Status
Please wait ...
Gene information
General information
TC007855 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1594213..1595706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007855
    iBeetle screen iB_04389

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007855 RA
      MSTDEDEED VKPEDAGEG PEPYPYGKY EGGRDDQLD 
RHGFGRAVL PNGDIYEGH YSHGKRHGR GLYVLKNGA 
RYDGEWKKG LKYGTGMFI YPDGSKYVG EWKRDLKQG 
KGTYYYVNG DTYEGSWYK GFRHGYGTY IYKSLNVIH 
TGNWKDGRM EGPGIIDYG CYKYHGKFE KNLPKGPGC 
FVFDNNKFM QHGFYVNLR NPAFDYVGA DKLALEDTN 
LESPEYRGN PRGIVPLWR ARSVSLYNA ELLPPEPLD 
LPIVDSQES LIDIIGYLQ RFDQDGVGG KAEEEEQHL 
PSPVTEKGD ATEEMPIPI N
    CDS sequence
    • >TC007855 RA
      atgtcaacg gatgaagac gaagaagat gtaaaacct 
gaagacgcc ggggagggg cctgaacct tacccttat 
ggaaaatat gagggtggg agggacgat caattggac 
cgacacggc tttggaagg gctgttttg ccaaatggg 
gatatttac gaggggcac tactcgcac gggaaacgg 
cacggcagg gggctgtat gtgctcaag aatggggcc 
agatatgac ggcgagtgg aaaaagggg ctaaaatac 
gggactggg atgtttatt taccccgac ggtagcaag 
tacgtcggt gagtggaaa cgcgacttg aaacagggc 
aaaggaacc tattattat gttaacggg gatacttat 
gagggcagt tggtacaaa ggatttcgg cacggctac 
ggcacctac atatataaa tcactcaac gtaattcac 
accgggaat tggaaggac ggccgaatg gaagggcca 
ggaataatc gattacggg tgctacaaa taccacgga 
aagttcgaa aaaaacctc ccgaaaggc cccggttgt 
ttcgttttc gataacaat aagttcatg cagcatgga 
ttttacgtg aatttgaga aacccggca tttgattac 
gttggcgct gataaactc gctcttgaa gatacgaat 
ttagaaagt ccagaatat cggggtaat cccagggga 
attgttccg ttgtggaga gcaagatct gtgagtctg 
tacaacgcc gaacttcta ccaccggaa cctctcgac 
ttgccaatc gttgactcg caagagagt cttatcgat 
attatcggt tatttgcag cggtttgac caagacggt 
gtgggaggg aaggccgaa gaagaggag caacacttg 
ccgtcaccc gtaaccgaa aaaggagac gccacggaa 
gaaatgcca atccccata aattga
    mRNA sequence
    • >TC007855 RA
      cggcaaata tttgttgtg taatgaaaa ttaatttta 
acgttatgt tattataaa tgatgcacc ggacttggc 
acaaaaata ataaatcgg cttaattaa ttccaatct 
agagatcgt tgtgtcagc gtcattaat aaagctgaa 
aaaatgtca acggatgaa gacgaagaa gatgtaaaa 
cctgaagac gccggggag gggcctgaa ccttaccct 
tatggaaaa tatgagggt gggagggac gatcaattg 
gaccgacac ggctttgga agggctgtt ttgccaaat 
ggggatatt tacgagggg cactactcg cacgggaaa 
cggcacggc agggggctg tatgtgctc aagaatggg 
gccagatat gacggcgag tggaaaaag gggctaaaa 
tacgggact gggatgttt atttacccc gacggtagc 
aagtacgtc ggtgagtgg aaacgcgac ttgaaacag 
ggcaaagga acctattat tatgttaac ggggatact 
tatgagggc agttggtac aaaggattt cggcacggc 
tacggcacc tacatatat aaatcactc aacgtaatt 
cacaccggg aattggaag gacggccga atggaaggg 
ccaggaata atcgattac gggtgctac aaataccac 
ggaaagttc gaaaaaaac ctcccgaaa ggccccggt 
tgtttcgtt ttcgataac aataagttc atgcagcat 
ggattttac gtgaatttg agaaacccg gcatttgat 
tacgttggc gctgataaa ctcgctctt gaagatacg 
aatttagaa agtccagaa tatcggggt aatcccagg 
ggaattgtt ccgttgtgg agagcaaga tctgtgagt 
ctgtacaac gccgaactt ctaccaccg gaacctctc 
gacttgcca atcgttgac tcgcaagag agtcttatc 
gatattatc ggttatttg cagcggttt gaccaagac 
ggtgtggga gggaaggcc gaagaagag gagcaacac 
ttgccgtca cccgtaacc gaaaaagga gacgccacg 
gaagaaatg ccaatcccc ataaattga ctcaataaa 
ttcgcttta ttata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY