Status
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Gene information
General information
TC008073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4649682..4654718
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008073
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04422

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008073 RA
      MRVRWNTYG INLLRETDI KRKIELTQI KRQIEMLST 
      FMPDEILNM PLSELDEIH NFVGVLLFA DVSGFTPLC 
      EKYYKTGKG GIYRLTATL NAYIGALVE VITFYGGDI 
      LKFSGDAFL ALWKAPPDL RLFEVIHQV IVCALFIQN 
      TLGCFETEV NVLLKVKLA IACGNLTFS VIGNESYRH 
      YIITGQAIN DVKCAERES VSGDVVVAP SAWGHLAED 
      NYEVTYAPD GNVKIWNCV YFPNQAALK QHYANKSPV 
      AHALCVKHL EHRSQLHEK RRIDDATTD SKITESFIA 
      SLPQRPSVK AAPMNWIPN DLNPFIVRP VQSQVEEKQ 
      PIEYLTEMR EVTIQFINI IPTTFQDSK LVPMVNTAY 
      QIVCKIVSK VRGVVNKVS LFDKDCMIL VLFGLRGIE 
      HEVESQNAL KSAFAIRKA IKKLTTVKS ISIGVTNGF 
      VYCGVVGHP FRREYTVIG GAVNKAARI MCAYEGKVT 
      CDHETYKSC KLSSLYFQV QSALKLKGI TDAGYIFEY 
      NEDFDKMAY EDKQSMPLI GRRMEMGVA DVILQHPEL 
      AGWTSICFK GESKVGKTK ILQEINKRY ATSNRYICS 
      MVLNEGLQR PYYTASTIY HQLYDIMSK HNPAVLEQL 
      PRYLWDLNE ILQVNRSTT SIDMLSTHS KELIIEHFI 
      KMCQGLRPI LVFIDNIQF IDRRSLDLI EQVLQRKLI 
      SLVCAGCFE DNTTWNTMW KLSRSNQIK VFNIEPLPS 
      SEIALLMCS FLNVKGISK KLVVLINKT CEGRPGYVQ 
      ACLLKMINN GELEVKYAL TSETEYAFL KDADQKHLV 
      LVAGFSKKY LEHGEETST AVTLDLFDS FSPYQQLII 
      KTAAVLGDI FTRTTLIVL LKYPNESML AAAIKTLFE 
      EEIFDCASR YVNSGGLQE KKNICLCYM EEEEYDLDL 
      ELPRYAFCK VLHFKSRNI KTLAYELLP LNQKKELHL 
      RITDLLENQ NNSCPKCLR DNSASIISV RRFKDLIKY 
      CHDQKYIWS EKYNENYVS VEDYKGIIR EYVHDPPVY 
      APNSKKRVW RSSDVPKRR VWDPTTCFC LEILTKVYR 
      DLIHHSHCA QHLGKKIFF MLEYGVILS IMSEYEESL 
      PVLHEASEL CMVQTNKAY VFTGNFKQV MMGKIHYLM 
      GHIYLKMGN TTAAKNHAL LSLRQYDVP LISFKYTLP 
      YMLLNKAWF LRFGRVFDN ITKQDNPLI KLDFGLCMG 
      LIGNIYAAE GCWQLAKQA AAKSVLCLR RTNSNIDVL 
      CDVYTSAVY LYSFCGDMH ICEKLERCF NKEVLKYYS 
      NNITHELRA ISTVIGVLF LVKVLSGAI IESLQLGYR 
      TVHLQLSLE AHYSLIEVV PTLATVLIC LERVAEAVH 
      MARILLHFG QKYHTHSYI GYYAFCVEL LLETNFCLE 
      PIEKIEQFA HHLIDQKKY HSDFGNKLI INIYCYWLR 
      YKPALAPSW KKLYNVTES DNFKIMSIL NTMRYTECL 
      LLEMVRTLE RTEPILQAQ IDEANTFLN RMKQAAKKW 
      PFFRPRFWH LKAYNARIF NKLQATIKF LKTANSFAA 
      EDGNILEEC WTMLHATSW FGGFSSDKE PKHLKWELA 
      KTYTPLQWC QILYALPAE
    CDS sequence
    • >TC008073 RA
      atgcgcgta agatggaac acctacggc ataaacctc 
      ctccgggag accgacatc aagcgcaaa atcgaatta 
      acccaaatc aagcgccag atcgaaatg ctttcgact 
      ttcatgccc gacgaaatc ctcaacatg cctctgtcg 
      gaactagac gagatccac aacttcgtc ggagtgctc 
      ctgttcgca gacgtctcc gggttcact cccttgtgt 
      gaaaagtac tacaagacg ggaaaaggc ggcatctac 
      cgactcaca gcaactctg aacgcttac atcggagct 
      ttagttgaa gttataacg ttctatgga ggcgatatt 
      ttgaaattt tcgggtgat gccttcctt gcactatgg 
      aaggcccca cccgactta agactcttc gaagtcatc 
      caccaggtg atagtgtgc gccttattc atccaaaac 
      acacttggc tgcttcgaa accgaagtc aatgtctta 
      ctcaaagta aaactcgcc atagcttgt ggcaattta 
      actttctcg gtgatcggt aacgagtct tacagacat 
      tacattata actggtcag gcaatcaat gacgtgaag 
      tgtgcagaa cgcgagagt gtctctggt gatgttgtt 
      gtggcacca agtgcttgg ggacacctc gctgaggac 
      aactacgag gttacttat gcccctgat ggcaatgtt 
      aagatttgg aactgtgtt tattttccc aaccaagcg 
      gctttgaag caacactac gccaacaaa agtccagta 
      gcgcacgca ctctgtgtc aaacatttg gaacacaga 
      agccagctg catgagaag agacgaatc gacgatgcg 
      acaactgat agtaaaatc acggagagt tttattgca 
      agtttgcca caaagacca agcgttaag gcggctcca 
      atgaactgg attccgaac gatctgaac ccgtttatc 
      gtccggccg gtccagagc caagtcgag gaaaaacaa 
      ccaattgag tatctcacc gaaatgcgc gaagtcaca 
      atccagttc attaatatc atccccacc actttccaa 
      gacagtaaa ctggttcca atggtcaac accgcctac 
      caaattgtg tgcaaaatc gtgtctaaa gtccgagga 
      gtcgtcaat aaagtgtct ctttttgat aaagactgt 
      atgattttg gttctgttt ggtctgaga ggcattgaa 
      cacgaagtt gaaagtcag aacgcactc aagtcggct 
      ttcgccatc cgaaaagcc atcaagaaa cttactacc 
      gttaagtca atttcaatc ggagtcact aacggtttt 
      gtctattgt ggagtcgtt ggccaccct ttccggcga 
      gaatacacg gtcattgga ggtgcagtt aacaaagca 
      gcgcggata atgtgtgcc tacgaaggc aaggtcact 
      tgtgaccac gagacttac aagagttgc aaactctcg 
      tcactctat ttccaagtg cagtcagcg cttaaattg 
      aaaggtata acagatgct ggctacatt tttgaatat 
      aatgaagat ttcgataaa atggcctat gaggacaaa 
      caatcgatg ccgctaatt ggccgtcgt atggaaatg 
      ggagtggct gatgttatt ttacaacac ccggagtta 
      gccggttgg actagtatt tgtttcaag ggggagtcc 
      aaagttggt aaaacgaaa atcttgcaa gagatcaat 
      aagagatat gcgacttca aaccgatat atttgttct 
      atggtcttg aacgaggga ctccaaagg ccttactat 
      accgcttca acgatttac caccaacta tacgacatt 
      atgtcaaaa cacaatcct gccgttttg gagcagttg 
      cctcgctac ttgtgggat ttgaacgaa atcctccaa 
      gtcaatcga tcaacgact tcgatcgac atgctgagt 
      actcacagt aaagaattg atcattgaa cactttatc 
      aaaatgtgt caaggatta cgcccgatt ttagtgttt 
      attgacaac atccagttt attgatagg cgaagtctg 
      gacttgatt gagcaagtc cttcagaga aaactcatc 
      agtctggta tgtgccggt tgtttcgaa gacaacacg 
      acttggaac accatgtgg aagttgtcc cgaagcaac 
      cagatcaaa gttttcaac attgagccg ttaccatcg 
      agcgaaatt gccctcctg atgtgtagt tttctcaat 
      gtgaaaggc atatcaaaa aaactggta gtactgata 
      aataaaact tgcgaaggc cggcctggc tatgtccaa 
      gcctgtttg ctcaaaatg atcaataat ggcgaacta 
      gaggttaag tatgcccta accagtgaa actgaatac 
      gcgtttttg aaagatgct gaccagaag catctagtc 
      ctagtggct gggttttca aagaagtac ctcgagcat 
      ggcgaggag acttcgact gcggtcacg cttgatttg 
      tttgattcg ttctcgcct tatcaacag ctgataatt 
      aagacggcg gctgtcctg ggtgatatt tttactcgt 
      acgacttta atcgtcctt ttgaaatat ccaaatgag 
      agtatgttg gctgcggct attaagact ttgtttgag 
      gaagaaatc ttcgattgt gcttcacgt tacgtgaac 
      tcgggtggt ttgcaagag aagaaaaac atttgtttg 
      tgctacatg gaagaggag gaatatgac ttggaccta 
      gagttgcca agatacgcg ttttgtaaa gttttgcac 
      ttcaagagt cgcaacatc aagactttg gcctacgaa 
      ctgttgcct ctcaaccaa aagaaggaa ctccacttg 
      agaatcacc gatttgtta gaaaaccaa aacaattcc 
      tgtcccaaa tgtctccgc gacaattca gcatcgata 
      atctcagtc cgacgtttc aaagacttg atcaagtat 
      tgccacgac caaaagtac atctggtcg gagaaatac 
      aacgagaat tacgtttca gtcgaggat tacaaggga 
      attatcaga gaatacgtc catgatccg cctgtttat 
      gcacccaat tcaaagaaa cgtgtgtgg agaagttcc 
      gatgttccc aaacgccga gtttgggac cccaccacg 
      tgtttctgt ctggaaatc ttgactaaa gtttaccgc 
      gacttgatc catcacagt cattgtgcc caacatttg 
      ggcaagaaa atattcttc atgctagag tacggcgtg 
      attttatcg atcatgtcc gagtatgag gagtctctc 
      ccagtgtta cacgaagca agcgagctt tgcatggta 
      caaacgaac aaggcttac gtcttcacc ggaaacttc 
      aaacaagtc atgatggga aaaatccat tatttgatg 
      gggcacatt tacttgaaa atgggcaac acaacggcg 
      gccaagaac catgccttg ctcagtctg cggcaatat 
      gacgttcct ttgatttcg ttcaagtat acgttgcct 
      tacatgttg ttgaacaag gcgtggttt ctcagattc 
      ggccgtgtt tttgataat atcaccaag caggataat 
      ccgctgata aagctagac tttggactg tgcatgggt 
      ttgattggg aacatttac gccgctgag ggctgctgg 
      caattggcc aaacaagcc gctgcgaag agcgtcctg 
      tgtctaaga cgtaccaac tcaaatatt gacgtcttg 
      tgtgatgtg tacaccagt gccgtttat ttgtacagc 
      ttttgcggc gacatgcac atctgtgag aagctcgag 
      cgttgcttc aacaaggag gtactaaag tactactcc 
      aacaacata acgcacgaa cttcgcgct atttcgaca 
      gtgattgga gtgttgttt ttggtcaaa gttttgagt 
      ggtgccatc attgaatcc ctccaattg gggtaccgt 
      acggtacac ttgcaatta tcgcttgaa gcccactat 
      tccttgatc gaggtggta ccgacactt gccaccgtt 
      ctgatttgt ctagaacga gtcgccgag gccgtgcac 
      atggccaga attttacta cacttcggc caaaagtac 
      cacactcac agctacatc ggttattac gcattttgt 
      gtcgagctg ctcctcgaa accaatttc tgcctcgaa 
      cccatcgag aaaatcgag cagtttgcg caccatttg 
      atcgaccaa aagaaatac cactcggat tttggcaac 
      aaactcatt attaatatt tattgttat tggttgagg 
      tacaagccg gcgcttgca ccctcttgg aaaaaacta 
      tacaatgtg actgaatcc gacaatttc aaaataatg 
      agtattttg aatacgatg cgatatact gagtgtctt 
      ctactcgaa atggttcga acgttagaa cgcacggaa 
      ccgatcctc caagcccag atcgacgaa gccaacact 
      ttcttgaac agaatgaag caagcggcg aagaagtgg 
      ccgttcttc aggccgaga ttttggcat ttgaaggcg 
      tataatgca aggattttt aataagttg caagcgact 
      attaagttt ctgaagact gctaattcg tttgcggcc 
      gaggatggg aatattttg gaggagtgc tggactatg 
      ttgcatgca acgtcttgg ttcggaggg ttttcgtcg 
      gataaagag cccaaacat cttaaatgg gagttggca 
      aagacctac acgccgttg cagtggtgt cagattttg 
      tacgcccta ccggctgaa tag
    mRNA sequence
    • >TC008073 RA
      gacttgcgc ttagagtgc cattatttg ccgacatcc 
      gccgtgtgt atttaacaa aacaaatca ctatcgagt 
      tgttaaact tcgtaaatc gctattggc ggagcaact 
      tcatttcgc gcgcaaagg gagaattac gacacggaa 
      aatcgttaa taaaacaca atcgactaa ataataaca 
      aatcaagtg agtgaccct catttaaaa attgtcact 
      cgagttgtt ccattttgg ccaaaatgc gcgtaagat 
      ggaacacct acggcataa acctcctcc gggagaccg 
      acatcaagc gcaaaatcg aattaaccc aaatcaagc 
      gccagatcg aaatgcttt cgactttca tgcccgacg 
      aaatcctca acatgcctc tgtcggaac tagacgaga 
      tccacaact tcgtcggag tgctcctgt tcgcagacg 
      tctccgggt tcactccct tgtgtgaaa agtactaca 
      agacgggaa aaggcggca tctaccgac tcacagcaa 
      ctctgaacg cttacatcg gagctttag ttgaagtta 
      taacgttct atggaggcg atattttga aattttcgg 
      gtgatgcct tccttgcac tatggaagg ccccacccg 
      acttaagac tcttcgaag tcatccacc aggtgatag 
      tgtgcgcct tattcatcc aaaacacac ttggctgct 
      tcgaaaccg aagtcaatg tcttactca aagtaaaac 
      tcgccatag cttgtggca atttaactt tctcggtga 
      tcggtaacg agtcttaca gacattaca ttataactg 
      gtcaggcaa tcaatgacg tgaagtgtg cagaacgcg 
      agagtgtct ctggtgatg ttgttgtgg caccaagtg 
      cttggggac acctcgctg aggacaact acgaggtta 
      cttatgccc ctgatggca atgttaaga tttggaact 
      gtgtttatt ttcccaacc aagcggctt tgaagcaac 
      actacgcca acaaaagtc cagtagcgc acgcactct 
      gtgtcaaac atttggaac acagaagcc agctgcatg 
      agaagagac gaatcgacg atgcgacaa ctgatagta 
      aaatcacgg agagtttta ttgcaagtt tgccacaaa 
      gaccaagcg ttaaggcgg ctccaatga actggattc 
      cgaacgatc tgaacccgt ttatcgtcc ggccggtcc 
      agagccaag tcgaggaaa aacaaccaa ttgagtatc 
      tcaccgaaa tgcgcgaag tcacaatcc agttcatta 
      atatcatcc ccaccactt tccaagaca gtaaactgg 
      ttccaatgg tcaacaccg cctaccaaa ttgtgtgca 
      aaatcgtgt ctaaagtcc gaggagtcg tcaataaag 
      tgtctcttt ttgataaag actgtatga ttttggttc 
      tgtttggtc tgagaggca ttgaacacg aagttgaaa 
      gtcagaacg cactcaagt cggctttcg ccatccgaa 
      aagccatca agaaactta ctaccgtta agtcaattt 
      caatcggag tcactaacg gttttgtct attgtggag 
      tcgttggcc accctttcc ggcgagaat acacggtca 
      ttggaggtg cagttaaca aagcagcgc ggataatgt 
      gtgcctacg aaggcaagg tcacttgtg accacgaga 
      cttacaaga gttgcaaac tctcgtcac tctatttcc 
      aagtgcagt cagcgctta aattgaaag gtataacag 
      atgctggct acatttttg aatataatg aagatttcg 
      ataaaatgg cctatgagg acaaacaat cgatgccgc 
      taattggcc gtcgtatgg aaatgggag tggctgatg 
      ttattttac aacacccgg agttagccg gttggacta 
      gtatttgtt tcaaggggg agtccaaag ttggtaaaa 
      cgaaaatct tgcaagaga tcaataaga gatatgcga 
      cttcaaacc gatatattt gttctatgg tcttgaacg 
      agggactcc aaaggcctt actataccg cttcaacga 
      tttaccacc aactatacg acattatgt caaaacaca 
      atcctgccg ttttggagc agttgcctc gctacttgt 
      gggatttga acgaaatcc tccaagtca atcgatcaa 
      cgacttcga tcgacatgc tgagtactc acagtaaag 
      aattgatca ttgaacact ttatcaaaa tgtgtcaag 
      gattacgcc cgattttag tgtttattg acaacatcc 
      agtttattg ataggcgaa gtctggact tgattgagc 
      aagtccttc agagaaaac tcatcagtc tggtatgtg 
      ccggttgtt tcgaagaca acacgactt ggaacacca 
      tgtggaagt tgtcccgaa gcaaccaga tcaaagttt 
      tcaacattg agccgttac catcgagcg aaattgccc 
      tcctgatgt gtagttttc tcaatgtga aaggcatat 
      caaaaaaac tggtagtac tgataaata aaacttgcg 
      aaggccggc ctggctatg tccaagcct gtttgctca 
      aaatgatca ataatggcg aactagagg ttaagtatg 
      ccctaacca gtgaaactg aatacgcgt ttttgaaag 
      atgctgacc agaagcatc tagtcctag tggctgggt 
      tttcaaaga agtacctcg agcatggcg aggagactt 
      cgactgcgg tcacgcttg atttgtttg attcgttct 
      cgccttatc aacagctga taattaaga cggcggctg 
      tcctgggtg atattttta ctcgtacga ctttaatcg 
      tccttttga aatatccaa atgagagta tgttggctg 
      cggctatta agactttgt ttgaggaag aaatcttcg 
      attgtgctt cacgttacg tgaactcgg gtggtttgc 
      aagagaaga aaaacattt gtttgtgct acatggaag 
      aggaggaat atgacttgg acctagagt tgccaagat 
      acgcgtttt gtaaagttt tgcacttca agagtcgca 
      acatcaaga ctttggcct acgaactgt tgcctctca 
      accaaaaga aggaactcc acttgagaa tcaccgatt 
      tgttagaaa accaaaaca attcctgtc ccaaatgtc 
      tccgcgaca attcagcat cgataatct cagtccgac 
      gtttcaaag acttgatca agtattgcc acgaccaaa 
      agtacatct ggtcggaga aatacaacg agaattacg 
      tttcagtcg aggattaca agggaatta tcagagaat 
      acgtccatg atccgcctg tttatgcac ccaattcaa 
      agaaacgtg tgtggagaa gttccgatg ttcccaaac 
      gccgagttt gggacccca ccacgtgtt tctgtctgg 
      aaatcttga ctaaagttt accgcgact tgatccatc 
      acagtcatt gtgcccaac atttgggca agaaaatat 
      tcttcatgc tagagtacg gcgtgattt tatcgatca 
      tgtccgagt atgaggagt ctctcccag tgttacacg 
      aagcaagcg agctttgca tggtacaaa cgaacaagg 
      cttacgtct tcaccggaa acttcaaac aagtcatga 
      tgggaaaaa tccattatt tgatggggc acatttact 
      tgaaaatgg gcaacacaa cggcggcca agaaccatg 
      ccttgctca gtctgcggc aatatgacg ttcctttga 
      tttcgttca agtatacgt tgccttaca tgttgttga 
      acaaggcgt ggtttctca gattcggcc gtgtttttg 
      ataatatca ccaagcagg ataatccgc tgataaagc 
      tagactttg gactgtgca tgggtttga ttgggaaca 
      tttacgccg ctgagggct gctggcaat tggccaaac 
      aagccgctg cgaagagcg tcctgtgtc taagacgta 
      ccaactcaa atattgacg tcttgtgtg atgtgtaca 
      ccagtgccg tttatttgt acagctttt gcggcgaca 
      tgcacatct gtgagaagc tcgagcgtt gcttcaaca 
      aggaggtac taaagtact actccaaca acataacgc 
      acgaacttc gcgctattt cgacagtga ttggagtgt 
      tgtttttgg tcaaagttt tgagtggtg ccatcattg 
      aatccctcc aattggggt accgtacgg tacacttgc 
      aattatcgc ttgaagccc actattcct tgatcgagg 
      tggtaccga cacttgcca ccgttctga tttgtctag 
      aacgagtcg ccgaggccg tgcacatgg ccagaattt 
      tactacact tcggccaaa agtaccaca ctcacagct 
      acatcggtt attacgcat tttgtgtcg agctgctcc 
      tcgaaacca atttctgcc tcgaaccca tcgagaaaa 
      tcgagcagt ttgcgcacc atttgatcg accaaaaga 
      aataccact cggattttg gcaacaaac tcattatta 
      atatttatt gttattggt tgaggtaca agccggcgc 
      ttgcaccct cttggaaaa aactataca atgtgactg 
      aatccgaca atttcaaaa taatgagta ttttgaata 
      cgatgcgat atactgagt gtcttctac tcgaaatgg 
      ttcgaacgt tagaacgca cggaaccga tcctccaag 
      cccagatcg acgaagcca acactttct tgaacagaa 
      tgaagcaag cggcgaaga agtggccgt tcttcaggc 
      cgagatttt ggcatttga aggcgtata atgcaagga 
      tttttaata agttgcaag cgactatta agtttctga 
      agactgcta attcgtttg cggccgagg atgggaata 
      ttttggagg agtgctgga ctatgttgc atgcaacgt 
      cttggttcg gagggtttt cgtcggata aagagccca 
      aacatctta aatgggagt tggcaaaga cctacacgc 
      cgttgcagt ggtgtcaga ttttgtacg ccctaccgg 
      ctgaatagc ttttttctt gtttaagtg ttaactgtt 
      aataaatac gaattttgt gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY