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Gene information
General information
TC007916 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2656673..2658699
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007916
    iBeetle screen iB_04397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007916 RA
      MNFLFNSRL GFNPLGHPR RTHLRPLIR CQHKIQQRR 
      LQKHHLTES PHKIIPGLS GLAATNRRR LHLPPSSLG 
      VSLPELSNV FPQCLTTRH RRSIIILIP EKTPIEKVA 
      PDDRRGSSS RQLFDDDML VPPDMLAAQ SKMVYQLNK 
      YYTEKVQAR KLQVSKTIQ EVCRVVQDV LKEVEVQEP 
      RFISSLTDY NGRYDGLEV ISPVEFEVV IYLNQMGVL 
      NFVDDGTLP GCAVLKLSD GRKRSMSLW VEFITASGY 
      LSARKMRSR FQTLVAQAC DKCSYRDSV KMIADTTEV 
      KLRIRERFI VQITPAFKC AGLWPRSAA HWPLPQIPW 
      PHPNLVVEV KTEGFDLLS KECIALQGK QSAMEGDAW 
      VLSFIEAEN RLLQGGCRK RCLSILKTL RDRHLDLPG 
      NPVTSYHMK TLLLYECEK HPRETEWDE ACIADRING 
      IFLQLISCL QCKRCPHYF LPNLDLFKG KSPSALENA 
      AKQVWRLTR EMLTNTRCF EKL
    CDS sequence
    • >TC007916 RA
      atgaatttc ctatttaat agcagactc ggatttaac 
      ccccttggc cacccccgc cgcacccat cttcgtcca 
      ttaatccgt tgtcaacac aaaattcaa caacgccgc 
      ttacaaaag caccattta accgaatcg cctcataaa 
      ataatcccc ggactttcc ggattggcc gccacgaat 
      aggcggcgc ttgcacctc ccgccctcc tctttgggt 
      gtgtcttta cctgaactc tccaacgtt ttccctcag 
      tgtctcacg actcgtcat cgtcgatcc atcatcatc 
      ctcatcccg gaaaaaaca cccatcgaa aaagtggct 
      ccggatgat cggcgcggc tccagtagc cgacaactc 
      ttcgacgac gacatgctg gtgccgcct gacatgctg 
      gcggcacaa tccaagatg gtctaccaa ctcaacaaa 
      tactacacg gaaaaagtc caagcccgc aaactacaa 
      gtcagcaag acaatacaa gaggtgtgc cgagtggtc 
      caggacgtc ctcaaggaa gtggaagtg caagaaccg 
      aggtttatc tcatctttg acggactat aacgggcgc 
      tatgacggc ctggaagtc atctcgccg gtcgagttt 
      gaagtcgtg atatatctc aatcagatg ggagtgctc 
      aacttcgtc gacgacggg actctaccc ggctgtgcg 
      gtcctcaag ctgagcgac ggaaggaaa cgctcgatg 
      agcctctgg gtcgagttc atcacagcc tccggctac 
      ttatcagcc cggaaaatg cgctccagg ttccaaacc 
      ctcgtggct caagcctgt gacaagtgt tcttatcgc 
      gactccgtg aagatgatc gccgacacc accgaagtc 
      aaactccgg attcgcgag cgttttatc gtccaaatc 
      acgccggcg ttcaaatgt gccggtcta tggccccga 
      tctgcggcc cactggccg ctcccccaa atcccctgg 
      ccgcacccg aacctcgtc gtcgaagtg aaaaccgaa 
      ggcttcgac cttctgtcc aaagaatgc atcgcattg 
      caaggcaaa caatccgcc atggaaggc gacgcctgg 
      gtcctctcc ttcatcgag gccgaaaac cgcctcctg 
      caaggcggc tgccggaag cgctgtctc agcatcctc 
      aaaaccctg cgggacaga cacctggac ctgcccgga 
      aaccccgtc accagctac cacatgaaa accctcctc 
      ctgtacgaa tgcgagaaa cacccccgg gagacggaa 
      tgggacgag gcgtgcatt gccgaccga atcaacggg 
      atcttcctg cagttaatc tcgtgtcta cagtgcaaa 
      aggtgcccg cattacttc ctgccaaac ctcgacttg 
      ttcaaagga aaatcgccg agcgcgctc gaaaacgcc 
      gccaaacaa gtctggaga ctcacccga gagatgtta 
      acgaacact aggtgtttc gaaaagttg tag
    mRNA sequence
    • >TC007916 RA
      agtttgatg acttttatt ttgtttaac atgaatttc 
      ctatttaat agcagactc ggatttaac ccccttggc 
      cacccccgc cgcacccat cttcgtcca ttaatccgt 
      tgtcaacac aaaattcaa caacgccgc ttacaaaag 
      caccattta accgaatcg cctcataaa ataatcccc 
      ggactttcc ggattggcc gccacgaat aggcggcgc 
      ttgcacctc ccgccctcc tctttgggt gtgtcttta 
      cctgaactc tccaacgtt ttccctcag tgtctcacg 
      actcgtcat cgtcgatcc atcatcatc ctcatcccg 
      gaaaaaaca cccatcgaa aaagtggct ccggatgat 
      cggcgcggc tccagtagc cgacaactc ttcgacgac 
      gacatgctg gtgccgcct gacatgctg gcggcacaa 
      tccaagatg gtctaccaa ctcaacaaa tactacacg 
      gaaaaagtc caagcccgc aaactacaa gtcagcaag 
      acaatacaa gaggtgtgc cgagtggtc caggacgtc 
      ctcaaggaa gtggaagtg caagaaccg aggtttatc 
      tcatctttg acggactat aacgggcgc tatgacggc 
      ctggaagtc atctcgccg gtcgagttt gaagtcgtg 
      atatatctc aatcagatg ggagtgctc aacttcgtc 
      gacgacggg actctaccc ggctgtgcg gtcctcaag 
      ctgagcgac ggaaggaaa cgctcgatg agcctctgg 
      gtcgagttc atcacagcc tccggctac ttatcagcc 
      cggaaaatg cgctccagg ttccaaacc ctcgtggct 
      caagcctgt gacaagtgt tcttatcgc gactccgtg 
      aagatgatc gccgacacc accgaagtc aaactccgg 
      attcgcgag cgttttatc gtccaaatc acgccggcg 
      ttcaaatgt gccggtcta tggccccga tctgcggcc 
      cactggccg ctcccccaa atcccctgg ccgcacccg 
      aacctcgtc gtcgaagtg aaaaccgaa ggcttcgac 
      cttctgtcc aaagaatgc atcgcattg caaggcaaa 
      caatccgcc atggaaggc gacgcctgg gtcctctcc 
      ttcatcgag gccgaaaac cgcctcctg caaggcggc 
      tgccggaag cgctgtctc agcatcctc aaaaccctg 
      cgggacaga cacctggac ctgcccgga aaccccgtc 
      accagctac cacatgaaa accctcctc ctgtacgaa 
      tgcgagaaa cacccccgg gagacggaa tgggacgag 
      gcgtgcatt gccgaccga atcaacggg atcttcctg 
      cagttaatc tcgtgtcta cagtgcaaa aggtgcccg 
      cattacttc ctgccaaac ctcgacttg ttcaaagga 
      aaatcgccg agcgcgctc gaaaacgcc gccaaacaa 
      gtctggaga ctcacccga gagatgtta acgaacact 
      aggtgtttc gaaaagttg tagtgttag tgtttctta 
      cgaagaatt ctcaaactg tgatttacc gcttaagag 
      aacaaaata tattattat tggtatgtt tttaataaa 
      aagcgaaac taatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY