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Gene information
General information
TC007999 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3702485..3704071
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007999
    iBeetle screen iB_04411

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007999 RA
      MEVMDVVVP EKDEGSPAE IKPNLQDCD MEDLYTKYK 
      KLQQMLEFL EVQEEYIKD EQRNLKKEY LHAQEEVKR 
      IQSVPLVIG QFLEAVDQN TGIVGSTTG SNYYVRILS 
      TIDRELLKP SASVALHKH SNALVDVLP PEADSSISM 
      LQADEKPDV QYSDIGGMD MQKQEIREA VELPLTHFE 
      LYKQIGIDP PRGVLMYGP PGCGKTMLA KAVAHHTTA 
      AFIRVVGSE FVQKYLGEG PRMVRDVFR LAKENSPAI 
      IFIDEIDAI ATKRFDAQT GADREVQRI LLELLNQMD 
      GFDQTTNVK VIMATNRAD TLDPALLRP GRLDRKIEF 
      PLPDRRQKR LIFSTITSK MNLSEEVDL EDFVARPDR 
      ISGADINAI CQEAGMHAV RENRYIVLP KDFEKGYKN 
      NIKKDESEH EFYK
    CDS sequence
    • >TC007999 RA
      atggaagtg atggatgtt gtagtgccc gaaaaggat 
      gagggaagc cctgctgaa atcaagccc aacctccag 
      gactgcgac atggaagac ctgtacacc aaatacaag 
      aaactgcaa caaatgctc gaatttctc gaagtccaa 
      gaagagtac atcaaagac gagcaacgc aacttgaag 
      aaggagtat ctccacgcc caggaggag gtgaagcgg 
      atccagtcg gtgccgctt gtcatcggc cagtttctg 
      gaagccgtt gaccaaaat actgggatt gtggggtca 
      accaccggg tcgaattat tacgtccgg attttgtca 
      acgattgac cgggaattg ctcaaacct tcggccagt 
      gtggctttg cataagcac agtaacgcc cttgtggac 
      gtcctcccg cctgaagct gattcctcg atcagcatg 
      ctccaggct gatgagaag cctgacgtc cagtatagc 
      gatattgga ggcatggat atgcaaaaa caggaaatt 
      cgcgaagct gtggagttg cccctcacc cacttcgag 
      ctgtacaag caaattggg attgacccc cctcgtggg 
      gtcttaatg tacgggcct ccaggctgt ggcaagaca 
      atgctggcc aaggcagtg gctcaccac accactgct 
      gcgttcatc agagttgtg gggtcggag ttcgtccag 
      aagtaccta ggggagggg cctaggatg gttcgggac 
      gtgttccga ctggctaag gagaattca ccagctatt 
      atcttcatt gacgagatt gatgccatt gctactaag 
      cggtttgat gcccagacg ggggccgac agagaagta 
      caaaggatt ctcttggag ttgctcaac caaatggat 
      ggttttgat caaaccacc aatgttaaa gttattatg 
      gctacaaat agggctgat accctcgac ccggccctg 
      cttcggccg ggccgtttg gacaggaaa atcgagttt 
      ccattgccc gacaggagg caaaaaaga ttgatcttt 
      agcaccatt acgtcaaaa atgaactta tcggaagag 
      gtggacttg gaggatttc gttgcccga cctgaccgt 
      atttcaggt gctgatatt aatgccatt tgtcaagag 
      gcaggaatg catgccgtg agggaaaat cggtatatt 
      gtgctgccg aaagatttc gaaaaaggc tacaaaaat 
      aatattaag aaggatgaa agcgagcac gaattttat 
      aaataa
    mRNA sequence
    • >TC007999 RA
      cctctctaa aacaaccaa aaatcttcc aaatcatcg 
      taacataac cccaattct ttgtaattc acgtatttt 
      tgcaaacaa tggaagtga tggatgttg tagtgcccg 
      aaaaggatg agggaagcc ctgctgaaa tcaagccca 
      acctccagg actgcgaca tggaagacc tgtacacca 
      aatacaaga aactgcaac aaatgctcg aatttctcg 
      aagtccaag aagagtaca tcaaagacg agcaacgca 
      acttgaaga aggagtatc tccacgccc aggaggagg 
      tgaagcgga tccagtcgg tgccgcttg tcatcggcc 
      agtttctgg aagccgttg accaaaata ctgggattg 
      tggggtcaa ccaccgggt cgaattatt acgtccgga 
      ttttgtcaa cgattgacc gggaattgc tcaaacctt 
      cggccagtg tggctttgc ataagcaca gtaacgccc 
      ttgtggacg tcctcccgc ctgaagctg attcctcga 
      tcagcatgc tccaggctg atgagaagc ctgacgtcc 
      agtatagcg atattggag gcatggata tgcaaaaac 
      aggaaattc gcgaagctg tggagttgc ccctcaccc 
      acttcgagc tgtacaagc aaattggga ttgaccccc 
      ctcgtgggg tcttaatgt acgggcctc caggctgtg 
      gcaagacaa tgctggcca aggcagtgg ctcaccaca 
      ccactgctg cgttcatca gagttgtgg ggtcggagt 
      tcgtccaga agtacctag gggaggggc ctaggatgg 
      ttcgggacg tgttccgac tggctaagg agaattcac 
      cagctatta tcttcattg acgagattg atgccattg 
      ctactaagc ggtttgatg cccagacgg gggccgaca 
      gagaagtac aaaggattc tcttggagt tgctcaacc 
      aaatggatg gttttgatc aaaccacca atgttaaag 
      ttattatgg ctacaaata gggctgata ccctcgacc 
      cggccctgc ttcggccgg gccgtttgg acaggaaaa 
      tcgagtttc cattgcccg acaggaggc aaaaaagat 
      tgatcttta gcaccatta cgtcaaaaa tgaacttat 
      cggaagagg tggacttgg aggatttcg ttgcccgac 
      ctgaccgta tttcaggtg ctgatatta atgccattt 
      gtcaagagg caggaatgc atgccgtga gggaaaatc 
      ggtatattg tgctgccga aagatttcg aaaaaggct 
      acaaaaata atattaaga aggatgaaa gcgagcacg 
      aattttata aataaattg tttaaggtc tttttgctt 
      atttaattt tgtatgtgt aactaatgt ctgaattaa 
      aatagtaat aaccga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY