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Gene information
General information
TC007921 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2765144..2769750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007921
    iBeetle screen iB_04398

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: penetrance about 50 %
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages partially random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Adult morphology
    elytra surface irregular - Number of animals showing the phenotype: 8


    elytra sometimesnot closed - Number of animals showing the phenotype: 6


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007921 RA
      MLEETTIRE ILNKTQHTT SGHQAHIIR LTELYKNTP 
      HEFVPLFLK WLQFTIQRP SNIFVTRVL TFLSGFCAE 
      ITKIETKES GTNHFLTTI MSQVLKCSC LDDYNLRYN 
      SCYFISRVL QDIGGDVQL EVELIDEIQ DALLERMQE 
      NNVLIRLST MSALIRLQD PGNPECPVV EAFSDALCS 
      GSVNVRKEA VTHIAPTRQ TVDKLKDRV RDVDPHVRQ 
      TAFRRCGEI GAPYFRIVD RHMILTCGF KETHPTVKN 
      VFHNSLLPK WLNDYNSNY VRFIGALKL TADEEDVNN 
      LIDLSKKLM EEFYNKHSI HDLLAQLPI NADKLINTE 
      DLSNEVTLY WNILVELLR DSKNMDEHL ESIIPNLSP 
      FCDYIGRVI STKSAEQTD EWKNLEYQF DLLNLFEIA 
      ENYDYSDEV GRRTMHSLT NKLLQSHRF TPKLTKKLV 
      SLAHKCSSN VEFFINDMC QVISEIREP LTEQPLSQD 
      QQREHAFQA ARLRVRINI LHEEQNEAI EKRDYEKAQ 
      FLENELTKC KNQLEEMQN QINVEKARV VKSDPDTLC 
      RCLDILASV LSLDAVTKL TPSLVACKE NFLEPLIKN 
      PNPDIHWRI IKCLGLFSF VDKQTADVY AKIVSIPIA 
      TYRSVEIRN IAALVQSMM ATTDLVTKY GIEIISGED 
      LSDSASTSD QDSQKRKLY LADNDKQVS CKIDKITLP 
      YLLEIFLDF LDDESDEIR YQAVQLLAI LVQRFPLTP 
      NVLSRILLK WFNPLTGKF DSRLQIALS WIINELNGN 
      KQFDEIVKE SIVPTLTTI IKAPRTSPL VDIDTDNVI 
      KFFAEIMNT TVSKSIRKN LAIDVCIKI AENRKDRIV 
      PLLIKLLNL LELSQEEDL EEVIQRTEA LLEALEDKV 
      LQRLLRKFI DKYQKLNNE NNNGNEIQE PNASREISP 
      TSEDNNPEV SRITRQKKR VSKECRVLL EKLNLSTPA 
      NETTLHTIY EASMEMEVD NSLNIETPT TSSKSSSGR 
      SSTLVVSAT SESDDREVV RRQSTRRRK RRKI
    CDS sequence
    • >TC007921 RA
      atgctggag gaaacgacc atacgtgaa atattaaac 
      aagacgcaa cacaccact agtggtcat caagcacat 
      atcatacgg ttaacggag ctgtataaa aatacgccc 
      cacgagttt gttcctcta tttttgaaa tggctgcaa 
      tttaccatt cagagacct tcaaacatc ttcgttaca 
      cgagtcttg acttttctg tctggcttt tgcgccgaa 
      attaccaaa attgaaaca aaagagagc ggaactaat 
      cacttctta accacgata atgagccaa gtacttaag 
      tgcagttgt ttggacgat tacaattta agatacaac 
      tcttgctac tttatttca agagtcctg caggatatt 
      ggcggagat gtccaacta gaagtggaa ctgattgat 
      gaaattcag gacgcgtta cttgaacgg atgcaggaa 
      aacaatgta ctcattcgt ttatcaaca atgtcagct 
      ttgatcagg ctgcaagac cctggaaac cccgaatgt 
      cccgtagtt gaagccttt tcggatgct ttatgcagt 
      ggcagtgta aacgtgcgg aaagaagca gtcactcat 
      atcgcacca actcggcag actgtagac aaattaaaa 
      gatagagtt agagatgtc gatccacat gtgcgccaa 
      actgccttc cgccgatgt ggcgagata ggggcgccc 
      tattttcga atcgtggat agacacatg attttaacg 
      tgcggattt aaagaaaca catccaact gtgaaaaat 
      gtgtttcat aattctttg ttaccaaaa tggttaaat 
      gactataac agtaactat gtgcggttt attggcgct 
      ttaaaactc actgcggac gaagaagat gtgaataac 
      ctgattgat ttgagcaaa aaattgatg gaggaattt 
      tacaataaa cattcgatt cacgatctt ttggcacag 
      ttgccaatc aatgcagat aaactgatt aacactgaa 
      gacctatca aatgaagtc actctctac tggaatatt 
      ttagttgag cttttgcgc gactctaaa aatatggat 
      gaacacctg gagagtatc atcccaaac ttatctccc 
      ttttgtgac tacattgga agagtaatt tctacaaaa 
      tctgctgag caaacggac gaatggaaa aacctggag 
      taccagttt gacctgtta aatttgttc gagattgcg 
      gaaaattac gattactcc gatgaggtc ggcagacgc 
      acgatgcac tcgctcaca aacaaactg ttacaaagc 
      cacagattt acgccaaaa ttaacgaaa aaactggtt 
      tcgctcgcg cacaagtgc agcagcaac gttgagttt 
      ttcataaat gatatgtgc caagtcatt tctgaaata 
      agagaaccg ttaaccgaa caacctttg tcgcaggac 
      caacagagg gaacatgca tttcaagcc gcgagattg 
      agggtgcga atcaacatc ttgcatgaa gaacagaac 
      gaagcaatt gagaaacgt gactatgaa aaggcccaa 
      tttctcgag aacgagtta accaagtgc aagaaccaa 
      ttggaagag atgcagaat caaattaat gtcgaaaaa 
      gctcgagtt gttaagtca gatccggac acgttgtgt 
      cgctgtttg gatattctg gcgagcgtt ctttcattg 
      gatgcagta actaaactg acgccgtca ttggttgcg 
      tgtaaggag aattttttg gaaccgttg attaaaaac 
      ccaaatccg gacatacat tggcggatt atcaagtgt 
      ttggggctg ttttcgttc gtcgataag caaacggct 
      gatgtgtac gctaaaatt gtttctatt cctatcgct 
      acttatcgt tcggttgaa atacgtaac attgctgca 
      ctggttcag tcaatgatg gcaacaacc gatttagtg 
      accaagtac ggtatcgaa attataagt ggtgaagac 
      ttgtcagat tctgcgtca acttctgat caagattcc 
      caaaaacga aagctttat ttagccgac aacgacaag 
      caagtatct tgcaaaatc gacaaaata acacttcca 
      tatcttctt gaaatattt ttggacttt ttggatgat 
      gagagtgat gaaattcgg taccaagcc gttcaatta 
      ttagcgatt ctggttcaa agatttccg ttaactcca 
      aatgtgctt tctaggatt ttgcttaag tggtttaat 
      ccattaacg gggaaattc gatagtcgt ttgcaaatt 
      gctttgagc tggataatt aacgagctg aatgggaac 
      aaacagttt gatgaaatt gtgaaagag tcaattgtg 
      ccgacacta accaccata attaaggca ccaaggact 
      agtcctttg gtggacatt gataccgat aacgttata 
      aagtttttc gccgaaata atgaacacc actgtgtcg 
      aaaagcatt cgtaaaaat ttagcgata gatgtgtgc 
      ataaaaata gcggaaaac cggaaagac cgcatcgtt 
      ccacttctt ataaagttg ttgaatctt ttagaactg 
      agtcaagag gaggatttg gaagaagtt atacaacga 
      actgaagct cttttagag gccctggaa gataaagta 
      ttacagagg cttctaaga aaatttatt gataaatac 
      caaaaattg aacaatgag aataataac ggaaatgaa 
      atacaagaa ccaaacgct tcacgagaa atttcaccc 
      actagtgaa gacaacaat ccagaagtg tcccgaatt 
      acaagacaa aaaaagcgt gtcagcaaa gagtgtagg 
      gttctttta gaaaagtta aatttgtca actccagct 
      aatgaaaca actctccac acaatttat gaggcgagt 
      atggagatg gaggtggac aactcttta aacattgaa 
      actccgaca acatcttca aaaagttcg agtggccga 
      agcagtact ctagtggtt tctgcaacg tccgagtcc 
      gatgatcgt gaagtcgtc cgaaggcag tcaacgaga 
      cgtagaaaa aggagaaaa atataa
    mRNA sequence
    • >TC007921 RA
      ttcaagaaa actttaaaa atttgacat ctgacagaa 
      agcgtacta tgctagcgt catttgcgt aaatggtcg 
      tttattttc attccaatt tgacttgtt tggtgattt 
      gtgccactg cagtttgaa tgtgagggt aatttgtga 
      tgtgtgtgt gcgttttaa ataaaatgc tggaggaaa 
      cgaccatac gtgaaatat taaacaaga cgcaacaca 
      ccactagtg gtcatcaag cacatatca tacggttaa 
      cggagctgt ataaaaata cgccccacg agtttgttc 
      ctctatttt tgaaatggc tgcaattta ccattcaga 
      gaccttcaa acatcttcg ttacacgag tcttgactt 
      ttctgtctg gcttttgcg ccgaaatta ccaaaattg 
      aaacaaaag agagcggaa ctaatcact tcttaacca 
      cgataatga gccaagtac ttaagtgca gttgtttgg 
      acgattaca atttaagat acaactctt gctacttta 
      tttcaagag tcctgcagg atattggcg gagatgtcc 
      aactagaag tggaactga ttgatgaaa ttcaggacg 
      cgttacttg aacggatgc aggaaaaca atgtactca 
      ttcgtttat caacaatgt cagctttga tcaggctgc 
      aagaccctg gaaaccccg aatgtcccg tagttgaag 
      ccttttcgg atgctttat gcagtggca gtgtaaacg 
      tgcggaaag aagcagtca ctcatatcg caccaactc 
      ggcagactg tagacaaat taaaagata gagttagag 
      atgtcgatc cacatgtgc gccaaactg ccttccgcc 
      gatgtggcg agatagggg cgccctatt ttcgaatcg 
      tggatagac acatgattt taacgtgcg gatttaaag 
      aaacacatc caactgtga aaaatgtgt ttcataatt 
      ctttgttac caaaatggt taaatgact ataacagta 
      actatgtgc ggtttattg gcgctttaa aactcactg 
      cggacgaag aagatgtga ataacctga ttgatttga 
      gcaaaaaat tgatggagg aattttaca ataaacatt 
      cgattcacg atcttttgg cacagttgc caatcaatg 
      cagataaac tgattaaca ctgaagacc tatcaaatg 
      aagtcactc tctactgga atattttag ttgagcttt 
      tgcgcgact ctaaaaata tggatgaac acctggaga 
      gtatcatcc caaacttat ctccctttt gtgactaca 
      ttggaagag taatttcta caaaatctg ctgagcaaa 
      cggacgaat ggaaaaacc tggagtacc agtttgacc 
      tgttaaatt tgttcgaga ttgcggaaa attacgatt 
      actccgatg aggtcggca gacgcacga tgcactcgc 
      tcacaaaca aactgttac aaagccaca gatttacgc 
      caaaattaa cgaaaaaac tggtttcgc tcgcgcaca 
      agtgcagca gcaacgttg agtttttca taaatgata 
      tgtgccaag tcatttctg aaataagag aaccgttaa 
      ccgaacaac ctttgtcgc aggaccaac agagggaac 
      atgcatttc aagccgcga gattgaggg tgcgaatca 
      acatcttgc atgaagaac agaacgaag caattgaga 
      aacgtgact atgaaaagg cccaatttc tcgagaacg 
      agttaacca agtgcaaga accaattgg aagagatgc 
      agaatcaaa ttaatgtcg aaaaagctc gagttgtta 
      agtcagatc cggacacgt tgtgtcgct gtttggata 
      ttctggcga gcgttcttt cattggatg cagtaacta 
      aactgacgc cgtcattgg ttgcgtgta aggagaatt 
      ttttggaac cgttgatta aaaacccaa atccggaca 
      tacattggc ggattatca agtgtttgg ggctgtttt 
      cgttcgtcg ataagcaaa cggctgatg tgtacgcta 
      aaattgttt ctattccta tcgctactt atcgttcgg 
      ttgaaatac gtaacattg ctgcactgg ttcagtcaa 
      tgatggcaa caaccgatt tagtgacca agtacggta 
      tcgaaatta taagtggtg aagacttgt cagattctg 
      cgtcaactt ctgatcaag attcccaaa aacgaaagc 
      tttatttag ccgacaacg acaagcaag tatcttgca 
      aaatcgaca aaataacac ttccatatc ttcttgaaa 
      tatttttgg actttttgg atgatgaga gtgatgaaa 
      ttcggtacc aagccgttc aattattag cgattctgg 
      ttcaaagat ttccgttaa ctccaaatg tgctttcta 
      ggattttgc ttaagtggt ttaatccat taacgggga 
      aattcgata gtcgtttgc aaattgctt tgagctgga 
      taattaacg agctgaatg ggaacaaac agtttgatg 
      aaattgtga aagagtcaa ttgtgccga cactaacca 
      ccataatta aggcaccaa ggactagtc ctttggtgg 
      acattgata ccgataacg ttataaagt ttttcgccg 
      aaataatga acaccactg tgtcgaaaa gcattcgta 
      aaaatttag cgatagatg tgtgcataa aaatagcgg 
      aaaaccgga aagaccgca tcgttccac ttcttataa 
      agttgttga atcttttag aactgagtc aagaggagg 
      atttggaag aagttatac aacgaactg aagctcttt 
      tagaggccc tggaagata aagtattac agaggcttc 
      taagaaaat ttattgata aataccaaa aattgaaca 
      atgagaata ataacggaa atgaaatac aagaaccaa 
      acgcttcac gagaaattt cacccacta gtgaagaca 
      acaatccag aagtgtccc gaattacaa gacaaaaaa 
      agcgtgtca gcaaagagt gtagggttc ttttagaaa 
      agttaaatt tgtcaactc cagctaatg aaacaactc 
      tccacacaa tttatgagg cgagtatgg agatggagg 
      tggacaact ctttaaaca ttgaaactc cgacaacat 
      cttcaaaaa gttcgagtg gccgaagca gtactctag 
      tggtttctg caacgtccg agtccgatg atcgtgaag 
      tcgtccgaa ggcagtcaa cgagacgta gaaaaagga 
      gaaaaatat aaatgattt tgtacatac atattttct 
      agttgatta agttttgca gtttcaagc tgtaaagaa 
      cattggagt actctgtcg tcttaacct tcagcttta 
      ctttactcc aagtcagat taaaaaaaa gatgagagg 
      gtataagta attttgtat atttatatt tactcctat 
      tacgagtgt acatgtttt ctagaataa ttattaaat 
      ttattacca acgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY