| General information | |||
| TC008009 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 3850585..3854330 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008009 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04414 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC008009 RA
MSKKEWFMV LCIFIFYLM MGAVFFQWA ESEEEKQRS ATKRIQRRI VWEHIEQIY NSKAPSLPD QKKFTEILS DYCGKPVMK EMTEIREDS NWDFYHSLF FVITVVSTI GYGNLAPTT TLTRIVMIF YGLIGIPLN GIVMVTLGN YFGRSFTKL YQRWKNSKT DEDDSTRLG LISQVILYL VPGFTFFIF LPAGFMVLF EGWSYDVAV YYAFVTLTT IGFGDYVAG IDQPPAISD FYYWMYKIF LLIWVIGGL GYVVMILGF ITQFFQSKK VKQIEQIIS ENIKKTPLR IRQELRSLL QEFLILKVR RGKGLDPFP PRRISRTAS CPDLDIYRN LNSPTMVRK RALSECPGY YNLQMFESH SDLGPDEQE QQNDLLFKV VDALSNIES DPEEGAYEN LSDSEILAS ERYGSKWSL KSQTLALPK SHRRRAISD IRVPSMHKV EARQDIWND RQRTFSEPS SETENRSEN LLTRFKNLI MPQKENKTD VEQGPRRAS MFTSAEDRY LRQTNRGRV SVLSTQQQD AFLEQTSIA DFIRALSAI TVPESILPP PSGPKRKLG TASLSPPKG PSPPRVRRL AIRPNPQAR RTSLMPETH SLQPENRRF SLRPVEENL LAPAPDTHS LQNPNRRFS LRPVGENLL APPPYTPKP PEEARNTRR FSRPFNVPT ITVSGISPV QRQVNKRDN KSD
- >TC008009 RB
MMSKKEWFM VLCIFIFYL MMGAVFFQW AESEEEKQR SATKRIQRR IVWEHIEQI YNSKAPSLP DQKKFTEIL SDYCGKPVM KEMTEIRED SNWDFYHSL FFVITVVST IGYGNLAPT TTLTRIVMI FYGLIGIPL NGIVMVTLG NYFGRSFTK LYQRWKNSK TDEDDSTRL GLISQVILY LVPGFTFFI FLPAGFMVL FEGWSYDVA VYYAFVTLT TIGFGDYVA GIDQPPAIS DFYYWMYKI FLLIWVIGG LGYVVMILG FITQFFQSK KVKQIEQII SENIKKTPL RIRQELRSL LQEFLILKV RRGKGLDPF PPRRISRTA SCPDLDIYR NLNSPTMVR KRALSECPG YYNLQMFES HSDLGPDEQ EQQNDLLFK VVDALSNIE SDPEEGAYE NLSDSEILA SERYGSKWS LKSQTLALP KSHRRRAIS DIRVPSMHK VEARQDIWN DRQRTFSEP SSETENRSE NLLTRFKNL IMPQKENKT DVEQGPRRA SMFTSAEDR YLRQTNRGR VSVLSTQQQ DAFLEQTSI ADFIRALSA ITVPESILP PPSGPKRKL GTASLSPPK GPSPPRVRR LAIRPNPQA RRTSLMPET HSLQPENRR FSLRPVEEN LLAPAPDTH SLQNPNRRF SLRPVGENL LAPPPYTPK PPEEARNTR RFSRPFNVP TITVSGISP VQRQVNKRD NKSD
- >TC008009 RA
atgtccaag aaggaatgg ttcatggtt ttgtgcatt tttattttt tatttgatg atgggggcg gtgttcttc caatgggcg gaatccgaa gaagagaaa caacgctca gccaccaaa cgaatccaa aggagaata gtctgggag cacatcgag cagatttac aacagcaaa gcaccttcg ctccctgac caaaaaaaa ttcaccgaa attttgagc gactactgc ggcaagccg gtcatgaaa gagatgact gaaatccgg gaagacagc aattgggat ttttaccac tcgttgttt ttcgttatc acagtggtg agtaccatc ggctatggt aatttggcc ccaaccacc actctgaca cgcatcgtc atgattttt tatggtctg atcggaata cccttaaat ggtattgtg atggtgact ctaggcaat tatttcgga agatcattt acgaaactg taccaacgc tggaaaaac agtaagaca gacgaggat gattccaca cggttggga ctcataagt caagtcatt ttgtacttg gtgccagga ttcacgttc ttcattttc ctgcccgct gggtttatg gtacttttc gagggctgg agctacgac gtggccgtt tattacgcc tttgtcacc ctcaccacc atcggcttt ggcgattat gttgcagga atcgaccaa cccccagcc atctccgat ttctactac tggatgtac aaaatattc ctcctaatt tgggtcatc ggaggcctg ggttacgtc gtcatgatc ctgggtttc ataacccag tttttccaa agcaaaaaa gtgaaacaa atcgagcaa atcatcagc gaaaacatc aaaaagacc ccgctccgc atccgacaa gaactccga tccttgctt caagaattc ctgatcttg aaagtgcga agagggaaa ggtctggac cccttcccc cctcgccga atcagccga actgcaagt tgtcccgat ttggacatt taccgcaac ttgaattcg ccgacaatg gtgcgaaaa cgcgccctt tccgaatgt ccaggttac tacaacctg caaatgttc gaatctcac tcggacttg ggccctgac gagcaagag cagcaaaac gatctcctc ttcaaagtc gtggacgcc ctcagcaat atagagtcc gaccctgaa gaaggcgct tatgagaat ttgtcagat tcggagatc ctcgcaagt gagagatac ggaagcaaa tggtccctc aaaagccaa actctcgca cttccgaaa tcgcaccgg agacgggcc attagtgac attcgggtg ccttccatg cacaaagtt gaggcccgg caagacatt tggaacgac cgacagcgg acgttctcg gaaccgtcc tcagagact gaaaaccgc tccgaaaac ctcctcact cgtttcaaa aacctcatc atgccccag aaagagaac aaaaccgac gtggagcaa ggccctcgc agagctagc atgttcacg agcgctgag gatcgctat ttaagacag acaaacagg gggagggtt tcggtcctc tcgacccaa cagcaagac gccttcctt gaacaaacc tcaatcgcc gatttcatt cgcgccctt tcggctatc acagtcccc gaatcaatc ctgccaccc ccaagcggc cctaagaga aagctgggg acggccagt ttatcgccc ccgaagggg ccctcgccc cctagggtc cggcgactg gcaatcaga ccgaacccc caagcccgg aggacgtcc ctcatgcct gagacgcac agtctgcag cccgaaaat agaagattt tcgttaagg cccgttgag gagaattta ctagctcct gcgcccgac actcacagt ctacagaat ccaaatagg agattctcg ttaaggccc gttggggag aatttacta gcacctccg ccgtacacg ccgaagcct ccggaggag gcccggaac acccggagg ttctcaagg ccgttcaat gttcccact atcaccgtg agtggcatt agccccgtg caacgacaa gtcaataaa agagataat aaaagtgat tga
- >TC008009 RB
atgatgtcc aagaaggaa tggttcatg gttttgtgc atttttatt ttttatttg atgatgggg gcggtgttc ttccaatgg gcggaatcc gaagaagag aaacaacgc tcagccacc aaacgaatc caaaggaga atagtctgg gagcacatc gagcagatt tacaacagc aaagcacct tcgctccct gaccaaaaa aaattcacc gaaattttg agcgactac tgcggcaag ccggtcatg aaagagatg actgaaatc cgggaagac agcaattgg gatttttac cactcgttg tttttcgtt atcacagtg gtgagtacc atcggctat ggtaatttg gccccaacc accactctg acacgcatc gtcatgatt ttttatggt ctgatcgga ataccctta aatggtatt gtgatggtg actctaggc aattatttc ggaagatca tttacgaaa ctgtaccaa cgctggaaa aacagtaag acagacgag gatgattcc acacggttg ggactcata agtcaagtc attttgtac ttggtgcca ggattcacg ttcttcatt ttcctgccc gctgggttt atggtactt ttcgagggc tggagctac gacgtggcc gtttattac gcctttgtc accctcacc accatcggc tttggcgat tatgttgca ggaatcgac caaccccca gccatctcc gatttctac tactggatg tacaaaata ttcctccta atttgggtc atcggaggc ctgggttac gtcgtcatg atcctgggt ttcataacc cagtttttc caaagcaaa aaagtgaaa caaatcgag caaatcatc agcgaaaac atcaaaaag accccgctc cgcatccga caagaactc cgatccttg cttcaagaa ttcctgatc ttgaaagtg cgaagaggg aaaggtctg gaccccttc ccccctcgc cgaatcagc cgaactgca agttgtccc gatttggac atttaccgc aacttgaat tcgccgaca atggtgcga aaacgcgcc ctttccgaa tgtccaggt tactacaac ctgcaaatg ttcgaatct cactcggac ttgggccct gacgagcaa gagcagcaa aacgatctc ctcttcaaa gtcgtggac gccctcagc aatatagag tccgaccct gaagaaggc gcttatgag aatttgtca gattcggag atcctcgca agtgagaga tacggaagc aaatggtcc ctcaaaagc caaactctc gcacttccg aaatcgcac cggagacgg gccattagt gacattcgg gtgccttcc atgcacaaa gttgaggcc cggcaagac atttggaac gaccgacag cggacgttc tcggaaccg tcctcagag actgaaaac cgctccgaa aacctcctc actcgtttc aaaaacctc atcatgccc cagaaagag aacaaaacc gacgtggag caaggccct cgcagagct agcatgttc acgagcgct gaggatcgc tatttaaga cagacaaac agggggagg gtttcggtc ctctcgacc caacagcaa gacgccttc cttgaacaa acctcaatc gccgatttc attcgcgcc ctttcggct atcacagtc cccgaatca atcctgcca cccccaagc ggccctaag agaaagctg gggacggcc agtttatcg cccccgaag gggccctcg ccccctagg gtccggcga ctggcaatc agaccgaac ccccaagcc cggaggacg tccctcatg cctgagacg cacagtctg cagcccgaa aatagaaga ttttcgtta aggcccgtt gaggagaat ttactagct cctgcgccc gacactcac agtctacag aatccaaat aggagattc tcgttaagg cccgttggg gagaattta ctagcacct ccgccgtac acgccgaag cctccggag gaggcccgg aacacccgg aggttctca aggccgttc aatgttccc actatcacc gtgagtggc attagcccc gtgcaacga caagtcaat aaaagagat aataaaagt gattga
- >TC008009 RA
acaaggacg tgaataaat tcgaaaact tcacacttg aatcaagaa atcagttcg tcggtacca tcagtcgag caatcgtca aaaaagcat gaaggataa tagagtcaa ccgcgcctc gaaactcgc cctttattg tcatcagag ttcgatcga tctgtgaca attcggaag tccctcatt acaagaagt ggataatca cactcataa acgctcaat tgagaataa taccattta cgtgatttg catcgaaga tgatgtcca agaaggaat ggttcatgg ttttgtgca tttttattt tttatttga tgatggggg cggtgttct tccaatggg cggaatccg aagaagaga aacaacgct cagccacca aacgaatcc aaaggagaa tagtctggg agcacatcg agcagattt acaacagca aagcacctt cgctccctg accaaaaaa aattcaccg aaattttga gcgactact gcggcaagc cggtcatga aagagatga ctgaaatcc gggaagaca gcaattggg atttttacc actcgttgt ttttcgtta tcacagtgg tgagtacca tcggctatg gtaatttgg ccccaacca ccactctga cacgcatcg tcatgattt tttatggtc tgatcggaa tacccttaa atggtattg tgatggtga ctctaggca attatttcg gaagatcat ttacgaaac tgtaccaac gctggaaaa acagtaaga cagacgagg atgattcca cacggttgg gactcataa gtcaagtca ttttgtact tggtgccag gattcacgt tcttcattt tcctgcccg ctgggttta tggtacttt tcgagggct ggagctacg acgtggccg tttattacg cctttgtca ccctcacca ccatcggct ttggcgatt atgttgcag gaatcgacc aacccccag ccatctccg atttctact actggatgt acaaaatat tcctcctaa tttgggtca tcggaggcc tgggttacg tcgtcatga tcctgggtt tcataaccc agtttttcc aaagcaaaa aagtgaaac aaatcgagc aaatcatca gcgaaaaca tcaaaaaga ccccgctcc gcatccgac aagaactcc gatccttgc ttcaagaat tcctgatct tgaaagtgc gaagaggga aaggtctgg accccttcc cccctcgcc gaatcagcc gaactgcaa gttgtcccg atttggaca tttaccgca acttgaatt cgccgacaa tggtgcgaa aacgcgccc tttccgaat gtccaggtt actacaacc tgcaaatgt tcgaatctc actcggact tgggccctg acgagcaag agcagcaaa acgatctcc tcttcaaag tcgtggacg ccctcagca atatagagt ccgaccctg aagaaggcg cttatgaga atttgtcag attcggaga tcctcgcaa gtgagagat acggaagca aatggtccc tcaaaagcc aaactctcg cacttccga aatcgcacc ggagacggg ccattagtg acattcggg tgccttcca tgcacaaag ttgaggccc ggcaagaca tttggaacg accgacagc ggacgttct cggaaccgt cctcagaga ctgaaaacc gctccgaaa acctcctca ctcgtttca aaaacctca tcatgcccc agaaagaga acaaaaccg acgtggagc aaggccctc gcagagcta gcatgttca cgagcgctg aggatcgct atttaagac agacaaaca gggggaggg tttcggtcc tctcgaccc aacagcaag acgccttcc ttgaacaaa cctcaatcg ccgatttca ttcgcgccc tttcggcta tcacagtcc ccgaatcaa tcctgccac ccccaagcg gccctaaga gaaagctgg ggacggcca gtttatcgc ccccgaagg ggccctcgc cccctaggg tccggcgac tggcaatca gaccgaacc cccaagccc ggaggacgt ccctcatgc ctgagacgc acagtctgc agcccgaaa atagaagat tttcgttaa ggcccgttg aggagaatt tactagctc ctgcgcccg acactcaca gtctacaga atccaaata ggagattct cgttaaggc ccgttgggg agaatttac tagcacctc cgccgtaca cgccgaagc ctccggagg aggcccgga acacccgga ggttctcaa ggccgttca atgttccca ctatcaccg tgagtggca ttagccccg tgcaacgac aagtcaata aaagagata ataaaagtg attgagagt tggcctgta tcaggtcga gtgctcaat tctttctgt gattacttg tgtctcaag tgcctgtaa ctatttatg aataaagaa atatattat tt
- >TC008009 RB
acaaggacg tgaataaat tcgaaaact tcacacttg aatcaagaa atcagttcg tcggtacca tcagtcgag caatcgtca aaaaagcat gaaggataa tagagtcaa ccgcgcctc gaaactcgc cctttattg tcatcagag ttcgatcga tctgtgaca attcggaag tccctcatt acaagaagt ggataatca cactcataa acgctcaat tgagaataa taccattta cgtgatttg catcgaaga tgatgtcca agaaggaat ggttcatgg ttttgtgca tttttattt tttatttga tgatggggg cggtgttct tccaatggg cggaatccg aagaagaga aacaacgct cagccacca aacgaatcc aaaggagaa tagtctggg agcacatcg agcagattt acaacagca aagcacctt cgctccctg accaaaaaa aattcaccg aaattttga gcgactact gcggcaagc cggtcatga aagagatga ctgaaatcc gggaagaca gcaattggg atttttacc actcgttgt ttttcgtta tcacagtgg tgagtacca tcggctatg gtaatttgg ccccaacca ccactctga cacgcatcg tcatgattt tttatggtc tgatcggaa tacccttaa atggtattg tgatggtga ctctaggca attatttcg gaagatcat ttacgaaac tgtaccaac gctggaaaa acagtaaga cagacgagg atgattcca cacggttgg gactcataa gtcaagtca ttttgtact tggtgccag gattcacgt tcttcattt tcctgcccg ctgggttta tggtacttt tcgagggct ggagctacg acgtggccg tttattacg cctttgtca ccctcacca ccatcggct ttggcgatt atgttgcag gaatcgacc aacccccag ccatctccg atttctact actggatgt acaaaatat tcctcctaa tttgggtca tcggaggcc tgggttacg tcgtcatga tcctgggtt tcataaccc agtttttcc aaagcaaaa aagtgaaac aaatcgagc aaatcatca gcgaaaaca tcaaaaaga ccccgctcc gcatccgac aagaactcc gatccttgc ttcaagaat tcctgatct tgaaagtgc gaagaggga aaggtctgg accccttcc cccctcgcc gaatcagcc gaactgcaa gttgtcccg atttggaca tttaccgca acttgaatt cgccgacaa tggtgcgaa aacgcgccc tttccgaat gtccaggtt actacaacc tgcaaatgt tcgaatctc actcggact tgggccctg acgagcaag agcagcaaa acgatctcc tcttcaaag tcgtggacg ccctcagca atatagagt ccgaccctg aagaaggcg cttatgaga atttgtcag attcggaga tcctcgcaa gtgagagat acggaagca aatggtccc tcaaaagcc aaactctcg cacttccga aatcgcacc ggagacggg ccattagtg acattcggg tgccttcca tgcacaaag ttgaggccc ggcaagaca tttggaacg accgacagc ggacgttct cggaaccgt cctcagaga ctgaaaacc gctccgaaa acctcctca ctcgtttca aaaacctca tcatgcccc agaaagaga acaaaaccg acgtggagc aaggccctc gcagagcta gcatgttca cgagcgctg aggatcgct atttaagac agacaaaca gggggaggg tttcggtcc tctcgaccc aacagcaag acgccttcc ttgaacaaa cctcaatcg ccgatttca ttcgcgccc tttcggcta tcacagtcc ccgaatcaa tcctgccac ccccaagcg gccctaaga gaaagctgg ggacggcca gtttatcgc ccccgaagg ggccctcgc cccctaggg tccggcgac tggcaatca gaccgaacc cccaagccc ggaggacgt ccctcatgc ctgagacgc acagtctgc agcccgaaa atagaagat tttcgttaa ggcccgttg aggagaatt tactagctc ctgcgcccg acactcaca gtctacaga atccaaata ggagattct cgttaaggc ccgttgggg agaatttac tagcacctc cgccgtaca cgccgaagc ctccggagg aggcccgga acacccgga ggttctcaa ggccgttca atgttccca ctatcaccg tgagtggca ttagccccg tgcaacgac aagtcaata aaagagata ataaaagtg attgagagt tggcctgta tcaggtcga gtgctcaat tctttctgt gattacttg tgtctcaag tgcctgtaa ctatttatg aataaagaa atatattat tt
