Status
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Gene information
General information
TC007872 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2001827..2006591
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007872
    iBeetle screen iB_04391

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    Pupal morphology
    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 7

    oblique dorsal thoracic muscles median partiallynot present - Number of animals showing the phenotype: 7

    oblique dorsal thoracic muscles median partiallyfragmented - Number of animals showing the phenotype: 7
    abdomen circumference increased - Number of animals showing the phenotype: 7

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    ovipositor elongated - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007872 RA
      MPKWAGKKC RLLSMLGLT TSFFFVEIV VGYITNSMA 
      LVADSFHML SDVAALMVA FVSVKMSPK KWSKNTFGW 
      ARAEVLGAL VNAVFLVAL CFSITVEAC KRFIESEAI 
      HDPQLLVIV GALGLFVNC IGLLLFHEH GSSHGHSHG 
      RLAHSRNRL TQLAMTDDN ENDESYPSP PPEKSSHHG 
      HSSPGQMNM RGVFLHVLS DALGSVIVI ISAVVFWLS 
      SWKYRNYID PALSILLVL LIMNSVWPL LRDSALILL 
      QTVPTHIQV DAIQRRLLA KVDGVVGVH EFHVWQLAG 
      DRIIASAHI KCRNLSEYM KIAEKVKEF FHNEGIHST 
      TIQPEFIDY QAPEITDTD QTEDCVLDC PKTTDSDDC 
      VQNTCCGPS KQGRETPSP NNTPYMVRQ RGNSSNSSN 
      NSQEMNEME AGVLLEKDK NNLNTTE
    • >TC007872 RB
      MPKWAGKKC RLLSMLGLT TSFFFVEIV VGYITNSMA 
      LVADSFHML SDVAALMVA FVSVKMSPK KWSKNTFGW 
      ARAEVLGAL VNAVFLVAL CFSITVEAC KRFIESEAI 
      HDPQLLVIV GALGLFVNC IGLLLFHEH GSSHGHSHG 
      RLAHSRNRL TQLAMTDDN ENDESYPSP PPEKSSHHG 
      HSSPGQMNM RGVFLHVLS DALGSVIVI ISAVVFWLS 
      SWKYRNYID PALSILLVL LIMNSVWPL LRDSALILL 
      QTVPTHIQV DAIQRRLLA KVDGVVGVH EFHVWQLAG 
      DRIIASAHI KCRNLSEYM KIAEKVKEF FHNEGIHST 
      TIQPEFIDY QAPEITDTD QTEDCVLDC PKTTDSDDC 
      VQNTCCGPS KQGRETPSP NNTPYMVRQ RGNSSNSSN 
      NSQEMNEME AGVLLEKGN SPVPKDYNC ISRGSQCSQ 
      YSVRPGSEP SF
    CDS sequence
    • >TC007872 RA
      atgccaaaa tgggccgga aaaaagtgt cgtctgctc 
      tcgatgttg gggctgacg acgtccttc ttcttcgtc 
      gaaatagtc gtaggttac ataacaaat tcgatggct 
      ttggtcgcg gacagtttc cacatgcta agcgacgtg 
      gccgccttg atggtggcc tttgtctca gtcaagatg 
      tctccgaag aagtggtcg aaaaacacg ttcggttgg 
      gccagagct gaagtcctt ggtgcttta gtcaacgcc 
      gtcttcctc gtagctctt tgtttttcc attacagtg 
      gaagcttgc aaacggttc atcgagagc gaagcgatt 
      cacgaccca caattgttg gtcattgtg ggtgcgttg 
      ggtctgttt gttaattgt ataggactg ctgttgttt 
      catgagcat ggtagtagc catggtcat tcccatggt 
      cgtttggcc catagtcga aaccgccta acacaacta 
      gccatgact gacgataac gaaaacgac gaaagttat 
      cccagtcct ccgccggag aaatcctcc catcatggg 
      cactcaagc cccggacag atgaacatg aggggggtt 
      ttcctccac gtgttgtcc gacgcttta ggctcagtt 
      atcgtaatt atatcagca gttgtcttc tggctgtcg 
      tcgtggaaa tacaggaat tacatcgac ccagctttg 
      agcatactg ctggtattg ttaataatg aactctgta 
      tggcctttg ttgcgagac tcggcttta attttacta 
      caaactgtc ccgacgcat attcaagtc gacgcaatc 
      cagcggagg ttactggcg aaagtcgac ggagtcgta 
      ggagtgcac gagtttcac gtctggcaa ttggccgga 
      gaccgaatc atagcttca gcccacatt aagtgccgt 
      aatttatcc gaatacatg aaaatcgcg gaaaaagtc 
      aaagaattc ttccacaac gaaggcatc cactcgacg 
      acaatccaa ccagagttc attgattac caagctccg 
      gaaatcaca gatactgac caaaccgaa gattgtgtt 
      cttgattgt cccaaaacc acagacagt gacgactgc 
      gtgcaaaac acatgctgc ggtccatcg aaacaagga 
      cgtgagacg ccctcgccc aataacacc ccgtatatg 
      gtccgtcag cgcggcaat tccagcaac agttcgaac 
      aattcgcag gaaatgaac gaaatggag gcgggagtg 
      cttttggaa aaagataag aacaatctc aatacgacg 
      gagtga
    • >TC007872 RB
      atgccaaaa tgggccgga aaaaagtgt cgtctgctc 
      tcgatgttg gggctgacg acgtccttc ttcttcgtc 
      gaaatagtc gtaggttac ataacaaat tcgatggct 
      ttggtcgcg gacagtttc cacatgcta agcgacgtg 
      gccgccttg atggtggcc tttgtctca gtcaagatg 
      tctccgaag aagtggtcg aaaaacacg ttcggttgg 
      gccagagct gaagtcctt ggtgcttta gtcaacgcc 
      gtcttcctc gtagctctt tgtttttcc attacagtg 
      gaagcttgc aaacggttc atcgagagc gaagcgatt 
      cacgaccca caattgttg gtcattgtg ggtgcgttg 
      ggtctgttt gttaattgt ataggactg ctgttgttt 
      catgagcat ggtagtagc catggtcat tcccatggt 
      cgtttggcc catagtcga aaccgccta acacaacta 
      gccatgact gacgataac gaaaacgac gaaagttat 
      cccagtcct ccgccggag aaatcctcc catcatggg 
      cactcaagc cccggacag atgaacatg aggggggtt 
      ttcctccac gtgttgtcc gacgcttta ggctcagtt 
      atcgtaatt atatcagca gttgtcttc tggctgtcg 
      tcgtggaaa tacaggaat tacatcgac ccagctttg 
      agcatactg ctggtattg ttaataatg aactctgta 
      tggcctttg ttgcgagac tcggcttta attttacta 
      caaactgtc ccgacgcat attcaagtc gacgcaatc 
      cagcggagg ttactggcg aaagtcgac ggagtcgta 
      ggagtgcac gagtttcac gtctggcaa ttggccgga 
      gaccgaatc atagcttca gcccacatt aagtgccgt 
      aatttatcc gaatacatg aaaatcgcg gaaaaagtc 
      aaagaattc ttccacaac gaaggcatc cactcgacg 
      acaatccaa ccagagttc attgattac caagctccg 
      gaaatcaca gatactgac caaaccgaa gattgtgtt 
      cttgattgt cccaaaacc acagacagt gacgactgc 
      gtgcaaaac acatgctgc ggtccatcg aaacaagga 
      cgtgagacg ccctcgccc aataacacc ccgtatatg 
      gtccgtcag cgcggcaat tccagcaac agttcgaac 
      aattcgcag gaaatgaac gaaatggag gcgggagtg 
      cttttggaa aaaggtaat tctccagtg cccaaagac 
      tacaattgt atctcaagg ggttcacag tgttcgcaa 
      tactcagta agacctggt tcagaacca agtttctga
    mRNA sequence
    • >TC007872 RA
      aggacaaaa aaattaccg ttttgtgaa ttttttttg 
      tttatttgt tgcttattt atgtttgtg ttgacgatt 
      tttctcttg acgtcatta gaaactcgt ccgttcatt 
      ggctgaaat gttacagtg gtctattgt ctgtcagtt 
      cttcagttg tggtgattc tatggtagt gaagtgtcc 
      ttccttctg tcaaattgt atgacgtac gcttatgta 
      ttaaataat taacaagaa gctcagctt ttccattaa 
      ccactacag tgttttcag ttaggcgtc taattaatg 
      tcttatttc gttttaaca aaaaccctc caagtttag 
      gcaaatatg ctgtgatat cgtgtttac aagtgctta 
      cacaggaga ctgtaaaaa tctacaaaa actgaccca 
      tgcttctta tttcagtaa tatggtgac acaagtgag 
      acagtttta acaaaaaac agatcacaa tgccaaaat 
      gggccggaa aaaagtgtc gtctgctct cgatgttgg 
      ggctgacga cgtccttct tcttcgtcg aaatagtcg 
      taggttaca taacaaatt cgatggctt tggtcgcgg 
      acagtttcc acatgctaa gcgacgtgg ccgccttga 
      tggtggcct ttgtctcag tcaagatgt ctccgaaga 
      agtggtcga aaaacacgt tcggttggg ccagagctg 
      aagtccttg gtgctttag tcaacgccg tcttcctcg 
      tagctcttt gtttttcca ttacagtgg aagcttgca 
      aacggttca tcgagagcg aagcgattc acgacccac 
      aattgttgg tcattgtgg gtgcgttgg gtctgtttg 
      ttaattgta taggactgc tgttgtttc atgagcatg 
      gtagtagcc atggtcatt cccatggtc gtttggccc 
      atagtcgaa accgcctaa cacaactag ccatgactg 
      acgataacg aaaacgacg aaagttatc ccagtcctc 
      cgccggaga aatcctccc atcatgggc actcaagcc 
      ccggacaga tgaacatga ggggggttt tcctccacg 
      tgttgtccg acgctttag gctcagtta tcgtaatta 
      tatcagcag ttgtcttct ggctgtcgt cgtggaaat 
      acaggaatt acatcgacc cagctttga gcatactgc 
      tggtattgt taataatga actctgtat ggcctttgt 
      tgcgagact cggctttaa ttttactac aaactgtcc 
      cgacgcata ttcaagtcg acgcaatcc agcggaggt 
      tactggcga aagtcgacg gagtcgtag gagtgcacg 
      agtttcacg tctggcaat tggccggag accgaatca 
      tagcttcag cccacatta agtgccgta atttatccg 
      aatacatga aaatcgcgg aaaaagtca aagaattct 
      tccacaacg aaggcatcc actcgacga caatccaac 
      cagagttca ttgattacc aagctccgg aaatcacag 
      atactgacc aaaccgaag attgtgttc ttgattgtc 
      ccaaaacca cagacagtg acgactgcg tgcaaaaca 
      catgctgcg gtccatcga aacaaggac gtgagacgc 
      cctcgccca ataacaccc cgtatatgg tccgtcagc 
      gcggcaatt ccagcaaca gttcgaaca attcgcagg 
      aaatgaacg aaatggagg cgggagtgc ttttggaaa 
      aagataaga acaatctca atacgacgg agtgaaaga 
      gcaagattt caaaaaatg atcgcgtac ttacattgt 
      atcatgtga tgaataagg gcgacagaa taaaatcaa 
      gtattaatt gggcggtag ctaaacgtc aaaaccagt 
      accttaaaa aggcccttc atcgtttac acgtaacta 
      gattaggaa attttctcg gctattttg caaacttgt 
      atacctacc gtgttgggt gaagtacaa gtgttacat 
      atttatatt ttattttgt atattaagt attatgtta 
      catactttt tattcaaca tctaaaaac aaggttata 
      ctatattta aaatcacag acactgtgt gcagcctat 
      tgatgaaat aaaattaaa ggaagacta aaaagtgag 
      ctttatttg gtgggcttt accttacct aatactcat 
      cttcggacc tcttttccc agtcaaaca atatagctt 
      aggagacga a
    • >TC007872 RB
      aggacaaaa aaattaccg ttttgtgaa ttttttttg 
      tttatttgt tgcttattt atgtttgtg ttgacgatt 
      tttctcttg acgtcatta gaaactcgt ccgttcatt 
      ggctgaaat gttacagtg gtctattgt ctgtcagtt 
      cttcagttg tggtgattc tatggtagt gaagtgtcc 
      ttccttctg tcaaattgt atgacgtac gcttatgta 
      ttaaataat taacaagaa gctcagctt ttccattaa 
      ccactacag tgttttcag ttaggcgtc taattaatg 
      tcttatttc gttttaaca aaaaccctc caagtttag 
      gcaaatatg ctgtgatat cgtgtttac aagtgctta 
      cacaggaga ctgtaaaaa tctacaaaa actgaccca 
      tgcttctta tttcagtaa tatggtgac acaagtgag 
      acagtttta acaaaaaac agatcacaa tgccaaaat 
      gggccggaa aaaagtgtc gtctgctct cgatgttgg 
      ggctgacga cgtccttct tcttcgtcg aaatagtcg 
      taggttaca taacaaatt cgatggctt tggtcgcgg 
      acagtttcc acatgctaa gcgacgtgg ccgccttga 
      tggtggcct ttgtctcag tcaagatgt ctccgaaga 
      agtggtcga aaaacacgt tcggttggg ccagagctg 
      aagtccttg gtgctttag tcaacgccg tcttcctcg 
      tagctcttt gtttttcca ttacagtgg aagcttgca 
      aacggttca tcgagagcg aagcgattc acgacccac 
      aattgttgg tcattgtgg gtgcgttgg gtctgtttg 
      ttaattgta taggactgc tgttgtttc atgagcatg 
      gtagtagcc atggtcatt cccatggtc gtttggccc 
      atagtcgaa accgcctaa cacaactag ccatgactg 
      acgataacg aaaacgacg aaagttatc ccagtcctc 
      cgccggaga aatcctccc atcatgggc actcaagcc 
      ccggacaga tgaacatga ggggggttt tcctccacg 
      tgttgtccg acgctttag gctcagtta tcgtaatta 
      tatcagcag ttgtcttct ggctgtcgt cgtggaaat 
      acaggaatt acatcgacc cagctttga gcatactgc 
      tggtattgt taataatga actctgtat ggcctttgt 
      tgcgagact cggctttaa ttttactac aaactgtcc 
      cgacgcata ttcaagtcg acgcaatcc agcggaggt 
      tactggcga aagtcgacg gagtcgtag gagtgcacg 
      agtttcacg tctggcaat tggccggag accgaatca 
      tagcttcag cccacatta agtgccgta atttatccg 
      aatacatga aaatcgcgg aaaaagtca aagaattct 
      tccacaacg aaggcatcc actcgacga caatccaac 
      cagagttca ttgattacc aagctccgg aaatcacag 
      atactgacc aaaccgaag attgtgttc ttgattgtc 
      ccaaaacca cagacagtg acgactgcg tgcaaaaca 
      catgctgcg gtccatcga aacaaggac gtgagacgc 
      cctcgccca ataacaccc cgtatatgg tccgtcagc 
      gcggcaatt ccagcaaca gttcgaaca attcgcagg 
      aaatgaacg aaatggagg cgggagtgc ttttggaaa 
      aaggtaatt ctccagtgc ccaaagact acaattgta 
      tctcaaggg gttcacagt gttcgcaat actcagtaa 
      gacctggtt cagaaccaa gtttctgat accttgatg 
      tttcacata ttttaatta ggctctagt taccttatt 
      aagtataaa aagtcgtgt tttcgtgtt aataacatc 
      acttcagtt ttgtaatat gctctacca gtaagtttc 
      acagatttt gttactact acagtgtga atctagtct 
      tagtacaat tttgttttc agttttatt acttgtagt 
      tcggctatg gccaagttg gtaaagcat atttttcta 
      gataagaac aatctcaat acgacggag tgaaagagc 
      aagatttca aaaaatgat cgcgtactt acattgtat 
      catgtgatg aataagggc gacagaata aaatcaagt 
      attaattgg gcggtagct aaacgtcaa aaccagtac 
      cttaaaaag gcccttcat cgtttacac gtaactaga 
      ttaggaaat tttctcggc tattttgca aacttgtat 
      acctaccgt gttgggtga agtacaagt gttacatat 
      ttatatttt attttgtat attaagtat tatgttaca 
      tacttttta ttcaacatc taaaaacaa ggttatact 
      atatttaaa atcacagac actgtgtgc agcctattg 
      atgaaataa aattaaagg aagactaaa aagtgagct 
      ttatttggt gggctttac cttacctaa tactcatct 
      tcggacctc ttttcccag tcaaacaat atagcttag 
      gagacgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY