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Gene information
General information
TC007922 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2778944..2781823
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007922
    iBeetle screen iB_04399

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages sometimes randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax complete not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007922 RA
      MKIHQCTVS TFLPSILVL FFVNFGFTQ DAPIIKLPN 
      GLIKGRVGQ TIAKRPYWA YQKIPFATP PLGDLRFAA 
      PVPSKNWDG VLETTKYDV ICYQITSDS DLESEDCLY 
      LNVYTPTDP SNKTNRGLP VMFFIYGGG FIEGNCFDY 
      VYGPEYLLD RGVIVVCAN YRVGPFGFL STGDMTVPG 
      NNGLKDQLL ALQWTHDNI HLFGGDPTK VTIFGQSAG 
      SASVAYHLL HTQSQGLFR AVICESGSF LSPWAYQRN 
      ARNYAFTTA GLIDEVVGK SNDSQTLLD FLRSVSAKE 
      LDQASYKLS LTEAHKNRQ ILQGFFFAP VIEPKHDGA 
      FISQKMYVQ LKKGDFIRV PTLMGFNSE ENIFHTYLK 
      LWFESALNT YDKNIDWLV PNDMRINDS EKRKQVGIK 
      IRDIYTNGS DLLENGPSS VRYYSDTSY TRPIRKHAE 
      IQSQFSDVF FYQFSYDGK LGNWSFTVK GAENVSHGE 
      DNCYIWRKY DDYYDNSDL CRFPLPDRI TQYRIQTIW 
      TNFAKYLNP TPKKSKLLQ NITWPKFDS KAQYVDILK 
      NLEIKSEPK KETYDQWIE LYDSLGYDD FDTY
    CDS sequence
    • >TC007922 RA
      atgaaaatc catcagtgt accgtttcc accttctta 
      ccctcaatt ttagtgttg ttttttgtg aattttgga 
      ttcacacag gatgcgccc attatcaaa ttaccaaac 
      ggacttatt aaagggcgt gtaggtcaa accatagcc 
      aaacggcct tactgggca taccagaaa atacctttt 
      gcaacacca cctcttggt gatttacga tttgcagca 
      cctgttccc tcaaaaaat tgggatggg gttttggag 
      accacgaaa tatgatgtt atttgttac caaatcacc 
      agtgattcg gatttagag tcggaagat tgtctttat 
      ttaaatgtg tacactccg acagaccct tcaaataaa 
      acaaaccgg ggacttcca gttatgttc tttatttat 
      gggggaggt tttattgaa ggaaattgt tttgactat 
      gtgtacggt cctgaatat ttactagat cgaggtgtg 
      atagtcgtt tgtgctaat tatagagtt ggacctttc 
      ggttttctt tcgacaggc gatatgaca gtccctgga 
      aataacggt ctcaaagac cagctttta gccttgcag 
      tggacccac gacaatatt caccttttc ggtggtgat 
      ccaacaaaa gtaacaatt tttgggcaa agtgccggg 
      tctgcatcg gtcgcctac cacctctta cacacacag 
      tctcaaggg ttattccgg gccgttatt tgcgaaagt 
      ggttctttc ttgagtcct tgggcttac caaagaaat 
      gccagaaat tatgctttc accacagct ggtctaata 
      gacgaagtt gttggtaaa tcgaacgat tctcaaact 
      ttactcgac ttcttgcga agtgttagt gccaaagaa 
      ctggaccaa gcttcatac aaacttagt ttaacagag 
      gctcacaaa aataggcaa attcttcaa gggtttttc 
      ttcgcacct gttattgag ccaaagcat gatggggcg 
      ttcattagt caaaaaatg tacgtccag ttgaagaaa 
      ggtgatttt attagggtc ccaactttg atgggtttt 
      aattcagag gagaatatt tttcatact tatttgaaa 
      ttgtggttt gaaagtgcg ttaaacaca tatgataaa 
      aatatagac tggttagtg cctaatgat atgaggatc 
      aatgattcg gaaaaacga aaacaagtg ggaattaag 
      attagagat atttacact aatggaagc gatttactt 
      gaaaatgga ccttcctca gtcaggtat tacagcgat 
      acttcctac actaggcct atcaggaag catgcagaa 
      attcagtcg caattcagc gatgttttt ttttatcaa 
      ttctcttat gacggaaaa ttgggaaat tggtcattt 
      acagtgaaa ggcgctgag aatgtttca catggcgag 
      gacaattgt tacatttgg cgaaaatac gacgattat 
      tacgacaat tcagattta tgtcgtttt ccactacct 
      gaccgaata acacagtat cgcattcaa acaatatgg 
      accaatttt gcaaaatat ttgaaccct acaccaaag 
      aaaagcaag ttgcttcaa aacataaca tggccaaaa 
      tttgactcg aaagcacaa tatgtggat attttgaaa 
      aatttggaa attaagagt gagcccaaa aaagaaact 
      tacgaccaa tggattgaa ttgtacgat agtctggga 
      tacgacgat tttgacact tattaa
    mRNA sequence
    • >TC007922 RA
      ttagtattg gacatattt tgacaattt ttctccaga 
      tcatcgaac gcaattatt tcaccatag tacgatgaa 
      aatccatca gtgtaccgt ttccacctt cttaccctc 
      aattttagt gttgttttt tgtgaattt tggattcac 
      acaggatgc gcccattat caaattacc aaacggact 
      tattaaagg gcgtgtagg tcaaaccat agccaaacg 
      gccttactg ggcatacca gaaaatacc ttttgcaac 
      accacctct tggtgattt acgatttgc agcacctgt 
      tccctcaaa aaattggga tggggtttt ggagaccac 
      gaaatatga tgttatttg ttaccaaat caccagtga 
      ttcggattt agagtcgga agattgtct ttatttaaa 
      tgtgtacac tccgacaga cccttcaaa taaaacaaa 
      ccggggact tccagttat gttctttat ttatggggg 
      aggttttat tgaaggaaa ttgttttga ctatgtgta 
      cggtcctga atatttact agatcgagg tgtgatagt 
      cgtttgtgc taattatag agttggacc tttcggttt 
      tctttcgac aggcgatat gacagtccc tggaaataa 
      cggtctcaa agaccagct tttagcctt gcagtggac 
      ccacgacaa tattcacct tttcggtgg tgatccaac 
      aaaagtaac aatttttgg gcaaagtgc cgggtctgc 
      atcggtcgc ctaccacct cttacacac acagtctca 
      agggttatt ccgggccgt tatttgcga aagtggttc 
      tttcttgag tccttgggc ttaccaaag aaatgccag 
      aaattatgc tttcaccac agctggtct aatagacga 
      agttgttgg taaatcgaa cgattctca aactttact 
      cgacttctt gcgaagtgt tagtgccaa agaactgga 
      ccaagcttc atacaaact tagtttaac agaggctca 
      caaaaatag gcaaattct tcaagggtt tttcttcgc 
      acctgttat tgagccaaa gcatgatgg ggcgttcat 
      tagtcaaaa aatgtacgt ccagttgaa gaaaggtga 
      ttttattag ggtcccaac tttgatggg ttttaattc 
      agaggagaa tatttttca tacttattt gaaattgtg 
      gtttgaaag tgcgttaaa cacatatga taaaaatat 
      agactggtt agtgcctaa tgatatgag gatcaatga 
      ttcggaaaa acgaaaaca agtgggaat taagattag 
      agatattta cactaatgg aagcgattt acttgaaaa 
      tggaccttc ctcagtcag gtattacag cgatacttc 
      ctacactag gcctatcag gaagcatgc agaaattca 
      gtcgcaatt cagcgatgt tttttttta tcaattctc 
      ttatgacgg aaaattggg aaattggtc atttacagt 
      gaaaggcgc tgagaatgt ttcacatgg cgaggacaa 
      ttgttacat ttggcgaaa atacgacga ttattacga 
      caattcaga tttatgtcg ttttccact acctgaccg 
      aataacaca gtatcgcat tcaaacaat atggaccaa 
      ttttgcaaa atatttgaa ccctacacc aaagaaaag 
      caagttgct tcaaaacat aacatggcc aaaatttga 
      ctcgaaagc acaatatgt ggatatttt gaaaaattt 
      ggaaattaa gagtgagcc caaaaaaga aacttacga 
      ccaatggat tgaattgta cgatagtct gggatacga 
      cgattttga cacttatta atacaataa aaaaattat 
      ttgttattt tcttttatt tacttaata aaatattct 
      actttgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY