Status
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Gene information
General information
TC008047 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4295702..4299028
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008047
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04420

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Adult morphology
      abdominal odoriferous glands mostly content decreased - Number of animals showing the phenotype: 3 (13 dpi)
      Days post injection



      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008047 RA
        MAVLKILFL FSLHICAPN SVVCGPTED YNYSLEDEQ 
        NSVNFTHVR AKNLSGNVQ LTSLEVLRT EMLNNGFGK 
        VEETTKVTV SVNKHIGTI IENQQQDSL LSEVGTKAP 
        TDGLEPIGL MDVFNDTSG TLPDQTTTE KLITDTPLN 
        SEITEKSQD WVSGDLSLI STVRSERIN SVDDERVAL 
        IRGLISESE EIENLSTFL EVDEELLNE HEDEKDVEG 
        AIEDDINNI LTEYEDNEE EINQNKTET GSQAVREST 
        TIFTQEKDE TVASTFTTE EISSTTQNS EKEIFKQTT 
        IWKKFYLDE ETTTGFFTT FSENISTEK LYETTQKEE 
        NAVTATDIT SQGKETTEF AVAEDFNAM STAKEEIYM 
        TPTESTTYN FLIAKTENE EIKIAEETE ASTPNYEND 
        GGRKFETSR INEESLSTE MFTEEPSEN ITLLLNKLE 
        ETELSTSTT ELYTTIFGK NTERNSNIT ETMEIFTEN 
        QTEIVQNQE GNLATTPLY EKELEQLEE IIDNITTTL 
        NKYEEIQVF TYEQQTTQF QQQFDTTTE HEESKRKAE 
        KEKFAMKEA EEDQIKNKI FTKSPEILR EGWKNNTED 
        KLENINEIT NEENFDKGH ISEKLATEI RGENNAEKK 
        EENINTNEA SDDILPTTN FILTTNKTQ EVLSTTTEE 
        NEDFVSVST PTNTTETEI NGGFLNSLT SGISSFLGT 
        FFDSAEKQF EEKNFGDED MKNKFEEDK YNDIFNDET 
        EETKEDNKI NHFTKLKVK EPYRNYLNQ FEEETTTIN 
        LNLEDLNSA SKSPHLPSK ADSLINSPE VPQILIKIN 
        DEETKVVIK IITNDSTSY IPSNALVSF PSPNTVNIT 
        WKSSSFRNN SLQFVMGNS LRLVIVKDT EIYIHQTLI 
        HENKFDVDT NLITLDDDV QIYFSNRDI LGLDQRIKT 
        EFSEMTSGG KNLAEITGG CVGAFLLVA GLAYFITKK 
        LRGEKQNFE PNTAGA
      CDS sequence
      • >TC008047 RA
        atggcagtg ttaaaaata cttttctta ttttcactt 
        cacatttgt gccccaaat tcggttgtt tgcggaccg 
        actgaagat tataattac tcgttggaa gatgagcaa 
        aattcggtt aatttcaca cacgtgcgt gcgaaaaat 
        ttgagcggg aatgtgcag ttgacgtcg cttgaagtt 
        ttgcgaaca gaaatgtta aacaatggc tttgggaaa 
        gtcgaagag accaccaaa gtaacagtc agtgtaaat 
        aaacacatt ggaacaatc atcgaaaat caacaacaa 
        gactcttta ttgtccgaa gttgggaca aaagctccg 
        acagatggt ctggaaccg atcggtttg atggatgtt 
        tttaacgac acatctggc actttgccc gatcagacc 
        actacagaa aaactaatc actgacact ccattaaat 
        tcagagatc actgaaaaa tcccaagat tgggtttca 
        ggtgatttg agtttaatt tcgacggtt cgaagtgaa 
        agaattaat tcagtggat gatgagaga gttgccttg 
        attagggga ctgatttca gagagtgaa gaaattgag 
        aatttgtcc acgttttta gaggttgat gaagaattg 
        ctaaatgaa catgaggat gagaaggac gtggagggg 
        gcgattgag gacgacatt aataatatt ttgacggaa 
        tacgaagac aatgaagaa gaaataaac caaaataaa 
        acagaaact gggtcacaa gcagttaga gaaagcacc 
        acaattttt actcaagag aaagacgaa acggtggct 
        tcaacgttt acaactgaa gaaatttcc tcgactaca 
        caaaatagt gaaaaagaa attttcaaa caaacaaca 
        atttggaag aaattttat ctagatgaa gaaacaacc 
        acgggattt ttcacaact ttttccgaa aatatttct 
        acagaaaaa ctttacgaa acgacacaa aaagaagaa 
        aatgctgta actgcaaca gatataaca agtcaggga 
        aaagaaaca acagagttt gcagtagct gaagatttt 
        aatgcgatg tcaacagca aaagaagaa atttatatg 
        actcctaca gaaagtaca acttataat tttcttatt 
        gcaaaaact gaaaatgaa gaaattaaa attgctgaa 
        gaaactgaa gcatcgaca ccgaattat gaaaacgat 
        ggaggaaga aaatttgaa acgagccga ataaatgaa 
        gaaagtttg tccactgaa atgtttaca gaagaaccc 
        tccgaaaat attacatta cttttaaat aaacttgaa 
        gaaactgaa ctatcgaca tcaacaacc gaactttac 
        acaacaatt tttggaaaa aatactgaa agaaactcg 
        aatattacc gaaactatg gaaattttt acagaaaat 
        caaactgaa attgtacaa aatcaggaa gggaattta 
        gctacaaca ccactttat gagaaggaa ttagaacaa 
        ttagaagaa attattgat aacattaca acaactttg 
        aataaatac gaagaaatt caagttttc acgtatgaa 
        cagcaaaca acacagttt cagcaacag tttgacacc 
        accacagaa catgaagaa agcaagaga aaggcagaa 
        aaagaaaag tttgcaatg aaagaagca gaggaagac 
        caaattaaa aataaaatc tttacaaaa tccccagaa 
        attttaaga gaaggttgg aaaaacaac acggaagac 
        aagcttgaa aatataaat gaaataact aatgaggaa 
        aattttgat aaaggtcac atatcagaa aaattagca 
        actgaaata cgaggtgaa aacaatgcc gaaaaaaaa 
        gaagaaaat attaacaca aatgaagca agtgatgat 
        attttacca actacaaat tttatttta accacaaat 
        aaaacccaa gaagtactt tccacaacc actgaagaa 
        aatgaagat tttgtgtca gtttccact cccactaac 
        acaacagaa accgaaata aacggaggt tttttgaat 
        tcgttaaca tcaggaatt tctagtttc cttggaact 
        tttttcgat tcagcagaa aaacaattc gaggaaaaa 
        aatttcggt gacgaagac atgaaaaat aaatttgag 
        gaagataaa tataacgac atattcaac gacgagact 
        gaagaaacc aaagaagat aataaaatt aatcatttt 
        actaaacta aaagttaaa gaaccgtac agaaattat 
        ttgaatcag tttgaggaa gaaacgaca acgataaac 
        ttaaacttg gaggatttg aattcagcc tcaaaatca 
        cctcatttg ccctcgaaa gccgacagt ttgatcaat 
        tctccagaa gtgccccaa attttgatt aaaatcaac 
        gacgaagaa acaaaagtt gttatcaaa attatcacc 
        aacgactcg acctcgtac atcccctca aacgccctc 
        gtctcattt ccatccccg aacaccgtc aacatcacg 
        tggaaatcg tcatcgttt cgaaacaat tctttacaa 
        tttgttatg ggaaattct ctccgcctc gttattgtc 
        aaagacacc gaaatatac atccaccag acacttatc 
        cacgaaaat aaattcgat gttgacacg aacttaatc 
        actttagac gacgacgtc caaatttat ttctctaat 
        agggacatc ttgggatta gatcaacga atcaagacc 
        gaattttcg gaaatgaca agtgggggc aaaaacttg 
        gccgagatt accggaggc tgcgtgggg gcgtttctg 
        ctggtcgca ggtcttgcc tatttcatc accaagaag 
        ttgagagga gagaaacag aatttcgaa cccaacacc 
        gcaggggca tag
      mRNA sequence
      • >TC008047 RA
        gaggttgta aaattttct gcgaatcgg agccacgta 
        ttgtgtcta aatggctca agaccgccc taaagccat 
        tgatgacag taaaaactc aagacgtaa cggatagtc 
        tccagacag gggtgctct tgtgatcgg tccagacgt 
        tcatctagt cgaagtgaa gcaagtggt gtgctagct 
        atggcagtg ttaaaaata cttttctta ttttcactt 
        cacatttgt gccccaaat tcggttgtt tgcggaccg 
        actgaagat tataattac tcgttggaa gatgagcaa 
        aattcggtt aatttcaca cacgtgcgt gcgaaaaat 
        ttgagcggg aatgtgcag ttgacgtcg cttgaagtt 
        ttgcgaaca gaaatgtta aacaatggc tttgggaaa 
        gtcgaagag accaccaaa gtaacagtc agtgtaaat 
        aaacacatt ggaacaatc atcgaaaat caacaacaa 
        gactcttta ttgtccgaa gttgggaca aaagctccg 
        acagatggt ctggaaccg atcggtttg atggatgtt 
        tttaacgac acatctggc actttgccc gatcagacc 
        actacagaa aaactaatc actgacact ccattaaat 
        tcagagatc actgaaaaa tcccaagat tgggtttca 
        ggtgatttg agtttaatt tcgacggtt cgaagtgaa 
        agaattaat tcagtggat gatgagaga gttgccttg 
        attagggga ctgatttca gagagtgaa gaaattgag 
        aatttgtcc acgttttta gaggttgat gaagaattg 
        ctaaatgaa catgaggat gagaaggac gtggagggg 
        gcgattgag gacgacatt aataatatt ttgacggaa 
        tacgaagac aatgaagaa gaaataaac caaaataaa 
        acagaaact gggtcacaa gcagttaga gaaagcacc 
        acaattttt actcaagag aaagacgaa acggtggct 
        tcaacgttt acaactgaa gaaatttcc tcgactaca 
        caaaatagt gaaaaagaa attttcaaa caaacaaca 
        atttggaag aaattttat ctagatgaa gaaacaacc 
        acgggattt ttcacaact ttttccgaa aatatttct 
        acagaaaaa ctttacgaa acgacacaa aaagaagaa 
        aatgctgta actgcaaca gatataaca agtcaggga 
        aaagaaaca acagagttt gcagtagct gaagatttt 
        aatgcgatg tcaacagca aaagaagaa atttatatg 
        actcctaca gaaagtaca acttataat tttcttatt 
        gcaaaaact gaaaatgaa gaaattaaa attgctgaa 
        gaaactgaa gcatcgaca ccgaattat gaaaacgat 
        ggaggaaga aaatttgaa acgagccga ataaatgaa 
        gaaagtttg tccactgaa atgtttaca gaagaaccc 
        tccgaaaat attacatta cttttaaat aaacttgaa 
        gaaactgaa ctatcgaca tcaacaacc gaactttac 
        acaacaatt tttggaaaa aatactgaa agaaactcg 
        aatattacc gaaactatg gaaattttt acagaaaat 
        caaactgaa attgtacaa aatcaggaa gggaattta 
        gctacaaca ccactttat gagaaggaa ttagaacaa 
        ttagaagaa attattgat aacattaca acaactttg 
        aataaatac gaagaaatt caagttttc acgtatgaa 
        cagcaaaca acacagttt cagcaacag tttgacacc 
        accacagaa catgaagaa agcaagaga aaggcagaa 
        aaagaaaag tttgcaatg aaagaagca gaggaagac 
        caaattaaa aataaaatc tttacaaaa tccccagaa 
        attttaaga gaaggttgg aaaaacaac acggaagac 
        aagcttgaa aatataaat gaaataact aatgaggaa 
        aattttgat aaaggtcac atatcagaa aaattagca 
        actgaaata cgaggtgaa aacaatgcc gaaaaaaaa 
        gaagaaaat attaacaca aatgaagca agtgatgat 
        attttacca actacaaat tttatttta accacaaat 
        aaaacccaa gaagtactt tccacaacc actgaagaa 
        aatgaagat tttgtgtca gtttccact cccactaac 
        acaacagaa accgaaata aacggaggt tttttgaat 
        tcgttaaca tcaggaatt tctagtttc cttggaact 
        tttttcgat tcagcagaa aaacaattc gaggaaaaa 
        aatttcggt gacgaagac atgaaaaat aaatttgag 
        gaagataaa tataacgac atattcaac gacgagact 
        gaagaaacc aaagaagat aataaaatt aatcatttt 
        actaaacta aaagttaaa gaaccgtac agaaattat 
        ttgaatcag tttgaggaa gaaacgaca acgataaac 
        ttaaacttg gaggatttg aattcagcc tcaaaatca 
        cctcatttg ccctcgaaa gccgacagt ttgatcaat 
        tctccagaa gtgccccaa attttgatt aaaatcaac 
        gacgaagaa acaaaagtt gttatcaaa attatcacc 
        aacgactcg acctcgtac atcccctca aacgccctc 
        gtctcattt ccatccccg aacaccgtc aacatcacg 
        tggaaatcg tcatcgttt cgaaacaat tctttacaa 
        tttgttatg ggaaattct ctccgcctc gttattgtc 
        aaagacacc gaaatatac atccaccag acacttatc 
        cacgaaaat aaattcgat gttgacacg aacttaatc 
        actttagac gacgacgtc caaatttat ttctctaat 
        agggacatc ttgggatta gatcaacga atcaagacc 
        gaattttcg gaaatgaca agtgggggc aaaaacttg 
        gccgagatt accggaggc tgcgtgggg gcgtttctg 
        ctggtcgca ggtcttgcc tatttcatc accaagaag 
        ttgagagga gagaaacag aatttcgaa cccaacacc 
        gcaggggca tagttcagt gtgatgcaa accaaagaa 
        actttatct ttgtagaac aatatttac gtaaatcac 
        tttattgta tataaaaac ctcgttctt ttgtaataa 
        atttttata attact
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY