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Gene information
General information
TC007966 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3240670..3247395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007966
    iBeetle screen iB_04408

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007966 RA
      MLLETRWNL IFGCVLFCG FFEKIHCAT GQPNVVFIV 
      ADDLGWNDV GFHGSNQIP TPNIDALAY NGIILNSHY 
      SQSFGTPSR AALLTGKYP MKLGLQGPS ITPAEGRSL 
      PEGKIMSEY FKDMGYATH LVGKWHLGH SRWNDTPTF 
      RGFDHFFGF YNGFTSYYD YVSNWKIND KEYSGFDLR 
      RDTVPSWND AGKYATDLF AEHAVDVIQ KHNVNTPLF 
      MMIAHLAVH VGNEGKWLE APQETVNKF KHIRDPNRR 
      TYAAMVSKL DDSIGAVFE ALEAKNMLQ NTIVVFISD 
      NGAPTVGPH HNWGSNYPL RGIKDTLFE GGVRTVACI 
      WSPLLVQSS RVSTDLIHI TDWLPTLFT AVGGDLSVL 
      DPDLDGIDQ WSSLVYDLP SARNDIPLN IDEKTRNAA 
      LRFSYWKLI VGTSGNGSY NGYFGAPLN ENIEEQQYN 
      TSAINDSPV GRIAKKINY NPLSETDFD GLRRVATLK 
      CLDAKAKRN PCDPASGAV CLYNIPNDP CEENDLAKF 
      FPSVVRRIK RTLVDYRQG LVPQIETSI DIENADPKL 
      FQFAWNPWR DCSDAACTS
    CDS sequence
    • >TC007966 RA
      atgttgctg gaaacgcga tggaacttg atttttgga 
      tgtgtgttg ttttgcggt tttttcgaa aaaattcac 
      tgtgccacc ggacaacca aacgttgtt ttcatcgtt 
      gctgatgac ttgggttgg aatgatgtg ggtttccac 
      ggaagtaac cagattcca acacccaac attgacgcg 
      ctcgcatac aatggcata attctcaat agccattac 
      agtcaatca ttcggtact ccttcaaga gccgcttta 
      ttaacagga aaatatcca atgaaatta ggactgcaa 
      ggaccttcg ataacgcca gctgaagga cgatcctta 
      cctgaagga aaaataatg tccgagtat ttcaaggac 
      atgggctat gccacccat ctcgtgggc aaatggcat 
      ctgggccac tcccgttgg aatgacact ccaacgttc 
      cgaggattc gaccatttc ttcgggttc tataacgga 
      ttcaccagt tactacgac tacgtctcc aactggaag 
      atcaatgat aaggaatat agcggtttt gacttgcga 
      agagacacg gtgcccagt tggaacgat gctggaaaa 
      tacgcaacg gatttattt gccgaacac gccgttgac 
      gtgatccaa aaacacaat gtcaatacg ccgcttttt 
      atgatgatt gctcatcta gccgtccat gtaggaaat 
      gaggggaaa tggctggag gctccccaa gaaaccgtc 
      aataaattc aaacatatc cgggacccc aatcggagg 
      acctacgca gcaatggtc tcgaaactt gacgacagc 
      atcggtgcc gtgtttgaa gccttggag gcgaaaaac 
      atgttacag aacacaatt gtcgttttt atttccgac 
      aatggcgcg ccgacagtg ggcccccac cacaactgg 
      gggtctaat tatccttta cgaggcatt aaagacacc 
      ctgtttgag ggcggagtg cgtacggtg gcatgcata 
      tggagtcct ttgctggtt caatcgtca cgcgtttcc 
      accgacctc atccacata accgactgg ctaccgact 
      ctctttacc gcagtcggt ggcgacttg agtgtcttg 
      gacccagac cttgacggc atagaccaa tggtccagt 
      ctagtttac gacctacca tcggcccgc aacgacatt 
      cctctaaac atcgacgaa aaaaccaga aacgcggct 
      ttgcgattc tcttactgg aaactgata gttggcacc 
      agtggtaac gggagttac aatggttac ttcggagcc 
      ccgctcaat gaaaatatc gaggagcaa caatacaac 
      acttcggcg attaatgac agccccgtt ggccgcatt 
      gcgaaaaaa atcaattac aatccgctt tcagaaacc 
      gactttgat ggtttacgg agagttgcg accctgaaa 
      tgtctggac gcgaaagca aaacgaaat ccatgtgat 
      cctgcaagt ggagctgtc tgtttgtat aacataccg 
      aatgatccg tgtgaggag aatgatcta gccaagttt 
      tttccgagc gttgtgcgg agaattaaa cggactctg 
      gtggattat aggcaaggg ttagttcca caaattgag 
      acgagcatt gatatcgaa aatgctgat cccaaactg 
      ttccaattc gcctggaat ccgtggcgc gactgctcg 
      gacgctgcc tgcactagc tga
    mRNA sequence
    • >TC007966 RA
      atgttgctg gaaacgcga tggaacttg atttttgga 
      tgtgtgttg ttttgcggt tttttcgaa aaaattcac 
      tgtgccacc ggacaacca aacgttgtt ttcatcgtt 
      gctgatgac ttgggttgg aatgatgtg ggtttccac 
      ggaagtaac cagattcca acacccaac attgacgcg 
      ctcgcatac aatggcata attctcaat agccattac 
      agtcaatca ttcggtact ccttcaaga gccgcttta 
      ttaacagga aaatatcca atgaaatta ggactgcaa 
      ggaccttcg ataacgcca gctgaagga cgatcctta 
      cctgaagga aaaataatg tccgagtat ttcaaggac 
      atgggctat gccacccat ctcgtgggc aaatggcat 
      ctgggccac tcccgttgg aatgacact ccaacgttc 
      cgaggattc gaccatttc ttcgggttc tataacgga 
      ttcaccagt tactacgac tacgtctcc aactggaag 
      atcaatgat aaggaatat agcggtttt gacttgcga 
      agagacacg gtgcccagt tggaacgat gctggaaaa 
      tacgcaacg gatttattt gccgaacac gccgttgac 
      gtgatccaa aaacacaat gtcaatacg ccgcttttt 
      atgatgatt gctcatcta gccgtccat gtaggaaat 
      gaggggaaa tggctggag gctccccaa gaaaccgtc 
      aataaattc aaacatatc cgggacccc aatcggagg 
      acctacgca gcaatggtc tcgaaactt gacgacagc 
      atcggtgcc gtgtttgaa gccttggag gcgaaaaac 
      atgttacag aacacaatt gtcgttttt atttccgac 
      aatggcgcg ccgacagtg ggcccccac cacaactgg 
      gggtctaat tatccttta cgaggcatt aaagacacc 
      ctgtttgag ggcggagtg cgtacggtg gcatgcata 
      tggagtcct ttgctggtt caatcgtca cgcgtttcc 
      accgacctc atccacata accgactgg ctaccgact 
      ctctttacc gcagtcggt ggcgacttg agtgtcttg 
      gacccagac cttgacggc atagaccaa tggtccagt 
      ctagtttac gacctacca tcggcccgc aacgacatt 
      cctctaaac atcgacgaa aaaaccaga aacgcggct 
      ttgcgattc tcttactgg aaactgata gttggcacc 
      agtggtaac gggagttac aatggttac ttcggagcc 
      ccgctcaat gaaaatatc gaggagcaa caatacaac 
      acttcggcg attaatgac agccccgtt ggccgcatt 
      gcgaaaaaa atcaattac aatccgctt tcagaaacc 
      gactttgat ggtttacgg agagttgcg accctgaaa 
      tgtctggac gcgaaagca aaacgaaat ccatgtgat 
      cctgcaagt ggagctgtc tgtttgtat aacataccg 
      aatgatccg tgtgaggag aatgatcta gccaagttt 
      tttccgagc gttgtgcgg agaattaaa cggactctg 
      gtggattat aggcaaggg ttagttcca caaattgag 
      acgagcatt gatatcgaa aatgctgat cccaaactg 
      ttccaattc gcctggaat ccgtggcgc gactgctcg 
      gacgctgcc tgcactagc tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY