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Gene information
General information
TC007475 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3936047..3942857
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007475
    iBeetle screen iB_04416

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007475 RA
      MYGFVNYAL ELLVVKTFG EETWETIKK NADLQMEGS 
      FLVRQIYED ELTYNLISA AVDVLQIPA NAILELFGK 
      TFFEFCQDS GFDKILQVL GATPRDFLQ NLDALHDHL 
      GTLYPGMKA PSFRCTVRP EDGALILHY YSDRPGLEH 
      IVIGIVKTV ASKLHGTEV VVDILKTKE ECDHVQFLI 
      TEASGPGKV QHPEMDEIQ TLSLEPKVN AATFCRVFP 
      FHIMFDRDL KIVQTGSTV ARVIPKVTS AECRVTDIL 
      DTVRPHLEL TFENILSHI NTIYVLKTR PGVMQVCAP 
      PEYRYLRLK GQMLYVPET DLVIFLCYP SVMNLDDLT 
      RRGLYISDI PLHDATRDL VLMSEQFEA DYKLTRNLE 
      LLTDKLQQT YRELDREKK KTDGLLYSV LPISVANEL 
      RHKRPVPAK RYDCVTLLF SGIVNFSAL CAANTDSKG 
      VMKIVNMLN ELYTNFDVL TDPKKNPNI YKVETVGDK 
      YMAVSGLPE PCTTHACNI ARLALDMMD RGKSVMFDG 
      KPVKITIGI HSGEVVTGV IGHRMPRYC LFGNTVNLT 
      SRTETTGEP GRINVSEEA YKFLCKEDN FDEQFHFEY 
      RGPVTMKGK SEPMNVWFL SRAKSH
    • >TC007475 RB
      MYGFVNYAL ELLVVKTFG EETWETIKK NADLQMEGS 
      FLVRQIYED ELTYNLISA AVDVLQIPA NAILELFGK 
      TFFEFCQDS GFDKILQVL GATPRDFLQ NLDALHDHL 
      GTLYPGMKA PSFRCTVRP EDGALILHY YSDRPGLEH 
      IVIGIVKTV ASKLHGTEV VVDILKTKE ECDHVQFLI 
      TEASGPGKV QHPEMDEIQ TLSLEPKVN AATFCRVFP 
      FHIMFDRDL KIVQTGSTV ARVIPKVTS AECRVTDIL 
      DTVRPHLEL TFENILSHI NTIYVLKTR PGVMQVCAP 
      PEYRYLRLK GQMLYVPET DLVIFLCYP SVMNLDDLT 
      RRGLYISDI PLHDATRDL VLMSEQFEA DYKLTRNLE 
      LLTDKLQQT YRELDREKK KTDGLLYSV LPISVANEL 
      RHKRPVPAK RYDCVTLLF SGIVNFSAL CAANTDSKG 
      VMKIVNMLN ELYTNFDVL TDPKKNPNI YKVETVGDK 
      YMAVSGLPE PCTTHACNI ARLALDMMD RGKSVMFDG 
      KPVKITIGI HSGEVVTGV IGHRMPRYC LFGNTVNLT 
      SRTETTGEP GRINVSEEA YKESGTERV RIPDGSWQF 
      SQSWFRIVQ RVKCFSSGT WTPQRVKGI KPSSTKSG
    CDS sequence
    • >TC007475 RA
      atgtacggg tttgtgaac tatgctcta gaacttctc 
      gtcgttaag acttttggc gaggaaact tgggagacg 
      atcaaaaaa aatgccgac ctccaaatg gaaggctcg 
      ttcctcgtc cggcaaatc tacgaggac gagctcaca 
      tataacttg atttcagca gcggttgat gttttgcaa 
      ataccagca aatgcaatt ttggaactc ttcggcaaa 
      acttttttc gagttttgc caagattcc ggctttgat 
      aagatcctt caagtgttg ggcgccact cctagggac 
      tttctccag aatctggat gctctccac gaccattta 
      ggcacccta tatccgggg atgaaagct ccgtctttc 
      cggtgcacc gttcggcct gaagatggc gctttaatc 
      ctgcactac tactcagac aggcccgga ctcgagcat 
      attgtaatc gggatagtc aagaccgtt gcaagtaaa 
      ttacacggg actgaagtt gtagttgac atcctcaaa 
      accaaggaa gaatgcgat cacgtgcaa tttttgata 
      accgaagct tcgggtccg ggcaaagtc cagcatccg 
      gaaatggac gagatccaa acattatca ctcgagccc 
      aaggtgaac gccgcgaca ttttgccgc gtgtttcct 
      ttccacatc atgttcgac cgagacctg aagatcgtc 
      caaacaggt tcaacggtg gcccgtgtc atcccaaag 
      gtgacctcc gccgagtgc cgagtcacc gacatcctg 
      gacacggtg cgtccccac ctggagctg accttcgag 
      aacatcctc tcgcacata aacaccatc tacgtcctc 
      aagacccgg cccggggtg atgcaagtc tgcgcgccc 
      cccgagtat cgctacctc cgtctcaaa ggccaaatg 
      ctctacgtg ccggagacc gacttggtg atcttcctc 
      tgctacccc agcgtgatg aatctggac gaccttacg 
      aggaggggg ctctacatc agcgacatt cctctacat 
      gacgccaca cgcgatctg gtactcatg tccgagcaa 
      ttcgaggct gattacaag ttgacgagg aacttggaa 
      ctgctcacg gataagttg cagcagacg tacagggag 
      ctggacagg gagaagaag aagacggac gggttgttg 
      tactcggtg ctgcccatc agtgtggca aatgagctg 
      agacacaag agaccagtg ccggccaag aggtacgac 
      tgcgtcacg ctgcttttt tcaggaatt gttaacttc 
      tccgccctg tgtgcggcc aataccgac tccaaagga 
      gtcatgaag atcgtcaac atgctgaac gagctctac 
      acgaatttt gatgtcttg acggatccc aaaaagaat 
      ccaaatatt tacaaggtc gagactgtt ggcgacaaa 
      tacatggcg gtgagcggc ctcccggag ccctgcacc 
      actcacgct tgcaacatc gcccgttta gccctcgac 
      atgatggac cgcggcaag tcggtcatg ttcgatggc 
      aagcctgtg aaaatcaca attgggata cattctggt 
      gaggttgtg acaggggtc ataggccac cgcatgccc 
      cgatattgc ctttttggc aacacggtc aacttgaca 
      agtcgcacg gaaacaacc ggcgaacct ggaaggatc 
      aacgtttcg gaagaggcg tacaagttc ctgtgcaag 
      gaggacaat ttcgatgag cagttccat ttcgagtat 
      cgcgggccg gtgaccatg aagggcaag tctgaaccc 
      atgaacgtg tggttcttg tcgcgagcc aagtcccac 
      tag
    • >TC007475 RB
      atgtacggg tttgtgaac tatgctcta gaacttctc 
      gtcgttaag acttttggc gaggaaact tgggagacg 
      atcaaaaaa aatgccgac ctccaaatg gaaggctcg 
      ttcctcgtc cggcaaatc tacgaggac gagctcaca 
      tataacttg atttcagca gcggttgat gttttgcaa 
      ataccagca aatgcaatt ttggaactc ttcggcaaa 
      acttttttc gagttttgc caagattcc ggctttgat 
      aagatcctt caagtgttg ggcgccact cctagggac 
      tttctccag aatctggat gctctccac gaccattta 
      ggcacccta tatccgggg atgaaagct ccgtctttc 
      cggtgcacc gttcggcct gaagatggc gctttaatc 
      ctgcactac tactcagac aggcccgga ctcgagcat 
      attgtaatc gggatagtc aagaccgtt gcaagtaaa 
      ttacacggg actgaagtt gtagttgac atcctcaaa 
      accaaggaa gaatgcgat cacgtgcaa tttttgata 
      accgaagct tcgggtccg ggcaaagtc cagcatccg 
      gaaatggac gagatccaa acattatca ctcgagccc 
      aaggtgaac gccgcgaca ttttgccgc gtgtttcct 
      ttccacatc atgttcgac cgagacctg aagatcgtc 
      caaacaggt tcaacggtg gcccgtgtc atcccaaag 
      gtgacctcc gccgagtgc cgagtcacc gacatcctg 
      gacacggtg cgtccccac ctggagctg accttcgag 
      aacatcctc tcgcacata aacaccatc tacgtcctc 
      aagacccgg cccggggtg atgcaagtc tgcgcgccc 
      cccgagtat cgctacctc cgtctcaaa ggccaaatg 
      ctctacgtg ccggagacc gacttggtg atcttcctc 
      tgctacccc agcgtgatg aatctggac gaccttacg 
      aggaggggg ctctacatc agcgacatt cctctacat 
      gacgccaca cgcgatctg gtactcatg tccgagcaa 
      ttcgaggct gattacaag ttgacgagg aacttggaa 
      ctgctcacg gataagttg cagcagacg tacagggag 
      ctggacagg gagaagaag aagacggac gggttgttg 
      tactcggtg ctgcccatc agtgtggca aatgagctg 
      agacacaag agaccagtg ccggccaag aggtacgac 
      tgcgtcacg ctgcttttt tcaggaatt gttaacttc 
      tccgccctg tgtgcggcc aataccgac tccaaagga 
      gtcatgaag atcgtcaac atgctgaac gagctctac 
      acgaatttt gatgtcttg acggatccc aaaaagaat 
      ccaaatatt tacaaggtc gagactgtt ggcgacaaa 
      tacatggcg gtgagcggc ctcccggag ccctgcacc 
      actcacgct tgcaacatc gcccgttta gccctcgac 
      atgatggac cgcggcaag tcggtcatg ttcgatggc 
      aagcctgtg aaaatcaca attgggata cattctggt 
      gaggttgtg acaggggtc ataggccac cgcatgccc 
      cgatattgc ctttttggc aacacggtc aacttgaca 
      agtcgcacg gaaacaacc ggcgaacct ggaaggatc 
      aacgtttcg gaagaggcg tacaaggag tcgggcact 
      gagagagtc cggattccg gacgggagc tggcagttc 
      agccagagt tggttccgg atagtgcag cgggtcaag 
      tgtttcagt tccgggact tggacgcca cagcgcgtc 
      aagggaatt aaaccgtct tccaccaaa tccggataa
    mRNA sequence
    • >TC007475 RA
      tggctgttt tcagctttt tcagtgctt tcagcgccg 
      gcaaacacg caacagtgc caacaagaa cgacttgct 
      ggtgcgcga ccatcatgt acgggtttg tgaactatg 
      ctctagaac ttctcgtcg ttaagactt ttggcgagg 
      aaacttggg agacgatca aaaaaaatg ccgacctcc 
      aaatggaag gctcgttcc tcgtccggc aaatctacg 
      aggacgagc tcacatata acttgattt cagcagcgg 
      ttgatgttt tgcaaatac cagcaaatg caattttgg 
      aactcttcg gcaaaactt ttttcgagt tttgccaag 
      attccggct ttgataaga tccttcaag tgttgggcg 
      ccactccta gggactttc tccagaatc tggatgctc 
      tccacgacc atttaggca ccctatatc cggggatga 
      aagctccgt ctttccggt gcaccgttc ggcctgaag 
      atggcgctt taatcctgc actactact cagacaggc 
      ccggactcg agcatattg taatcggga tagtcaaga 
      ccgttgcaa gtaaattac acgggactg aagttgtag 
      ttgacatcc tcaaaacca aggaagaat gcgatcacg 
      tgcaatttt tgataaccg aagcttcgg gtccgggca 
      aagtccagc atccggaaa tggacgaga tccaaacat 
      tatcactcg agcccaagg tgaacgccg cgacatttt 
      gccgcgtgt ttcctttcc acatcatgt tcgaccgag 
      acctgaaga tcgtccaaa caggttcaa cggtggccc 
      gtgtcatcc caaaggtga cctccgccg agtgccgag 
      tcaccgaca tcctggaca cggtgcgtc cccacctgg 
      agctgacct tcgagaaca tcctctcgc acataaaca 
      ccatctacg tcctcaaga cccggcccg gggtgatgc 
      aagtctgcg cgccccccg agtatcgct acctccgtc 
      tcaaaggcc aaatgctct acgtgccgg agaccgact 
      tggtgatct tcctctgct accccagcg tgatgaatc 
      tggacgacc ttacgagga gggggctct acatcagcg 
      acattcctc tacatgacg ccacacgcg atctggtac 
      tcatgtccg agcaattcg aggctgatt acaagttga 
      cgaggaact tggaactgc tcacggata agttgcagc 
      agacgtaca gggagctgg acagggaga agaagaaga 
      cggacgggt tgttgtact cggtgctgc ccatcagtg 
      tggcaaatg agctgagac acaagagac cagtgccgg 
      ccaagaggt acgactgcg tcacgctgc ttttttcag 
      gaattgtta acttctccg ccctgtgtg cggccaata 
      ccgactcca aaggagtca tgaagatcg tcaacatgc 
      tgaacgagc tctacacga attttgatg tcttgacgg 
      atcccaaaa agaatccaa atatttaca aggtcgaga 
      ctgttggcg acaaataca tggcggtga gcggcctcc 
      cggagccct gcaccactc acgcttgca acatcgccc 
      gtttagccc tcgacatga tggaccgcg gcaagtcgg 
      tcatgttcg atggcaagc ctgtgaaaa tcacaattg 
      ggatacatt ctggtgagg ttgtgacag gggtcatag 
      gccaccgca tgccccgat attgccttt ttggcaaca 
      cggtcaact tgacaagtc gcacggaaa caaccggcg 
      aacctggaa ggatcaacg tttcggaag aggcgtaca 
      agttcctgt gcaaggagg acaatttcg atgagcagt 
      tccatttcg agtatcgcg ggccggtga ccatgaagg 
      gcaagtctg aacccatga acgtgtggt tcttgtcgc 
      gagccaagt cccactaga gctaacaat ccaactagg 
      tcagtatgg taattatgt tcggcacat gctttcttc 
      attttcgtg agagacgat gtggtttca tcttttgta 
      attgctcgt gtgaaatta cacgttccc tagagacaa 
      gtggagcaa ttgttgatc aaaaatcgg gtaattgta 
      ggacatggt aattacgag agcgcttgc gtaagcgaa 
      tgaaagttg tgcgtcgat gggccctaa acttttcct 
      gcaacctaa cagcatatt tttgttcca ggctgagcg 
      gcgtcgtgc acactcttt aagtaccat tccatgtca 
      gcgcgtgac aatctattc aagcgtatt gtttcagtt 
      atgacatcg cattctcgt gtgtaatta tccccgaca 
      agctacact tataaatat ctccgatag ataattttt 
      aatcggttg tgtcagcac gcgtaatag aaaatccat 
      gtcgactgt catcgctgt ctcttcagt gtgctttcc 
      tttccacca ccgacataa cggccgaat tttactcga 
      gccgcgccc acactttgc ccaaaattc gttcacctg 
      tagagccac cttgaaggc aaggtgaac cgcctcatt 
      ccacagcca cttgtcgcc tgcacagac gtaaacgcg 
      accgttagg acacgtgag caggcttca ggaccaaga 
      aatgtgcat tttggtcgg ccttgaacc gcaaggaac 
      gacaaacgg acacatttt atgcatctt tgaggtgtt 
      tccgggtga aatgtcaac gctaggcga aatttatgg 
      ccttggaag gaacgtttc gccattgtt tactaatat 
      tagcacgtt tccagttgt ttctcttgt ttgtgaaat 
      ggtaaacaa caataaatc aattttagg taaatctac 
      cattttcac acctgtaag tggccaatt aagagtgta 
      ggaaaggtc agagattgt cttttgttt tgttttcaa 
      aagtaataa acgtttacc aagaaa
    • >TC007475 RB
      tggctgttt tcagctttt tcagtgctt tcagcgccg 
      gcaaacacg caacagtgc caacaagaa cgacttgct 
      ggtgcgcga ccatcatgt acgggtttg tgaactatg 
      ctctagaac ttctcgtcg ttaagactt ttggcgagg 
      aaacttggg agacgatca aaaaaaatg ccgacctcc 
      aaatggaag gctcgttcc tcgtccggc aaatctacg 
      aggacgagc tcacatata acttgattt cagcagcgg 
      ttgatgttt tgcaaatac cagcaaatg caattttgg 
      aactcttcg gcaaaactt ttttcgagt tttgccaag 
      attccggct ttgataaga tccttcaag tgttgggcg 
      ccactccta gggactttc tccagaatc tggatgctc 
      tccacgacc atttaggca ccctatatc cggggatga 
      aagctccgt ctttccggt gcaccgttc ggcctgaag 
      atggcgctt taatcctgc actactact cagacaggc 
      ccggactcg agcatattg taatcggga tagtcaaga 
      ccgttgcaa gtaaattac acgggactg aagttgtag 
      ttgacatcc tcaaaacca aggaagaat gcgatcacg 
      tgcaatttt tgataaccg aagcttcgg gtccgggca 
      aagtccagc atccggaaa tggacgaga tccaaacat 
      tatcactcg agcccaagg tgaacgccg cgacatttt 
      gccgcgtgt ttcctttcc acatcatgt tcgaccgag 
      acctgaaga tcgtccaaa caggttcaa cggtggccc 
      gtgtcatcc caaaggtga cctccgccg agtgccgag 
      tcaccgaca tcctggaca cggtgcgtc cccacctgg 
      agctgacct tcgagaaca tcctctcgc acataaaca 
      ccatctacg tcctcaaga cccggcccg gggtgatgc 
      aagtctgcg cgccccccg agtatcgct acctccgtc 
      tcaaaggcc aaatgctct acgtgccgg agaccgact 
      tggtgatct tcctctgct accccagcg tgatgaatc 
      tggacgacc ttacgagga gggggctct acatcagcg 
      acattcctc tacatgacg ccacacgcg atctggtac 
      tcatgtccg agcaattcg aggctgatt acaagttga 
      cgaggaact tggaactgc tcacggata agttgcagc 
      agacgtaca gggagctgg acagggaga agaagaaga 
      cggacgggt tgttgtact cggtgctgc ccatcagtg 
      tggcaaatg agctgagac acaagagac cagtgccgg 
      ccaagaggt acgactgcg tcacgctgc ttttttcag 
      gaattgtta acttctccg ccctgtgtg cggccaata 
      ccgactcca aaggagtca tgaagatcg tcaacatgc 
      tgaacgagc tctacacga attttgatg tcttgacgg 
      atcccaaaa agaatccaa atatttaca aggtcgaga 
      ctgttggcg acaaataca tggcggtga gcggcctcc 
      cggagccct gcaccactc acgcttgca acatcgccc 
      gtttagccc tcgacatga tggaccgcg gcaagtcgg 
      tcatgttcg atggcaagc ctgtgaaaa tcacaattg 
      ggatacatt ctggtgagg ttgtgacag gggtcatag 
      gccaccgca tgccccgat attgccttt ttggcaaca 
      cggtcaact tgacaagtc gcacggaaa caaccggcg 
      aacctggaa ggatcaacg tttcggaag aggcgtaca 
      aggagtcgg gcactgaga gagtccgga ttccggacg 
      ggagctggc agttcagcc agagttggt tccggatag 
      tgcagcggg tcaagtgtt tcagttccg ggacttgga 
      cgccacagc gcgtcaagg gaattaaac cgtcttcca 
      ccaaatccg gataatcct caaaaattt ttttggttt 
      ctaaattga tttttggta ccactctga gaagaagtt 
      ggaggcagg ctacaatat tgtaagggt agacgtggc 
      aaaactcca ttagttttt tcatgataa agacgacca 
      aacaatcct cggtcttcg ccccatatc ttaggaaaa 
      tatggagct tgtcaggga gccccaatt gaagggcta 
      ttttagccg gggtcgggt tatcactcc cgcaacttt 
      ccaatggga cgtctataa acctcgtag agtctaatt 
      ccagtcctc ctgtggtcg catggtcga taaaataca 
      caacaaaaa ttagatttt cttcctgac tatcgaaat 
      cgctcttcc agcccgcaa tataataag gggccctgt 
      taccaaaaa tggccccaa tgaaaatcc tttcgtagg 
      ttttctcac aagcgtaga tgctctcca acagggcta 
      aaaccgtcc ctgtatctt tcaactgcc agtcgtagc 
      agactttaa cagcgacgt acaaaatgt tatggtacc 
      gacccatca tacagacgg aaccattgg ccgcccggt 
      tcccttcat ggtacctca cgatggcca taatgcttt 
      cacggacgg ccgattcgg gttcggtgc cggtaccta 
      aagctagtt ttttgcccg tggtttata caaaaaatc 
      ctcacctct ggcatagcc ttcaataag ggtactgat 
      ataagtgac catcatttg gcgctttcg cctgtcatg 
      gaggctcgt tctgggcga gttcgtaaa ctggtcgag 
      gtagcattc gagaaggta gttcatgac actgacagc 
      cctcgacca aaatgctag gaaacattt tattggatt 
      aacttgcgg ggtttcggc ttaagtgca ctttgcagg 
      ttcctgtgc aaggaggac aatttcgat gagcagttc 
      catttcgag tatcgcggg ccggtgacc atgaagggc 
      aagtctgaa cccatgaac gtgtggttc ttgtcgcga 
      gccaagtcc cactagagc taacaatcc aactaggtc 
      agtatggta attatgttc ggcacatgc tttcttcat 
      tttcgtgag agacgatgt ggtttcatc ttttgtaat 
      tgctcgtgt gaaattaca cgttcccta gagacaagt 
      ggagcaatt gttgatcaa aaatcgggt aattgtagg 
      acatggtaa ttacgagag cgcttgcgt aagcgaatg 
      aaagttgtg cgtcgatgg gccctaaac ttttcctgc 
      aacctaaca gcatatttt tgttccagg ctgagcggc 
      gtcgtgcac actctttaa gtaccattc catgtcagc 
      gcgtgacaa tctattcaa gcgtattgt ttcagttat 
      gacatcgca ttctcgtgt gtaattatc cccgacaag 
      ctacactta taaatatct ccgatagat aatttttaa 
      tcggttgtg tcagcacgc gtaatagaa aatccatgt 
      cgactgtca tcgctgtct cttcagtgt gctttcctt 
      tccaccacc gacataacg gccgaattt tactcgagc 
      cgcgcccac actttgccc aaaattcgt tcacctgta 
      gagccacct tgaaggcaa ggtgaaccg cctcattcc 
      acagccact tgtcgcctg cacagacgt aaacgcgac 
      cgttaggac acgtgagca ggcttcagg accaagaaa 
      tgtgcattt tggtcggcc ttgaaccgc aaggaacga 
      caaacggac acattttat gcatctttg aggtgtttc 
      cgggtgaaa tgtcaacgc taggcgaaa tttatggcc 
      ttggaagga acgtttcgc cattgttta ctaatatta 
      gcacgtttc cagttgttt ctcttgttt gtgaaatgg 
      taaacaaca ataaatcaa ttttaggta aatctacca 
      ttttcacac ctgtaagtg gccaattaa gagtgtagg 
      aaaggtcag agattgtct tttgttttg ttttcaaaa 
      gtaataaac gtttaccaa gaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY