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Gene information
General information
TC008047 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4295702..4299028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC008047
      iBeetle screen iB_04420

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection

      Adult morphology
      abdominal odoriferous glands mostly content decreased - Number of animals showing the phenotype: 3 (13 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008047 RA
        MAVLKILFL FSLHICAPN SVVCGPTED YNYSLEDEQ 
NSVNFTHVR AKNLSGNVQ LTSLEVLRT EMLNNGFGK 
VEETTKVTV SVNKHIGTI IENQQQDSL LSEVGTKAP 
TDGLEPIGL MDVFNDTSG TLPDQTTTE KLITDTPLN 
SEITEKSQD WVSGDLSLI STVRSERIN SVDDERVAL 
IRGLISESE EIENLSTFL EVDEELLNE HEDEKDVEG 
AIEDDINNI LTEYEDNEE EINQNKTET GSQAVREST 
TIFTQEKDE TVASTFTTE EISSTTQNS EKEIFKQTT 
IWKKFYLDE ETTTGFFTT FSENISTEK LYETTQKEE 
NAVTATDIT SQGKETTEF AVAEDFNAM STAKEEIYM 
TPTESTTYN FLIAKTENE EIKIAEETE ASTPNYEND 
GGRKFETSR INEESLSTE MFTEEPSEN ITLLLNKLE 
ETELSTSTT ELYTTIFGK NTERNSNIT ETMEIFTEN 
QTEIVQNQE GNLATTPLY EKELEQLEE IIDNITTTL 
NKYEEIQVF TYEQQTTQF QQQFDTTTE HEESKRKAE 
KEKFAMKEA EEDQIKNKI FTKSPEILR EGWKNNTED 
KLENINEIT NEENFDKGH ISEKLATEI RGENNAEKK 
EENINTNEA SDDILPTTN FILTTNKTQ EVLSTTTEE 
NEDFVSVST PTNTTETEI NGGFLNSLT SGISSFLGT 
FFDSAEKQF EEKNFGDED MKNKFEEDK YNDIFNDET 
EETKEDNKI NHFTKLKVK EPYRNYLNQ FEEETTTIN 
LNLEDLNSA SKSPHLPSK ADSLINSPE VPQILIKIN 
DEETKVVIK IITNDSTSY IPSNALVSF PSPNTVNIT 
WKSSSFRNN SLQFVMGNS LRLVIVKDT EIYIHQTLI 
HENKFDVDT NLITLDDDV QIYFSNRDI LGLDQRIKT 
EFSEMTSGG KNLAEITGG CVGAFLLVA GLAYFITKK 
LRGEKQNFE PNTAGA
      CDS sequence
      • >TC008047 RA
        atggcagtg ttaaaaata cttttctta ttttcactt 
cacatttgt gccccaaat tcggttgtt tgcggaccg 
actgaagat tataattac tcgttggaa gatgagcaa 
aattcggtt aatttcaca cacgtgcgt gcgaaaaat 
ttgagcggg aatgtgcag ttgacgtcg cttgaagtt 
ttgcgaaca gaaatgtta aacaatggc tttgggaaa 
gtcgaagag accaccaaa gtaacagtc agtgtaaat 
aaacacatt ggaacaatc atcgaaaat caacaacaa 
gactcttta ttgtccgaa gttgggaca aaagctccg 
acagatggt ctggaaccg atcggtttg atggatgtt 
tttaacgac acatctggc actttgccc gatcagacc 
actacagaa aaactaatc actgacact ccattaaat 
tcagagatc actgaaaaa tcccaagat tgggtttca 
ggtgatttg agtttaatt tcgacggtt cgaagtgaa 
agaattaat tcagtggat gatgagaga gttgccttg 
attagggga ctgatttca gagagtgaa gaaattgag 
aatttgtcc acgttttta gaggttgat gaagaattg 
ctaaatgaa catgaggat gagaaggac gtggagggg 
gcgattgag gacgacatt aataatatt ttgacggaa 
tacgaagac aatgaagaa gaaataaac caaaataaa 
acagaaact gggtcacaa gcagttaga gaaagcacc 
acaattttt actcaagag aaagacgaa acggtggct 
tcaacgttt acaactgaa gaaatttcc tcgactaca 
caaaatagt gaaaaagaa attttcaaa caaacaaca 
atttggaag aaattttat ctagatgaa gaaacaacc 
acgggattt ttcacaact ttttccgaa aatatttct 
acagaaaaa ctttacgaa acgacacaa aaagaagaa 
aatgctgta actgcaaca gatataaca agtcaggga 
aaagaaaca acagagttt gcagtagct gaagatttt 
aatgcgatg tcaacagca aaagaagaa atttatatg 
actcctaca gaaagtaca acttataat tttcttatt 
gcaaaaact gaaaatgaa gaaattaaa attgctgaa 
gaaactgaa gcatcgaca ccgaattat gaaaacgat 
ggaggaaga aaatttgaa acgagccga ataaatgaa 
gaaagtttg tccactgaa atgtttaca gaagaaccc 
tccgaaaat attacatta cttttaaat aaacttgaa 
gaaactgaa ctatcgaca tcaacaacc gaactttac 
acaacaatt tttggaaaa aatactgaa agaaactcg 
aatattacc gaaactatg gaaattttt acagaaaat 
caaactgaa attgtacaa aatcaggaa gggaattta 
gctacaaca ccactttat gagaaggaa ttagaacaa 
ttagaagaa attattgat aacattaca acaactttg 
aataaatac gaagaaatt caagttttc acgtatgaa 
cagcaaaca acacagttt cagcaacag tttgacacc 
accacagaa catgaagaa agcaagaga aaggcagaa 
aaagaaaag tttgcaatg aaagaagca gaggaagac 
caaattaaa aataaaatc tttacaaaa tccccagaa 
attttaaga gaaggttgg aaaaacaac acggaagac 
aagcttgaa aatataaat gaaataact aatgaggaa 
aattttgat aaaggtcac atatcagaa aaattagca 
actgaaata cgaggtgaa aacaatgcc gaaaaaaaa 
gaagaaaat attaacaca aatgaagca agtgatgat 
attttacca actacaaat tttatttta accacaaat 
aaaacccaa gaagtactt tccacaacc actgaagaa 
aatgaagat tttgtgtca gtttccact cccactaac 
acaacagaa accgaaata aacggaggt tttttgaat 
tcgttaaca tcaggaatt tctagtttc cttggaact 
tttttcgat tcagcagaa aaacaattc gaggaaaaa 
aatttcggt gacgaagac atgaaaaat aaatttgag 
gaagataaa tataacgac atattcaac gacgagact 
gaagaaacc aaagaagat aataaaatt aatcatttt 
actaaacta aaagttaaa gaaccgtac agaaattat 
ttgaatcag tttgaggaa gaaacgaca acgataaac 
ttaaacttg gaggatttg aattcagcc tcaaaatca 
cctcatttg ccctcgaaa gccgacagt ttgatcaat 
tctccagaa gtgccccaa attttgatt aaaatcaac 
gacgaagaa acaaaagtt gttatcaaa attatcacc 
aacgactcg acctcgtac atcccctca aacgccctc 
gtctcattt ccatccccg aacaccgtc aacatcacg 
tggaaatcg tcatcgttt cgaaacaat tctttacaa 
tttgttatg ggaaattct ctccgcctc gttattgtc 
aaagacacc gaaatatac atccaccag acacttatc 
cacgaaaat aaattcgat gttgacacg aacttaatc 
actttagac gacgacgtc caaatttat ttctctaat 
agggacatc ttgggatta gatcaacga atcaagacc 
gaattttcg gaaatgaca agtgggggc aaaaacttg 
gccgagatt accggaggc tgcgtgggg gcgtttctg 
ctggtcgca ggtcttgcc tatttcatc accaagaag 
ttgagagga gagaaacag aatttcgaa cccaacacc 
gcaggggca tag
      mRNA sequence
      • >TC008047 RA
        gaggttgta aaattttct gcgaatcgg agccacgta 
ttgtgtcta aatggctca agaccgccc taaagccat 
tgatgacag taaaaactc aagacgtaa cggatagtc 
tccagacag gggtgctct tgtgatcgg tccagacgt 
tcatctagt cgaagtgaa gcaagtggt gtgctagct 
atggcagtg ttaaaaata cttttctta ttttcactt 
cacatttgt gccccaaat tcggttgtt tgcggaccg 
actgaagat tataattac tcgttggaa gatgagcaa 
aattcggtt aatttcaca cacgtgcgt gcgaaaaat 
ttgagcggg aatgtgcag ttgacgtcg cttgaagtt 
ttgcgaaca gaaatgtta aacaatggc tttgggaaa 
gtcgaagag accaccaaa gtaacagtc agtgtaaat 
aaacacatt ggaacaatc atcgaaaat caacaacaa 
gactcttta ttgtccgaa gttgggaca aaagctccg 
acagatggt ctggaaccg atcggtttg atggatgtt 
tttaacgac acatctggc actttgccc gatcagacc 
actacagaa aaactaatc actgacact ccattaaat 
tcagagatc actgaaaaa tcccaagat tgggtttca 
ggtgatttg agtttaatt tcgacggtt cgaagtgaa 
agaattaat tcagtggat gatgagaga gttgccttg 
attagggga ctgatttca gagagtgaa gaaattgag 
aatttgtcc acgttttta gaggttgat gaagaattg 
ctaaatgaa catgaggat gagaaggac gtggagggg 
gcgattgag gacgacatt aataatatt ttgacggaa 
tacgaagac aatgaagaa gaaataaac caaaataaa 
acagaaact gggtcacaa gcagttaga gaaagcacc 
acaattttt actcaagag aaagacgaa acggtggct 
tcaacgttt acaactgaa gaaatttcc tcgactaca 
caaaatagt gaaaaagaa attttcaaa caaacaaca 
atttggaag aaattttat ctagatgaa gaaacaacc 
acgggattt ttcacaact ttttccgaa aatatttct 
acagaaaaa ctttacgaa acgacacaa aaagaagaa 
aatgctgta actgcaaca gatataaca agtcaggga 
aaagaaaca acagagttt gcagtagct gaagatttt 
aatgcgatg tcaacagca aaagaagaa atttatatg 
actcctaca gaaagtaca acttataat tttcttatt 
gcaaaaact gaaaatgaa gaaattaaa attgctgaa 
gaaactgaa gcatcgaca ccgaattat gaaaacgat 
ggaggaaga aaatttgaa acgagccga ataaatgaa 
gaaagtttg tccactgaa atgtttaca gaagaaccc 
tccgaaaat attacatta cttttaaat aaacttgaa 
gaaactgaa ctatcgaca tcaacaacc gaactttac 
acaacaatt tttggaaaa aatactgaa agaaactcg 
aatattacc gaaactatg gaaattttt acagaaaat 
caaactgaa attgtacaa aatcaggaa gggaattta 
gctacaaca ccactttat gagaaggaa ttagaacaa 
ttagaagaa attattgat aacattaca acaactttg 
aataaatac gaagaaatt caagttttc acgtatgaa 
cagcaaaca acacagttt cagcaacag tttgacacc 
accacagaa catgaagaa agcaagaga aaggcagaa 
aaagaaaag tttgcaatg aaagaagca gaggaagac 
caaattaaa aataaaatc tttacaaaa tccccagaa 
attttaaga gaaggttgg aaaaacaac acggaagac 
aagcttgaa aatataaat gaaataact aatgaggaa 
aattttgat aaaggtcac atatcagaa aaattagca 
actgaaata cgaggtgaa aacaatgcc gaaaaaaaa 
gaagaaaat attaacaca aatgaagca agtgatgat 
attttacca actacaaat tttatttta accacaaat 
aaaacccaa gaagtactt tccacaacc actgaagaa 
aatgaagat tttgtgtca gtttccact cccactaac 
acaacagaa accgaaata aacggaggt tttttgaat 
tcgttaaca tcaggaatt tctagtttc cttggaact 
tttttcgat tcagcagaa aaacaattc gaggaaaaa 
aatttcggt gacgaagac atgaaaaat aaatttgag 
gaagataaa tataacgac atattcaac gacgagact 
gaagaaacc aaagaagat aataaaatt aatcatttt 
actaaacta aaagttaaa gaaccgtac agaaattat 
ttgaatcag tttgaggaa gaaacgaca acgataaac 
ttaaacttg gaggatttg aattcagcc tcaaaatca 
cctcatttg ccctcgaaa gccgacagt ttgatcaat 
tctccagaa gtgccccaa attttgatt aaaatcaac 
gacgaagaa acaaaagtt gttatcaaa attatcacc 
aacgactcg acctcgtac atcccctca aacgccctc 
gtctcattt ccatccccg aacaccgtc aacatcacg 
tggaaatcg tcatcgttt cgaaacaat tctttacaa 
tttgttatg ggaaattct ctccgcctc gttattgtc 
aaagacacc gaaatatac atccaccag acacttatc 
cacgaaaat aaattcgat gttgacacg aacttaatc 
actttagac gacgacgtc caaatttat ttctctaat 
agggacatc ttgggatta gatcaacga atcaagacc 
gaattttcg gaaatgaca agtgggggc aaaaacttg 
gccgagatt accggaggc tgcgtgggg gcgtttctg 
ctggtcgca ggtcttgcc tatttcatc accaagaag 
ttgagagga gagaaacag aatttcgaa cccaacacc 
gcaggggca tagttcagt gtgatgcaa accaaagaa 
actttatct ttgtagaac aatatttac gtaaatcac 
tttattgta tataaaaac ctcgttctt ttgtaataa 
atttttata attact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY