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Gene information
General information
TC008090 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4952413..4959397
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008090
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04423

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    labium orientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    maxilla orientation irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    metathorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    mesothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008090 RA
      MWGRAQLLV LLTALSAYG DDVHPEDSQ LRVGRSVDI 
      FTRYGYLSL SMKVVPRND SDFLFREPT IDVFDDLDR 
      YVVHPVKVP KKQRRVFVG DFHMEFCDN VKQLVQAYF 
      RDFSFERLE NTWRAFTAS WSPDTLAKN LGINSSFVH 
      KEHCYVLVR LARFRDSVK LSKIPNNVN LMDVVAREI 
      DKIQVGDVS SVLKFIRKY GSHFIESYV TGNALYQVF 
      VFRRSIYLQ IKERLKTQG ISHLDKLEL GQYFSPWFA 
      EHMGQIKVA SGNHTVEEW AAAKLRVHY YIFTYPTLL 
      KIHGDTQLL TVLNDLLQN EAILQMGMK TLAPAFKDP 
      AKRTFFIEV LDNFLKLWE SNL
    CDS sequence
    • >TC008090 RA
      atgtggggc cgagcgcag ctgctcgtg ctgctgacc 
      gcgctctcg gcctacgga gacgacgtc catccggag 
      gacagccag ctgcgggtc ggccgctcc gtggacatc 
      ttcacccgc tacggctac ctcagtctg agcatgaaa 
      gtggtgccg cggaacgac tccgacttc ctcttcaga 
      gagcccacc atcgacgtc ttcgacgac ctggaccgg 
      tacgtggtg cacccggtc aaggtgccg aagaagcag 
      cgaagggtc ttcgtcggc gacttccat atggagttc 
      tgcgataac gtgaaacag ctagtgcag gcctatttc 
      agggatttc agcttcgag aggctggag aatacgtgg 
      agggcgttc acggcgagc tggtcgccg gacacgctg 
      gccaagaat ttagggatt aattcgtcg tttgttcac 
      aaggaacat tgttatgtg ctagtgagg ttggcgagg 
      tttagggat agtgtcaag ctgagcaaa ataccgaat 
      aatgttaat ttgatggac gtggtggcg agggagatt 
      gataaaata caagttggt gatgtgagc agtgtgctg 
      aagtttata cgaaagtac ggctcgcat tttatcgag 
      tcgtacgtt actggaaac gcactttac caggtgttc 
      gtcttccgg cgctccatc tacttacaa atcaaagag 
      cggctcaaa acgcagggc atctcccac ttggacaag 
      ctggagctt ggccagtac ttcagcccc tggtttgcc 
      gaacacatg gggcagata aaggtggcc agcggcaac 
      cacaccgtg gaggaatgg gccgctgcc aagctgcgc 
      gtccattac tacattttc acatatcca acccttctg 
      aagatccac ggcgacacc caactgctc accgtcctc 
      aacgacctc ctccagaac gaggccatc ctccaaatg 
      ggcatgaaa accctagcg ccggcgttc aaggacccg 
      gccaaacgc acgttcttc atcgaagtg ttggacaat 
      tttttgaaa ttgtgggag agcaatttg tag
    mRNA sequence
    • >TC008090 RA
      tttcaaaat caaaatcgc aaatgacca tcaccaatg 
      aagccgaaa cgttgcggt gtcagtcgc gtcgagaat 
      gtggggccg agcgcagct gctcgtgct gctgaccgc 
      gctctcggc ctacggaga cgacgtcca tccggagga 
      cagccagct gcgggtcgg ccgctccgt ggacatctt 
      cacccgcta cggctacct cagtctgag catgaaagt 
      ggtgccgcg gaacgactc cgacttcct cttcagaga 
      gcccaccat cgacgtctt cgacgacct ggaccggta 
      cgtggtgca cccggtcaa ggtgccgaa gaagcagcg 
      aagggtctt cgtcggcga cttccatat ggagttctg 
      cgataacgt gaaacagct agtgcaggc ctatttcag 
      ggatttcag cttcgagag gctggagaa tacgtggag 
      ggcgttcac ggcgagctg gtcgccgga cacgctggc 
      caagaattt agggattaa ttcgtcgtt tgttcacaa 
      ggaacattg ttatgtgct agtgaggtt ggcgaggtt 
      tagggatag tgtcaagct gagcaaaat accgaataa 
      tgttaattt gatggacgt ggtggcgag ggagattga 
      taaaataca agttggtga tgtgagcag tgtgctgaa 
      gtttatacg aaagtacgg ctcgcattt tatcgagtc 
      gtacgttac tggaaacgc actttacca ggtgttcgt 
      cttccggcg ctccatcta cttacaaat caaagagcg 
      gctcaaaac gcagggcat ctcccactt ggacaagct 
      ggagcttgg ccagtactt cagcccctg gtttgccga 
      acacatggg gcagataaa ggtggccag cggcaacca 
      caccgtgga ggaatgggc cgctgccaa gctgcgcgt 
      ccattacta cattttcac atatccaac ccttctgaa 
      gatccacgg cgacaccca actgctcac cgtcctcaa 
      cgacctcct ccagaacga ggccatcct ccaaatggg 
      catgaaaac cctagcgcc ggcgttcaa ggacccggc 
      caaacgcac gttcttcat cgaagtgtt ggacaattt 
      tttgaaatt gtgggagag caatttgta gtgcatcct 
      gttttttgt gataatacg ttgtgataa ggacgcccg 
      cggagagga aggactttt gcattgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY