggagat cttgatgga 
      attcaggga gacgaagca gtatccgtg gaaaacgta 
      tggaaagtg tctcccttt aaaacccaa gccaagcgg 
      ctcagattc gcccgaatg ttcaaattt ttggtcgat 
      aacaatttt atctaa
    mRNA sequence
    • >TC008101 RA
      ctataaaag tgttctagg gttgaaatt attgttctt 
      tcactgacg tggctttag tgcgcttga caatgtcgg 
      tcgaacagc ttcaaaata ttatttcgc cgaaaatta 
      atcagcaag tccttacgg acattgtcg tgaaaaaaa 
      gtggtttga aggatattc aaataaaag aaattaaaa 
      tagaagggg cctgcacga gaaaagggg attcgtatt 
      tgagccaaa tttgccgat ttacaattg aggcaacgg 
      gaaaaaata aatcaaatc aggaaaatg aaacgtcaa 
      tccccgtaa ttgcaaaat tcctaccga aaaatctcg 
      ctcgtcgca aaacctacc gctcccctg tatttttcg 
      aaaatgagg gaatattct acgaaacag ttttgccgg 
      ccttccaag acttccaaa actcgaaaa agttgtcca 
      aaaaattcg aaaacattc ctttaatgc ttgcacaat 
      ggattgacg gcgataacg atttcataa ttctcgaag 
      acgtgactc cccaaggat acaagcctg ctgttcgag 
      gctcagact tagatgaaa accactctg tggctattt 
      taaaattac ttgcaaatt ttcattctc tgagtatcg 
      ctttcaggg accaaaagc cagaaatat tcgaaaaag 
      ccgcggcga gtttaaaag agacatact ttgccgaaa 
      aataccgaa gctggttcg aacctttcc tccacaaac 
      tctatattg tggccttag acgcagtcg agaatgaat 
      ttccccaac ttttaccaa aattaaaaa aatacattt 
      ctggcgatg tcttcggta aaatttgtc agggttacc 
      aactccgcg gcccctacg ccgttgtag cacacggcg 
      acgtttggg ccccaaatt ttttaataa aatatgcgg 
      aaaaagatc gaattgaag atatcgtaa tgatcgatt 
      ttcaattaa cacgatacg caactctcg ctggcgatt 
      taattttct atttattga cttgtgtcg acattaatc 
      tgatcgaac aaaagtgga attttttga tcaaagaat 
      atcacgtga ctttgaccg aaaatttgg cgcaattgg 
      ggtccgacc cgaatttaa ttaatttag aagggttgc 
      acagggaat tgaaagaaa gtatgaagt ttggcctag 
      gtgtcgtaa tagagtcct taattatgt cacttctgg 
      atgaaaccg aagttggag atcttgatg gaattcagg 
      gagacgaag cagtatccg tggaaaacg tatggaaag 
      tgtctccct ttaaaaccc aagccaagc ggctcagat 
      tcgcccgaa tgttcaaat ttttggtcg ataacaatt 
      ttatctaag tcttgtcca aataattta gttttatca 
      aacaataca taagagtaa acaattaca ctctcctga 
      taagtaatt tcctgcaat tatctaatc aaaacaaat 
      aaaaatttg aaaaatgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY