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Gene information
General information
TC007874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2026969..2028699
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007874
    iBeetle screen iB_04392

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007874 RA
      MTVTYTKEV ATCRGFSCF LRLLLKWKG SIYKLVYKE 
      LLVFLFIYY FLNLTYKYI LNRTGRDVF IQIVHYCNA 
      STNYIPLSF VLGFYVSIV MARWWDQYR SIPFPDSTA 
      VYVSATIHG QDERGRVMR RTIMRYVCL SVTMLFTNI 
      SPKVKKRFP TLDHLMKAG LLEESEKKV IQGLNQKFP 
      SYSKHWLPL VWASSIVTR ARKEGRIRD DFAVKTMLD 
      EINAFRGKC GILINYDRI SIPLVYTQV VTLAVYTYF 
      LSALMGHQC VEGHGYVFD KYFPLFTLL QFFFYMGWL 
      KVAETLINP FGDDDDDFE LNWMVDRNL QISYLIVDE 
      MHHEHPELI KDYFWDQVL PEELPYTPE TEQYREMHP 
      LPSTANLVS KHEDDLIGG SSYRIFGSS NTLRKRSNS 
      VVSLLRKIV RSDSTSNES EQEMWEKIP RTKLEEVIE 
      EVDEQLTIM SSRENDLKR PRIQDIFGP TTQPLDSPQ 
      EESPSFDSL KQKREEERL RRFRRNLAN YLEHKEPS
    CDS sequence
    • >TC007874 RA
      atgactgtt acgtatacg aaagaagtt gcaacttgt 
      cgtggcttc tcctgcttt ctaagactg ctactaaaa 
      tggaagggc agtatttac aagctcgtg tacaaagaa 
      ctgctagtg ttcttgttc atttactac ttcttaaat 
      ctcacgtat aagtacatc ctgaacagg accggtcgc 
      gatgttttt atccagatc gtgcactat tgcaacgct 
      agcactaat tacatcccc ctgtctttc gttttgggt 
      ttctatgtc tcaatagtg atggcacgt tggtgggac 
      caataccgc tctattccc ttcccggac tccactgcg 
      gtttacgtg agcgcaaca atccacggc caggacgaa 
      cgtggccgg gttatgcgg cggacaata atgcgctac 
      gtttgcctc tccgtcaca atgctcttc accaacatc 
      tcccccaaa gtcaaaaag cgcttccca acgcttgac 
      cacttgatg aaagccggt ctgcttgag gaaagcgag 
      aagaaagtg atccaagga ttgaaccaa aagttccct 
      tcgtactcc aaacactgg ctgccttta gtctgggct 
      tccagcatt gtgacccga gcccggaag gaaggccgc 
      attcgggac gatttcgcc gtcaagaca atgcttgac 
      gagatcaat gccttccgg ggcaagtgc ggtattttg 
      atcaattat gacaggatc agcatcccg ctggtgtac 
      acccaagtg gtgactttg gccgtctac acttacttc 
      ctctcggct ctgatgggg caccagtgc gtggaaggg 
      cacggctac gtgttcgat aaatacttc ccgctgttc 
      acgctcctg cagtttttc ttctacatg gggtggttg 
      aaagtcgcg gagactctg atcaatccg ttcggtgat 
      gacgacgac gattttgaa ctgaactgg atggtggac 
      agaaacttg caaatttcg tatttgatt gtggacgaa 
      atgcaccat gagcacccg gaactcatc aaggactat 
      ttctgggac caggtgctt ccggaggag ttgccctac 
      acccccgag acggaacag taccgcgaa atgcacccg 
      ttaccctcg actgcgaat ttagtgtcc aagcatgag 
      gacgatctt atcgggggt tcgtcctac cgaattttc 
      ggttcgtct aacacttta cgcaagcga tcgaactcg 
      gttgtgagt cttttgcgc aaaattgtg cgaagtgat 
      agcacttca aacgagtcg gagcaggag atgtgggag 
      aaaataccg cggactaag ctcgaggaa gttatcgaa 
      gaggtggac gagcaactg acaattatg tcttcgcgt 
      gagaacgat ttgaagcgg ccccgaatt caggatatt 
      ttcggaccg acgacgcaa ccccttgat agtccccag 
      gaggaatct ccgtcgttc gattcgttg aaacagaag 
      cgtgaagaa gagagattg cggaggttt aggcgtaat 
      ctggccaac tatctcgag cacaaggag ccatcgtga
    mRNA sequence
    • >TC007874 RA
      agctaaaca aatatgact gttacgtat acgaaagaa 
      gttgcaact tgtcgtggc ttctcctgc tttctaaga 
      ctgctacta aaatggaag ggcagtatt tacaagctc 
      gtgtacaaa gaactgcta gtgttcttg ttcatttac 
      tacttctta aatctcacg tataagtac atcctgaac 
      aggaccggt cgcgatgtt tttatccag atcgtgcac 
      tattgcaac gctagcact aattacatc cccctgtct 
      ttcgttttg ggtttctat gtctcaata gtgatggca 
      cgttggtgg gaccaatac cgctctatt cccttcccg 
      gactccact gcggtttac gtgagcgca acaatccac 
      ggccaggac gaacgtggc cgggttatg cggcggaca 
      ataatgcgc tacgtttgc ctctccgtc acaatgctc 
      ttcaccaac atctccccc aaagtcaaa aagcgcttc 
      ccaacgctt gaccacttg atgaaagcc ggtctgctt 
      gaggaaagc gagaagaaa gtgatccaa ggattgaac 
      caaaagttc ccttcgtac tccaaacac tggctgcct 
      ttagtctgg gcttccagc attgtgacc cgagcccgg 
      aaggaaggc cgcattcgg gacgatttc gccgtcaag 
      acaatgctt gacgagatc aatgccttc cggggcaag 
      tgcggtatt ttgatcaat tatgacagg atcagcatc 
      ccgctggtg tacacccaa gtggtgact ttggccgtc 
      tacacttac ttcctctcg gctctgatg gggcaccag 
      tgcgtggaa gggcacggc tacgtgttc gataaatac 
      ttcccgctg ttcacgctc ctgcagttt ttcttctac 
      atggggtgg ttgaaagtc gcggagact ctgatcaat 
      ccgttcggt gatgacgac gacgatttt gaactgaac 
      tggatggtg gacagaaac ttgcaaatt tcgtatttg 
      attgtggac gaaatgcac catgagcac ccggaactc 
      atcaaggac tatttctgg gaccaggtg cttccggag 
      gagttgccc tacaccccc gagacggaa cagtaccgc 
      gaaatgcac ccgttaccc tcgactgcg aatttagtg 
      tccaagcat gaggacgat cttatcggg ggttcgtcc 
      taccgaatt ttcggttcg tctaacact ttacgcaag 
      cgatcgaac tcggttgtg agtcttttg cgcaaaatt 
      gtgcgaagt gatagcact tcaaacgag tcggagcag 
      gagatgtgg gagaaaata ccgcggact aagctcgag 
      gaagttatc gaagaggtg gacgagcaa ctgacaatt 
      atgtcttcg cgtgagaac gatttgaag cggccccga 
      attcaggat attttcgga ccgacgacg caacccctt 
      gatagtccc caggaggaa tctccgtcg ttcgattcg 
      ttgaaacag aagcgtgaa gaagagaga ttgcggagg 
      tttaggcgt aatctggcc aactatctc gagcacaag 
      gagccatcg tgacacttt tgatacttg aataaataa 
      gttaaatcg at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY