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Gene information
General information
TC008062 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4434692..4435823
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008062
    iBeetle screen iB_04421

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): more than 80%

    antenna mostlyremaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    labrum mostly remaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    gut remaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus mostly remaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008062 RA
      MVKREETIL VIPTMARTP LKSSFSISS ILPETALEA 
      PKSPENHPE TELSDSDCD LDVTGTPPP LDCSKNSPE 
      KESEEKKSK EKPPYSYNA LIMMAIRNS PEKRLTLNG 
      IYEYIMRNF PYYRENKQG WQNSIRHNL SLNKCFVKV 
      PRHYDDPGK GNYWMLDPS AEDVFIGGT TGKLRRRST 
      AASRSRLAA FKRTIALGA FYQPLQPFP PPYPFYPGT 
      SAMLAAYQR YNPYLQAAS MLPKPTPLV PQQGFNMER 
      LLAPSTEVS APFFRQPLD LYNFRVPQH QVLQSPSST 
      SSSPEPRSP ESLFKPVTV ISRQS
    CDS sequence
    • >TC008062 RA
      atggtgaaa agggaagaa acgattttg gttattccg 
      acgatggcc cgaactccg ctcaaatcg tcgttttcg 
      atcagttcc atcctacct gagacggct ctggaagcg 
      cccaagagc ccggaaaac cacccggaa acggaactg 
      agcgactcg gactgtgac ttggacgtt accggaacg 
      ccaccgccg ctcgactgc tcgaagaac tccccggaa 
      aaggagtcc gaagagaag aaaagcaaa gagaaacct 
      ccgtacagt tacaacgca cttatcatg atggcgatc 
      cggaatagt ccggagaaa agactcact cttaacggc 
      atctacgag tacataatg cgcaacttt ccgtactac 
      cgtgaaaac aagcaaggt tggcaaaac tccatccgg 
      cacaactta agtctgaac aaatgcttc gtcaaagtc 
      ccgcgacat tacgacgac cccggaaag ggcaattac 
      tggatgctt gacccttcc gccgaagac gtgtttatc 
      ggcggcacc accggaaag ctccggagg cggtcgacc 
      gccgcctcc aggtcccgg ctcgccgcc ttcaagcgt 
      accatcgcc ttgggcgcg ttctaccag cccctgcag 
      ccattcccg cccccgtat cccttttac cccggaact 
      tcggcaatg ttggcggcc taccaacgg tacaacccc 
      tacctgcaa gccgcctcc atgctcccg aagcccacg 
      ccactcgtg ccccagcaa ggcttcaac atggagcgg 
      ctgctagcc ccctcgacg gaggtctca gcgccgttt 
      ttccgacaa ccgctcgat ttgtacaat ttccgggtg 
      ccccagcac caagtgctg caatcgccg tcgagcacg 
      tccagttca ccggagccc cgcagtccc gaaagtctg 
      tttaaacca gtgacggtg atctcgagg cagagttga
    mRNA sequence
    • >TC008062 RA
      gcttgagtc aagacgtgt tctctcaca gtgtttgtt 
      atgtaaata tggtgaaaa gggaagaaa cgattttgg 
      ttattccga cgatggccc gaactccgc tcaaatcgt 
      cgttttcga tcagttcca tcctacctg agacggctc 
      tggaagcgc ccaagagcc cggaaaacc acccggaaa 
      cggaactga gcgactcgg actgtgact tggacgtta 
      ccggaacgc caccgccgc tcgactgct cgaagaact 
      ccccggaaa aggagtccg aagagaaga aaagcaaag 
      agaaacctc cgtacagtt acaacgcac ttatcatga 
      tggcgatcc ggaatagtc cggagaaaa gactcactc 
      ttaacggca tctacgagt acataatgc gcaactttc 
      cgtactacc gtgaaaaca agcaaggtt ggcaaaact 
      ccatccggc acaacttaa gtctgaaca aatgcttcg 
      tcaaagtcc cgcgacatt acgacgacc ccggaaagg 
      gcaattact ggatgcttg acccttccg ccgaagacg 
      tgtttatcg gcggcacca ccggaaagc tccggaggc 
      ggtcgaccg ccgcctcca ggtcccggc tcgccgcct 
      tcaagcgta ccatcgcct tgggcgcgt tctaccagc 
      ccctgcagc cattcccgc ccccgtatc ccttttacc 
      ccggaactt cggcaatgt tggcggcct accaacggt 
      acaacccct acctgcaag ccgcctcca tgctcccga 
      agcccacgc cactcgtgc cccagcaag gcttcaaca 
      tggagcggc tgctagccc cctcgacgg aggtctcag 
      cgccgtttt tccgacaac cgctcgatt tgtacaatt 
      tccgggtgc cccagcacc aagtgctgc aatcgccgt 
      cgagcacgt ccagttcac cggagcccc gcagtcccg 
      aaagtctgt ttaaaccag tgacggtga tctcgaggc 
      agagttgat ggtgattgt ggccgagtt tttgatctc 
      attagaaaa acgtccagt tttgtacat atttgtata 
      ttcaagtag gttttaaga tagaatgtg atgtacctt 
      tgtagaaat gtatagtgt atatgagat atttaaata 
      tatatttga acgagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY