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Gene information
General information
TC007785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 34470..43254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007785
    iBeetle screen iB_04381

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007785 RA
      MSRLADYFV VVGYDHEKE RSGISSGII LQRFPEKDW 
      PDTPFIDGI EWFCQPQGW ALSTERQEP KFFVSVLTD 
      IDANRYYCA CLCFNETVS ITPSKPVDE EEDTVESEA 
      PLVRPIPAI SHHSIMYAP KCLVLVSRL DYIETFRNC 
      LGIIYSVYV ESMPVPLEN LVGNILGCI QVPPPGGPQ 
      VRFSIGAGD RQALQPPLS PSLPVTHTS VNLLFHQLG 
      IRNVLVLFC AIMTEHKIL FHSKSYNRL TEACRALTA 
      LMYPFRYSH VYIPLLPAA LVEFLSTPT PFIMGVHSS 
      LKYEVAELM DVIIADLDG GSITIPDGI SLPLLPEPL 
      LSHTQENLS LILQPELSC ADHAFPPLA VRAPQPAML 
      DKEIRAVFM RTFAQLLQG YRSCLTLIR IHPKPVITF 
      HKAAFLGER NLKECEFTT RVLDCMFFT SFVAERGPP 
      WRPCDVWDE LYSSLGELM KAEGQDSRL ILVHIQELA 
      QQLYTNENP NPQSYSQKI LVPPEGAFA RIHQPVLPV 
      INGEQVQTI IDEGLSKNN LKSRLASLR PIQPRIVPS 
      GPHISNLNE NKNIVSNSA RRLEVLRNC INCIFENKI 
      SDARKTFPA VLRALQSKQ ARLALCNEL AQHVSGTKA 
      MLEHQQFDL VVRLMNCGL QDDSSMDEH GVAAALLPL 
      STIFCRKLC TGIIQFAYT CIQEHAVWQ NQQFWEAAF 
      YQDVQKDIK ALYTARCES VRGELLSSP TSPRESKDY 
      NWTNRRSIL NRVHEPTAL EIAAEQMRI WSSIDGEKQ 
      KELITCEEN TMYSQAIHY ANRMVYLLI PLDIGNKTS 
      KQELFDDER ASNSMANSV AESDSVDAE SGFEDQDPG 
      ETGMTVIRL VSRFIDKVC NEGGVTREH IRNLHQMIP 
      GVVHMHIET LEAVYRESK RLPPIQKPK ILTPNMLAG 
      EELIMEGLR VYLLPDGRE ESSPGLLGG PPLLPAEGA 
      IFLTNYRII FKGIPCDSF ACEQIVVRS FPVTSLTKE 
      KKVAVQYLA HLDQWLQEG LQVRSCTFQ LMKLAFDEE 
      VTAENVETL RKFIHKIRH PPDVFHHFA FTGQVVVSQ 
      TPLHKGKEK NATLRGFAK KTLLKTARK AGFKQKSSS 
      KRQKYVLPN STLYNSNKY MTSPGRMSL PNTDEYSHD 
      DDLSIDDFE TGPSSVPSA PTDAKTLER MVERSYVRD 
      WQRLGLCND NSSPTNAKP APDQFRLTT VNSMYMMCR 
      SYPALLVVP ANVTDESIR RFCRCYRQG RIPSTTWRH 
      PRTKALLLR GAGHHGKSV MGMLKSHPT PNASTETTS 
      SFEQERYLS ALVSATPIF SLRHGSAWG MSDSSLSVN 
      SLFLAADDP LGGATLTPE VARRSKPLK GMGTLAMFP 
      RSSGGKGPK NFGRWGSLK DRRQSSQAS LANVQQNRG 
      NIRHSTDAE SAIYGVHTL QRAALYILG EKAQMKGVK 
      VESAPKTDF IPVEYYDVR HTKTAFKKL MRACIPSSA 
      TDAPEQSFH RLIENSEWL QQIQNIMQL AGAVVDLLD 
      LQGSSVAIC LEDGWDTTA QVSSVAQLC LDPYYRTLE 
      GFRVLVEKE WIGFGHRFS HRSNLNANS QASGFAPTF 
      LQFLDVVHQ IQKQFPMAF EFSDFYLRF LAYHSVSCR 
      FRTFLFDCE LERVESGVA AVEDKRGSL TSHHKSVDT 
      ISDDENIYP GGRVAGTTS CGGTNLGQS VFDYVEKQH 
      ARTPLFFNF MYTPDFEHP VLRPQSHLP CLEIWQYYL 
      DENLHHGPE YDLEIIQLD TQQEEEAEA ADGISSKSN 
      RKIITLGYD CVSKCVPDA FSHLLEEIH RLETELGHL 
      PQKWKVLWD KLELPTTDS LTRHASFST ALVRSHGRL 
      VHKRSTLEI LMRGKMVGA AETSLVYSH PHRFEKYNY 
      TTPTYCDYC TNLLWGPVK TGMRCMDCG YSCHEKCIE 
      GVPKNCTKY KAVTDNNAS QTISGTGGD NGSVSSGSA 
      RQTSSQQYY DQFSSNVAE NRTHEGYLY KRGALLKGW 
      KQRWFVLDS IKHQLRYYD AMEDSHFKG YIDLAEVMS 
      VTSASPAPG PPKKTDDKS FFDLRTSRR TYNFCAADA 
      ATAQEWIEK LQACLQ
    CDS sequence
    • >TC007785 RA
      atgtcacgt ttagcagac tatttcgtt gttgtgggt 
      tatgatcac gagaaagaa cggagcggt attagtagc 
      ggtataatt ttacaaaga tttccggaa aaagactgg 
      ccagatacc ccgtttatt gacggaatc gaatggttt 
      tgccaaccc caaggatgg gctttatcc accgagcgg 
      caagaaccg aaatttttt gtctcggta cttacagat 
      atcgacgca aatcgctac tattgcgct tgtctttgt 
      ttcaatgaa actgtctca attaccccc agtaagcct 
      gtagatgag gaggaagac actgttgaa agcgaggcc 
      cccctggta cgccccatc cccgccata tcccatcac 
      agcattatg tacgcccct aaatgtcta gtattggtc 
      tcgagattg gattatatt gaaacgttt agaaattgc 
      ttaggaatt atttattca gtttatgtg gaaagcatg 
      ccggtaccg ttggagaat ttagttgga aatatcctc 
      ggttgtatt caagtgcct ccgccaggg ggcccccaa 
      gtgaggttc agtataggg gctggggac agacaagcg 
      ctacagccc ccattgagc ccctcactg ccagtgacg 
      cacacttcc gttaactta ctgttccat cagttggga 
      attcgcaat gttttagtt ttattttgt gccataatg 
      accgaacat aagattttg tttcactca aagagctat 
      aaccggtta actgaagca tgtcgcgcg ttaactgct 
      ctcatgtac ccttttcgg tactcacac gtctacata 
      cctctatta ccagctgct ttggtcgag tttttatca 
      actccaacg ccttttatc atgggggtg cattcgtcc 
      ttgaaatat gaagtcgcc gaattgatg gatgtgatt 
      attgccgat ctagatggc ggttcaatt acaattcct 
      gatggaatt tcccttccg ctacttcca gagccgctc 
      ctgagtcac acgcaggaa aatctgagt ctgattctt 
      caacctgaa ctcagttgt gctgatcat gcgttccct 
      ccgttagca gtgcgggct ccacagccc gctatgtta 
      gataaggaa atcagagct gtttttatg cgaactttt 
      gcccaactt ctccaaggc tatcgaagt tgtctcact 
      ttgattaga attcatcca aaaccagtt ataacattt 
      cacaaagca gcttttctc ggtgaacgc aatttgaaa 
      gaatgcgaa tttacaacg cgtgttttg gattgtatg 
      tttttcaca agttttgtg gcagaaaga gggccacca 
      tggcggccg tgtgacgtc tgggatgaa ctctatagt 
      agtttgggt gaactcatg aaggctgaa ggacaggat 
      tctagatta attttggtt catatacaa gaattggcg 
      caacagttg tacactaac gagaatcca aatccacaa 
      tcgtactcg caaaagatt ttagtgcct ccggaagga 
      gcatttgca agaatacat caaccggtt ttacctgta 
      atcaacgga gaacaagta caaacgatt attgatgaa 
      ggattgtcc aaaaataat cttaaatcg aggttggca 
      tcactgcgc cccatccag ccgcgaatt gtaccttct 
      ggtccgcat atttccaac ctcaacgag aacaaaaac 
      attgtcagt aattcagca cgtcgtctt gaagtactt 
      cgcaattgt atcaattgc atttttgag aataaaatt 
      tcggatgca cgtaaaact ttcccagca gtgctacga 
      gccctccaa tcgaaacaa gcccgttta gccttatgt 
      aatgaactt gcacaacac gttagcggt acgaaagca 
      atgctcgaa catcaacaa ttcgatctt gttgtgcgt 
      ttaatgaat tgtggtctt caagatgat tcgtcaatg 
      gacgagcat ggcgttgct gctgctctt ttaccactt 
      tcgacaatt ttttgtcgg aaactttgt acggggatt 
      atacagttt gcatatact tgtattcag gagcatgca 
      gtttggcaa aatcaacaa ttttgggaa gctgctttc 
      tatcaagat gtacagaaa gatattaag gctttgtat 
      acggcgagg tgtgaaagt gtgagaggg gagttgtta 
      tcgagtcca acttcgccg agagagagt aaggattac 
      aattggacg aatcgaagg agtattttg aatcgagtc 
      cacgaacca actgcgtta gaaatagca gctgagcaa 
      atgagaatc tggagttcc atagatggg gaaaaacag 
      aaagagtta attacgtgc gaggagaat acgatgtac 
      agtcaagca attcattat gcgaatcgt atggtttat 
      ttactaatt cctttggat attggaaat aagacaagt 
      aaacaagag ttgtttgat gatgagagg gcgagtaat 
      agtatggcg aatagtgtc gctgaaagt gacagtgta 
      gacgctgag tcaggtttc gaagatcaa gatccaggt 
      gagactgga atgactgta attcgtctc gtttctcgt 
      ttcatcgat aaagtgtgc aatgaaggt ggtgtgaca 
      cgggaacac atccgaaat cttcatcaa atgattcct 
      ggagtcgtc catatgcac attgagact cttgaagcg 
      gtctatcgt gaatcgaaa cgacttcca ccgattcaa 
      aaaccgaaa attctaaca ccaaatatg ctcgccggt 
      gaagaactg ataatggaa ggtttgcgc gtttatctt 
      cttcctgat ggtagagaa gaaagtagt ccgggatta 
      ctcggtggt ccaccgctt cttccagca gagggcgcc 
      atctttctc acaaactat cgtattatt ttcaaggga 
      attccctgt gattcgttt gcttgcgag caaatcgtt 
      gtgagatca tttccagtg acgtcgttg actaaagaa 
      aaaaaagtt gcagtgcaa tatttagca cacttggat 
      caatggttg caagaggga ctacaggtg cgttcgtgt 
      acatttcaa ttgatgaaa ttggcgttt gacgaagaa 
      gttaccgct gaaaatgtc gaaacattg cgaaagttt 
      attcataaa attcggcat ccgcccgat gtttttcac 
      cattttgcg tttacgggc caagttgtt gtaagtcag 
      acaccattg cataaaggc aaggagaaa aatgcaact 
      ttgagagga tttgcaaag aaaacttta ttaaaaacg 
      gctcgcaaa gcaggattt aagcagaaa tcgtcatca 
      aaacgtcaa aaatatgtt ttaccaaat tcgactctt 
      tataatagt aataaatat atgacgagt ccaggtcgg 
      atgagtttg ccaaatacc gatgagtat agtcatgat 
      gatgatttg tcaattgat gattttgaa acgggacca 
      tcatcagtg ccttcagca cccactgat gcaaagact 
      ttggaacga atggttgaa cgtagttat gtgagagat 
      tggcaacga ttgggttta tgtaacgat aattcctca 
      ccgacaaat gctaaacca gctccagat cagttccga 
      ttaactaca gttaactcc atgtacatg atgtgtcgc 
      agttatccg gcactgttg gtggtacct gcaaatgta 
      accgatgag agtattcgt cgcttttgt cgttgttac 
      cgccagggt agaattcct agcaccact tggagacac 
      cctcgaaca aaagctctc ttattacga ggcgctggt 
      caccatggt aaaagtgta atgggaatg ttgaaaagt 
      catcccaca ccaaatgcc tccactgaa accacatca 
      tccttcgaa caagaaaga tatctttcg gctctcgtt 
      tcagccact ccaatattc tctctacgt catggctca 
      gcttggggc atgtctgat agttctcta agtgtaaat 
      tcgttgttt ctagcagct gatgatccc ttaggtggc 
      gcaacgcta acacctgaa gtagcccga cgttcaaaa 
      cctctcaaa ggaatggga actttggca atgtttcct 
      cggtcgtcc ggtggtaaa ggaccaaaa aatttcggt 
      cgttggggt tctttgaaa gacagaaga cagagttca 
      caagcatct cttgccaac gttcagcaa aatcgcggc 
      aatatcagg cattcaaca gatgccgaa agtgcaatt 
      tatggtgtt catacgctg caaagagct gcattgtat 
      attttggga gagaaggca caaatgaaa ggtgtgaaa 
      gtggaatcc gctccaaag actgatttt attccagtg 
      gagtattac gatgtgaga catacaaaa acagcattt 
      aaaaaattg atgagggcg tgcattccg agttcggca 
      acggatgca cccgaacaa agctttcac agactaatt 
      gaaaattcc gaatggctg caacaaatt cagaatata 
      atgcagctg gctggtgcc gttgtcgat cttcttgac 
      cttcagggc tcctctgta gccatttgt ctggaagac 
      ggttgggat acaacagct caagtctct tccgttgct 
      caactctgt ctcgatcct tactatcgc acacttgaa 
      ggttttcgt gttctagtc gaaaaagaa tggatcggt 
      tttggacac cgtttcagc catcgtagt aatctcaac 
      gcgaattca caagcgtca ggtttcgca cctactttt 
      cttcaattt ttggatgtc gtccatcaa attcaaaaa 
      caatttccc atggctttc gaattcagc gatttttat 
      ctacgtttt ttggcttat cattcggtt tcgtgtcgt 
      tttcgtacg tttttattc gattgcgaa ttagagaga 
      gtcgaaagt ggggttgct gcagttgag gataagaga 
      ggttcatta acttcgcat cataaaagt gtggacacc 
      atctccgat gatgagaat atttatccg ggtggaagg 
      gtcgcaggg acgacgagt tgtggtggc acaaatttg 
      ggacaaagt gtgtttgat tatgtggag aagcaacat 
      gctaggacc ccgttgttt ttcaatttt atgtacaca 
      ccggatttt gaacaccct gttttgagg ccacagtca 
      catttacca tgtctcgaa atatggcaa tactatttg 
      gatgagaat ctccatcat ggacctgaa tatgatctt 
      gaaattata cagcttgat acacaacaa gaagaagag 
      gcggaagcc gctgatggc atttcgagt aaaagtaat 
      cgaaaaata ataacacta ggttatgat tgtgttagt 
      aaatgtgtg ccagacgca ttcagtcat ttattggag 
      gaaatacat cgactcgaa acagaactc ggacattta 
      ccacaaaaa tggaaagtc ctttgggat aaactcgaa 
      ttgccaact accgattct cttactaga catgcctcg 
      tttagtaca gccctagtt cgttctcat ggtcgtttg 
      gtacacaaa cgttcaacc cttgaaatt ttgatgagg 
      ggtaaaatg gtgggtgct gccgaaacg tcattggtg 
      tattcacat cctcatcgt ttcgaaaaa tataattac 
      accacgccc acatattgt gattattgt acaaatttg 
      ttatgggga ccagtgaag accggtatg aggtgtatg 
      gattgtggg tacagttgc cacgagaaa tgtatagaa 
      ggcgtgccg aaaaattgc acgaaatat aaagcagtc 
      actgacaat aacgcatca cagacaatt tcgggtaca 
      ggaggtgat aacgggtcg gttagttcc ggtagcgca 
      cgccaaacc tctagtcag caatattac gatcagttt 
      tcatcgaat gttgccgaa aatcggaca cacgaaggg 
      tatttgtat aaaagaggg gcgttgtta aaaggctgg 
      aagcagcgc tggtttgtt ttggattca attaaacat 
      cagctaagg tattacgat gcgatggaa gactcacac 
      tttaaggga tatatagac ttagctgaa gttatgtcg 
      gtgacgtca gctagtcca gctcctgga cctccaaaa 
      aagactgac gacaaatca ttttttgat ttgcgcact 
      agtcgacgc acttataat ttttgtgca gcagatgct 
      gcgacagca caagaatgg atcgagaag ctgcaagct 
      tgtctacaa taa
    mRNA sequence
    • >TC007785 RA
      aaagatttg tttagatga atattactt gggtgtggc 
      tgttaacat aataagctc tagaaatgt attagtttt 
      gtaaacact taaaccagt ttttatccg aattaaagt 
      ttctcaagg aggatttgt gacagacta tcccgaggc 
      ccccaggtt atacgactt gctcatcat tattttgaa 
      attgtgact aacgaaaat gttaatttt actcaaaaa 
      cgacatgaa ttgaaaaat gtcacgttt agcagacta 
      tttcgttgt tgtgggtta tgatcacga gaaagaacg 
      gagcggtat tagtagcgg tataatttt acaaagatt 
      tccggaaaa agactggcc agatacccc gtttattga 
      cggaatcga atggttttg ccaacccca aggatgggc 
      tttatccac cgagcggca agaaccgaa attttttgt 
      ctcggtact tacagatat cgacgcaaa tcgctacta 
      ttgcgcttg tctttgttt caatgaaac tgtctcaat 
      tacccccag taagcctgt agatgagga ggaagacac 
      tgttgaaag cgaggcccc cctggtacg ccccatccc 
      cgccatatc ccatcacag cattatgta cgcccctaa 
      atgtctagt attggtctc gagattgga ttatattga 
      aacgtttag aaattgctt aggaattat ttattcagt 
      ttatgtgga aagcatgcc ggtaccgtt ggagaattt 
      agttggaaa tatcctcgg ttgtattca agtgcctcc 
      gccaggggg cccccaagt gaggttcag tataggggc 
      tggggacag acaagcgct acagccccc attgagccc 
      ctcactgcc agtgacgca cacttccgt taacttact 
      gttccatca gttgggaat tcgcaatgt tttagtttt 
      attttgtgc cataatgac cgaacataa gattttgtt 
      tcactcaaa gagctataa ccggttaac tgaagcatg 
      tcgcgcgtt aactgctct catgtaccc ttttcggta 
      ctcacacgt ctacatacc tctattacc agctgcttt 
      ggtcgagtt tttatcaac tccaacgcc ttttatcat 
      gggggtgca ttcgtcctt gaaatatga agtcgccga 
      attgatgga tgtgattat tgccgatct agatggcgg 
      ttcaattac aattcctga tggaatttc ccttccgct 
      acttccaga gccgctcct gagtcacac gcaggaaaa 
      tctgagtct gattcttca acctgaact cagttgtgc 
      tgatcatgc gttccctcc gttagcagt gcgggctcc 
      acagcccgc tatgttaga taaggaaat cagagctgt 
      ttttatgcg aacttttgc ccaacttct ccaaggcta 
      tcgaagttg tctcacttt gattagaat tcatccaaa 
      accagttat aacatttca caaagcagc ttttctcgg 
      tgaacgcaa tttgaaaga atgcgaatt tacaacgcg 
      tgttttgga ttgtatgtt tttcacaag ttttgtggc 
      agaaagagg gccaccatg gcggccgtg tgacgtctg 
      ggatgaact ctatagtag tttgggtga actcatgaa 
      ggctgaagg acaggattc tagattaat tttggttca 
      tatacaaga attggcgca acagttgta cactaacga 
      gaatccaaa tccacaatc gtactcgca aaagatttt 
      agtgcctcc ggaaggagc atttgcaag aatacatca 
      accggtttt acctgtaat caacggaga acaagtaca 
      aacgattat tgatgaagg attgtccaa aaataatct 
      taaatcgag gttggcatc actgcgccc catccagcc 
      gcgaattgt accttctgg tccgcatat ttccaacct 
      caacgagaa caaaaacat tgtcagtaa ttcagcacg 
      tcgtcttga agtacttcg caattgtat caattgcat 
      ttttgagaa taaaatttc ggatgcacg taaaacttt 
      cccagcagt gctacgagc cctccaatc gaaacaagc 
      ccgtttagc cttatgtaa tgaacttgc acaacacgt 
      tagcggtac gaaagcaat gctcgaaca tcaacaatt 
      cgatcttgt tgtgcgttt aatgaattg tggtcttca 
      agatgattc gtcaatgga cgagcatgg cgttgctgc 
      tgctctttt accactttc gacaatttt ttgtcggaa 
      actttgtac ggggattat acagtttgc atatacttg 
      tattcagga gcatgcagt ttggcaaaa tcaacaatt 
      ttgggaagc tgctttcta tcaagatgt acagaaaga 
      tattaaggc tttgtatac ggcgaggtg tgaaagtgt 
      gagagggga gttgttatc gagtccaac ttcgccgag 
      agagagtaa ggattacaa ttggacgaa tcgaaggag 
      tattttgaa tcgagtcca cgaaccaac tgcgttaga 
      aatagcagc tgagcaaat gagaatctg gagttccat 
      agatgggga aaaacagaa agagttaat tacgtgcga 
      ggagaatac gatgtacag tcaagcaat tcattatgc 
      gaatcgtat ggtttattt actaattcc tttggatat 
      tggaaataa gacaagtaa acaagagtt gtttgatga 
      tgagagggc gagtaatag tatggcgaa tagtgtcgc 
      tgaaagtga cagtgtaga cgctgagtc aggtttcga 
      agatcaaga tccaggtga gactggaat gactgtaat 
      tcgtctcgt ttctcgttt catcgataa agtgtgcaa 
      tgaaggtgg tgtgacacg ggaacacat ccgaaatct 
      tcatcaaat gattcctgg agtcgtcca tatgcacat 
      tgagactct tgaagcggt ctatcgtga atcgaaacg 
      acttccacc gattcaaaa accgaaaat tctaacacc 
      aaatatgct cgccggtga agaactgat aatggaagg 
      tttgcgcgt ttatcttct tcctgatgg tagagaaga 
      aagtagtcc gggattact cggtggtcc accgcttct 
      tccagcaga gggcgccat ctttctcac aaactatcg 
      tattatttt caagggaat tccctgtga ttcgtttgc 
      ttgcgagca aatcgttgt gagatcatt tccagtgac 
      gtcgttgac taaagaaaa aaaagttgc agtgcaata 
      tttagcaca cttggatca atggttgca agagggact 
      acaggtgcg ttcgtgtac atttcaatt gatgaaatt 
      ggcgtttga cgaagaagt taccgctga aaatgtcga 
      aacattgcg aaagtttat tcataaaat tcggcatcc 
      gcccgatgt ttttcacca ttttgcgtt tacgggcca 
      agttgttgt aagtcagac accattgca taaaggcaa 
      ggagaaaaa tgcaacttt gagaggatt tgcaaagaa 
      aactttatt aaaaacggc tcgcaaagc aggatttaa 
      gcagaaatc gtcatcaaa acgtcaaaa atatgtttt 
      accaaattc gactcttta taatagtaa taaatatat 
      gacgagtcc aggtcggat gagtttgcc aaataccga 
      tgagtatag tcatgatga tgatttgtc aattgatga 
      ttttgaaac gggaccatc atcagtgcc ttcagcacc 
      cactgatgc aaagacttt ggaacgaat ggttgaacg 
      tagttatgt gagagattg gcaacgatt gggtttatg 
      taacgataa ttcctcacc gacaaatgc taaaccagc 
      tccagatca gttccgatt aactacagt taactccat 
      gtacatgat gtgtcgcag ttatccggc actgttggt 
      ggtacctgc aaatgtaac cgatgagag tattcgtcg 
      cttttgtcg ttgttaccg ccagggtag aattcctag 
      caccacttg gagacaccc tcgaacaaa agctctctt 
      attacgagg cgctggtca ccatggtaa aagtgtaat 
      gggaatgtt gaaaagtca tcccacacc aaatgcctc 
      cactgaaac cacatcatc cttcgaaca agaaagata 
      tctttcggc tctcgtttc agccactcc aatattctc 
      tctacgtca tggctcagc ttggggcat gtctgatag 
      ttctctaag tgtaaattc gttgtttct agcagctga 
      tgatccctt aggtggcgc aacgctaac acctgaagt 
      agcccgacg ttcaaaacc tctcaaagg aatgggaac 
      tttggcaat gtttcctcg gtcgtccgg tggtaaagg 
      accaaaaaa tttcggtcg ttggggttc tttgaaaga 
      cagaagaca gagttcaca agcatctct tgccaacgt 
      tcagcaaaa tcgcggcaa tatcaggca ttcaacaga 
      tgccgaaag tgcaattta tggtgttca tacgctgca 
      aagagctgc attgtatat tttgggaga gaaggcaca 
      aatgaaagg tgtgaaagt ggaatccgc tccaaagac 
      tgattttat tccagtgga gtattacga tgtgagaca 
      tacaaaaac agcatttaa aaaattgat gagggcgtg 
      cattccgag ttcggcaac ggatgcacc cgaacaaag 
      ctttcacag actaattga aaattccga atggctgca 
      acaaattca gaatataat gcagctggc tggtgccgt 
      tgtcgatct tcttgacct tcagggctc ctctgtagc 
      catttgtct ggaagacgg ttgggatac aacagctca 
      agtctcttc cgttgctca actctgtct cgatcctta 
      ctatcgcac acttgaagg ttttcgtgt tctagtcga 
      aaaagaatg gatcggttt tggacaccg tttcagcca 
      tcgtagtaa tctcaacgc gaattcaca agcgtcagg 
      tttcgcacc tacttttct tcaattttt ggatgtcgt 
      ccatcaaat tcaaaaaca atttcccat ggctttcga 
      attcagcga tttttatct acgtttttt ggcttatca 
      ttcggtttc gtgtcgttt tcgtacgtt tttattcga 
      ttgcgaatt agagagagt cgaaagtgg ggttgctgc 
      agttgagga taagagagg ttcattaac ttcgcatca 
      taaaagtgt ggacaccat ctccgatga tgagaatat 
      ttatccggg tggaagggt cgcagggac gacgagttg 
      tggtggcac aaatttggg acaaagtgt gtttgatta 
      tgtggagaa gcaacatgc taggacccc gttgttttt 
      caattttat gtacacacc ggattttga acaccctgt 
      tttgaggcc acagtcaca tttaccatg tctcgaaat 
      atggcaata ctatttgga tgagaatct ccatcatgg 
      acctgaata tgatcttga aattataca gcttgatac 
      acaacaaga agaagaggc ggaagccgc tgatggcat 
      ttcgagtaa aagtaatcg aaaaataat aacactagg 
      ttatgattg tgttagtaa atgtgtgcc agacgcatt 
      cagtcattt attggagga aatacatcg actcgaaac 
      agaactcgg acatttacc acaaaaatg gaaagtcct 
      ttgggataa actcgaatt gccaactac cgattctct 
      tactagaca tgcctcgtt tagtacagc cctagttcg 
      ttctcatgg tcgtttggt acacaaacg ttcaaccct 
      tgaaatttt gatgagggg taaaatggt gggtgctgc 
      cgaaacgtc attggtgta ttcacatcc tcatcgttt 
      cgaaaaata taattacac cacgcccac atattgtga 
      ttattgtac aaatttgtt atggggacc agtgaagac 
      cggtatgag gtgtatgga ttgtgggta cagttgcca 
      cgagaaatg tatagaagg cgtgccgaa aaattgcac 
      gaaatataa agcagtcac tgacaataa cgcatcaca 
      gacaatttc gggtacagg aggtgataa cgggtcggt 
      tagttccgg tagcgcacg ccaaacctc tagtcagca 
      atattacga tcagttttc atcgaatgt tgccgaaaa 
      tcggacaca cgaagggta tttgtataa aagaggggc 
      gttgttaaa aggctggaa gcagcgctg gtttgtttt 
      ggattcaat taaacatca gctaaggta ttacgatgc 
      gatggaaga ctcacactt taagggata tatagactt 
      agctgaagt tatgtcggt gacgtcagc tagtccagc 
      tcctggacc tccaaaaaa gactgacga caaatcatt 
      ttttgattt gcgcactag tcgacgcac ttataattt 
      ttgtgcagc agatgctgc gacagcaca agaatggat 
      cgagaagct gcaagcttg tctacaata atactctta 
      tccaaagtt actttttta gtctcatag gtgacaata 
      aatgttgaa ttattttta gtgattatt tatattttt 
      tttataaag cgcacatta tttaagtgg ctcaaaaat 
      taggtcaag tctctctat atcacgcaa gcatatcaa 
      tgtcttatt tgttattat tgttattca gtgtgtata 
      ttacattcc gtgttaata accgctcgg cattcgctt 
      cggatgtcg ccaatagcc tagagtcgc tgttatatt 
      tgtgatata ttgttaatt gtttactta cgttaacac 
      gtctcaaag tatatttcc ttagcttaa tctttgcaa 
      tgtgaaata actgcca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY