Status
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Gene information
General information
TC007938 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2954924..2958822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007938
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04403

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007938 RA
      MMTDTLNSN HVMALSGKV DIDTKMDDM GWKAKLKIP 
      PKDRRIQTS DVTDTRGNE FEEFCLKRE LLMGIFEKG 
      WEKPSPIQE ASIPIALSG KDILARAKN GTGKTGAYS 
      IPVLEQIDP KKDCIQALI IVPTRELAL QTSQICIEL 
      AKHLDVRVM VTTGGTNLR DDIMRIYQK VQVIIATPG 
      RILDLMEKG VAVMDQCKI LVLDEADKL LSQDFKGML 
      DTVIKNLPQ ERQILLFSA TFPLTVEQF MRKHLRDPY 
      EINLMEELT LKGVTQYYA FVQERQKVH CLNTLFSKL 
      QINQSIIFC NSTQRVELL AKKITELGY CCYYIHAKM 
      AQAHRNRVF HDFRAGLCR NLVCSDLFT RGIDVQAVN 
      VVINFDFPK MAETYLHRI GRSGRFGHL GIAINLITY 
      DDRFALHRI EQELGTEIK PIPKVIDPK LYVAKLIDD 
      EDTPEDNAK
    CDS sequence
    • >TC007938 RA
      atgatgacg gacacattg aattcgaac catgtgatg 
      gctttgtca ggcaaagtt gatattgac accaaaatg 
      gatgacatg ggatggaaa gccaaactg aagattcca 
      cccaaggat aggagaata cagaccagt gatgtgact 
      gataccagg ggaaatgaa tttgaagaa ttctgcttg 
      aagagagaa ttattaatg ggtattttt gaaaaaggg 
      tgggagaaa ccgtctcct atccaagaa gcttctatt 
      ccaattgcg ttatctggc aaggatatt cttgcaagg 
      gcaaaaaat ggcacaggt aaaaccggt gcgtacagt 
      attcccgtg ttggaacag atcgatccc aagaaagac 
      tgcattcaa gcactgatc atagtgcct acacgagag 
      ctcgcgctt caaacgtca caaatttgc attgagctc 
      gctaagcac ttggacgtg cgcgtgatg gtaacgacc 
      ggtggaaca aatttaaga gatgacata atgcgcatc 
      taccagaaa gtacaagtt attatagca acgcctggc 
      cgcatcctc gatctgatg gaaaagggt gtcgccgta 
      atggaccag tgcaagatt ctagtttta gacgaagcc 
      gacaagctt ctctcacaa gacttcaag gggatgctc 
      gacactgtg ataaagaac ttaccgcaa gagagacaa 
      atactgttg ttctccgcc acgtttcct ctcacagtt 
      gagcagttt atgcgaaaa catctccga gatccgtac 
      gagatcaat ctcatggag gagctcact cttaaaggt 
      gtcacgcaa tactatgcg ttcgttcaa gaacgtcag 
      aaagtacac tgtttaaat actctgttt tcaaagtta 
      caaataaac caaagcata atattctgc aattccacg 
      caacgtgtc gaattgttg gcgaagaaa atcaccgaa 
      cttggttat tgctgctac tacattcac gccaagatg 
      gctcaagcg catcgcaac agagtattc catgatttt 
      cgagctggt ctctgccga aatctagtc tgttctgat 
      ctgtttaca agaggtatc gatgtgcaa gccgtcaac 
      gtcgtcatc aatttcgac ttcccgaaa atggcagag 
      acgtatctg caccgtatc ggtagatct ggaagattt 
      gggcacttg ggcattgcc atcaacttg attacttac 
      gatgatcga ttcgcgctt catcgcatt gaacaagag 
      ttgggaacg gagatcaag ccgatcccg aaagtgatc 
      gatcccaag ttgtacgtg gccaagtta atagacgac 
      gaagatact ccagaagac aacgccaag taa
    mRNA sequence
    • >TC007938 RA
      cggtattcc ttaaattct ttgtccaga atcagatta 
      ttatcgttc ctgtgaggg atcttagga actgtactt 
      atcctacac attgtagag gaagaagat ttgggtttt 
      ataaaccct attttttaa tacacttgc aatagtatt 
      ttctacaat tatgatgac ggacacatt gaattcgaa 
      ccatgtgat ggctttgtc aggcaaagt tgatattga 
      caccaaaat ggatgacat gggatggaa agccaaact 
      gaagattcc acccaagga taggagaat acagaccag 
      tgatgtgac tgataccag gggaaatga atttgaaga 
      attctgctt gaagagaga attattaat gggtatttt 
      tgaaaaagg gtgggagaa accgtctcc tatccaaga 
      agcttctat tccaattgc gttatctgg caaggatat 
      tcttgcaag ggcaaaaaa tggcacagg taaaaccgg 
      tgcgtacag tattcccgt gttggaaca gatcgatcc 
      caagaaaga ctgcattca agcactgat catagtgcc 
      tacacgaga gctcgcgct tcaaacgtc acaaatttg 
      cattgagct cgctaagca cttggacgt gcgcgtgat 
      ggtaacgac cggtggaac aaatttaag agatgacat 
      aatgcgcat ctaccagaa agtacaagt tattatagc 
      aacgcctgg ccgcatcct cgatctgat ggaaaaggg 
      tgtcgccgt aatggacca gtgcaagat tctagtttt 
      agacgaagc cgacaagct tctctcaca agacttcaa 
      ggggatgct cgacactgt gataaagaa cttaccgca 
      agagagaca aatactgtt gttctccgc cacgtttcc 
      tctcacagt tgagcagtt tatgcgaaa acatctccg 
      agatccgta cgagatcaa tctcatgga ggagctcac 
      tcttaaagg tgtcacgca atactatgc gttcgttca 
      agaacgtca gaaagtaca ctgtttaaa tactctgtt 
      ttcaaagtt acaaataaa ccaaagcat aatattctg 
      caattccac gcaacgtgt cgaattgtt ggcgaagaa 
      aatcaccga acttggtta ttgctgcta ctacattca 
      cgccaagat ggctcaagc gcatcgcaa cagagtatt 
      ccatgattt tcgagctgg tctctgccg aaatctagt 
      ctgttctga tctgtttac aagaggtat cgatgtgca 
      agccgtcaa cgtcgtcat caatttcga cttcccgaa 
      aatggcaga gacgtatct gcaccgtat cggtagatc 
      tggaagatt tgggcactt gggcattgc catcaactt 
      gattactta cgatgatcg attcgcgct tcatcgcat 
      tgaacaaga gttgggaac ggagatcaa gccgatccc 
      gaaagtgat cgatcccaa gttgtacgt ggccaagtt 
      aatagacga cgaagatac tccagaaga caacgccaa 
      gtaacgcgg tgtgttacg aaatatcac tgataactg 
      taaatatta aacaacgta ctttccgaa gaattaaat 
      ttctgttga agacttttt tatactaaa aaagggtga 
      tatactgat ttatttaag agattctgc actactacg 
      taacttatc acagtgatt ctacatttg gggcctaag 
      ctttaaatc gcataagtt gttacccgt gtgcattta 
      aagctgcaa aattctaca gtcactatt ttacaacgc 
      catgaaatt cgttactta ccatccgaa tctccgcga 
      atttgatgg ccgcgtaaa accgcactg tgatacgtt 
      cgagtcgtc ggtgcagat ctagttctt aaactataa 
      gttgaacag tgtgaactt tgatagtga aaaactaaa 
      atgtgttaa aactgagaa aagactcct aagtgtgct 
      aataatgtg acttgggtt cagggaacg agaagaaag 
      aagaggagt gatagtgct ccaacaatt tttccgagg 
      agattctta ttacgattc ggtggggcc tccgaattg 
      tgacgtgtt caagcattg cgaaaaagg cccaaaatt 
      ctgtttggg ggatgtcca aaattatcc tagaattgt 
      ttattaccg aatcttccg caaattgat tctttaaca 
      ccgaatttt aaagacttg tttttcatt tttactaag 
      ggggggtta tttatgacg tcgttgtgc taaatgttt 
      gcaaaaatt ttattgtct tgctttata tacggtctt 
      atttatttt tttactatt gggtaacag atttttgca 
      tacatttaa cattgatat tttgttaaa aaataataa 
      aaaacgtga ggggtgttt cactaaatt tgtgttagg 
      attattcta ttttaagtt atctaaagg tacagtatt 
      aatacttgc agtggtctt tataaaaat agtcgccct 
      attgtggtt gttaatcaa gtttgttac tttatatca 
      atattgttg ctgtaaatt tttttcaaa aaataaatg 
      cccttcaca cac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY