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Gene information
General information
TC007951 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3086177..3091800
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007951
    iBeetle screen iB_04406

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007951 RA
      MATGRTVRQ VKQGEKLRR SQNSLNPIS GPDSNIEHN 
      EGVAKVVKT SAFVEVEGY GPYKPVPPP KPLPQQPIS 
      SQQGCLTPP PYRMPPYPL YNEPSIPGA TGLDTSPAT 
      QGLHTHSSK FPIEREVIL SSTDKNSKI PILHDYKKR 
      TKGAIAPDV PPKQMAAWT AQGQTHQIL SPDQTYMTV 
      CDKVEDIFD RNVCSEAQA TICTDLLIK SEVPRKLVN 
      LNLKSLYDK LCRYDLKNL IIVGQFIAD ATSANCVAS 
      CLFGLENLS FYGNEIKIL RRFTFPAAE KLRKLSLVK 
      NRIENVDFS SFSLLNVAN IDLSYNNIQ AIEEGTFNH 
      TSLSVVNLQ HNQIAFLDP GSFGANLEL LDLSYNKLV 
      SIKDQVFAH TKNIRELIL SHNQLWIVP DLNQMSDLR 
      AVGLSNNEI RTVYFQEFR SVRDLRLLD LSHNKLDEA 
      TEVLKVFAN KNHLWALSL AFNNLENID FSAFNNVTT 
      GFLMLYGNP WRCNKWDQV ERILRLNFI NWAQCDFNL 
      FSSGFFPYC IADSIEDSE VESARKRFR DAVTHELLK 
      MCHIDIHSE RYNLLYPHG TEYMCF
    CDS sequence
    • >TC007951 RA
      atggcaacg ggtcgtacc gtacgtcaa gtcaagcaa 
      ggagagaag ttgaggagg tcacaaaac tcgcttaat 
      cccatttca ggacccgac agcaacatc gaacacaat 
      gaaggggtt gctaaagtg gtcaaaacg agcgctttt 
      gttgaggtg gaaggttat gggccctac aaaccggtt 
      ccaccccca aaacctcta ccccaacag cctatttcg 
      tcccaacaa ggctgtctc acaccgccc ccctacagg 
      atgcccccg taccccctg tacaatgag cctagtatt 
      ccgggagcc acaggttta gatactagt cctgccaca 
      caagggctg cacacacac tccagcaag tttccgatc 
      gaaagagaa gtgattttg tccagtacg gacaagaac 
      tcgaagatc cccattctt cacgactac aagaaacga 
      acgaaaggt gccatcgct cctgacgtc ccacccaaa 
      caaatggct gcgtggacg gcacagggc caaacccac 
      cagattctg agccctgat cagacctac atgacagtt 
      tgtgataaa gtggaggac attttcgac cgcaacgtg 
      tgttcggag gcacaagca acaatttgt accgatttg 
      ctgataaaa tctgaagtt ccccgaaaa cttgtaaac 
      ttgaatttg aaatccctt tacgacaag ctttgccgg 
      tacgacttg aaaaacttg ataatagtt ggtcagttt 
      atagcagac gccacatct gcgaattgc gtggctagt 
      tgtctgttt ggtttagaa aacttgagt ttttacggt 
      aacgaaatt aagatcctg cgtcggttt acgttccca 
      gcggcagaa aaattacgc aaattgtct ctggtaaaa 
      aataggatt gaaaatgtg gacttttcg agtttttca 
      ttgttgaat gttgcgaac atcgacttg tcctacaat 
      aacatccaa gctattgaa gaagggact tttaatcac 
      acctcgctt tcggtggtt aatttgcaa cacaaccag 
      attgcattt cttgatcct ggcagtttt ggcgcaaac 
      ttggaactg cttgatttg agttacaac aagttggtg 
      tcaataaaa gaccaagtg tttgcacac acaaaaaac 
      attcgagaa ttgattttg tcgcataat cagctctgg 
      atcgtcccg gatttaaac caaatgagc gatttacga 
      gcagtgggg ctctccaac aatgagatc agaacggtt 
      tacttccaa gagttccga agtgttcgc gatttgagg 
      ttgcttgac ttaagtcac aacaaatta gatgaggcc 
      actgaagtt ttgaaagtt tttgctaat aaaaaccat 
      ctatgggcg ctgtccttg gctttcaac aacttggaa 
      aatattgac tttagtgct ttcaataat gtgactaca 
      ggatttttg atgctgtac ggaaatccc tggaggtgt 
      aataaatgg gatcaagta gagaggatt ttgagactt 
      aattttatt aattgggcc cagtgtgac tttaacttg 
      ttcagttct gggtttttc ccatattgc attgccgat 
      tccattgaa gatagtgaa gtggaatct gcaagaaaa 
      cgttttcgt gatgcagtt acacatgaa ctgctgaaa 
      atgtgtcat atagacatt cattccgag cgctataac 
      ttgctctac cctcacggc actgaatat atgtgcttc 
      tag
    mRNA sequence
    • >TC007951 RA
      ccggtgttc cgccatctg aagacgttc gctgttcgc 
      aaaccgtaa ccataagac tgaaagagg tccgacttc 
      ccgcttttc tatgttaat aatctaaga tcagacagg 
      gcccccctc tttcacgcg accataaac cgtctaaaa 
      caatacggc aatggttta gttcagtgg cggtaccgc 
      cggtagaaa aatcgcgga aagttgtgc gagtgcgag 
      cggaagatg tcaccatgt aatggcaac gggtcgtac 
      cgtacgtca agtcaagca aggagagaa gttgaggag 
      gtcacaaaa ctcgcttaa tcccatttc aggacccga 
      cagcaacat cgaacacaa tgaaggggt tgctaaagt 
      ggtcaaaac gagcgcttt tgttgaggt ggaaggtta 
      tgggcccta caaaccggt tccaccccc aaaacctct 
      accccaaca gcctatttc gtcccaaca aggctgtct 
      cacaccgcc cccctacag gatgccccc gtaccccct 
      gtacaatga gcctagtat tccgggagc cacaggttt 
      agatactag tcctgccac acaagggct gcacacaca 
      ctccagcaa gtttccgat cgaaagaga agtgatttt 
      gtccagtac ggacaagaa ctcgaagat ccccattct 
      tcacgacta caagaaacg aacgaaagg tgccatcgc 
      tcctgacgt cccacccaa acaaatggc tgcgtggac 
      ggcacaggg ccaaaccca ccagattct gagccctga 
      tcagaccta catgacagt ttgtgataa agtggagga 
      cattttcga ccgcaacgt gtgttcgga ggcacaagc 
      aacaatttg taccgattt gctgataaa atctgaagt 
      tccccgaaa acttgtaaa cttgaattt gaaatccct 
      ttacgacaa gctttgccg gtacgactt gaaaaactt 
      gataatagt tggtcagtt tatagcaga cgccacatc 
      tgcgaattg cgtggctag ttgtctgtt tggtttaga 
      aaacttgag tttttacgg taacgaaat taagatcct 
      gcgtcggtt tacgttccc agcggcaga aaaattacg 
      caaattgtc tctggtaaa aaataggat tgaaaatgt 
      ggacttttc gagtttttc attgttgaa tgttgcgaa 
      catcgactt gtcctacaa taacatcca agctattga 
      agaagggac ttttaatca cacctcgct ttcggtggt 
      taatttgca acacaacca gattgcatt tcttgatcc 
      tggcagttt tggcgcaaa cttggaact gcttgattt 
      gagttacaa caagttggt gtcaataaa agaccaagt 
      gtttgcaca cacaaaaaa cattcgaga attgatttt 
      gtcgcataa tcagctctg gatcgtccc ggatttaaa 
      ccaaatgag cgatttacg agcagtggg gctctccaa 
      caatgagat cagaacggt ttacttcca agagttccg 
      aagtgttcg cgatttgag gttgcttga cttaagtca 
      caacaaatt agatgaggc cactgaagt tttgaaagt 
      ttttgctaa taaaaacca tctatgggc gctgtcctt 
      ggctttcaa caacttgga aaatattga ctttagtgc 
      tttcaataa tgtgactac aggattttt gatgctgta 
      cggaaatcc ctggaggtg taataaatg ggatcaagt 
      agagaggat tttgagact taattttat taattgggc 
      ccagtgtga ctttaactt gttcagttc tgggttttt 
      cccatattg cattgccga ttccattga agatagtga 
      agtggaatc tgcaagaaa acgttttcg tgatgcagt 
      tacacatga actgctgaa aatgtgtca tatagacat 
      tcattccga gcgctataa cttgctcta ccctcacgg 
      cactgaata tatgtgctt ctagagttg aagattgac 
      gttttctct ttacaataa agaagtttc aaacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY