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Gene information
General information
TC007825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1071821..1076385
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007825
    iBeetle screen iB_04385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007825 RA
      MSCFNCLSD RKLDSDLID KALERGQRL RSALVQSIL 
      THDPFNLDV VEPPRLDDN KIRAFLQGD PLSRQEEVW 
      ALETLRSMS ATDLCKVTD RTYRSEEPC DCLTCHEYR 
      EKDLDCDLK RENSNEITQ KIQERYMNE FASYVKFVD 
      SVKVSVNSV LLNEAGDKK VLNGLVKDK ASSVGQTFF 
      LEYQIPEIM KNNKAKSKI DFGNVVRLC ARRSQQVIN 
      FKQTSVHDV ANFNRVKLE DAIITFRLS FRSFKQKQP 
      TMLGVAHFN FSSFQVSKN FTCNQELTL RLHGNAPIV 
      IGGLKISVQ LGCGRLYFG KEFIDAVTN NNKENSVAL 
      GSDDLSSPE CNIPETMLD LYSPKTTQK TFEAFVPKL 
      LPKSNRKPS SVNRQPDSG KPITRDPKS AKKSDKKTE 
      SVPEIVPER PVLFGFIYV SEALFNREV MNSYLTCHM 
      FALDEVSSS KLVAFDSNP IYNFSQQIP LILEEDFLL 
      KLRENFMVV EFFEKGQGK DFLIGLAKL PLHQFYLAY 
      RNSVIVKYL SERKLPVIG ADGWESVFS PSNGEVVGQ 
      AQILLALGT KEQIENLES ERGFRKNTV KAKTPVRKK 
      INVATQSEM EPQKDNEPV VPTQKTNPV VETSTNTEP 
      EMEVRSTSD LLDTLQKAL SIDNVREKN YFRAHIAIN 
      SALHLPSRR KCKSKKSKG RNGKHEDIL PSTYVTFEC 
      FKGELKMTQ IVPKNTSPK WDFSCDVSL PLEYLTDNQ 
      KFLVFKVWR KSTNTTMTP NMQVDHVIG FATVDLTVL 
      AAGLPSVQG WFNINDLSK KCNGQINIH VTPLENVIK 
      YRPQECCEG ESSSPSQPP EAPFEPGEL LGRALKRKF 
      TELDEITQR LRLRLSKVT NDESDTSND EIADEFERD 
      INTLCVEDD FDMVDFEKE AQKFNLNHT KDSEVGLLQ 
      TDENLVENK QKIDHLLEK LSLISGEMS SRYVSGCSY 
      DAEAILNEL DSGSRPNLH SGVSFNREM SERFGGFDS 
      ERSGPEGEG QSNIVKSDQ HLI
    CDS sequence
    • >TC007825 RA
      atgtcgtgt tttaattgc ctttcggac cgaaaactc 
      gactcagat ttgatagat aaggcccta gagcgtggc 
      cagagactt cgaagtgcc ttggtgcag tcaattctg 
      acccatgac ccctttaac ctggacgtg gtagagccc 
      ccaaggctt gatgataac aaaattcgg gcctttctg 
      cagggggac cccttgtct cgccaggag gaggtctgg 
      gctcttgaa acattgcgg tcaatgtca gccactgat 
      ctttgcaaa gtcacagac aggacctac cgaagtgag 
      gaaccgtgt gactgtctc acttgccac gaatatcgg 
      gagaaagac ctggattgt gacttaaaa cgggaaaat 
      tcaaacgaa atcacccaa aaaattcaa gaacgatac 
      atgaatgag tttgccagt tatgtcaag tttgttgat 
      tcggttaaa gttagtgtg aatagtgtc ttgctgaat 
      gaagccggg gacaaaaaa gtgttaaat gggttggtt 
      aaagacaag gcgtcaagt gtgggccag acttttttt 
      cttgaatat cagattccc gaaattatg aaaaataat 
      aaggcgaag tcgaaaatt gactttggg aatgtagta 
      aggctttgt gcacgaagg tcgcaacaa gttattaat 
      tttaagcaa acttcggtc cacgacgtg gccaatttt 
      aatcgtgtc aagttggaa gatgcgata attacgttc 
      cggttaagt tttcgtagt tttaagcaa aaacaaccg 
      actatgtta ggagttgcg cattttaat tttagttcg 
      tttcaagtc tctaaaaac tttacttgt aatcaggaa 
      ttaactcta cgattgcac gggaatgcc cctattgtt 
      atcgggggt ttgaaaata tcggttcag ttgggctgt 
      ggccgactt tatttcggc aaagaattc atagacgca 
      gtcactaat aacaataaa gagaattca gtggcttta 
      gggagcgac gatttgagc agtcccgag tgtaatatt 
      cccgagaca atgttagat ttgtattct ccaaaaacc 
      acacaaaaa acattcgag gcttttgtg ccgaaattg 
      ttgcctaaa agtaaccga aagccgagt tcggttaac 
      cgacaaccc gattcaggg aaaccgatt actcgggac 
      ccaaaatca gcgaaaaaa tctgacaaa aaaacagaa 
      tcggttcct gaaatagtg ccagaaagg ccggttttg 
      ttcggtttt atttacgtt tctgaggcc ctttttaac 
      cgagaagtg atgaattcg tacttgact tgtcatatg 
      tttgctttg gacgaggtt tctagcagc aaattggtg 
      gcttttgat tcgaatcca atttataat ttttcccag 
      caaattccg ctgattctt gaggaggat tttttgctc 
      aagttgcgt gagaatttc atggtcgtg gaatttttt 
      gaaaaaggg caagggaag gatttttta atcggactt 
      gcgaaactt cctctacac cagttttat ctggcttac 
      aggaattcg gtcattgtg aagtattta tcggaaaga 
      aaacttcct gtaattgga gctgacggt tgggagtcg 
      gtgtttagc cccagcaat ggtgaagtg gtaggtcaa 
      gcgcaaatc ctcctcgct ctaggtacg aaagaacag 
      atcgaaaac ctcgagagt gagcgagga tttaggaaa 
      aacacagtc aaggcaaaa actcccgtt aggaaaaaa 
      attaatgta gcaacacaa tccgaaatg gagccccaa 
      aaagacaat gaaccggtg gttcccact caaaaaacg 
      aatccagta gtcgaaacg agcacaaac accgaaccg 
      gaaatggaa gtccgaagt acttccgac ctactcgat 
      acgttacaa aaggcctta tccatcgat aacgtcaga 
      gaaaaaaat tatttccga gcccacatt gcaataaac 
      agcgcttta catttgccc agtcgtcga aaatgcaaa 
      tcgaaaaaa tccaaagga cgaaacggc aaacacgag 
      gatatccta ccgagtact tacgtcaca tttgagtgt 
      tttaagggc gaattgaaa atgactcaa atcgtaccc 
      aaaaatacc tcacccaag tgggacttt agctgcgat 
      gtttcttta ccgctggag tatttaaca gacaatcag 
      aaatttttg gtgttcaaa gtttggcgc aaatcgacc 
      aatacgaca atgacaccg aacatgcaa gtcgatcac 
      gtgatcggt ttcgcgacc gttgatttg accgtgttg 
      gctgccgga ttgccaagt gtgcaaggg tggttcaat 
      atcaacgat ttgtcgaaa aaatgcaac ggacaaatt 
      aatattcac gtgactccg ttggaaaat gtgataaaa 
      tatcgtccg caagaatgc tgcgagggt gagtcttcg 
      agtccttca cagcccccc gaagccccc ttcgagcct 
      ggtgagctt ctggggcgc gccctcaag cgaaaattc 
      accgaattg gacgaaatc acacagaga ttaagactg 
      cgcctttca aaagtcaca aacgacgaa tcagatact 
      tcgaatgat gaaatcgcc gatgaattt gaacgcgat 
      attaacaca ctttgtgtg gaggatgat ttcgacatg 
      gtcgatttt gagaaggaa gcccagaaa tttaactta 
      aaccacacg aaagattct gaagtcgga ttattacaa 
      actgatgag aatttggtc gaaaacaag caaaaaatc 
      gaccattta ttggagaaa ttgagcttg atttctggc 
      gaaatgtcc agtagatat gtatcaggg tgttcctat 
      gacgcagaa gcgatttta aacgagtta gatagtggt 
      tcaaggccg aatttgcac tcgggggtt tcgttcaat 
      cgggagatg tctgaaagg tttggaggt tttgacagt 
      gagaggtca ggaccggag ggagaggga caaagcaat 
      attgttaaa agtgatcaa catttaata taa
    mRNA sequence
    • >TC007825 RA
      ccaaacaaa caaaaattg tgttctgcc agttgtcga 
      ttaattcga taaaaagta ttgattgaa agtgcagtg 
      gtgtttttt gataaagaa taatgagcg attcggaaa 
      cagagaatt tgttagaag aaccctgga atattggaa 
      tgatcggca ataattccg gaaaaaaca aaattacga 
      tatgaaaaa ttcttaaat gaagcacct gatgaagaa 
      ttttgatta ataagctca ctgtgatat taagagtct 
      cagggactt gtagaagag aggaattat ataacatga 
      ctattataa taataggag taatataca acggcctct 
      gagttctgt gactaatgc aactgattc agttctaat 
      tgtgcgcaa acgagtcgt attactatt aacaactgt 
      agcaattgt acttttcac ttttagtat gaataaata 
      attagtaaa ttttgaatt tttatatta cttttactt 
      accgttcca tgaaaaatt taatttaat ttgttatgt 
      tactcagtg tacaataaa aatgttaac aaaatctgt 
      gttttatta cccccaaac aactgtttt tgtaagttt 
      cattgcaaa ttttgttta aaataaata taaaacgtt 
      ttcattttg tttaaaatg aaaatatat ttttttatt 
      ttaaacaat ttgcaatga aacttgtaa gataaaaat 
      aataaaaaa caatataaa aggaattta ttcaaacgt 
      ggcgcttta ccgtggtag taacgcact tcagcacca 
      gaggcgctg gattctcgt gttgccagt gatttacta 
      gggaattat cacttctta acccttact gttctgtgt 
      agggagtaa gagggggtg tttgtgtgt ggttttttg 
      gcccaaaaa acgccgaat tatcattag tgagtatct 
      agagcctcg taatcacaa ttagcgtaa aaaccgtac 
      ggttgagtt aacatttaa tcaaaaaga cgccaccaa 
      attgctgcc attgattga tctagtgac ctgacttgg 
      gaacatttc gttgtgctt gccctcctg tcaattgac 
      agttttcca aaatacaaa catatcaac cgttttaat 
      ttcatattt tttggaaat gtcgtgttt taattgcct 
      ttcggaccg aaaactcga ctcagattt gatagataa 
      ggccctaga gcgtggcca gagacttcg aagtgcctt 
      ggtgcagtc aattctgac ccatgaccc ctttaacct 
      ggacgtggt agagccccc aaggcttga tgataacaa 
      aattcgggc ctttctgca gggggaccc cttgtctcg 
      ccaggagga ggtctgggc tcttgaaac attgcggtc 
      aatgtcagc cactgatct ttgcaaagt cacagacag 
      gacctaccg aagtgagga accgtgtga ctgtctcac 
      ttgccacga atatcggga gaaagacct ggattgtga 
      cttaaaacg ggaaaattc aaacgaaat cacccaaaa 
      aattcaaga acgatacat gaatgagtt tgccagtta 
      tgtcaagtt tgttgattc ggttaaagt tagtgtgaa 
      tagtgtctt gctgaatga agccgggga caaaaaagt 
      gttaaatgg gttggttaa agacaaggc gtcaagtgt 
      gggccagac tttttttct tgaatatca gattcccga 
      aattatgaa aaataataa ggcgaagtc gaaaattga 
      ctttgggaa tgtagtaag gctttgtgc acgaaggtc 
      gcaacaagt tattaattt taagcaaac ttcggtcca 
      cgacgtggc caattttaa tcgtgtcaa gttggaaga 
      tgcgataat tacgttccg gttaagttt tcgtagttt 
      taagcaaaa acaaccgac tatgttagg agttgcgca 
      ttttaattt tagttcgtt tcaagtctc taaaaactt 
      tacttgtaa tcaggaatt aactctacg attgcacgg 
      gaatgcccc tattgttat cgggggttt gaaaatatc 
      ggttcagtt gggctgtgg ccgacttta tttcggcaa 
      agaattcat agacgcagt cactaataa caataaaga 
      gaattcagt ggctttagg gagcgacga tttgagcag 
      tcccgagtg taatattcc cgagacaat gttagattt 
      gtattctcc aaaaaccac acaaaaaac attcgaggc 
      ttttgtgcc gaaattgtt gcctaaaag taaccgaaa 
      gccgagttc ggttaaccg acaacccga ttcagggaa 
      accgattac tcgggaccc aaaatcagc gaaaaaatc 
      tgacaaaaa aacagaatc ggttcctga aatagtgcc 
      agaaaggcc ggttttgtt cggttttat ttacgtttc 
      tgaggccct ttttaaccg agaagtgat gaattcgta 
      cttgacttg tcatatgtt tgctttgga cgaggtttc 
      tagcagcaa attggtggc ttttgattc gaatccaat 
      ttataattt ttcccagca aattccgct gattcttga 
      ggaggattt tttgctcaa gttgcgtga gaatttcat 
      ggtcgtgga attttttga aaaagggca agggaagga 
      ttttttaat cggacttgc gaaacttcc tctacacca 
      gttttatct ggcttacag gaattcggt cattgtgaa 
      gtatttatc ggaaagaaa acttcctgt aattggagc 
      tgacggttg ggagtcggt gtttagccc cagcaatgg 
      tgaagtggt aggtcaagc gcaaatcct cctcgctct 
      aggtacgaa agaacagat cgaaaacct cgagagtga 
      gcgaggatt taggaaaaa cacagtcaa ggcaaaaac 
      tcccgttag gaaaaaaat taatgtagc aacacaatc 
      cgaaatgga gccccaaaa agacaatga accggtggt 
      tcccactca aaaaacgaa tccagtagt cgaaacgag 
      cacaaacac cgaaccgga aatggaagt ccgaagtac 
      ttccgacct actcgatac gttacaaaa ggccttatc 
      catcgataa cgtcagaga aaaaaatta tttccgagc 
      ccacattgc aataaacag cgctttaca tttgcccag 
      tcgtcgaaa atgcaaatc gaaaaaatc caaaggacg 
      aaacggcaa acacgagga tatcctacc gagtactta 
      cgtcacatt tgagtgttt taagggcga attgaaaat 
      gactcaaat cgtacccaa aaatacctc acccaagtg 
      ggactttag ctgcgatgt ttctttacc gctggagta 
      tttaacaga caatcagaa atttttggt gttcaaagt 
      ttggcgcaa atcgaccaa tacgacaat gacaccgaa 
      catgcaagt cgatcacgt gatcggttt cgcgaccgt 
      tgatttgac cgtgttggc tgccggatt gccaagtgt 
      gcaagggtg gttcaatat caacgattt gtcgaaaaa 
      atgcaacgg acaaattaa tattcacgt gactccgtt 
      ggaaaatgt gataaaata tcgtccgca agaatgctg 
      cgagggtga gtcttcgag tccttcaca gccccccga 
      agccccctt cgagcctgg tgagcttct ggggcgcgc 
      cctcaagcg aaaattcac cgaattgga cgaaatcac 
      acagagatt aagactgcg cctttcaaa agtcacaaa 
      cgacgaatc agatacttc gaatgatga aatcgccga 
      tgaatttga acgcgatat taacacact ttgtgtgga 
      ggatgattt cgacatggt cgattttga gaaggaagc 
      ccagaaatt taacttaaa ccacacgaa agattctga 
      agtcggatt attacaaac tgatgagaa tttggtcga 
      aaacaagca aaaaatcga ccatttatt ggagaaatt 
      gagcttgat ttctggcga aatgtccag tagatatgt 
      atcagggtg ttcctatga cgcagaagc gattttaaa 
      cgagttaga tagtggttc aaggccgaa tttgcactc 
      gggggtttc gttcaatcg ggagatgtc tgaaaggtt 
      tggaggttt tgacagtga gaggtcagg accggaggg 
      agagggaca aagcaatat tgttaaaag tgatcaaca 
      tttaatata aaggtctaa ataattttc caagcgtaa 
      ttttttgtt gattttttg tggtggaag taaacgtat 
      cgcagcaga cgtgccttc gtggttgtg ggcaaatga 
      acagttgcg gattttctg aaagtagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY