| General information | |||
| TC008047 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 4295702..4299028 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC008047 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04420 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008047 RA
MAVLKILFL FSLHICAPN SVVCGPTED YNYSLEDEQ NSVNFTHVR AKNLSGNVQ LTSLEVLRT EMLNNGFGK VEETTKVTV SVNKHIGTI IENQQQDSL LSEVGTKAP TDGLEPIGL MDVFNDTSG TLPDQTTTE KLITDTPLN SEITEKSQD WVSGDLSLI STVRSERIN SVDDERVAL IRGLISESE EIENLSTFL EVDEELLNE HEDEKDVEG AIEDDINNI LTEYEDNEE EINQNKTET GSQAVREST TIFTQEKDE TVASTFTTE EISSTTQNS EKEIFKQTT IWKKFYLDE ETTTGFFTT FSENISTEK LYETTQKEE NAVTATDIT SQGKETTEF AVAEDFNAM STAKEEIYM TPTESTTYN FLIAKTENE EIKIAEETE ASTPNYEND GGRKFETSR INEESLSTE MFTEEPSEN ITLLLNKLE ETELSTSTT ELYTTIFGK NTERNSNIT ETMEIFTEN QTEIVQNQE GNLATTPLY EKELEQLEE IIDNITTTL NKYEEIQVF TYEQQTTQF QQQFDTTTE HEESKRKAE KEKFAMKEA EEDQIKNKI FTKSPEILR EGWKNNTED KLENINEIT NEENFDKGH ISEKLATEI RGENNAEKK EENINTNEA SDDILPTTN FILTTNKTQ EVLSTTTEE NEDFVSVST PTNTTETEI NGGFLNSLT SGISSFLGT FFDSAEKQF EEKNFGDED MKNKFEEDK YNDIFNDET EETKEDNKI NHFTKLKVK EPYRNYLNQ FEEETTTIN LNLEDLNSA SKSPHLPSK ADSLINSPE VPQILIKIN DEETKVVIK IITNDSTSY IPSNALVSF PSPNTVNIT WKSSSFRNN SLQFVMGNS LRLVIVKDT EIYIHQTLI HENKFDVDT NLITLDDDV QIYFSNRDI LGLDQRIKT EFSEMTSGG KNLAEITGG CVGAFLLVA GLAYFITKK LRGEKQNFE PNTAGA
- >TC008047 RA
atggcagtg ttaaaaata cttttctta ttttcactt cacatttgt gccccaaat tcggttgtt tgcggaccg actgaagat tataattac tcgttggaa gatgagcaa aattcggtt aatttcaca cacgtgcgt gcgaaaaat ttgagcggg aatgtgcag ttgacgtcg cttgaagtt ttgcgaaca gaaatgtta aacaatggc tttgggaaa gtcgaagag accaccaaa gtaacagtc agtgtaaat aaacacatt ggaacaatc atcgaaaat caacaacaa gactcttta ttgtccgaa gttgggaca aaagctccg acagatggt ctggaaccg atcggtttg atggatgtt tttaacgac acatctggc actttgccc gatcagacc actacagaa aaactaatc actgacact ccattaaat tcagagatc actgaaaaa tcccaagat tgggtttca ggtgatttg agtttaatt tcgacggtt cgaagtgaa agaattaat tcagtggat gatgagaga gttgccttg attagggga ctgatttca gagagtgaa gaaattgag aatttgtcc acgttttta gaggttgat gaagaattg ctaaatgaa catgaggat gagaaggac gtggagggg gcgattgag gacgacatt aataatatt ttgacggaa tacgaagac aatgaagaa gaaataaac caaaataaa acagaaact gggtcacaa gcagttaga gaaagcacc acaattttt actcaagag aaagacgaa acggtggct tcaacgttt acaactgaa gaaatttcc tcgactaca caaaatagt gaaaaagaa attttcaaa caaacaaca atttggaag aaattttat ctagatgaa gaaacaacc acgggattt ttcacaact ttttccgaa aatatttct acagaaaaa ctttacgaa acgacacaa aaagaagaa aatgctgta actgcaaca gatataaca agtcaggga aaagaaaca acagagttt gcagtagct gaagatttt aatgcgatg tcaacagca aaagaagaa atttatatg actcctaca gaaagtaca acttataat tttcttatt gcaaaaact gaaaatgaa gaaattaaa attgctgaa gaaactgaa gcatcgaca ccgaattat gaaaacgat ggaggaaga aaatttgaa acgagccga ataaatgaa gaaagtttg tccactgaa atgtttaca gaagaaccc tccgaaaat attacatta cttttaaat aaacttgaa gaaactgaa ctatcgaca tcaacaacc gaactttac acaacaatt tttggaaaa aatactgaa agaaactcg aatattacc gaaactatg gaaattttt acagaaaat caaactgaa attgtacaa aatcaggaa gggaattta gctacaaca ccactttat gagaaggaa ttagaacaa ttagaagaa attattgat aacattaca acaactttg aataaatac gaagaaatt caagttttc acgtatgaa cagcaaaca acacagttt cagcaacag tttgacacc accacagaa catgaagaa agcaagaga aaggcagaa aaagaaaag tttgcaatg aaagaagca gaggaagac caaattaaa aataaaatc tttacaaaa tccccagaa attttaaga gaaggttgg aaaaacaac acggaagac aagcttgaa aatataaat gaaataact aatgaggaa aattttgat aaaggtcac atatcagaa aaattagca actgaaata cgaggtgaa aacaatgcc gaaaaaaaa gaagaaaat attaacaca aatgaagca agtgatgat attttacca actacaaat tttatttta accacaaat aaaacccaa gaagtactt tccacaacc actgaagaa aatgaagat tttgtgtca gtttccact cccactaac acaacagaa accgaaata aacggaggt tttttgaat tcgttaaca tcaggaatt tctagtttc cttggaact tttttcgat tcagcagaa aaacaattc gaggaaaaa aatttcggt gacgaagac atgaaaaat aaatttgag gaagataaa tataacgac atattcaac gacgagact gaagaaacc aaagaagat aataaaatt aatcatttt actaaacta aaagttaaa gaaccgtac agaaattat ttgaatcag tttgaggaa gaaacgaca acgataaac ttaaacttg gaggatttg aattcagcc tcaaaatca cctcatttg ccctcgaaa gccgacagt ttgatcaat tctccagaa gtgccccaa attttgatt aaaatcaac gacgaagaa acaaaagtt gttatcaaa attatcacc aacgactcg acctcgtac atcccctca aacgccctc gtctcattt ccatccccg aacaccgtc aacatcacg tggaaatcg tcatcgttt cgaaacaat tctttacaa tttgttatg ggaaattct ctccgcctc gttattgtc aaagacacc gaaatatac atccaccag acacttatc cacgaaaat aaattcgat gttgacacg aacttaatc actttagac gacgacgtc caaatttat ttctctaat agggacatc ttgggatta gatcaacga atcaagacc gaattttcg gaaatgaca agtgggggc aaaaacttg gccgagatt accggaggc tgcgtgggg gcgtttctg ctggtcgca ggtcttgcc tatttcatc accaagaag ttgagagga gagaaacag aatttcgaa cccaacacc gcaggggca tag
- >TC008047 RA
gaggttgta aaattttct gcgaatcgg agccacgta ttgtgtcta aatggctca agaccgccc taaagccat tgatgacag taaaaactc aagacgtaa cggatagtc tccagacag gggtgctct tgtgatcgg tccagacgt tcatctagt cgaagtgaa gcaagtggt gtgctagct atggcagtg ttaaaaata cttttctta ttttcactt cacatttgt gccccaaat tcggttgtt tgcggaccg actgaagat tataattac tcgttggaa gatgagcaa aattcggtt aatttcaca cacgtgcgt gcgaaaaat ttgagcggg aatgtgcag ttgacgtcg cttgaagtt ttgcgaaca gaaatgtta aacaatggc tttgggaaa gtcgaagag accaccaaa gtaacagtc agtgtaaat aaacacatt ggaacaatc atcgaaaat caacaacaa gactcttta ttgtccgaa gttgggaca aaagctccg acagatggt ctggaaccg atcggtttg atggatgtt tttaacgac acatctggc actttgccc gatcagacc actacagaa aaactaatc actgacact ccattaaat tcagagatc actgaaaaa tcccaagat tgggtttca ggtgatttg agtttaatt tcgacggtt cgaagtgaa agaattaat tcagtggat gatgagaga gttgccttg attagggga ctgatttca gagagtgaa gaaattgag aatttgtcc acgttttta gaggttgat gaagaattg ctaaatgaa catgaggat gagaaggac gtggagggg gcgattgag gacgacatt aataatatt ttgacggaa tacgaagac aatgaagaa gaaataaac caaaataaa acagaaact gggtcacaa gcagttaga gaaagcacc acaattttt actcaagag aaagacgaa acggtggct tcaacgttt acaactgaa gaaatttcc tcgactaca caaaatagt gaaaaagaa attttcaaa caaacaaca atttggaag aaattttat ctagatgaa gaaacaacc acgggattt ttcacaact ttttccgaa aatatttct acagaaaaa ctttacgaa acgacacaa aaagaagaa aatgctgta actgcaaca gatataaca agtcaggga aaagaaaca acagagttt gcagtagct gaagatttt aatgcgatg tcaacagca aaagaagaa atttatatg actcctaca gaaagtaca acttataat tttcttatt gcaaaaact gaaaatgaa gaaattaaa attgctgaa gaaactgaa gcatcgaca ccgaattat gaaaacgat ggaggaaga aaatttgaa acgagccga ataaatgaa gaaagtttg tccactgaa atgtttaca gaagaaccc tccgaaaat attacatta cttttaaat aaacttgaa gaaactgaa ctatcgaca tcaacaacc gaactttac acaacaatt tttggaaaa aatactgaa agaaactcg aatattacc gaaactatg gaaattttt acagaaaat caaactgaa attgtacaa aatcaggaa gggaattta gctacaaca ccactttat gagaaggaa ttagaacaa ttagaagaa attattgat aacattaca acaactttg aataaatac gaagaaatt caagttttc acgtatgaa cagcaaaca acacagttt cagcaacag tttgacacc accacagaa catgaagaa agcaagaga aaggcagaa aaagaaaag tttgcaatg aaagaagca gaggaagac caaattaaa aataaaatc tttacaaaa tccccagaa attttaaga gaaggttgg aaaaacaac acggaagac aagcttgaa aatataaat gaaataact aatgaggaa aattttgat aaaggtcac atatcagaa aaattagca actgaaata cgaggtgaa aacaatgcc gaaaaaaaa gaagaaaat attaacaca aatgaagca agtgatgat attttacca actacaaat tttatttta accacaaat aaaacccaa gaagtactt tccacaacc actgaagaa aatgaagat tttgtgtca gtttccact cccactaac acaacagaa accgaaata aacggaggt tttttgaat tcgttaaca tcaggaatt tctagtttc cttggaact tttttcgat tcagcagaa aaacaattc gaggaaaaa aatttcggt gacgaagac atgaaaaat aaatttgag gaagataaa tataacgac atattcaac gacgagact gaagaaacc aaagaagat aataaaatt aatcatttt actaaacta aaagttaaa gaaccgtac agaaattat ttgaatcag tttgaggaa gaaacgaca acgataaac ttaaacttg gaggatttg aattcagcc tcaaaatca cctcatttg ccctcgaaa gccgacagt ttgatcaat tctccagaa gtgccccaa attttgatt aaaatcaac gacgaagaa acaaaagtt gttatcaaa attatcacc aacgactcg acctcgtac atcccctca aacgccctc gtctcattt ccatccccg aacaccgtc aacatcacg tggaaatcg tcatcgttt cgaaacaat tctttacaa tttgttatg ggaaattct ctccgcctc gttattgtc aaagacacc gaaatatac atccaccag acacttatc cacgaaaat aaattcgat gttgacacg aacttaatc actttagac gacgacgtc caaatttat ttctctaat agggacatc ttgggatta gatcaacga atcaagacc gaattttcg gaaatgaca agtgggggc aaaaacttg gccgagatt accggaggc tgcgtgggg gcgtttctg ctggtcgca ggtcttgcc tatttcatc accaagaag ttgagagga gagaaacag aatttcgaa cccaacacc gcaggggca tagttcagt gtgatgcaa accaaagaa actttatct ttgtagaac aatatttac gtaaatcac tttattgta tataaaaac ctcgttctt ttgtaataa atttttata attact
