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Gene information
General information
TC007758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 397407..419877
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007758
    iBeetle screen iB_04378

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007758 RA
      MSSVLLTCS PIMLPESLD NGLRSPNNN TGLPRTRGS 
      LCLSPVTAV VDNNKRKSW CGGTPTSQF AASSRRYGE 
      LGHFKPRSD AFLEPYLLG DRQSPGMGA PTPSPPIPD 
      TPPSPPTRS ASGESLLSS NGGSEGGSE DSGSGYGAT 
      CDIGLTERL IRSHPIWFL PGIQRAGAF HLLQGKEEG 
      CFVVRQSSQ SDTMALSVR LPPDKGPYI EHYLIQSSE 
      GHLGLETSE NRFGSMPEL IAHYANCCD ELPVQLTLP 
      KAIREAKSR QQLSSLALL GQEFWRYSP PPSVASSPD 
      IDLNLMSTF KSAETKPTR PNTLNLMSF IEPLKAKNE 
      TKTPPPPPP RWSKPSTPQ NNFTVTTTV TFSVNSSPS 
      EPIEVARMS PEGQCNSTL SSKGSLILS PNSDTILSP 
      DTLSPLSAS KTTRSSKRS KHKLSKHYQ ESDIVDSPT 
      FYYRSGLGD KISDYEDVW TNNDGSKGK GLASPDILQ 
      HTNTNNNVL SPVECTTAK NPVIEDLAQ TPKQGSPFY 
      AEPADSIAN PVPRRCLAK TMVPMNQRH SNPPGLLAH 
      SPLSPGLER IDSDFNVMS SSADNLSPK QRFPGLRKP 
      EVKPVQPPL IKTRVNDSW QIDNGWTFR SSECETTME 
      SSDYDSDWP VINTLISSI HPYTPDPDD GTTVQDMIS 
      KRFPDIPTS TEELCRMSA YDNVEDRRV PRAPPSEIS 
      ELTEFSDPW TELSQVANT ETDDSINNR RSTKVASIN 
      RSKSFKDRL DPLLSAPRL QALRNREQS GPKAVGAAI 
      RTYALELAQ DKNTTFAQN IDNFIACTC ESKETNPQI 
      IMRNMRQFM SGMKNYLVK HGEKGFYKE IERERSKLK 
      STEFLNLDA ILEGVMHKL VVKPLKGHL QKLFLDHYT 
      KTGAIRLLA DNIQFAATR PIAELAIKP KITLPSESS 
      LATISQYLQ RLQSADSPL EKLEYLLAA IATIFNSVK 
      TGQLTGSGK SVQLGADDF LPLFVWVLV KTNFVAAEI 
      EAEYMWGLL HPSLLSGEG GYYLTTLSS AVHVLKNFK 
      ESCVENVKS NVKNDSVLK VVVPDELHG SILTKTLPA 
      KPHMTTKDV CNIIAHKAR ITNPQDYAL YRVTDGEET 
      MLLDNECPQ DFISGSKHT MLAYKRTDA KIAWPNQDK 
      LSL
    CDS sequence
    • >TC007758 RA
      atgtcgagc gtgctccta acatgtagt cccatcatg 
      ttgcccgaa agtctggat aacggtctc cggagtccc 
      aataataac acgggactt ccgaggacc agaggcagt 
      ctgtgtctg agtccagtg acagcagtt gtggataat 
      aataagcgt aaaagttgg tgtggtgga accccaaca 
      agtcagttc gccgcctcc agcaggaga tacggggaa 
      ttgggacac ttcaagcca cgaagcgat gccttcctg 
      gagccttac cttttgggg gatcgccag agccccggc 
      atgggggcc ccgacccct tcgccccct attccagac 
      accccaccc tcgccccca actcgttca gcttcgggt 
      gaatccctt ctatcatca aacggaggt tccgaagga 
      ggaagtgag gacagcgga tcaggttac ggcgcaact 
      tgtgacatc ggtttaacc gaacgcttg attcgctcg 
      cacccgatt tggttcctt cccggaatt cagcgagcc 
      ggtgccttc caccttttg caaggcaag gaagaaggt 
      tgtttcgta gtccgccaa tcctcccag agtgacacc 
      atggcgtta tcagtccgc ttaccgcct gataaaggc 
      ccctacatc gaacactac ctaatccag tcaagtgag 
      ggccacctc ggcctagaa acgagcgaa aatcgcttc 
      ggaagcatg cccgaactg atcgcccac tatgcgaat 
      tgttgcgac gaactcccg gtgcaatta accctcccg 
      aaggcaatc agggaggca aaatccaga caacagtta 
      tcctccctg gccctcctg ggccaggaa ttctggagg 
      tacagtccc ccaccttca gtggcttct agtccagac 
      atagattta aatctaatg agcacgttc aagtcagcc 
      gaaacgaaa cccacaaga cccaacact ttgaacttg 
      atgagtttc atcgagcct ttgaaagcg aaaaacgag 
      accaaaacg ccaccacca cccccaccc agatggtcc 
      aaaccttca accccccaa aataatttc acagttacc 
      acaacagtc acattcagt gtcaactca agtccgagt 
      gaaccaatc gaagtggcg cgaatgtcg ccggaaggg 
      cagtgcaat tcgacgctt tcctccaag ggtagttta 
      attctttca cccaactct gacaccatc ctatcgcca 
      gacacacta tcaccctta tcagcctcc aaaaccacg 
      cgcagttcc aaacgctcc aaacacaaa ctctccaaa 
      cactaccag gaaagcgat attgtcgac tcgcccacg 
      ttttactac aggagtggc ttaggggac aagatcagt 
      gactatgag gacgtctgg acgaataat gatggttcc 
      aaagggaag ggtttagct tcgcctgat attcttcaa 
      cacacgaat acgaataat aatgttctg agtccggtg 
      gaatgtaca acggccaaa aatccggtt attgaggat 
      ttggcgcaa acgcccaaa caaggcagt ccgttttat 
      gcagaacca gctgattcg attgctaat ccggtgccg 
      aggaggtgt ttggccaag acgatggtt cccatgaat 
      cagagacac tcgaatccg ccaggtttg ctggcgcat 
      tcgccgctt agtccgggt ctggagcgg attgactcg 
      gattttaac gtgatgtcg tcttctgcg gacaacctg 
      agccccaaa cagcggttt cctggctta cgcaaaccg 
      gaagtgaaa ccagtgcag cccccgttg ataaaaacc 
      cgagtgaat gatagctgg cagatcgac aatggttgg 
      acatttcga agtagtgaa tgtgagaca acaatggaa 
      tcaagcgat tacgattcc gattggccc gtcatcaat 
      acactaatt tcatcaatc cacccgtac actcccgat 
      ccggacgac gggacaacc gtccaggat atgatctcg 
      aaacggttc cccgatatt ccaacttcg accgaggaa 
      ttgtgccgg atgtcggcc tatgacaac gtcgaagac 
      cgacgagtc ccgagggct ccgccctcc gaaatcagc 
      gaactgacc gaattttcg gatccttgg accgaactc 
      agccaagtc gcaaacaca gagactgat gattcaatc 
      aacaatcgc cgcagtaca aaagtagcg tcaatcaat 
      cgctccaag agcttcaag gaccgcttg gaccccttg 
      ctctcggcc cctcgcttg caagcgctg cggaaccga 
      gagcaaagc ggccctaaa gccgtgggg gcagcgatc 
      cgaacctac gccttagaa ttagctcaa gacaaaaac 
      accacattt gctcaaaac atagacaac ttcatcgca 
      tgcacgtgc gagtcgaag gaaaccaac ccgcagata 
      atcatgcga aatatgcga cagtttatg tcagggatg 
      aagaattat ctagtgaag cacggggag aagggattt 
      tacaaggag atcgaacgg gagaggagc aaattgaaa 
      tcgacggag tttctgaat ctcgatgcg attttggaa 
      ggggttatg cacaagttg gtggtgaag ccgctgaaa 
      ggacacttg caaaagttg tttcttgat cattatacc 
      aaaacggga gcgataaga cttttagct gataatata 
      cagtttgcg gcgacgaga cccatcgcg gaattggca 
      atcaaaccg aaaattact ctgccgtcc gaaagctct 
      ctcgccacg atctcccag tacttacaa cgcctccaa 
      agtgccgac tctcctttg gaaaagctg gagtacctc 
      ctagccgca attgccaca attttcaat tcggtgaaa 
      actggccaa ttgaccggt tcgggaaaa tccgttcag 
      ttgggcgcc gacgatttc ctccccctt ttcgtgtgg 
      gttttagtc aaaacgaat ttcgtcgca gctgaaatc 
      gaagccgaa tacatgtgg gggttgctc cacccttcc 
      cttctctcg ggagaaggc ggctattat ttgaccacg 
      ttatcgtct gccgttcac gtacttaaa aacttcaaa 
      gagagttgt gtggagaac gtcaagagt aatgtaaag 
      aatgattcg gtgttgaaa gttgtggtt cctgatgag 
      ttgcacggc tcgattttg accaaaacg ttgccggcg 
      aagccacat atgaccacg aaggatgtg tgcaatatc 
      atcgcccac aaggccagg attacaaat ccccaggac 
      tatgccctt tatagggtc acagacgga gaagaaaca 
      atgttgctg gataatgag tgtccccag gattttatc 
      agcgggagc aaacacacg atgttggcc tacaaacga 
      actgacgcg aaaattgct tggcctaac caagacaaa 
      ctgagtctc tag
    mRNA sequence
    • >TC007758 RA
      ttcggttcg cgacttggc ggctgcagg attgtgctg 
      ttgttgtga acgaacgcc tgtgacacg ttggatgtg 
      tttttacta caaaatcgg tcattgtcc cggtaaatc 
      atttcatgt tggtcgata ttccacaca taaacaaac 
      cattaagga gtcagtgag tgatgtggt gaagtcgtg 
      gatttaatg tcgagcgtg ctcctaaca tgtagtccc 
      atcatgttg cccgaaagt ctggataac ggtctccgg 
      agtcccaat aataacacg ggacttccg aggaccaga 
      ggcagtctg tgtctgagt ccagtgaca gcagttgtg 
      gataataat aagcgtaaa agttggtgt ggtggaacc 
      ccaacaagt cagttcgcc gcctccagc aggagatac 
      ggggaattg ggacacttc aagccacga agcgatgcc 
      ttcctggag ccttacctt ttgggggat cgccagagc 
      cccggcatg ggggccccg accccttcg ccccctatt 
      ccagacacc ccaccctcg cccccaact cgttcagct 
      tcgggtgaa tcccttcta tcatcaaac ggaggttcc 
      gaaggagga agtgaggac agcggatca ggttacggc 
      gcaacttgt gacatcggt ttaaccgaa cgcttgatt 
      cgctcgcac ccgatttgg ttccttccc ggaattcag 
      cgagccggt gccttccac cttttgcaa ggcaaggaa 
      gaaggttgt ttcgtagtc cgccaatcc tcccagagt 
      gacaccatg gcgttatca gtccgctta ccgcctgat 
      aaaggcccc tacatcgaa cactaccta atccagtca 
      agtgagggc cacctcggc ctagaaacg agcgaaaat 
      cgcttcgga agcatgccc gaactgatc gcccactat 
      gcgaattgt tgcgacgaa ctcccggtg caattaacc 
      ctcccgaag gcaatcagg gaggcaaaa tccagacaa 
      cagttatcc tccctggcc ctcctgggc caggaattc 
      tggaggtac agtccccca ccttcagtg gcttctagt 
      ccagacata gatttaaat ctaatgagc acgttcaag 
      tcagccgaa acgaaaccc acaagaccc aacactttg 
      aacttgatg agtttcatc gagcctttg aaagcgaaa 
      aacgagacc aaaacgcca ccaccaccc ccacccaga 
      tggtccaaa ccttcaacc ccccaaaat aatttcaca 
      gttaccaca acagtcaca ttcagtgtc aactcaagt 
      ccgagtgaa ccaatcgaa gtggcgcga atgtcgccg 
      gaagggcag tgcaattcg acgctttcc tccaagggt 
      agtttaatt ctttcaccc aactctgac accatccta 
      tcgccagac acactatca cccttatca gcctccaaa 
      accacgcgc agttccaaa cgctccaaa cacaaactc 
      tccaaacac taccaggaa agcgatatt gtcgactcg 
      cccacgttt tactacagg agtggctta ggggacaag 
      atcagtgac tatgaggac gtctggacg aataatgat 
      ggttccaaa gggaagggt ttagcttcg cctgatatt 
      cttcaacac acgaatacg aataataat gttctgagt 
      ccggtggaa tgtacaacg gccaaaaat ccggttatt 
      gaggatttg gcgcaaacg cccaaacaa ggcagtccg 
      ttttatgca gaaccagct gattcgatt gctaatccg 
      gtgccgagg aggtgtttg gccaagacg atggttccc 
      atgaatcag agacactcg aatccgcca ggtttgctg 
      gcgcattcg ccgcttagt ccgggtctg gagcggatt 
      gactcggat tttaacgtg atgtcgtct tctgcggac 
      aacctgagc cccaaacag cggtttcct ggcttacgc 
      aaaccggaa gtgaaacca gtgcagccc ccgttgata 
      aaaacccga gtgaatgat agctggcag atcgacaat 
      ggttggaca tttcgaagt agtgaatgt gagacaaca 
      atggaatca agcgattac gattccgat tggcccgtc 
      atcaataca ctaatttca tcaatccac ccgtacact 
      cccgatccg gacgacggg acaaccgtc caggatatg 
      atctcgaaa cggttcccc gatattcca acttcgacc 
      gaggaattg tgccggatg tcggcctat gacaacgtc 
      gaagaccga cgagtcccg agggctccg ccctccgaa 
      atcagcgaa ctgaccgaa ttttcggat ccttggacc 
      gaactcagc caagtcgca aacacagag actgatgat 
      tcaatcaac aatcgccgc agtacaaaa gtagcgtca 
      atcaatcgc tccaagagc ttcaaggac cgcttggac 
      cccttgctc tcggcccct cgcttgcaa gcgctgcgg 
      aaccgagag caaagcggc cctaaagcc gtgggggca 
      gcgatccga acctacgcc ttagaatta gctcaagac 
      aaaaacacc acatttgct caaaacata gacaacttc 
      atcgcatgc acgtgcgag tcgaaggaa accaacccg 
      cagataatc atgcgaaat atgcgacag tttatgtca 
      gggatgaag aattatcta gtgaagcac ggggagaag 
      ggattttac aaggagatc gaacgggag aggagcaaa 
      ttgaaatcg acggagttt ctgaatctc gatgcgatt 
      ttggaaggg gttatgcac aagttggtg gtgaagccg 
      ctgaaagga cacttgcaa aagttgttt cttgatcat 
      tataccaaa acgggagcg ataagactt ttagctgat 
      aatatacag tttgcggcg acgagaccc atcgcggaa 
      ttggcaatc aaaccgaaa attactctg ccgtccgaa 
      agctctctc gccacgatc tcccagtac ttacaacgc 
      ctccaaagt gccgactct cctttggaa aagctggag 
      tacctccta gccgcaatt gccacaatt ttcaattcg 
      gtgaaaact ggccaattg accggttcg ggaaaatcc 
      gttcagttg ggcgccgac gatttcctc ccccttttc 
      gtgtgggtt ttagtcaaa acgaatttc gtcgcagct 
      gaaatcgaa gccgaatac atgtggggg ttgctccac 
      ccttccctt ctctcggga gaaggcggc tattatttg 
      accacgtta tcgtctgcc gttcacgta cttaaaaac 
      ttcaaagag agttgtgtg gagaacgtc aagagtaat 
      gtaaagaat gattcggtg ttgaaagtt gtggttcct 
      gatgagttg cacggctcg attttgacc aaaacgttg 
      ccggcgaag ccacatatg accacgaag gatgtgtgc 
      aatatcatc gcccacaag gccaggatt acaaatccc 
      caggactat gccctttat agggtcaca gacggagaa 
      gaaacaatg ttgctggat aatgagtgt ccccaggat 
      tttatcagc gggagcaaa cacacgatg ttggcctac 
      aaacgaact gacgcgaaa attgcttgg cctaaccaa 
      gacaaactg agtctctag tcatgtaca taaatttgt 
      gtttatatc aaagtgtaa cttgtcaat ttataactt 
      ttaatacac gtgttaatc tttttatac atgtagcgc 
      agctgcatc cttgtttta ttgtgtcaa taacaaatt 
      ttgtacttt ctaatttat tatctcctt ctgtactaa 
      atcatttca ataaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY