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Gene information
General information
TC007945 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3046117..3047314
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007945
    iBeetle screen iB_04404

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007945 RA
      MKLQLLAGI FFATVCYTT ADQEKTCPE 
      NEEYKTCGT ACPPTCQNK SPQICTDNC 
      VIGCFCKKG YVREAPGGR CVPESCENV 
      PVCGKNEVF STCATACPK TCDDSEPKI 
      CPLVCLTGC VCKDNYVRE SKGGKCVPE 
      VACKKECGE NEIYNECGS LCPGTCSQP 
      VKVCEKKCV KGCFCKEGY ILDDKTRKC 
      VKRDECPKR S
    CDS sequence
    • >TC007945 RA
      atgaagctt caactgctt gctggtatt tttttcgcg 
      acggtgtgt tacaccact gccgaccaa gaaaaaact 
      tgtcccgaa aacgaagaa tacaaaact tgtggaacc 
      gcttgtccc cccacttgc caaaacaaa tcgccgcaa 
      atttgcacc gataattgc gttattggg tgtttttgc 
      aagaaagga tacgtccgg gaggcaccc ggaggtcgc 
      tgtgtgcca gaatcgtgc gaaaatgtt ccagtttgt 
      ggaaaaaat gaagtattc agcacgtgc gccaccgcg 
      tgtcccaaa acttgcgac gacagtgag cccaaaatt 
      tgtcctttg gtgtgtctg accggatgc gtttgtaag 
      gataattat gttagggag agtaaaggt gggaaatgt 
      gttccggag gttgcttgc aagaaagaa tgtggtgaa 
      aatgagatc tacaacgaa tgcgggtct ttgtgtcct 
      ggaacctgc tctcagccg gtgaaagtt tgtgagaag 
      aaatgtgtc aaagggtgc ttttgcaag gagggatac 
      attctagat gataaaacg cgaaaatgc gtcaagaga 
      gacgagtgt cccaagcgc tcctaa
    mRNA sequence
    • >TC007945 RA
      actaattag taaacacgt ctgctgagt tggaaacaa 
      ataacaaat tttccctgc ctggaaaac tacacacaa 
      taaaaaaca aacaaatcc ttaaacttg cttaagcag 
      ttaatataa aaataaatc tctgatgtg tagtcaaat 
      aatctcgtt gcaaatatt ttactcata actttattt 
      gtttgttat tgtgttcgt tgaacgcaa tgatgttat 
      aaatattcg cagacgaga atttgctta aagtgagaa 
      tcagagtta gacaaaatg aagcttcaa ctgcttgct 
      ggtattttt ttcgcgacg gtgtgttac accactgcc 
      gaccaagaa aaaacttgt cccgaaaac gaagaatac 
      aaaacttgt ggaaccgct tgtcccccc acttgccaa 
      aacaaatcg ccgcaaatt tgcaccgat aattgcgtt 
      attgggtgt ttttgcaag aaaggatac gtccgggag 
      gcacccgga ggtcgctgt gtgccagaa tcgtgcgaa 
      aatgttcca gtttgtgga aaaaatgaa gtattcagc 
      acgtgcgcc accgcgtgt cccaaaact tgcgacgac 
      agtgagccc aaaatttgt cctttggtg tgtctgacc 
      ggatgcgtt tgtaaggat aattatgtt agggagagt 
      aaaggtggg aaatgtgtt ccggaggtt gcttgcaag 
      aaagaatgt ggtgaaaat gagatctac aacgaatgc 
      gggtctttg tgtcctgga acctgctct cagccggtg 
      aaagtttgt gagaagaaa tgtgtcaaa gggtgcttt 
      tgcaaggag ggatacatt ctagatgat aaaacgcga 
      aaatgcgtc aagagagac gagtgtccc aagcgctcc 
      taatttttt tcataatta ataaagtga accatgtac 
      tgaaatttt gaacttgga ataaaaata cattgtttg 
      cccaccaaa cgtgtcaca agtgtatgg tagataaaa 
      atctacatg atagtctct ttaaatatt tacaaaaaa 
      acaagcggc tttaatttg caatgctat ttaaataaa 
      tgaatgcaa aaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY