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Gene information
General information
TC007794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 628963..631290
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007794
    iBeetle screen iB_04382

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007794 RA
      MSFDRECPE MVHVAVNTD PLQMLLQDE FTKTHYGLK 
      VRNIPHTKI SEMNELKVW YDFGEKIGE GGFGIVMAV 
      REKSNNKQW AMKLISKST AGDRIKMVE QEIQILKLI 
      HHPHIIYLD KIFESPKKM YLVFERCHA DFAKIYNER 
      KPFAEKIAK KIISELVDA VAYLHKYEI VHRDLKLEN 
      ILLGENPLD PTDEYYIKL TDFGLSSIV KSGKGIESM 
      LHEFCGTIY YMAPEMITF RSYSQMCDM WSIGVILYM 
      LVIGKFPFY GKTEKEIQV KICTQSPKF DAKLSQEVE 
      DLILLLLLV KDPVKRIRA TEVLQHPWI LSKKMSRTM 
      HYNIMDKMK EWKSEMTVE TGAVSDWVM APDDVIEIN 
      RSQTKQAMN LPKTNINFD RASRRMSNP KKPSLKEQV 
      TRAKPKGQS GKKRVN
    • >TC007794 RB
      MSFDRECPE MVHVAVNTD PLQMLLQDE FTKTQHYGL 
      KVRNIPHTK ISEMNELKV WYDFGEKIG EGGFGIVMA 
      VREKSNNKQ WAMKLISKS TAGDRIKMV EQEIQILKL 
      IHHPHIIYL DKIFESPKK MYLVFERCH ADFAKIYNE 
      RKPFAEKIA KKIISELVD AVAYLHKYE IVHRDLKLE 
      NILLGENPL DPTDEYYIK LTDFGLSSI VKSGKGIES 
      MLHEFCGTI YYMAPEMIT FRSYSQMCD MWSIGVILY 
      MLVIGKFPF YGKTEKEIQ VKICTQSPK FDAKLSQEV 
      EDLILLLLL VKDPVKRIR ATEVLQHPW ILSKKMSRT 
      MHYNIMDKM KEWKSEMTV ETGAVSDWV MAPDDVIEI 
      NRSQTKQAM NLPKTNINF DRASRRMSN PKKPSLKEQ 
      VTRAKPKGQ SGKKRVN
    CDS sequence
    • >TC007794 RA
      atgtcattt gaccgtgaa tgtcctgaa atggttcac 
      gttgcagtc aatacggac cctctccaa atgttgtta 
      caagacgaa ttcacaaaa acgcactat ggcctaaaa 
      gtacgcaat atcccacac acaaaaatc tccgaaatg 
      aacgaactg aaagtttgg tacgatttt ggggaaaaa 
      atcggcgaa ggcggattt ggcatcgta atggcagtg 
      cgcgaaaaa agcaacaat aaacaatgg gcgatgaaa 
      ctcatctcc aaatcaaca gccggtgat cggataaaa 
      atggtggag caggaaata caaatcttg aaattaatc 
      caccaccct catataatc taccttgac aagattttc 
      gaatctccg aaaaaaatg tacctggtg tttgagcgt 
      tgtcacgcc gatttcgca aaaatctac aacgagcgc 
      aagcccttt gctgaaaaa atcgccaag aagataatt 
      tcggaactg gtcgatgct gtggcgtat ttgcacaaa 
      tacgaaatc gttcataga gacttgaag ttggaaaat 
      attctactc ggggaaaac ccgctcgac ccaaccgac 
      gaatactac atcaaacta accgacttt ggcttgagc 
      agcatcgtc aagtcgggc aaaggcatc gaaagcatg 
      ttgcacgaa ttttgcgga acgatttat tacatggcg 
      cctgaaatg attaccttc aggtcgtac agtcaaatg 
      tgtgacatg tggtcaatt ggtgtgatt ctctacatg 
      ctagtgatt gggaaattt ccattttat ggcaaaacc 
      gaaaaagaa atccaagtg aaaatttgc acacaatcg 
      cctaaattt gatgcgaaa ttgtcgcaa gaagtcgaa 
      gatttgatt ttgttgttg ttgttggtc aaagatcca 
      gtcaaacgc atacgggcg acggaggtt ttgcaacat 
      ccctggatt ttgtccaag aaaatgtcc aggacgatg 
      cattacaat attatggat aagatgaag gagtggaag 
      tcggaaatg acggtggaa acaggtgcg gtgtctgat 
      tgggtaatg gctccagac gacgtaatt gaaatcaac 
      cggtcacaa acaaagcaa gcgatgaat ctaccaaaa 
      actaatatt aattttgac agggctagt aggaggatg 
      agtaacccg aagaaacct agtctaaag gaacaagta 
      acaagagcg aaaccgaaa ggacagtct ggcaaaaag 
      cgggtgaat taa
    • >TC007794 RB
      atgtcattt gaccgtgaa tgtcctgaa atggttcac 
      gttgcagtc aatacggac cctctccaa atgttgtta 
      caagacgaa ttcacaaaa acgcagcac tatggccta 
      aaagtacgc aatatccca cacacaaaa atctccgaa 
      atgaacgaa ctgaaagtt tggtacgat tttggggaa 
      aaaatcggc gaaggcgga tttggcatc gtaatggca 
      gtgcgcgaa aaaagcaac aataaacaa tgggcgatg 
      aaactcatc tccaaatca acagccggt gatcggata 
      aaaatggtg gagcaggaa atacaaatc ttgaaatta 
      atccaccac cctcatata atctacctt gacaagatt 
      ttcgaatct ccgaaaaaa atgtacctg gtgtttgag 
      cgttgtcac gccgatttc gcaaaaatc tacaacgag 
      cgcaagccc tttgctgaa aaaatcgcc aagaagata 
      atttcggaa ctggtcgat gctgtggcg tatttgcac 
      aaatacgaa atcgttcat agagacttg aagttggaa 
      aatattcta ctcggggaa aacccgctc gacccaacc 
      gacgaatac tacatcaaa ctaaccgac tttggcttg 
      agcagcatc gtcaagtcg ggcaaaggc atcgaaagc 
      atgttgcac gaattttgc ggaacgatt tattacatg 
      gcgcctgaa atgattacc ttcaggtcg tacagtcaa 
      atgtgtgac atgtggtca attggtgtg attctctac 
      atgctagtg attgggaaa tttccattt tatggcaaa 
      accgaaaaa gaaatccaa gtgaaaatt tgcacacaa 
      tcgcctaaa tttgatgcg aaattgtcg caagaagtc 
      gaagatttg attttgttg ttgttgttg gtcaaagat 
      ccagtcaaa cgcatacgg gcgacggag gttttgcaa 
      catccctgg attttgtcc aagaaaatg tccaggacg 
      atgcattac aatattatg gataagatg aaggagtgg 
      aagtcggaa atgacggtg gaaacaggt gcggtgtct 
      gattgggta atggctcca gacgacgta attgaaatc 
      aaccggtca caaacaaag caagcgatg aatctacca 
      aaaactaat attaatttt gacagggct agtaggagg 
      atgagtaac ccgaagaaa cctagtcta aaggaacaa 
      gtaacaaga gcgaaaccg aaaggacag tctggcaaa 
      aagcgggtg aattaa
    mRNA sequence
    • >TC007794 RA
      accaacttc aagcatgtc atttgaccg tgaatgtcc 
      tgaaatggt tcacgttgc agtcaatac ggaccctct 
      ccaaatgtt gttacaaga cgaattcac aaaaacgca 
      ctatggcct aaaagtacg caatatccc acacacaaa 
      aatctccga aatgaacga actgaaagt ttggtacga 
      ttttgggga aaaaatcgg cgaaggcgg atttggcat 
      cgtaatggc agtgcgcga aaaaagcaa caataaaca 
      atgggcgat gaaactcat ctccaaatc aacagccgg 
      tgatcggat aaaaatggt ggagcagga aatacaaat 
      cttgaaatt aatccacca ccctcatat aatctacct 
      tgacaagat tttcgaatc tccgaaaaa aatgtacct 
      ggtgtttga gcgttgtca cgccgattt cgcaaaaat 
      ctacaacga gcgcaagcc ctttgctga aaaaatcgc 
      caagaagat aatttcgga actggtcga tgctgtggc 
      gtatttgca caaatacga aatcgttca tagagactt 
      gaagttgga aaatattct actcgggga aaacccgct 
      cgacccaac cgacgaata ctacatcaa actaaccga 
      ctttggctt gagcagcat cgtcaagtc gggcaaagg 
      catcgaaag catgttgca cgaattttg cggaacgat 
      ttattacat ggcgcctga aatgattac cttcaggtc 
      gtacagtca aatgtgtga catgtggtc aattggtgt 
      gattctcta catgctagt gattgggaa atttccatt 
      ttatggcaa aaccgaaaa agaaatcca agtgaaaat 
      ttgcacaca atcgcctaa atttgatgc gaaattgtc 
      gcaagaagt cgaagattt gattttgtt gttgttgtt 
      ggtcaaaga tccagtcaa acgcatacg ggcgacgga 
      ggttttgca acatccctg gattttgtc caagaaaat 
      gtccaggac gatgcatta caatattat ggataagat 
      gaaggagtg gaagtcgga aatgacggt ggaaacagg 
      tgcggtgtc tgattgggt aatggctcc agacgacgt 
      aattgaaat caaccggtc acaaacaaa gcaagcgat 
      gaatctacc aaaaactaa tattaattt tgacagggc 
      tagtaggag gatgagtaa cccgaagaa acctagtct 
      aaaggaaca agtaacaag agcgaaacc gaaaggaca 
      gtctggcaa aaagcgggt gaattaaaa tagccgcaa 
      agtaacgac tagttattg tgtaataat gtttacttt 
      acata
    • >TC007794 RB
      accaacttc aagcatgtc atttgaccg tgaatgtcc 
      tgaaatggt tcacgttgc agtcaatac ggaccctct 
      ccaaatgtt gttacaaga cgaattcac aaaaacgca 
      gcactatgg cctaaaagt acgcaatat cccacacac 
      aaaaatctc cgaaatgaa cgaactgaa agtttggta 
      cgattttgg ggaaaaaat cggcgaagg cggatttgg 
      catcgtaat ggcagtgcg cgaaaaaag caacaataa 
      acaatgggc gatgaaact catctccaa atcaacagc 
      cggtgatcg gataaaaat ggtggagca ggaaataca 
      aatcttgaa attaatcca ccaccctca tataatcta 
      ccttgacaa gattttcga atctccgaa aaaaatgta 
      cctggtgtt tgagcgttg tcacgccga tttcgcaaa 
      aatctacaa cgagcgcaa gccctttgc tgaaaaaat 
      cgccaagaa gataatttc ggaactggt cgatgctgt 
      ggcgtattt gcacaaata cgaaatcgt tcatagaga 
      cttgaagtt ggaaaatat tctactcgg ggaaaaccc 
      gctcgaccc aaccgacga atactacat caaactaac 
      cgactttgg cttgagcag catcgtcaa gtcgggcaa 
      aggcatcga aagcatgtt gcacgaatt ttgcggaac 
      gatttatta catggcgcc tgaaatgat taccttcag 
      gtcgtacag tcaaatgtg tgacatgtg gtcaattgg 
      tgtgattct ctacatgct agtgattgg gaaatttcc 
      attttatgg caaaaccga aaaagaaat ccaagtgaa 
      aatttgcac acaatcgcc taaatttga tgcgaaatt 
      gtcgcaaga agtcgaaga tttgatttt gttgttgtt 
      gttggtcaa agatccagt caaacgcat acgggcgac 
      ggaggtttt gcaacatcc ctggatttt gtccaagaa 
      aatgtccag gacgatgca ttacaatat tatggataa 
      gatgaagga gtggaagtc ggaaatgac ggtggaaac 
      aggtgcggt gtctgattg ggtaatggc tccagacga 
      cgtaattga aatcaaccg gtcacaaac aaagcaagc 
      gatgaatct accaaaaac taatattaa ttttgacag 
      ggctagtag gaggatgag taacccgaa gaaacctag 
      tctaaagga acaagtaac aagagcgaa accgaaagg 
      acagtctgg caaaaagcg ggtgaatta aaatagccg 
      caaagtaac gactagtta ttgtgtaat aatgtttac 
      tttacata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY