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Gene information
General information
TC007913 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2626003..2631597
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007913
    iBeetle screen iB_04395

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007913 RA
      MRCEINKNL ITLKCVLFL FFGALGSLF PFLPNHMNG 
      IGLSRDEFT IISIVSPLV AVIGPLVAA PLADRLAGG 
      YGGSPRSKT GRYLRVMIS VCLGLAIIL YWLLLTVPR 
      IQRSPSSVT FTCNEDGAF VLQNKCGHE RTCHSWNGE 
      KGEISLRDC TFSCNATTL YTGVPPPEP SPQTKENDT 
      TTEDYYDDA NPANEAEPD FGTTIIPVS NSKANPGIV 
      LSPHLCHKT DKDVTICDV YTKFSKTIQ MPLSLKSTP 
      ATSSSNETE SEDDDICRY PVADYFHCR MPQEIVKEL 
      KATEDPYCQ PIVLCAIHE PYTSASLLK TSECGYNAM 
      SFWLYLVIR SIADIFPAA AVALLSTAV VIATRETST 
      GRSDIGKQL AAGALGFAI FAPIIGGCA NGNLRDALI 
      CFTVLNLLA IVILLFDNN MPLSPPEWW WYTRCGLLA 
      LPMSSVRKY RNEVIALVV VLFVLGILW NAIDAYLPW 
      NAATMPDSS NLIIGLTVT MGAIPAISL LTFAEKVVD 
      YCSHSNILI LCFVTYVVH YVALANITE AAVLLIFEV 
      LEIFTLHLM WVTAILYLR HLVPRKYTA LGQALPVIA 
      HFCLGRCFG ALIGRFAYT LYPEKVKYP DNHGPVYGG 
      LAIAAAIIA ALYFVAYHF YLKPYCVPH VQLPPYPAP 
      SVVQSVNGN GSYTPLRVY HNGRAKKGH FRY
    CDS sequence
    • >TC007913 RA
      atgagatgc gaaatcaac aaaaaccta atcacactg 
      aaatgcgtc ctgttcctg tttttcggg gctttgggg 
      agtctcttc ccctttttg cccaaccac atgaacggg 
      attggcctg tcccgggac gaattcacc atcatttcg 
      atcgtctcg cctctagtt gcggtaatc gggcctttg 
      gtggccgcc cccttagct gatcgactt gcagggggg 
      tatggaggc tcgcccagg agcaagaca gggaggtat 
      ctccgggtc atgatttcg gtgtgtctg ggacttgcc 
      ataatcctc tactggctt ctactgaca gtgccaagg 
      atccaacgg agtccttca agtgttacg ttcacgtgt 
      aatgaggat ggcgccttc gtccttcag aataaatgt 
      gggcatgag aggacgtgc catagctgg aatggggaa 
      aaaggagaa atttccttg cgggattgt acgttttcc 
      tgcaacgcc acgactctc tatactggg gttccgcca 
      ccggaaccg tcaccccag actaaggaa aacgacacc 
      acaacggaa gactattac gacgatgcc aaccctgct 
      aatgaagct gaacctgat tttggtact actataatc 
      ccggtcagt aacagcaag gccaatcct gggattgtc 
      ctcagcccc cacttatgc cacaagact gacaaagat 
      gtaacaatt tgtgatgtc tataccaaa ttttccaaa 
      acaatacaa atgccccta agtctgaaa tcgacccct 
      gccacctcc agtagcaac gaaactgaa tcagaagac 
      gacgatatt tgtcgatac ccagttgcc gattatttc 
      cactgcagg atgcctcag gaaatcgtc aaagagttg 
      aaagcgacc gaggaccct tattgccaa cccatcgtc 
      ctgtgtgca atccacgag ccgtacact tccgccagt 
      ttactcaaa acttccgaa tgcggttac aacgccatg 
      agtttttgg ctgtatctg gtcatacga tcaatcgct 
      gatattttc cccgccgca gctgtggcc cttctgagc 
      actgctgtg gtgatagcg actcgggag acttccacc 
      ggccggagc gacatcggc aaacaacta gccgctggc 
      gctctaggt tttgccatt ttcgccccg attatcggc 
      gggtgcgct aatggaaac cttagagac gccctaatc 
      tgcttcact gtgcttaat ctgctcgcg attgtgatt 
      ttattgttc gataataac atgccgttg agtccgccc 
      gaatggtgg tggtacacc cgctgtggc cttctggcg 
      cttcccatg tcttcggtc cggaagtac cgcaacgag 
      gtgatcgcc cttgtggtc gttctgttc gttttgggg 
      attctctgg aacgcgatt gacgcctac ttgccgtgg 
      aacgcggcc acaatgccc gacagctcg aaccttata 
      attgggctg acggtgacg atgggcgcg atcccggcc 
      atttccctg ctgactttc gccgaaaaa gtggtcgat 
      tattgcagc cactccaac attcttatc ttgtgtttc 
      gtcacttat gtcgtccat tacgtggcc ttggcgaat 
      ataacggaa gctgccgtt ttgctcatc tttgaagtt 
      ttggagatt tttacgctg catctcatg tgggttact 
      gccatactt tacttaaga cacttggtt ccgagaaaa 
      tacactgcc ttgggacaa gcactgccc gtcattgct 
      catttctgc ttaggccgt tgcttcggc gctttgatc 
      ggccgattc gcctacacc ctttatccg gagaaggtg 
      aaatatccg gacaaccac ggccccgtc tacgggggt 
      ctagctatc gcggctgca atcatagca gctctctat 
      ttcgtagca tatcacttc tacctgaag ccctattgt 
      gtgccgcat gttcaactc ccgccctat ccggcacct 
      tcggttgtc caaagtgtg aatgggaac gggtcgtat 
      acccctctc agagtgtat cacaacgga agggcgaag 
      aaagggcac tttagatat tag
    mRNA sequence
    • >TC007913 RA
      ctccaacgg caaacatgt ggtgtatag agtgcggct 
      gactggagt ttctcatac aacttcgca tttacacac 
      tagttgctg gcgcaactt tgtcgagga aagtcgatt 
      caaacgatt atttcgacg ttcacgtgg agtggtttt 
      atttattta gatcggcga accgggtcg atggtttag 
      tttgtgttt ggcgatttg gatttaatt cgagcgtga 
      ttttttgat ctctccgac tcatgaatc gaaaagtaa 
      aatgccagc cagatagaa ccaagcagc caaaatgag 
      atgcgaaat caacaaaaa cctaatcac actgaaatg 
      cgtcctgtt cctgttttt cggggcttt ggggagtct 
      cttcccctt tttgcccaa ccacatgaa cgggattgg 
      cctgtcccg ggacgaatt caccatcat ttcgatcgt 
      ctcgcctct agttgcggt aatcgggcc tttggtggc 
      cgccccctt agctgatcg acttgcagg ggggtatgg 
      aggctcgcc caggagcaa gacagggag gtatctccg 
      ggtcatgat ttcggtgtg tctgggact tgccataat 
      cctctactg gcttctact gacagtgcc aaggatcca 
      acggagtcc ttcaagtgt tacgttcac gtgtaatga 
      ggatggcgc cttcgtcct tcagaataa atgtgggca 
      tgagaggac gtgccatag ctggaatgg ggaaaaagg 
      agaaatttc cttgcggga ttgtacgtt ttcctgcaa 
      cgccacgac tctctatac tggggttcc gccaccgga 
      accgtcacc ccagactaa ggaaaacga caccacaac 
      ggaagacta ttacgacga tgccaaccc tgctaatga 
      agctgaacc tgattttgg tactactat aatcccggt 
      cagtaacag caaggccaa tcctgggat tgtcctcag 
      cccccactt atgccacaa gactgacaa agatgtaac 
      aatttgtga tgtctatac caaattttc caaaacaat 
      acaaatgcc cctaagtct gaaatcgac ccctgccac 
      ctccagtag caacgaaac tgaatcaga agacgacga 
      tatttgtcg atacccagt tgccgatta tttccactg 
      caggatgcc tcaggaaat cgtcaaaga gttgaaagc 
      gaccgagga cccttattg ccaacccat cgtcctgtg 
      tgcaatcca cgagccgta cacttccgc cagtttact 
      caaaacttc cgaatgcgg ttacaacgc catgagttt 
      ttggctgta tctggtcat acgatcaat cgctgatat 
      tttccccgc cgcagctgt ggcccttct gagcactgc 
      tgtggtgat agcgactcg ggagacttc caccggccg 
      gagcgacat cggcaaaca actagccgc tggcgctct 
      aggttttgc cattttcgc cccgattat cggcgggtg 
      cgctaatgg aaaccttag agacgccct aatctgctt 
      cactgtgct taatctgct cgcgattgt gattttatt 
      gttcgataa taacatgcc gttgagtcc gcccgaatg 
      gtggtggta cacccgctg tggccttct ggcgcttcc 
      catgtcttc ggtccggaa gtaccgcaa cgaggtgat 
      cgcccttgt ggtcgttct gttcgtttt ggggattct 
      ctggaacgc gattgacgc ctacttgcc gtggaacgc 
      ggccacaat gcccgacag ctcgaacct tataattgg 
      gctgacggt gacgatggg cgcgatccc ggccatttc 
      cctgctgac tttcgccga aaaagtggt cgattattg 
      cagccactc caacattct tatcttgtg tttcgtcac 
      ttatgtcgt ccattacgt ggccttggc gaatataac 
      ggaagctgc cgttttgct catctttga agttttgga 
      gatttttac gctgcatct catgtgggt tactgccat 
      actttactt aagacactt ggttccgag aaaatacac 
      tgccttggg acaagcact gcccgtcat tgctcattt 
      ctgcttagg ccgttgctt cggcgcttt gatcggccg 
      attcgccta cacccttta tccggagaa ggtgaaata 
      tccggacaa ccacggccc cgtctacgg gggtctagc 
      tatcgcggc tgcaatcat agcagctct ctatttcgt 
      agcatatca cttctacct gaagcccta ttgtgtgcc 
      gcatgttca actcccgcc ctatccggc accttcggt 
      tgtccaaag tgtgaatgg gaacgggtc gtatacccc 
      tctcagagt gtatcacaa cggaagggc gaagaaagg 
      gcactttag atattagct atttatgat actctgtgt 
      aaaaggagc tttacgtaa ctgtaaaat atttatccg 
      cgaggcttt agccccatt ttttgtaca tttcgcagc 
      tttaatata atattaata tattgattg aatgtttta 
      tatatgaaa tgtgtacat cgtgctttt tcttgttgt 
      tttgtaatt gtcacgttt tattgtttt gtaacgttt 
      tgcaattca ctccaatat ggatatcgc aactttgta 
      taaatggat attttttta atgaaatta ataaattat 
      taaagtagc a
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