Status
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Gene information
General information
TC007814 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 949898..958545
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007814
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04383

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007814 RA
      MTEKRPSSV SYKSEQPSD DENAQLSLD PQTQLYLAH 
      LSQQNGCWG RFFPIPFER AAQKSWWDP TFDSEILEE 
      QYRRSSNPH SRFKFRYAL WYMLLISSS WLVYFLVTG 
      LVSETHHWP MLCTVLGTL ILSVTTVLF LTYTDIYNN 
      HMLLMSLIV AFVLCVISL SFVVLVPMK TDPLSFPIT 
      SDISPVGHF SICIEILML IYTLIPLKL YMSITIGFL 
      YSAAFETLT ISLQNQSLE ASTITVRLL SHFCIHVIG 
      LHILIMTNV RMRNTFMKV GQSLLVRRQ LESEKKLKE 
      NMIHSLMPS SVAEWLLSE EETFTRSDQ RNSDPETND 
      IRSLFRPFN MNRMENVSI LFADIVGFT KMASKKSAE 
      ELVEILNNL FQRFDHLCK FHNCEKIST LGDCYYCVS 
      GCPEPRHDH AKCCVEMGL SMIQAIKQF DQEKNEGVN 
      MRVGVHTGT VLCGIVGTR RFKFDVWSN DVTLANRME 
      STGKPGMVH VSEKTCEFL QDMYLLEEG EPVLGMKTY 
      FILGRTGDK TPSYSGSFR AEGRQKYTN SLQLFVSPP 
      TSSPSMSPQ SRPRVLSCD TTHIKATHN PNMLSPDVC 
      KIKASSLPS ILDSENEQD GGDICKETS DCDNIKTPT 
      STASSGKYS VKLKNWKMP KFLRKNDGK PELDIEVPA 
      NSEDRISGG YQQVPTIIE TSNVSNGKK KDSFISVTD 
      VDDDNKRSS IFLDDPKDT IDVKSYISQ SRSDIGQYD 
      YSPGDLNQF MRTGSYRSQ YGRSPNEFS FLQRAGSNR 
      SRRGRSPNF DVFPGERQR STTVCVGNL ERPKMSLEV 
      PGRLTNISV LDDHFSVYS RKDSGIRSN SRRSSIQQL 
      EQATHPNSE HRVSGYYTS SQSTVTSPQ YNVRMPGVF 
      KDKFGTCIQ NLRKQSDRQ LIKCVQENS KSRGSYFIK 
      PPLRRLTLF FKDKQMEQD YRTNVHSLF EKADSILTL 
      ADSKFNTYF DVCVSSLVF AIVSISLWL LYGHTTLWL 
      VMSGVFVAI QACGWALCS RKVVECSDR CLRCVSRFY 
      RWNMFGAVL VSLPLVTVL INFCTKVFT TSETSNYLY 
      TYLLFVGVI HFCNFTQLN CWMKNCLAV AFTLIFIPL 
      FLNGTCSNF FVEKSERTE LNPIVAILD DRNRTEVKS 
      LSLEHYSFL YKSEILMDL FLLLVLVWL LNREFEIGY 
      RLSFHANYV SNRDKINVQ NLKTQADYL IYNIVPEHV 
      AEQLKKNAK YSENFKDVG IIFASIVNF NEMYDESYL 
      GGKEYLRVL NELIADFDE LLDRPEFAS VEKIKTIGS 
      TFMAASGLN STTRRNQKD PNEHLYALM DFALEMQNV 
      ITDFNRDLL EFNLILRIG YNFGEVTAA VIGNTKLYY 
      DIWGDAVNI ASRMDSTGV NGCIQIGEN CLQVLETRY 
      VFRPRGSVY VKGKDNMSV YLLERKKTD DEIEA
    CDS sequence
    • >TC007814 RA
      atgacggag aagagaccc tcgtcggtg agttacaag 
      tcggagcag ccctccgat gacgagaac gcccagctt 
      tcgctggat ccgcagacg cagctctac ctcgcgcac 
      ttgtcgcag cagaacggc tgctgggga cgctttttt 
      cccattccc ttcgaacgg gcagcgcaa aagtcatgg 
      tgggacccc acgttcgac tcggaaatt ctagaggag 
      cagtacagg aggagctct aacccgcat agtaggttc 
      aaatttagg tacgcacta tggtacatg ttgctgatt 
      tcgtcatcg tggttggtg tattttctg gtcacgggg 
      ttggtatca gagacacac cattggccc atgttgtgt 
      actgtctta ggaacattg atactgtca gtgacgaca 
      gtacttttc ctcacttat accgacatt tacaacaac 
      cacatgctc ctcatgtca ctaatcgtg gcgtttgtt 
      ttatgtgtg ataagtctt tcgttcgtc gtattggta 
      ccaatgaag acagacccg cttagtttc ccaataact 
      tccgatata agtccagtc ggtcacttc tcaatatgc 
      attgaaatt ctgatgttg atttacacg ttaattcct 
      ttgaaactg tacatgagc atcacgatt ggtttttta 
      tactctgcc gcgtttgaa acactaacg atttcactt 
      caaaaccaa tcgcttgaa gcttcaacg atcacagtc 
      cggttatta tcacacttt tgtattcac gtaatcggc 
      ctccacatc cttattatg acaaacgtc cgtatgcga 
      aacactttc atgaaagtg gggcaaagt cttctcgtc 
      cgccgccaa ttggagtcg gagaaaaag ttaaaagaa 
      aacatgatt cactccttg atgccttcc agcgtcgcc 
      gaatggctc ctctccgaa gaggagaca ttcacacga 
      agcgatcag cgcaattcc gaccccgaa accaacgac 
      attcgatca ctttttcgc ccgtttaac atgaaccga 
      atggaaaac gtaagcatt ctctttgcc gatattgtc 
      ggttttact aaaatggcc agcaagaaa agtgccgaa 
      gaactcgtg gaaatccta aataattta tttcaaaga 
      ttcgaccat ttgtgcaaa ttccacaat tgcgagaaa 
      atatcaacg ttgggtgat tgttactat tgtgtttct 
      ggttgtccg gaaccgaga catgatcat gccaaatgt 
      tgcgtcgaa atggggctt agtatgata caagctatt 
      aaacaattc gatcaagag aaaaacgaa ggtgtgaat 
      atgagagtt ggggtgcat acggggaca gttctttgt 
      ggtattgtg gggacacga aggtttaaa tttgatgtt 
      tggagtaat gatgttacg cttgccaat cggatggaa 
      tcgactggg aaaccagga atggtgcac gtgtcggag 
      aaaacatgc gagttccta caggatatg tacttgctt 
      gaggaaggg gagccggtt ttagggatg aaaacgtat 
      tttattttg ggtcggacc ggagacaaa acgccttcc 
      tacagtggt agttttcgt gctgaaggc cgtcaaaag 
      tacacaaat tcgttgcaa ttattcgtt tcaccgcca 
      acttcgtcg ccttccatg tcgcctcaa tcccgacct 
      agagtcctt tcttgcgat acaactcac ataaaagca 
      acgcacaat ccgaatatg ttatcgcct gatgtttgt 
      aaaatcaaa gcgagtagt ctcccaagt atccttgat 
      tcggaaaac gaacaagac ggcggcgat atttgtaaa 
      gaaacatcc gactgtgat aatattaaa acgccaact 
      tcgacagcc agctccggc aaatacagt gtcaagctg 
      aaaaactgg aaaatgccg aaattcctt cgaaaaaac 
      gacggaaaa cccgaattg gatattgaa gttccggcc 
      aattcggaa gaccgaata tcgggcggc taccaacaa 
      gttccgacc atcattgaa acttcgaat gtttcgaat 
      gggaaaaag aaagattcg tttattagc gtaacggac 
      gtcgatgat gataataaa agaagtagt atttttcta 
      gacgatccg aaagataca attgatgtc aaatcgtac 
      atcagccaa tcgcggagc gatatcggc caatatgat 
      tattcccca ggtgacttg aatcaattt atgaggact 
      ggaagttac cgttcacaa tacggcaga tctccgaat 
      gaattttca ttcctacag cgtgccgga agtaaccga 
      tctcgacgt ggtcgttcc ccgaatttc gatgttttt 
      cccggggaa cgacagaga agtacaacg gtgtgtgtt 
      ggtaatctc gaacggccg aaaatgtcg cttgaagtc 
      cccggaaga ctcacaaat atctccgtc cttgacgat 
      catttctcg gtctacagt cgcaaagat tcgggaatt 
      aggagcaac agtcgcagg agtagcatt cagcagttg 
      gagcaagca acgcacccg aattcggaa caccgtgtt 
      tctggctat tatacctcg agtcagagt acggtgact 
      tcgccccaa tacaacgtc cgaatgcct ggtgtgttc 
      aaggacaag tttggtaca tgtattcaa aacttgcgt 
      aaacaatca gatcgtcaa ttaatcaag tgcgtacaa 
      gaaaactcc aagtcgcga ggttcctat tttatcaag 
      ccgccgttg cgtcggctg acgttattc ttcaaggac 
      aaacaaatg gagcaggat taccgaaca aatgtccat 
      tcgttgttt gaaaaagcc gactcgatt ctaacactt 
      gccgattcg aaattcaat acgtacttt gatgtgtgt 
      gtttcgagt ctcgttttt gcaattgtt tcgatctcg 
      ttgtggctt ttgtacgga catacgacc ctatggttg 
      gtcatgtcg ggtgttttc gttgcgatt caagcgtgt 
      ggttgggcg ctgtgttcg cgaaaagtc gtcgaatgt 
      tcggatcgg tgtctgcgg tgcgtttcg cgattttac 
      aggtggaat atgttcggt gctgttttg gtaagtttg 
      ccgctcgtt accgttttg ataaatttt tgtacgaaa 
      gtattcaca acgtcggaa acgtccaac tatttatac 
      acttatctg ctttttgtc ggagtcata catttctgt 
      aattttaca caactgaat tgctggatg aagaattgt 
      ctcgctgtg gcgttcact cttattttt attccgttg 
      tttttgaac ggcacctgt tcgaacttt ttcgtcgaa 
      aagtccgaa cggaccgaa ctcaatccg atcgttgcg 
      attctagac gatcgcaat cggaccgaa gttaagtcg 
      ttaagtctc gaacattac agttttctg tacaaatcg 
      gagatttta atggacttg ttcctgctg ttggtgctg 
      gtttggtta cttaaccgg gagtttgag atcggttac 
      cggttgagt ttccacgcc aactacgtc tcaaaccgg 
      gacaaaatc aacgtacag aatctcaaa acgcaagcc 
      gattatctc atttataac atcgtacct gaacacgtg 
      gccgaacag ttgaaaaag aacgcaaag tattcggag 
      aatttcaaa gacgtgggg attattttc gcgagtatt 
      gttaacttt aacgaaatg tatgacgag agctacttg 
      ggtgggaag gaatatttg agggttttg aacgaactg 
      attgccgat tttgacgaa ttgctcgat cgtcccgaa 
      tttgcaagt gtggagaaa attaaaact atcggtagc 
      acttttatg gcagctagt ggtctcaac tcgaccacg 
      cgtcgtaac cagaaagac cctaatgag catttgtac 
      gcgcttatg gattttgcg ttagaaatg caaaacgtt 
      ataaccgat ttcaaccgc gatttgctc gaatttaat 
      ctgatcctt cggatcggt tataacttt ggcgaggtg 
      acagctgcg gttatcggt aatacgaag ttgtattac 
      gatatatgg ggtgatgcg gttaatatt gcctcacga 
      atggactcg actggggtc aatggttgt attcaaatc 
      ggggagaat tgtctgcaa gttctcgaa acgcggtac 
      gtttttcgg ccgcggggt tcggtctat gtcaagggt 
      aaagataac atgagtgtg tatctgttg gagcggaag 
      aagaccgat gatgagatt gaagcttaa
    mRNA sequence
    • >TC007814 RA
      attatgaaa attagcagc gaatccgcg aaaagcgca 
      agccaaaca tcggtgttg acacacagc ggtaatgtt 
      tgattttga gtgtaaaca tgacgaggg tgttctcca 
      acatatgca atgaattat tgccgagat aatgtgtta 
      gacgtttcg caatgacgg agaagagac cctcgtcgg 
      tgagttaca agtcggagc agccctccg atgacgaga 
      acgcccagc tttcgctgg atccgcaga cgcagctct 
      acctcgcgc acttgtcgc agcagaacg gctgctggg 
      gacgctttt ttcccattc ccttcgaac gggcagcgc 
      aaaagtcat ggtgggacc ccacgttcg actcggaaa 
      ttctagagg agcagtaca ggaggagct ctaacccgc 
      atagtaggt tcaaattta ggtacgcac tatggtaca 
      tgttgctga tttcgtcat cgtggttgg tgtattttc 
      tggtcacgg ggttggtat cagagacac accattggc 
      ccatgttgt gtactgtct taggaacat tgatactgt 
      cagtgacga cagtacttt tcctcactt ataccgaca 
      tttacaaca accacatgc tcctcatgt cactaatcg 
      tggcgtttg ttttatgtg tgataagtc tttcgttcg 
      tcgtattgg taccaatga agacagacc cgcttagtt 
      tcccaataa cttccgata taagtccag tcggtcact 
      tctcaatat gcattgaaa ttctgatgt tgatttaca 
      cgttaattc ctttgaaac tgtacatga gcatcacga 
      ttggttttt tatactctg ccgcgtttg aaacactaa 
      cgatttcac ttcaaaacc aatcgcttg aagcttcaa 
      cgatcacag tccggttat tatcacact tttgtattc 
      acgtaatcg gcctccaca tccttatta tgacaaacg 
      tccgtatgc gaaacactt tcatgaaag tggggcaaa 
      gtcttctcg tccgccgcc aattggagt cggagaaaa 
      agttaaaag aaaacatga ttcactcct tgatgcctt 
      ccagcgtcg ccgaatggc tcctctccg aagaggaga 
      cattcacac gaagcgatc agcgcaatt ccgaccccg 
      aaaccaacg acattcgat cactttttc gcccgttta 
      acatgaacc gaatggaaa acgtaagca ttctctttg 
      ccgatattg tcggtttta ctaaaatgg ccagcaaga 
      aaagtgccg aagaactcg tggaaatcc taaataatt 
      tatttcaaa gattcgacc atttgtgca aattccaca 
      attgcgaga aaatatcaa cgttgggtg attgttact 
      attgtgttt ctggttgtc cggaaccga gacatgatc 
      atgccaaat gttgcgtcg aaatggggc ttagtatga 
      tacaagcta ttaaacaat tcgatcaag agaaaaacg 
      aaggtgtga atatgagag ttggggtgc atacgggga 
      cagttcttt gtggtattg tggggacac gaaggttta 
      aatttgatg tttggagta atgatgtta cgcttgcca 
      atcggatgg aatcgactg ggaaaccag gaatggtgc 
      acgtgtcgg agaaaacat gcgagttcc tacaggata 
      tgtacttgc ttgaggaag gggagccgg ttttaggga 
      tgaaaacgt attttattt tgggtcgga ccggagaca 
      aaacgcctt cctacagtg gtagttttc gtgctgaag 
      gccgtcaaa agtacacaa attcgttgc aattattcg 
      tttcaccgc caacttcgt cgccttcca tgtcgcctc 
      aatcccgac ctagagtcc tttcttgcg atacaactc 
      acataaaag caacgcaca atccgaata tgttatcgc 
      ctgatgttt gtaaaatca aagcgagta gtctcccaa 
      gtatccttg attcggaaa acgaacaag acggcggcg 
      atatttgta aagaaacat ccgactgtg ataatatta 
      aaacgccaa cttcgacag ccagctccg gcaaataca 
      gtgtcaagc tgaaaaact ggaaaatgc cgaaattcc 
      ttcgaaaaa acgacggaa aacccgaat tggatattg 
      aagttccgg ccaattcgg aagaccgaa tatcgggcg 
      gctaccaac aagttccga ccatcattg aaacttcga 
      atgtttcga atgggaaaa agaaagatt cgtttatta 
      gcgtaacgg acgtcgatg atgataata aaagaagta 
      gtatttttc tagacgatc cgaaagata caattgatg 
      tcaaatcgt acatcagcc aatcgcgga gcgatatcg 
      gccaatatg attattccc caggtgact tgaatcaat 
      ttatgagga ctggaagtt accgttcac aatacggca 
      gatctccga atgaatttt cattcctac agcgtgccg 
      gaagtaacc gatctcgac gtggtcgtt ccccgaatt 
      tcgatgttt ttcccgggg aacgacaga gaagtacaa 
      cggtgtgtg ttggtaatc tcgaacggc cgaaaatgt 
      cgcttgaag tccccggaa gactcacaa atatctccg 
      tccttgacg atcatttct cggtctaca gtcgcaaag 
      attcgggaa ttaggagca acagtcgca ggagtagca 
      ttcagcagt tggagcaag caacgcacc cgaattcgg 
      aacaccgtg tttctggct attatacct cgagtcaga 
      gtacggtga cttcgcccc aatacaacg tccgaatgc 
      ctggtgtgt tcaaggaca agtttggta catgtattc 
      aaaacttgc gtaaacaat cagatcgtc aattaatca 
      agtgcgtac aagaaaact ccaagtcgc gaggttcct 
      attttatca agccgccgt tgcgtcggc tgacgttat 
      tcttcaagg acaaacaaa tggagcagg attaccgaa 
      caaatgtcc attcgttgt ttgaaaaag ccgactcga 
      ttctaacac ttgccgatt cgaaattca atacgtact 
      ttgatgtgt gtgtttcga gtctcgttt ttgcaattg 
      tttcgatct cgttgtggc ttttgtacg gacatacga 
      ccctatggt tggtcatgt cgggtgttt tcgttgcga 
      ttcaagcgt gtggttggg cgctgtgtt cgcgaaaag 
      tcgtcgaat gttcggatc ggtgtctgc ggtgcgttt 
      cgcgatttt acaggtgga atatgttcg gtgctgttt 
      tggtaagtt tgccgctcg ttaccgttt tgataaatt 
      tttgtacga aagtattca caacgtcgg aaacgtcca 
      actatttat acacttatc tgctttttg tcggagtca 
      tacatttct gtaatttta cacaactga attgctgga 
      tgaagaatt gtctcgctg tggcgttca ctcttattt 
      ttattccgt tgtttttga acggcacct gttcgaact 
      ttttcgtcg aaaagtccg aacggaccg aactcaatc 
      cgatcgttg cgattctag acgatcgca atcggaccg 
      aagttaagt cgttaagtc tcgaacatt acagttttc 
      tgtacaaat cggagattt taatggact tgttcctgc 
      tgttggtgc tggtttggt tacttaacc gggagtttg 
      agatcggtt accggttga gtttccacg ccaactacg 
      tctcaaacc gggacaaaa tcaacgtac agaatctca 
      aaacgcaag ccgattatc tcatttata acatcgtac 
      ctgaacacg tggccgaac agttgaaaa agaacgcaa 
      agtattcgg agaatttca aagacgtgg ggattattt 
      tcgcgagta ttgttaact ttaacgaaa tgtatgacg 
      agagctact tgggtggga aggaatatt tgagggttt 
      tgaacgaac tgattgccg attttgacg aattgctcg 
      atcgtcccg aatttgcaa gtgtggaga aaattaaaa 
      ctatcggta gcactttta tggcagcta gtggtctca 
      actcgacca cgcgtcgta accagaaag accctaatg 
      agcatttgt acgcgctta tggattttg cgttagaaa 
      tgcaaaacg ttataaccg atttcaacc gcgatttgc 
      tcgaattta atctgatcc ttcggatcg gttataact 
      ttggcgagg tgacagctg cggttatcg gtaatacga 
      agttgtatt acgatatat ggggtgatg cggttaata 
      ttgcctcac gaatggact cgactgggg tcaatggtt 
      gtattcaaa tcggggaga attgtctgc aagttctcg 
      aaacgcggt acgtttttc ggccgcggg gttcggtct 
      atgtcaagg gtaaagata acatgagtg tgtatctgt 
      tggagcgga agaagaccg atgatgaga ttgaagctt 
      aattatttt atttgtgtg cctgcaaaa ttgcattcc 
      atagctgct tttagctaa gagtgacca agtgtagcc 
      ttaaagcca gaaataggt gtttgtcgg ccgattttt 
      agtcttacc aaagagtag tcgtgtaag gaaaatgtc 
      aattttgtt ggatggttg cgttaccaa agaggcgat 
      ttttggaga gattttcga agtaactgt gataatcaa 
      cttctcact tagtgattt tagcgtagt taaaggccc 
      taattgatt tttatttta tttgacgca agaggacaa 
      acacctagt tgtatttct tgccaataa gtacaaacc 
      gatttgtaa aattccgta aggtgtgcg gacgtattt 
      tatactcat tattttata cagtaattt tatattgta 
      ataccaaag agtgggatt ttattatcg cttggcaaa 
      tttgatgtt agactgatg tcggcattc gtctttacc 
      tcagaaatt tgaccaact aaggaccat gtaaaaatt 
      attgtgatt agttgtttt tgtagtgtt ttcgactgg 
      tttgcgatt ttattgttt tgttgtttg tggataaaa 
      tcgtcgact cgacgaaaa caaacccgt gtaacgtgg 
      aaattgtat atgatatta tgcggtagc tttaaaata 
      actagctta tttgttagt ggagttcct aacatttgt 
      tttatttaa ttttttaaa tattcgtat ggaccgcat 
      gcggtggtt acgtattcc gttatattt aacgtacct 
      aattgtttt aattgaatg ttgtattta tttattgag 
      ttgtgtaaa tagtcaaag ttttatttc acttgcatc 
      cccataagt gccttctat catttcagt tattttaac 
      gatatttcg ttcaaattc gtaacaaat tctactaga 
      tatgtttgt gcaccgtcc tatcatgta taatactga 
      tattatctc aataaaaca tgtaattaa tt
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