Status
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Gene information
General information
TC032803
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008042,TC008040,TC008039,TC008038
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4150298..4253288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032803
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04418

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032803 RA
      MSEFGRPLP ARRRATPIP VPPPRIPTP MPPSNNNNP 
      ARSLHSKDP NQRIHPSSV GSDIPKLIT KASYKLLDN 
      DVKTAWINP RLISKIDLE VIKSTEYVP PPEHNMNGH 
      YSHLMSHQN QSCGRSQRE WESAVNGRA KSRSVPAPT 
      AAAADAIIR RRKPSTKPQ MEANTDRLL SNNRGLRSK 
      TKSQRQKVK GVVGYQSAT ACYVGGGSG GGGNDSAVY 
      GVYSETKYT FAVNGMPGT AAQASAAAA FFARAAQKL 
      NLSSSPHRR KRYNSEEAD SSGFCGALQ RSPPPVPPP 
      LLRRIGVRE VTGVGKVKV MLRVSNPPG SGTEACSNT 
      FFSLDKRKK QVNLIDPAT CGGSSAPED RRVGVAAPK 
      MFAFDAIFS QDDSQTEVC SSALTDVIH AVINGNDGC 
      LFCFGHAGL GKSYTMLGT PENANTLGI IPCAISWLF 
      KGINEQKQK TGARFSVRV SALEVCGPT NQMRDLLAG 
      YANDSEQSP GVYLRDDPL FGNQPPHCE LRVPTAEKA 
      AHYLDAAVD CRAPTREES RDTHLLFTL HVYQYSVAG 
      KGGVAGGRS RLHLIDLGN SERGKASGG IPLSGLGNI 
      LLAIFNGQK HLPYKEHKL TQLLKDCLG SLTCHAAMI 
      AHVSPSAQN YSDTLTTVQ LASRIHRMR RRRIKFVSS 
      GTGSGGSSG EEPTRTTGT SSEPDPSSS DLSADTVIY 
      VGPADDATD GEHPPVYIP SLNSGDNRC SMSKVLRGS 
      SAEQRPKVP TKDTHRPAK TSCQSNKTS PAHTNSPKS 
      SPSRAKPLK GTSDEQWID GPRISKSKV AEARHLMKE 
      PCHVKKRET WIDGPMQAE ATGYGYMDS HKKNMIRKW 
      VEHQTSQIQ RTKPKQYKE LTQFKTEDE IVKPLDSKK 
      AETVEESVI RTGLKLNSI KNDAIPECQ TPEEKEPAD 
      DLEDEEEEP ELPPALPLI QPLSSREVS LESLDMLLK 
      ERMLSNAEY QNYQNPESD EDEVLEIIE VEEPLEPVP 
      TQDSCLQVT EEDIALCMG YIENPLPEV DQENPLDHP 
      LRILSQENL TVVSTFTDS MSVYTDLER ILPRHKYDP 
      QYDYEDPDN PYPRNGNFD ESTRKKFDQ LARLHQLYT 
      NRLAKANVA NSETYQKQQ KNRTLSRCE SLSLTDMLY 
      GGGYPDNGS IYSEPAYVQ EKFCENCKI NLGRPATSQ 
      NWYTDLYLS ASTQDLSQK KSDSLGGRF QRYCHNYDH 
      NLAYLRHPD GASNPNLEE SRVHASTSE SAPAPPLPP 
      PNSLPSVER MNRAILSIE AGTAIKLTH KSEGYDSGH 
      DSTPRTSKH SPAAISRRA ESGYDSVVR DSESSSIDS 
      DSMSHLHGR RGKCKHKHE KSFCSWFLN PFTCKYIDD 
      PPETHF
    CDS sequence
    • >TC032803 RA
      atgagcgag ttcgggcgg ccacttccg gcccgccgg 
      cgagccacc cccattccc gtgccgccc ccgagaatt 
      cccacgccg atgccgcca tccaacaac aacaatccc 
      gccagatcc ttgcattcg aaagatccc aaccagcga 
      atccatccc tcgtcagtt gggtccgac attcctaag 
      cttatcaca aaagcttcc tataagcta ctggataac 
      gatgtcaaa acggcatgg atcaatcct cggctaatc 
      tcaaagatc gacttggag gtgataaag tcgacggag 
      tacgtgccg cccccggag cacaacatg aatggccac 
      tacagccac ctcatgtct caccagaac caaagctgc 
      ggaagatcc caaagagag tgggagagt gcggtgaat 
      gggcgcgcg aaatcccga tcagtgcct gccccaaca 
      gcggcggca gcagacgca atcatcaga cggaggaag 
      ccatccacg aagccgcaa atggaagcc aacaccgac 
      cgactgctc agcaacaat cgcggcctc cgatcaaag 
      accaaatcg caaagacaa aaagtgaaa ggggtcgtg 
      gggtaccag agcgcgacc gcgtgctac gtcgggggt 
      ggcagtggc ggcggcggc aatgacagt gcagtgtac 
      ggtgtctac tccgaaact aaatacact tttgcagtg 
      aacggcatg ccggggacg gcggctcag gcctcggcg 
      gcggcggcg tttttcgca agagccgcc caaaagctg 
      aacctctcg tcgtcgccg caccgccgg aaacgctac 
      aactctgaa gaagccgac tcctccggt ttctgcggc 
      gctttgcag cggtcgccg ccccccgtg ccgccgccc 
      cttctgcga agaatcgga gtcagagaa gtcaccgga 
      gtcggcaag gtgaaggtg atgttgagg gtgtccaac 
      ccgcccggc tccggtacg gaggcgtgt tcgaacacg 
      ttcttcagc ttggacaag cggaagaag caagtgaat 
      ttgatcgac ccggccact tgtggaggc tcgtcggca 
      cctgaagac cgacgagtg ggcgtcgcg gcgcccaaa 
      atgttcgcc ttcgacgcg attttctcg caggacgac 
      tcgcagacg gaggtgtgt tcgagtgcc ctcacggac 
      gtgatccat gctgttatc aacggcaat gatggctgt 
      ctgttctgt tttgggcat gcaggactt ggcaagtcg 
      tatacgatg ctgggaacc cccgaaaac gccaacact 
      ctgggcata atcccctgt gccatttcg tggctgttc 
      aaaggaatc aacgaacag aaacaaaag accggcgcc 
      cgcttttcc gtccgcgtg tcagccctg gaagtgtgc 
      ggccccacg aaccaaatg cgggacctc cttgcaggc 
      tacgccaac gactcggag caatccccc ggcgtttac 
      ctccgcgac gacccttta tttggcaac cagcccccg 
      cattgtgag ctgcgggtg ccgactgcg gagaaagcg 
      gcccactac ttggacgcc gccgtcgac tgtcgagca 
      cccactcga gaggagtcc cgagacacc cacctcctg 
      ttcaccctc cacgtgtat cagtacagc gtcgcggga 
      aaaggcgga gtcgccggt ggaagaagc cgcctgcat 
      ctgatcgat ctcggcaat tccgaacgc gggaaggcc 
      agcggaggc atcccctta tcgggcttg gggaacatc 
      ctccttgcg attttcaac ggccagaag cacctcccg 
      tacaaggaa cacaagctg acccaactc ctaaaagac 
      tgtctcggg tccctgact tgccatgca gccatgata 
      gcgcatgtt tcgccctca gcccagaat tactccgat 
      actttgacc acggtccag ctagcatcg aggatacac 
      cgaatgcgg aggcggagg atcaagttc gtgtcgtct 
      ggaaccggc tccggtgga agttccggg gaggaaccg 
      acccggacc accggaacc agcagcgaa cctgaccct 
      tcctccagc gacctaagt gctgatact gtcatttac 
      gtcggtccg gctgacgac gcaaccgac ggggaacac 
      ccccctgta tatatccct agccttaat tccggagac 
      aatcgatgc tcgatgagc aaagttctg cgaggttcg 
      agtgcagaa caacgacca aaagttcct accaaagac 
      acacatcgt ccggcgaaa acttcgtgc caaagcaac 
      aaaacatca ccagctcac acaaactca cccaaatca 
      tcaccaagt cgagccaaa ccgctcaaa ggaaccagc 
      gacgagcag tggatcgac ggaccaaga atctcaaag 
      tcgaaagta gccgaagcc aggcatttg atgaaagaa 
      ccgtgccat gtcaaaaag cgcgaaacg tggatcgat 
      gggcccatg caagccgaa gccaccggc tacggctac 
      atggacagc cacaagaaa aacatgatc aggaaatgg 
      gtggaacac caaacgtcc caaatacag agaactaag 
      cccaaacag tacaaagaa ctgacgcag tttaagaca 
      gaagacgaa atcgtcaaa cccctagac agcaaaaag 
      gctgaaacg gtcgaagaa tcggtgatc agaacggga 
      ctcaagcta aactccatc aaaaacgac gcaatcccc 
      gaatgccaa accccggaa gagaaagaa cctgcagac 
      gacctggaa gacgaagaa gaggaacca gagctacca 
      ccggcactt cccttaata cagcccttg agcagccga 
      gaagtgtcc ctcgaaagt ctcgacatg ctgctaaag 
      gagcgcatg ctgtcgaac gccgagtac caaaattac 
      caaaacccg gaaagcgac gaagatgaa gtcttggaa 
      attattgag gtggaggag cccctcgag ccggtcccg 
      acccaggac agctgcctc caggtgaca gaagaggac 
      atagccctc tgtatgggc tacatcgag aacccgctc 
      ccggaggtg gaccaagag aaccctctc gaccaccct 
      ttgcggatc ttaagccag gagaattta acagttgtg 
      tcgactttc accgactca atgtcagtc tacactgac 
      cttgagcgg atccttccg agacacaag tacgaccct 
      cagtacgat tacgaagac cccgataat ccctatcca 
      agaaacggc aatttcgac gaatcgact cggaaaaag 
      ttcgaccag ttggcacgc ctgcaccaa ttgtacacg 
      aaccgactg gccaaagcc aacgtcgcc aattcggaa 
      acgtaccaa aagcagcaa aaaaaccgc acgctgagc 
      cggtgtgag tcactatca ctaaccgac atgttgtac 
      ggtggcggc taccctgac aatgggagt atctactcg 
      gagccggcc tacgtgcag gagaaattt tgcgaaaat 
      tgtaaaata aatttaggc cgtccggcc acgtcccag 
      aactggtac accgacttg tacttgtcg gccagcacc 
      caggacttg tcccagaaa aagagcgac tctttaggt 
      ggccgcttc cagcgctac tgccacaac tacgaccac 
      aacctggcc tacttgcgt caccccgac ggggcctcc 
      aatccgaat ttagaagaa tccagggtc cacgcttcg 
      acgtccgaa agcgccccc gcgccgccg ctgccgccc 
      ccgaacagt cttccctcc gtcgaacgc atgaacagg 
      gccattctc agcatcgag gccgggact gcgatcaaa 
      ctgacccac aagtcggaa gggtacgac agtggacac 
      gactccacc cccaggacg tccaagcac agtccggcg 
      gccatcagc cgaagggcc gagagcggc tacgacagt 
      gtggtgagg gacagcgaa agttcgagc atcgactcg 
      gacagcatg tcgcacttg catggccgc aggggcaag 
      tgcaagcac aagcacgag aagagcttc tgttcgtgg 
      tttcttaac ccgttcacg tgtaaatat atcgacgac 
      ccgcccgaa acgcatttc taa
    mRNA sequence
    • >TC032803 RA
      gagttccat tccatgtgc caccgacca gtaaacaat 
      acggttgcg ccctttcga aacgccggt cgaggagag 
      ctcttgcag tcggtgcgt tcgtcttgc acatgtgag 
      acatgtgtt cgggtgcac attcatctc tcgtcgaga 
      ctaattgtg acgaaatgt gattttttt cgaaatacg 
      tgttcgagt gattcgtaa ttggagaga attagtgca 
      ggtgacgga gggttaaag gcttaaagc tccacggag 
      gaaagatga gcgagttcg ggcggccac ttccggccc 
      gccggcgag ccaccccca ttcccgtgc cgcccccga 
      gaattccca cgccgatgc cgccatcca acaacaaca 
      atcccgcca gatccttgc attcgaaag atcccaacc 
      agcgaatcc atccctcgt cagttgggt ccgacattc 
      ctaagctta tcacaaaag cttcctata agctactgg 
      ataacgatg tcaaaacgg catggatca atcctcggc 
      taatctcaa agatcgact tggaggtga taaagtcga 
      cggagtacg tgccgcccc cggagcaca acatgaatg 
      gccactaca gccacctca tgtctcacc agaaccaaa 
      gctgcggaa gatcccaaa gagagtggg agagtgcgg 
      tgaatgggc gcgcgaaat cccgatcag tgcctgccc 
      caacagcgg cggcagcag acgcaatca tcagacgga 
      ggaagccat ccacgaagc cgcaaatgg aagccaaca 
      ccgaccgac tgctcagca acaatcgcg gcctccgat 
      caaagacca aatcgcaaa gacaaaaag tgaaagggg 
      tcgtggggt accagagcg cgaccgcgt gctacgtcg 
      ggggtggca gtggcggcg gcggcaatg acagtgcag 
      tgtacggtg tctactccg aaactaaat acacttttg 
      cagtgaacg gcatgccgg ggacggcgg ctcaggcct 
      cggcggcgg cggcgtttt tcgcaagag ccgcccaaa 
      agctgaacc tctcgtcgt cgccgcacc gccggaaac 
      gctacaact ctgaagaag ccgactcct ccggtttct 
      gcggcgctt tgcagcggt cgccgcccc ccgtgccgc 
      cgccccttc tgcgaagaa tcggagtca gagaagtca 
      ccggagtcg gcaaggtga aggtgatgt tgagggtgt 
      ccaacccgc ccggctccg gtacggagg cgtgttcga 
      acacgttct tcagcttgg acaagcgga agaagcaag 
      tgaatttga tcgacccgg ccacttgtg gaggctcgt 
      cggcacctg aagaccgac gagtgggcg tcgcggcgc 
      ccaaaatgt tcgccttcg acgcgattt tctcgcagg 
      acgactcgc agacggagg tgtgttcga gtgccctca 
      cggacgtga tccatgctg ttatcaacg gcaatgatg 
      gctgtctgt tctgttttg ggcatgcag gacttggca 
      agtcgtata cgatgctgg gaacccccg aaaacgcca 
      acactctgg gcataatcc cctgtgcca tttcgtggc 
      tgttcaaag gaatcaacg aacagaaac aaaagaccg 
      gcgcccgct tttccgtcc gcgtgtcag ccctggaag 
      tgtgcggcc ccacgaacc aaatgcggg acctccttg 
      caggctacg ccaacgact cggagcaat cccccggcg 
      tttacctcc gcgacgacc ctttatttg gcaaccagc 
      ccccgcatt gtgagctgc gggtgccga ctgcggaga 
      aagcggccc actacttgg acgccgccg tcgactgtc 
      gagcaccca ctcgagagg agtcccgag acacccacc 
      tcctgttca ccctccacg tgtatcagt acagcgtcg 
      cgggaaaag gcggagtcg ccggtggaa gaagccgcc 
      tgcatctga tcgatctcg gcaattccg aacgcggga 
      aggccagcg gaggcatcc ccttatcgg gcttgggga 
      acatcctcc ttgcgattt tcaacggcc agaagcacc 
      tcccgtaca aggaacaca agctgaccc aactcctaa 
      aagactgtc tcgggtccc tgacttgcc atgcagcca 
      tgatagcgc atgtttcgc cctcagccc agaattact 
      ccgatactt tgaccacgg tccagctag catcgagga 
      tacaccgaa tgcggaggc ggaggatca agttcgtgt 
      cgtctggaa ccggctccg gtggaagtt ccggggagg 
      aaccgaccc ggaccaccg gaaccagca gcgaacctg 
      acccttcct ccagcgacc taagtgctg atactgtca 
      tttacgtcg gtccggctg acgacgcaa ccgacgggg 
      aacaccccc ctgtatata tccctagcc ttaattccg 
      gagacaatc gatgctcga tgagcaaag ttctgcgag 
      gttcgagtg cagaacaac gaccaaaag ttcctacca 
      aagacacac atcgtccgg cgaaaactt cgtgccaaa 
      gcaacaaaa catcaccag ctcacacaa actcaccca 
      aatcatcac caagtcgag ccaaaccgc tcaaaggaa 
      ccagcgacg agcagtgga tcgacggac caagaatct 
      caaagtcga aagtagccg aagccaggc atttgatga 
      aagaaccgt gccatgtca aaaagcgcg aaacgtgga 
      tcgatgggc ccatgcaag ccgaagcca ccggctacg 
      gctacatgg acagccaca agaaaaaca tgatcagga 
      aatgggtgg aacaccaaa cgtcccaaa tacagagaa 
      ctaagccca aacagtaca aagaactga cgcagttta 
      agacagaag acgaaatcg tcaaacccc tagacagca 
      aaaaggctg aaacggtcg aagaatcgg tgatcagaa 
      cgggactca agctaaact ccatcaaaa acgacgcaa 
      tccccgaat gccaaaccc cggaagaga aagaacctg 
      cagacgacc tggaagacg aagaagagg aaccagagc 
      taccaccgg cacttccct taatacagc ccttgagca 
      gccgagaag tgtccctcg aaagtctcg acatgctgc 
      taaaggagc gcatgctgt cgaacgccg agtaccaaa 
      attaccaaa acccggaaa gcgacgaag atgaagtct 
      tggaaatta ttgaggtgg aggagcccc tcgagccgg 
      tcccgaccc aggacagct gcctccagg tgacagaag 
      aggacatag ccctctgta tgggctaca tcgagaacc 
      cgctcccgg aggtggacc aagagaacc ctctcgacc 
      accctttgc ggatcttaa gccaggaga atttaacag 
      ttgtgtcga ctttcaccg actcaatgt cagtctaca 
      ctgaccttg agcggatcc ttccgagac acaagtacg 
      accctcagt acgattacg aagaccccg ataatccct 
      atccaagaa acggcaatt tcgacgaat cgactcgga 
      aaaagttcg accagttgg cacgcctgc accaattgt 
      acacgaacc gactggcca aagccaacg tcgccaatt 
      cggaaacgt accaaaagc agcaaaaaa accgcacgc 
      tgagccggt gtgagtcac tatcactaa ccgacatgt 
      tgtacggtg gcggctacc ctgacaatg ggagtatct 
      actcggagc cggcctacg tgcaggaga aattttgcg 
      aaaattgta aaataaatt taggccgtc cggccacgt 
      cccagaact ggtacaccg acttgtact tgtcggcca 
      gcacccagg acttgtccc agaaaaaga gcgactctt 
      taggtggcc gcttccagc gctactgcc acaactacg 
      accacaacc tggcctact tgcgtcacc ccgacgggg 
      cctccaatc cgaatttag aagaatcca gggtccacg 
      cttcgacgt ccgaaagcg cccccgcgc cgccgctgc 
      cgcccccga acagtcttc cctccgtcg aacgcatga 
      acagggcca ttctcagca tcgaggccg ggactgcga 
      tcaaactga cccacaagt cggaagggt acgacagtg 
      gacacgact ccaccccca ggacgtcca agcacagtc 
      cggcggcca tcagccgaa gggccgaga gcggctacg 
      acagtgtgg tgagggaca gcgaaagtt cgagcatcg 
      actcggaca gcatgtcgc acttgcatg gccgcaggg 
      gcaagtgca agcacaagc acgagaaga gcttctgtt 
      cgtggtttc ttaacccgt tcacgtgta aatatatcg 
      acgacccgc ccgaaacgc atttctaac ttggggact 
      tccccgacg caagactga tttttacaa gggatgcgc 
      tctagttgg ccgcgattt tttcctttc aactgtact 
      gaaagcagg acatttgcc gtggccaac tgtgtgccc 
      gattccttc gtatataaa caccgactt ttcatttcc 
      gatgcatcg caaaattcc gtaccgtcg ttggccgtg 
      gtaactcaa taacgcaac gaaacagta tgaacctgt 
      atacgtcga aatgtgtca aatttacct ttaggatca 
      tttatatca ctttaaatg tgcataatt tgaggatat 
      tagacgaaa acttggcat gtacatcta tttttggta 
      tcgatattg tttacttcc tgcatatca ttagaagtt 
      cgagggatc aaaatgata tcttgccaa ctgtttcta 
      aacgaaaac tagcttaag actgcttgt caattttga 
      tctcgacat ctaaatttt atacaaggc ttcttgata 
      cgactaaaa tgaatttta tttgtgata aattgtaag 
      tagtagtga acgcctttc cgtttgttc gaatttata 
      taaactgta aattagtgt aaatttgtg tatttattt 
      caattttat actgttatt aataatcat ttttgaatt 
      ta
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