| General information | |||
| TC007475 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3936047..3942857 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007475 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04416 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007475 RA
MYGFVNYAL ELLVVKTFG EETWETIKK NADLQMEGS FLVRQIYED ELTYNLISA AVDVLQIPA NAILELFGK TFFEFCQDS GFDKILQVL GATPRDFLQ NLDALHDHL GTLYPGMKA PSFRCTVRP EDGALILHY YSDRPGLEH IVIGIVKTV ASKLHGTEV VVDILKTKE ECDHVQFLI TEASGPGKV QHPEMDEIQ TLSLEPKVN AATFCRVFP FHIMFDRDL KIVQTGSTV ARVIPKVTS AECRVTDIL DTVRPHLEL TFENILSHI NTIYVLKTR PGVMQVCAP PEYRYLRLK GQMLYVPET DLVIFLCYP SVMNLDDLT RRGLYISDI PLHDATRDL VLMSEQFEA DYKLTRNLE LLTDKLQQT YRELDREKK KTDGLLYSV LPISVANEL RHKRPVPAK RYDCVTLLF SGIVNFSAL CAANTDSKG VMKIVNMLN ELYTNFDVL TDPKKNPNI YKVETVGDK YMAVSGLPE PCTTHACNI ARLALDMMD RGKSVMFDG KPVKITIGI HSGEVVTGV IGHRMPRYC LFGNTVNLT SRTETTGEP GRINVSEEA YKFLCKEDN FDEQFHFEY RGPVTMKGK SEPMNVWFL SRAKSH
- >TC007475 RB
MYGFVNYAL ELLVVKTFG EETWETIKK NADLQMEGS FLVRQIYED ELTYNLISA AVDVLQIPA NAILELFGK TFFEFCQDS GFDKILQVL GATPRDFLQ NLDALHDHL GTLYPGMKA PSFRCTVRP EDGALILHY YSDRPGLEH IVIGIVKTV ASKLHGTEV VVDILKTKE ECDHVQFLI TEASGPGKV QHPEMDEIQ TLSLEPKVN AATFCRVFP FHIMFDRDL KIVQTGSTV ARVIPKVTS AECRVTDIL DTVRPHLEL TFENILSHI NTIYVLKTR PGVMQVCAP PEYRYLRLK GQMLYVPET DLVIFLCYP SVMNLDDLT RRGLYISDI PLHDATRDL VLMSEQFEA DYKLTRNLE LLTDKLQQT YRELDREKK KTDGLLYSV LPISVANEL RHKRPVPAK RYDCVTLLF SGIVNFSAL CAANTDSKG VMKIVNMLN ELYTNFDVL TDPKKNPNI YKVETVGDK YMAVSGLPE PCTTHACNI ARLALDMMD RGKSVMFDG KPVKITIGI HSGEVVTGV IGHRMPRYC LFGNTVNLT SRTETTGEP GRINVSEEA YKESGTERV RIPDGSWQF SQSWFRIVQ RVKCFSSGT WTPQRVKGI KPSSTKSG
- >TC007475 RA
atgtacggg tttgtgaac tatgctcta gaacttctc gtcgttaag acttttggc gaggaaact tgggagacg atcaaaaaa aatgccgac ctccaaatg gaaggctcg ttcctcgtc cggcaaatc tacgaggac gagctcaca tataacttg atttcagca gcggttgat gttttgcaa ataccagca aatgcaatt ttggaactc ttcggcaaa acttttttc gagttttgc caagattcc ggctttgat aagatcctt caagtgttg ggcgccact cctagggac tttctccag aatctggat gctctccac gaccattta ggcacccta tatccgggg atgaaagct ccgtctttc cggtgcacc gttcggcct gaagatggc gctttaatc ctgcactac tactcagac aggcccgga ctcgagcat attgtaatc gggatagtc aagaccgtt gcaagtaaa ttacacggg actgaagtt gtagttgac atcctcaaa accaaggaa gaatgcgat cacgtgcaa tttttgata accgaagct tcgggtccg ggcaaagtc cagcatccg gaaatggac gagatccaa acattatca ctcgagccc aaggtgaac gccgcgaca ttttgccgc gtgtttcct ttccacatc atgttcgac cgagacctg aagatcgtc caaacaggt tcaacggtg gcccgtgtc atcccaaag gtgacctcc gccgagtgc cgagtcacc gacatcctg gacacggtg cgtccccac ctggagctg accttcgag aacatcctc tcgcacata aacaccatc tacgtcctc aagacccgg cccggggtg atgcaagtc tgcgcgccc cccgagtat cgctacctc cgtctcaaa ggccaaatg ctctacgtg ccggagacc gacttggtg atcttcctc tgctacccc agcgtgatg aatctggac gaccttacg aggaggggg ctctacatc agcgacatt cctctacat gacgccaca cgcgatctg gtactcatg tccgagcaa ttcgaggct gattacaag ttgacgagg aacttggaa ctgctcacg gataagttg cagcagacg tacagggag ctggacagg gagaagaag aagacggac gggttgttg tactcggtg ctgcccatc agtgtggca aatgagctg agacacaag agaccagtg ccggccaag aggtacgac tgcgtcacg ctgcttttt tcaggaatt gttaacttc tccgccctg tgtgcggcc aataccgac tccaaagga gtcatgaag atcgtcaac atgctgaac gagctctac acgaatttt gatgtcttg acggatccc aaaaagaat ccaaatatt tacaaggtc gagactgtt ggcgacaaa tacatggcg gtgagcggc ctcccggag ccctgcacc actcacgct tgcaacatc gcccgttta gccctcgac atgatggac cgcggcaag tcggtcatg ttcgatggc aagcctgtg aaaatcaca attgggata cattctggt gaggttgtg acaggggtc ataggccac cgcatgccc cgatattgc ctttttggc aacacggtc aacttgaca agtcgcacg gaaacaacc ggcgaacct ggaaggatc aacgtttcg gaagaggcg tacaagttc ctgtgcaag gaggacaat ttcgatgag cagttccat ttcgagtat cgcgggccg gtgaccatg aagggcaag tctgaaccc atgaacgtg tggttcttg tcgcgagcc aagtcccac tag
- >TC007475 RB
atgtacggg tttgtgaac tatgctcta gaacttctc gtcgttaag acttttggc gaggaaact tgggagacg atcaaaaaa aatgccgac ctccaaatg gaaggctcg ttcctcgtc cggcaaatc tacgaggac gagctcaca tataacttg atttcagca gcggttgat gttttgcaa ataccagca aatgcaatt ttggaactc ttcggcaaa acttttttc gagttttgc caagattcc ggctttgat aagatcctt caagtgttg ggcgccact cctagggac tttctccag aatctggat gctctccac gaccattta ggcacccta tatccgggg atgaaagct ccgtctttc cggtgcacc gttcggcct gaagatggc gctttaatc ctgcactac tactcagac aggcccgga ctcgagcat attgtaatc gggatagtc aagaccgtt gcaagtaaa ttacacggg actgaagtt gtagttgac atcctcaaa accaaggaa gaatgcgat cacgtgcaa tttttgata accgaagct tcgggtccg ggcaaagtc cagcatccg gaaatggac gagatccaa acattatca ctcgagccc aaggtgaac gccgcgaca ttttgccgc gtgtttcct ttccacatc atgttcgac cgagacctg aagatcgtc caaacaggt tcaacggtg gcccgtgtc atcccaaag gtgacctcc gccgagtgc cgagtcacc gacatcctg gacacggtg cgtccccac ctggagctg accttcgag aacatcctc tcgcacata aacaccatc tacgtcctc aagacccgg cccggggtg atgcaagtc tgcgcgccc cccgagtat cgctacctc cgtctcaaa ggccaaatg ctctacgtg ccggagacc gacttggtg atcttcctc tgctacccc agcgtgatg aatctggac gaccttacg aggaggggg ctctacatc agcgacatt cctctacat gacgccaca cgcgatctg gtactcatg tccgagcaa ttcgaggct gattacaag ttgacgagg aacttggaa ctgctcacg gataagttg cagcagacg tacagggag ctggacagg gagaagaag aagacggac gggttgttg tactcggtg ctgcccatc agtgtggca aatgagctg agacacaag agaccagtg ccggccaag aggtacgac tgcgtcacg ctgcttttt tcaggaatt gttaacttc tccgccctg tgtgcggcc aataccgac tccaaagga gtcatgaag atcgtcaac atgctgaac gagctctac acgaatttt gatgtcttg acggatccc aaaaagaat ccaaatatt tacaaggtc gagactgtt ggcgacaaa tacatggcg gtgagcggc ctcccggag ccctgcacc actcacgct tgcaacatc gcccgttta gccctcgac atgatggac cgcggcaag tcggtcatg ttcgatggc aagcctgtg aaaatcaca attgggata cattctggt gaggttgtg acaggggtc ataggccac cgcatgccc cgatattgc ctttttggc aacacggtc aacttgaca agtcgcacg gaaacaacc ggcgaacct ggaaggatc aacgtttcg gaagaggcg tacaaggag tcgggcact gagagagtc cggattccg gacgggagc tggcagttc agccagagt tggttccgg atagtgcag cgggtcaag tgtttcagt tccgggact tggacgcca cagcgcgtc aagggaatt aaaccgtct tccaccaaa tccggataa
- >TC007475 RA
tggctgttt tcagctttt tcagtgctt tcagcgccg gcaaacacg caacagtgc caacaagaa cgacttgct ggtgcgcga ccatcatgt acgggtttg tgaactatg ctctagaac ttctcgtcg ttaagactt ttggcgagg aaacttggg agacgatca aaaaaaatg ccgacctcc aaatggaag gctcgttcc tcgtccggc aaatctacg aggacgagc tcacatata acttgattt cagcagcgg ttgatgttt tgcaaatac cagcaaatg caattttgg aactcttcg gcaaaactt ttttcgagt tttgccaag attccggct ttgataaga tccttcaag tgttgggcg ccactccta gggactttc tccagaatc tggatgctc tccacgacc atttaggca ccctatatc cggggatga aagctccgt ctttccggt gcaccgttc ggcctgaag atggcgctt taatcctgc actactact cagacaggc ccggactcg agcatattg taatcggga tagtcaaga ccgttgcaa gtaaattac acgggactg aagttgtag ttgacatcc tcaaaacca aggaagaat gcgatcacg tgcaatttt tgataaccg aagcttcgg gtccgggca aagtccagc atccggaaa tggacgaga tccaaacat tatcactcg agcccaagg tgaacgccg cgacatttt gccgcgtgt ttcctttcc acatcatgt tcgaccgag acctgaaga tcgtccaaa caggttcaa cggtggccc gtgtcatcc caaaggtga cctccgccg agtgccgag tcaccgaca tcctggaca cggtgcgtc cccacctgg agctgacct tcgagaaca tcctctcgc acataaaca ccatctacg tcctcaaga cccggcccg gggtgatgc aagtctgcg cgccccccg agtatcgct acctccgtc tcaaaggcc aaatgctct acgtgccgg agaccgact tggtgatct tcctctgct accccagcg tgatgaatc tggacgacc ttacgagga gggggctct acatcagcg acattcctc tacatgacg ccacacgcg atctggtac tcatgtccg agcaattcg aggctgatt acaagttga cgaggaact tggaactgc tcacggata agttgcagc agacgtaca gggagctgg acagggaga agaagaaga cggacgggt tgttgtact cggtgctgc ccatcagtg tggcaaatg agctgagac acaagagac cagtgccgg ccaagaggt acgactgcg tcacgctgc ttttttcag gaattgtta acttctccg ccctgtgtg cggccaata ccgactcca aaggagtca tgaagatcg tcaacatgc tgaacgagc tctacacga attttgatg tcttgacgg atcccaaaa agaatccaa atatttaca aggtcgaga ctgttggcg acaaataca tggcggtga gcggcctcc cggagccct gcaccactc acgcttgca acatcgccc gtttagccc tcgacatga tggaccgcg gcaagtcgg tcatgttcg atggcaagc ctgtgaaaa tcacaattg ggatacatt ctggtgagg ttgtgacag gggtcatag gccaccgca tgccccgat attgccttt ttggcaaca cggtcaact tgacaagtc gcacggaaa caaccggcg aacctggaa ggatcaacg tttcggaag aggcgtaca agttcctgt gcaaggagg acaatttcg atgagcagt tccatttcg agtatcgcg ggccggtga ccatgaagg gcaagtctg aacccatga acgtgtggt tcttgtcgc gagccaagt cccactaga gctaacaat ccaactagg tcagtatgg taattatgt tcggcacat gctttcttc attttcgtg agagacgat gtggtttca tcttttgta attgctcgt gtgaaatta cacgttccc tagagacaa gtggagcaa ttgttgatc aaaaatcgg gtaattgta ggacatggt aattacgag agcgcttgc gtaagcgaa tgaaagttg tgcgtcgat gggccctaa acttttcct gcaacctaa cagcatatt tttgttcca ggctgagcg gcgtcgtgc acactcttt aagtaccat tccatgtca gcgcgtgac aatctattc aagcgtatt gtttcagtt atgacatcg cattctcgt gtgtaatta tccccgaca agctacact tataaatat ctccgatag ataattttt aatcggttg tgtcagcac gcgtaatag aaaatccat gtcgactgt catcgctgt ctcttcagt gtgctttcc tttccacca ccgacataa cggccgaat tttactcga gccgcgccc acactttgc ccaaaattc gttcacctg tagagccac cttgaaggc aaggtgaac cgcctcatt ccacagcca cttgtcgcc tgcacagac gtaaacgcg accgttagg acacgtgag caggcttca ggaccaaga aatgtgcat tttggtcgg ccttgaacc gcaaggaac gacaaacgg acacatttt atgcatctt tgaggtgtt tccgggtga aatgtcaac gctaggcga aatttatgg ccttggaag gaacgtttc gccattgtt tactaatat tagcacgtt tccagttgt ttctcttgt ttgtgaaat ggtaaacaa caataaatc aattttagg taaatctac cattttcac acctgtaag tggccaatt aagagtgta ggaaaggtc agagattgt cttttgttt tgttttcaa aagtaataa acgtttacc aagaaa
- >TC007475 RB
tggctgttt tcagctttt tcagtgctt tcagcgccg gcaaacacg caacagtgc caacaagaa cgacttgct ggtgcgcga ccatcatgt acgggtttg tgaactatg ctctagaac ttctcgtcg ttaagactt ttggcgagg aaacttggg agacgatca aaaaaaatg ccgacctcc aaatggaag gctcgttcc tcgtccggc aaatctacg aggacgagc tcacatata acttgattt cagcagcgg ttgatgttt tgcaaatac cagcaaatg caattttgg aactcttcg gcaaaactt ttttcgagt tttgccaag attccggct ttgataaga tccttcaag tgttgggcg ccactccta gggactttc tccagaatc tggatgctc tccacgacc atttaggca ccctatatc cggggatga aagctccgt ctttccggt gcaccgttc ggcctgaag atggcgctt taatcctgc actactact cagacaggc ccggactcg agcatattg taatcggga tagtcaaga ccgttgcaa gtaaattac acgggactg aagttgtag ttgacatcc tcaaaacca aggaagaat gcgatcacg tgcaatttt tgataaccg aagcttcgg gtccgggca aagtccagc atccggaaa tggacgaga tccaaacat tatcactcg agcccaagg tgaacgccg cgacatttt gccgcgtgt ttcctttcc acatcatgt tcgaccgag acctgaaga tcgtccaaa caggttcaa cggtggccc gtgtcatcc caaaggtga cctccgccg agtgccgag tcaccgaca tcctggaca cggtgcgtc cccacctgg agctgacct tcgagaaca tcctctcgc acataaaca ccatctacg tcctcaaga cccggcccg gggtgatgc aagtctgcg cgccccccg agtatcgct acctccgtc tcaaaggcc aaatgctct acgtgccgg agaccgact tggtgatct tcctctgct accccagcg tgatgaatc tggacgacc ttacgagga gggggctct acatcagcg acattcctc tacatgacg ccacacgcg atctggtac tcatgtccg agcaattcg aggctgatt acaagttga cgaggaact tggaactgc tcacggata agttgcagc agacgtaca gggagctgg acagggaga agaagaaga cggacgggt tgttgtact cggtgctgc ccatcagtg tggcaaatg agctgagac acaagagac cagtgccgg ccaagaggt acgactgcg tcacgctgc ttttttcag gaattgtta acttctccg ccctgtgtg cggccaata ccgactcca aaggagtca tgaagatcg tcaacatgc tgaacgagc tctacacga attttgatg tcttgacgg atcccaaaa agaatccaa atatttaca aggtcgaga ctgttggcg acaaataca tggcggtga gcggcctcc cggagccct gcaccactc acgcttgca acatcgccc gtttagccc tcgacatga tggaccgcg gcaagtcgg tcatgttcg atggcaagc ctgtgaaaa tcacaattg ggatacatt ctggtgagg ttgtgacag gggtcatag gccaccgca tgccccgat attgccttt ttggcaaca cggtcaact tgacaagtc gcacggaaa caaccggcg aacctggaa ggatcaacg tttcggaag aggcgtaca aggagtcgg gcactgaga gagtccgga ttccggacg ggagctggc agttcagcc agagttggt tccggatag tgcagcggg tcaagtgtt tcagttccg ggacttgga cgccacagc gcgtcaagg gaattaaac cgtcttcca ccaaatccg gataatcct caaaaattt ttttggttt ctaaattga tttttggta ccactctga gaagaagtt ggaggcagg ctacaatat tgtaagggt agacgtggc aaaactcca ttagttttt tcatgataa agacgacca aacaatcct cggtcttcg ccccatatc ttaggaaaa tatggagct tgtcaggga gccccaatt gaagggcta ttttagccg gggtcgggt tatcactcc cgcaacttt ccaatggga cgtctataa acctcgtag agtctaatt ccagtcctc ctgtggtcg catggtcga taaaataca caacaaaaa ttagatttt cttcctgac tatcgaaat cgctcttcc agcccgcaa tataataag gggccctgt taccaaaaa tggccccaa tgaaaatcc tttcgtagg ttttctcac aagcgtaga tgctctcca acagggcta aaaccgtcc ctgtatctt tcaactgcc agtcgtagc agactttaa cagcgacgt acaaaatgt tatggtacc gacccatca tacagacgg aaccattgg ccgcccggt tcccttcat ggtacctca cgatggcca taatgcttt cacggacgg ccgattcgg gttcggtgc cggtaccta aagctagtt ttttgcccg tggtttata caaaaaatc ctcacctct ggcatagcc ttcaataag ggtactgat ataagtgac catcatttg gcgctttcg cctgtcatg gaggctcgt tctgggcga gttcgtaaa ctggtcgag gtagcattc gagaaggta gttcatgac actgacagc cctcgacca aaatgctag gaaacattt tattggatt aacttgcgg ggtttcggc ttaagtgca ctttgcagg ttcctgtgc aaggaggac aatttcgat gagcagttc catttcgag tatcgcggg ccggtgacc atgaagggc aagtctgaa cccatgaac gtgtggttc ttgtcgcga gccaagtcc cactagagc taacaatcc aactaggtc agtatggta attatgttc ggcacatgc tttcttcat tttcgtgag agacgatgt ggtttcatc ttttgtaat tgctcgtgt gaaattaca cgttcccta gagacaagt ggagcaatt gttgatcaa aaatcgggt aattgtagg acatggtaa ttacgagag cgcttgcgt aagcgaatg aaagttgtg cgtcgatgg gccctaaac ttttcctgc aacctaaca gcatatttt tgttccagg ctgagcggc gtcgtgcac actctttaa gtaccattc catgtcagc gcgtgacaa tctattcaa gcgtattgt ttcagttat gacatcgca ttctcgtgt gtaattatc cccgacaag ctacactta taaatatct ccgatagat aatttttaa tcggttgtg tcagcacgc gtaatagaa aatccatgt cgactgtca tcgctgtct cttcagtgt gctttcctt tccaccacc gacataacg gccgaattt tactcgagc cgcgcccac actttgccc aaaattcgt tcacctgta gagccacct tgaaggcaa ggtgaaccg cctcattcc acagccact tgtcgcctg cacagacgt aaacgcgac cgttaggac acgtgagca ggcttcagg accaagaaa tgtgcattt tggtcggcc ttgaaccgc aaggaacga caaacggac acattttat gcatctttg aggtgtttc cgggtgaaa tgtcaacgc taggcgaaa tttatggcc ttggaagga acgtttcgc cattgttta ctaatatta gcacgtttc cagttgttt ctcttgttt gtgaaatgg taaacaaca ataaatcaa ttttaggta aatctacca ttttcacac ctgtaagtg gccaattaa gagtgtagg aaaggtcag agattgtct tttgttttg ttttcaaaa gtaataaac gtttaccaa gaaa
