Status
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Gene information
General information
TC007953 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3166288..3169514
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC007953
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
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      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04407

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
      Days post injection



      adult lethality - Number of animals showing the phenotype: 9 (22 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (22 dpi)
      Days post injection


      Adult morphology
      elytra colour irregular - Number of animals showing the phenotype: 5


      elytra surface irregular - Number of animals showing the phenotype: 5


      elytra not closed - Number of animals showing the phenotype: 5

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC007953 RA
        MVEFALNTT DSVLKIRLF VRSSHNCLF QNHTGMVQS 
        PHQQSEQNA VFRATYHPP PQEYYNGQA NPSTGAVPR 
        QSWHGGDNK STPRPYPED FSQSNSQQV TVQIEQPKE 
        QKDKKSMTK TYHTIKDII SSRFKSSKD NTEEKSEEP 
        GLNNVAEEL RRSQRNIGE EQQEKKTEQ NIYGKPRID 
        PNISHQQHQ YNQHIMLQA QQYQVNQQL KMQQTMYQQ 
        QLVQARSQE MLVARPEEQ VYYQNAYGA TPQRPGNRF 
        VSPANREQN YVQMHHSQF PAKEKDDRR QPIAMERRS 
        AQQIERDSQ RQKSFEARR AASHPQLAY DDETKTEVP 
        DSRPQPQAA PMRRGSHGN IMEAAVSTA PDAEKDSDD 
        GGFLKRNNS KEKKNPEIN QEEDSKVTE ALQGTPRKR 
        LEGEIGKIE GVYNVGQRS KDDEGRGKK NPSGSAASS 
        DYDKAGQSS SNADSGRGS AAYSSGRRP GGLDLNGEG 
        DTGQLQGHY RDHHGGHDS EWVDIVENE LRHILEPKL 
        HELSLQGGV GIANSTLSE SISSMTPPL PPLSPGEQS 
        SPNVTPRNS TRYKPSSLP YGSKPDYDG YKSKLHTGR 
        ETSVNSRWH NNSNQKHRS TKKSDHSTA LRGKQIFGL 
        DTTDLTSTT TRSLDLESM LDGQSDSDG DISTTDARA 
        IRKQLEGLE TMYSEVLKL LGVKKYSGR YQPSDPRFS 
        KRRYGSMSS LPSSSVSSR PIRDKRRAH EDRKKVRDI 
        RGINKRFQR LESHVVTLA RSVAHLSSE MRTQHLMIQ 
        EMENIRGEI AALRTQTNM LNVRSQSAS RAVNTSKDL 
        PTLANPTRV KKLTKFFGD EPPLLRLFL RKLGYEKYA 
        NVFESERVG MVELPYLSE ERLQKMGVP LGPRLRIMQ 
        EAQISVCKD NTLCIV
      CDS sequence
      • >TC007953 RA
        atggtcgaa tttgcttta aacaccaca gattcggta 
        ttaaaaatt cgcttgttt gtgcgctcc tcacataat 
        tgcttgttt cagaatcac acgggaatg gtacaatcc 
        ccacatcag caatccgag caaaacgca gtcttccga 
        gccacttac cacccacct ccgcaagaa tattacaac 
        ggacaagcc aatccatca actggtgct gtgccgaga 
        cagtcgtgg catgggggc gacaacaaa tccactcca 
        cgcccttac ccagaagac ttctcccaa agcaactcc 
        caacaagtg accgtacaa atcgaacaa ccgaaagaa 
        caaaaagac aaaaagagt atgacaaag acataccac 
        accatcaaa gacatcatt tcgagtagg ttcaagagt 
        agcaaagac aacaccgag gaaaaaagt gaagaaccc 
        ggcttgaac aacgtggcg gaggaatta aggcgtagc 
        caaagaaac ataggagag gaacaacaa gaaaaaaag 
        acagaacaa aacatctac ggcaaaccc agaattgat 
        ccgaacatt tcgcaccaa cagcaccaa tacaatcag 
        cacatcatg ctccaagcg cagcaatac caggtcaac 
        caacagctc aaaatgcaa caaacgatg taccagcag 
        cagctagtg caagcaagg agccaggaa atgttagtc 
        gcaagaccg gaagaacaa gtctattac cagaatgcc 
        tacggggcg acgccccaa aggcccgga aatagattc 
        gtctcgccg gcgaatcgg gaacagaac tacgtacaa 
        atgcaccat tcgcaattt ccagccaaa gaaaaggac 
        gaccgacgc caaccaatc gctatggag cgccgcagc 
        gcccaacaa atcgagcga gacagccag cgtcagaaa 
        agtttcgaa gctcgacgt gccgcctcc cacccccag 
        ttggcctac gacgacgaa accaaaacc gaagtcccc 
        gacagccgt ccccagcct caagcagcc cccatgcgg 
        cgtggttcc cacgggaac atcatggaa gcggctgtg 
        tcaaccgcc cccgatgcg gaaaaagac agcgacgat 
        ggcggtttt ctcaaacga aacaattcc aaagagaag 
        aaaaatccc gaaatcaat caagaggaa gacagtaaa 
        gtgacagaa gcccttcag ggaactccg cgtaagcgt 
        ctggaaggc gaaatcggg aagatcgaa ggcgtgtat 
        aatgttggc cagcgatcg aaggacgat gaagggaga 
        gggaagaag aatccttca ggatcggct gcgagttcc 
        gattatgat aaagcggga caatcgtcg tcgaatgcc 
        gactctggg agaggcagt gctgcgtat agtagtggg 
        aggaggcct ggagggctg gacttgaat ggggaaggg 
        gacacgggg cagttgcaa ggacactac agggatcat 
        cacggaggg catgattcc gaatgggtg gatatagtc 
        gagaatgaa ttgaggcat attcttgag ccaaaattg 
        cacgaattg tcgttacaa gggggcgtc ggaattgcg 
        aattcgacg cttagtgag agcatttcg tcgatgact 
        ccgccactt ccgccactt tcgcccggc gaacagtct 
        tcgcccaat gtcactccg aggaattcg acgagatat 
        aaacccagc agtttgcca tatggaagt aaaccggat 
        tatgatggc tacaagtcc aagcttcac acaggcagg 
        gaaacgagc gtgaattcc cgatggcac aataactcc 
        aaccagaaa catcgcagt acaaaaaag agtgaccat 
        agtaccgcc ttgaggggc aaacaaatt tttggcttg 
        gataccacc gatttgact tcaaccacg acccggtcg 
        ttggatctc gagtcaatg ttggacggt caaagcgac 
        tcagacggc gacataagc acaaccgat gccagagcc 
        atcagaaag cagttggag ggtttggag actatgtat 
        tcggaagtc ctcaaactc cttggcgtt aaaaaatac 
        agtggtcgt taccagcct tcagatcct aggtttagt 
        aagcggcgc tacggcagt atgtcttcg ctgccgtcc 
        agctcggtc agcagtcgg ccgatccga gacaagcgg 
        cgcgcccac gaagaccgg aagaaagtc agagacatt 
        cggggaatt aacaagcgg tttcaaagg ctggaatcg 
        cacgttgtg actctagcg aggtcggtc gctcattta 
        tcttccgag atgcggacc cagcattta atgattcaa 
        gaaatggag aacattcgg ggtgaaata gcggcgctg 
        aggacccag accaacatg ctgaatgtg aggtcgcag 
        tcggcgtca cgggccgtc aacacgtcc aaagactta 
        ccgactttg gcgaacccc acaagagtc aaaaagttg 
        acgaagttt tttggagat gagccgcca ttattgaga 
        ctgttttta cggaaactt ggctatgag aaatacgca 
        aacgttttc gagagtgag cgagtcggg atggtcgaa 
        ctgccttat cttagtgaa gagcgactt cagaagatg 
        ggggtgccg ttgggccca agactcagg atcatgcag 
        gaagcgcag atttcggtc tgcaaggat aacacgttg 
        tgtatagtt tag
      mRNA sequence
      • >TC007953 RA
        gttcgttac aaaatggtc gaatttgct ttaaacacc 
        acagattcg gtattaaaa attcgcttg tttgtgcgc 
        tcctcacat aattgcttg tttcagaat cacacggga 
        atggtacaa tccccacat cagcaatcc gagcaaaac 
        gcagtcttc cgagccact taccaccca cctccgcaa 
        gaatattac aacggacaa gccaatcca tcaactggt 
        gctgtgccg agacagtcg tggcatggg ggcgacaac 
        aaatccact ccacgccct tacccagaa gacttctcc 
        caaagcaac tcccaacaa gtgaccgta caaatcgaa 
        caaccgaaa gaacaaaaa gacaaaaag agtatgaca 
        aagacatac cacaccatc aaagacatc atttcgagt 
        aggttcaag agtagcaaa gacaacacc gaggaaaaa 
        agtgaagaa cccggcttg aacaacgtg gcggaggaa 
        ttaaggcgt agccaaaga aacatagga gaggaacaa 
        caagaaaaa aagacagaa caaaacatc tacggcaaa 
        cccagaatt gatccgaac atttcgcac caacagcac 
        caatacaat cagcacatc atgctccaa gcgcagcaa 
        taccaggtc aaccaacag ctcaaaatg caacaaacg 
        atgtaccag cagcagcta gtgcaagca aggagccag 
        gaaatgtta gtcgcaaga ccggaagaa caagtctat 
        taccagaat gcctacggg gcgacgccc caaaggccc 
        ggaaataga ttcgtctcg ccggcgaat cgggaacag 
        aactacgta caaatgcac cattcgcaa tttccagcc 
        aaagaaaag gacgaccga cgccaacca atcgctatg 
        gagcgccgc agcgcccaa caaatcgag cgagacagc 
        cagcgtcag aaaagtttc gaagctcga cgtgccgcc 
        tcccacccc cagttggcc tacgacgac gaaaccaaa 
        accgaagtc cccgacagc cgtccccag cctcaagca 
        gcccccatg cggcgtggt tcccacggg aacatcatg 
        gaagcggct gtgtcaacc gcccccgat gcggaaaaa 
        gacagcgac gatggcggt tttctcaaa cgaaacaat 
        tccaaagag aagaaaaat cccgaaatc aatcaagag 
        gaagacagt aaagtgaca gaagccctt cagggaact 
        ccgcgtaag cgtctggaa ggcgaaatc gggaagatc 
        gaaggcgtg tataatgtt ggccagcga tcgaaggac 
        gatgaaggg agagggaag aagaatcct tcaggatcg 
        gctgcgagt tccgattat gataaagcg ggacaatcg 
        tcgtcgaat gccgactct gggagaggc agtgctgcg 
        tatagtagt gggaggagg cctggaggg ctggacttg 
        aatggggaa ggggacacg gggcagttg caaggacac 
        tacagggat catcacgga gggcatgat tccgaatgg 
        gtggatata gtcgagaat gaattgagg catattctt 
        gagccaaaa ttgcacgaa ttgtcgtta caagggggc 
        gtcggaatt gcgaattcg acgcttagt gagagcatt 
        tcgtcgatg actccgcca cttccgcca ctttcgccc 
        ggcgaacag tcttcgccc aatgtcact ccgaggaat 
        tcgacgaga tataaaccc agcagtttg ccatatgga 
        agtaaaccg gattatgat ggctacaag tccaagctt 
        cacacaggc agggaaacg agcgtgaat tcccgatgg 
        cacaataac tccaaccag aaacatcgc agtacaaaa 
        aagagtgac catagtacc gccttgagg ggcaaacaa 
        atttttggc ttggatacc accgatttg acttcaacc 
        acgacccgg tcgttggat ctcgagtca atgttggac 
        ggtcaaagc gactcagac ggcgacata agcacaacc 
        gatgccaga gccatcaga aagcagttg gagggtttg 
        gagactatg tattcggaa gtcctcaaa ctccttggc 
        gttaaaaaa tacagtggt cgttaccag ccttcagat 
        cctaggttt agtaagcgg cgctacggc agtatgtct 
        tcgctgccg tccagctcg gtcagcagt cggccgatc 
        cgagacaag cggcgcgcc cacgaagac cggaagaaa 
        gtcagagac attcgggga attaacaag cggtttcaa 
        aggctggaa tcgcacgtt gtgactcta gcgaggtcg 
        gtcgctcat ttatcttcc gagatgcgg acccagcat 
        ttaatgatt caagaaatg gagaacatt cggggtgaa 
        atagcggcg ctgaggacc cagaccaac atgctgaat 
        gtgaggtcg cagtcggcg tcacgggcc gtcaacacg 
        tccaaagac ttaccgact ttggcgaac cccacaaga 
        gtcaaaaag ttgacgaag ttttttgga gatgagccg 
        ccattattg agactgttt ttacggaaa cttggctat 
        gagaaatac gcaaacgtt ttcgagagt gagcgagtc 
        gggatggtc gaactgcct tatcttagt gaagagcga 
        cttcagaag atgggggtg ccgttgggc ccaagactc 
        aggatcatg caggaagcg cagatttcg gtctgcaag 
        gataacacg ttgtgtata gtttaggga agggacagt 
        cagacaata cgtaggaaa tttaaccta gttttaagt 
        ttgctaaga attttttaa atatttatt tagttagta 
        attttagtt gtctgatct gtaagtgat tgtaaatgg 
        cggctcaaa aaagacctc ggttttttt agttataat 
        tttgtgtgt acttaaaga tgtatttat acgacgatt 
        tttgtacat attgaaata aacagttaa gttattgcc 
        aataatacc aaagatttg ttctttgta cttttctac 
        gtgaaataa aacataatg ggttttgta acaaacgct 
        tttatttat tttttcaat aaaagactc catataca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY