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Gene information
General information
TC007935 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2924474..2926218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007935
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04401

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007935 RA
      MSRSGYTCV RHLFCWLNV LLWITGCGI LGVGIWLRI 
      AYEGYASLL PQYALLSAD SLAIAVGTI TFILAFLAC 
      CGSWFQSRC MLVTYFSLV IFMFIAEFL VGSLAFAFR 
      ISLGHTFRQ ELQNGLWNH YNMTAKGPD SLVTIWDNL 
      QSEFKCCGV DNYQDWFMI NAWPNRTWV PDSCCLPWK 
      AGENCGKSG DANDFYMTG CYHQIHMWF VQRLHVVGI 
      VGLLVAFIQ LFGLISSML LFCTVRHKR TSRTYKSYQ 
      THT
    CDS sequence
    • >TC007935 RA
      atgtctaga agtggatat acatgtgtt aggcattta 
      ttttgttgg ctgaatgtt ctactttgg atcacaggt 
      tgtggaatt ttaggagtt ggaatctgg ttgcggata 
      gcttacgag ggctatgcc tctttgctg ccccaatac 
      gctctactt agtgccgac tcacttgcg atagcagtc 
      ggtacaata acgtttatt ttggctttt cttgcatgc 
      tgcggctct tggtttcaa agcagatgt atgctagtt 
      acctatttt agtctggtt atatttatg tttatagcc 
      gagtttctt gtcggatcg ctagctttc gcgtttcgc 
      atttcattg gggcacacg tttcggcaa gagctgcaa 
      aatgggctc tggaaccat tataatatg acggccaaa 
      gggcctgat agcttggtt acgatttgg gataactta 
      cagtcggaa tttaaatgt tgcggtgtg gataattat 
      caagactgg tttatgata aacgcttgg ccgaatagg 
      acttgggtt cctgattcg tgttgcctt ccttggaaa 
      gcaggcgaa aattgtggc aagtcagga gacgcaaat 
      gatttttat atgactggt tgttaccac caaatccat 
      atgtggttt gtgcagaga cttcatgtt gtgggcatt 
      gtggggctg ctggtagct ttcatacag ctttttgga 
      cttatatca tcgatgttg ctgttttgt acagtcaga 
      cacaaaagg acttcacgg acttataaa tcctaccaa 
      acacacaca tag
    mRNA sequence
    • >TC007935 RA
      gtatgaacc tgaccttgt agtgtttac ttgtgaccg 
      actttctaa taacgtgtt tgatgacgc aaaaagata 
      ctcacggat actctatgt tattaaaca atatcttat 
      ctattgatt ctcaaagaa tcataccta attcataaa 
      aattaatct caaaagtaa ataagggaa cactactgt 
      agatctgcc taaaggatc tgaaaaaaa tgtctagaa 
      gtggatata catgtgtta ggcatttat tttgttggc 
      tgaatgttc tactttgga tcacaggtt gtggaattt 
      taggagttg gaatctggt tgcggatag cttacgagg 
      gctatgcct ctttgctgc cccaatacg ctctactta 
      gtgccgact cacttgcga tagcagtcg gtacaataa 
      cgtttattt tggcttttc ttgcatgct gcggctctt 
      ggtttcaaa gcagatgta tgctagtta cctatttta 
      gtctggtta tatttatgt ttatagccg agtttcttg 
      tcggatcgc tagctttcg cgtttcgca tttcattgg 
      ggcacacgt ttcggcaag agctgcaaa atgggctct 
      ggaaccatt ataatatga cggccaaag ggcctgata 
      gcttggtta cgatttggg ataacttac agtcggaat 
      ttaaatgtt gcggtgtgg ataattatc aagactggt 
      ttatgataa acgcttggc cgaatagga cttgggttc 
      ctgattcgt gttgccttc cttggaaag caggcgaaa 
      attgtggca agtcaggag acgcaaatg atttttata 
      tgactggtt gttaccacc aaatccata tgtggtttg 
      tgcagagac ttcatgttg tgggcattg tggggctgc 
      tggtagctt tcatacagc tttttggac ttatatcat 
      cgatgttgc tgttttgta cagtcagac acaaaagga 
      cttcacgga cttataaat cctaccaaa cacacacat 
      agatttcaa agtatttaa tttatttaa tcatcatgt 
      tcgcaagat tacgtagac tgaaagttt taatcgatt 
      tgtaatctt gccattgtg ccaaagttc gtcacatca 
      aattaacaa tccgcagcg ttacattca cagttaaca 
      ttatatatc acagaaaag ttattgcaa ctgttaaaa 
      attatatct ccaactata acatttata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY