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Gene information
General information
TC007758 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 397407..419877
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007758
    iBeetle screen iB_04378

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007758 RA
      MSSVLLTCS PIMLPESLD NGLRSPNNN TGLPRTRGS 
LCLSPVTAV VDNNKRKSW CGGTPTSQF AASSRRYGE 
LGHFKPRSD AFLEPYLLG DRQSPGMGA PTPSPPIPD 
TPPSPPTRS ASGESLLSS NGGSEGGSE DSGSGYGAT 
CDIGLTERL IRSHPIWFL PGIQRAGAF HLLQGKEEG 
CFVVRQSSQ SDTMALSVR LPPDKGPYI EHYLIQSSE 
GHLGLETSE NRFGSMPEL IAHYANCCD ELPVQLTLP 
KAIREAKSR QQLSSLALL GQEFWRYSP PPSVASSPD 
IDLNLMSTF KSAETKPTR PNTLNLMSF IEPLKAKNE 
TKTPPPPPP RWSKPSTPQ NNFTVTTTV TFSVNSSPS 
EPIEVARMS PEGQCNSTL SSKGSLILS PNSDTILSP 
DTLSPLSAS KTTRSSKRS KHKLSKHYQ ESDIVDSPT 
FYYRSGLGD KISDYEDVW TNNDGSKGK GLASPDILQ 
HTNTNNNVL SPVECTTAK NPVIEDLAQ TPKQGSPFY 
AEPADSIAN PVPRRCLAK TMVPMNQRH SNPPGLLAH 
SPLSPGLER IDSDFNVMS SSADNLSPK QRFPGLRKP 
EVKPVQPPL IKTRVNDSW QIDNGWTFR SSECETTME 
SSDYDSDWP VINTLISSI HPYTPDPDD GTTVQDMIS 
KRFPDIPTS TEELCRMSA YDNVEDRRV PRAPPSEIS 
ELTEFSDPW TELSQVANT ETDDSINNR RSTKVASIN 
RSKSFKDRL DPLLSAPRL QALRNREQS GPKAVGAAI 
RTYALELAQ DKNTTFAQN IDNFIACTC ESKETNPQI 
IMRNMRQFM SGMKNYLVK HGEKGFYKE IERERSKLK 
STEFLNLDA ILEGVMHKL VVKPLKGHL QKLFLDHYT 
KTGAIRLLA DNIQFAATR PIAELAIKP KITLPSESS 
LATISQYLQ RLQSADSPL EKLEYLLAA IATIFNSVK 
TGQLTGSGK SVQLGADDF LPLFVWVLV KTNFVAAEI 
EAEYMWGLL HPSLLSGEG GYYLTTLSS AVHVLKNFK 
ESCVENVKS NVKNDSVLK VVVPDELHG SILTKTLPA 
KPHMTTKDV CNIIAHKAR ITNPQDYAL YRVTDGEET 
MLLDNECPQ DFISGSKHT MLAYKRTDA KIAWPNQDK 
LSL
    CDS sequence
    • >TC007758 RA
      atgtcgagc gtgctccta acatgtagt cccatcatg 
ttgcccgaa agtctggat aacggtctc cggagtccc 
aataataac acgggactt ccgaggacc agaggcagt 
ctgtgtctg agtccagtg acagcagtt gtggataat 
aataagcgt aaaagttgg tgtggtgga accccaaca 
agtcagttc gccgcctcc agcaggaga tacggggaa 
ttgggacac ttcaagcca cgaagcgat gccttcctg 
gagccttac cttttgggg gatcgccag agccccggc 
atgggggcc ccgacccct tcgccccct attccagac 
accccaccc tcgccccca actcgttca gcttcgggt 
gaatccctt ctatcatca aacggaggt tccgaagga 
ggaagtgag gacagcgga tcaggttac ggcgcaact 
tgtgacatc ggtttaacc gaacgcttg attcgctcg 
cacccgatt tggttcctt cccggaatt cagcgagcc 
ggtgccttc caccttttg caaggcaag gaagaaggt 
tgtttcgta gtccgccaa tcctcccag agtgacacc 
atggcgtta tcagtccgc ttaccgcct gataaaggc 
ccctacatc gaacactac ctaatccag tcaagtgag 
ggccacctc ggcctagaa acgagcgaa aatcgcttc 
ggaagcatg cccgaactg atcgcccac tatgcgaat 
tgttgcgac gaactcccg gtgcaatta accctcccg 
aaggcaatc agggaggca aaatccaga caacagtta 
tcctccctg gccctcctg ggccaggaa ttctggagg 
tacagtccc ccaccttca gtggcttct agtccagac 
atagattta aatctaatg agcacgttc aagtcagcc 
gaaacgaaa cccacaaga cccaacact ttgaacttg 
atgagtttc atcgagcct ttgaaagcg aaaaacgag 
accaaaacg ccaccacca cccccaccc agatggtcc 
aaaccttca accccccaa aataatttc acagttacc 
acaacagtc acattcagt gtcaactca agtccgagt 
gaaccaatc gaagtggcg cgaatgtcg ccggaaggg 
cagtgcaat tcgacgctt tcctccaag ggtagttta 
attctttca cccaactct gacaccatc ctatcgcca 
gacacacta tcaccctta tcagcctcc aaaaccacg 
cgcagttcc aaacgctcc aaacacaaa ctctccaaa 
cactaccag gaaagcgat attgtcgac tcgcccacg 
ttttactac aggagtggc ttaggggac aagatcagt 
gactatgag gacgtctgg acgaataat gatggttcc 
aaagggaag ggtttagct tcgcctgat attcttcaa 
cacacgaat acgaataat aatgttctg agtccggtg 
gaatgtaca acggccaaa aatccggtt attgaggat 
ttggcgcaa acgcccaaa caaggcagt ccgttttat 
gcagaacca gctgattcg attgctaat ccggtgccg 
aggaggtgt ttggccaag acgatggtt cccatgaat 
cagagacac tcgaatccg ccaggtttg ctggcgcat 
tcgccgctt agtccgggt ctggagcgg attgactcg 
gattttaac gtgatgtcg tcttctgcg gacaacctg 
agccccaaa cagcggttt cctggctta cgcaaaccg 
gaagtgaaa ccagtgcag cccccgttg ataaaaacc 
cgagtgaat gatagctgg cagatcgac aatggttgg 
acatttcga agtagtgaa tgtgagaca acaatggaa 
tcaagcgat tacgattcc gattggccc gtcatcaat 
acactaatt tcatcaatc cacccgtac actcccgat 
ccggacgac gggacaacc gtccaggat atgatctcg 
aaacggttc cccgatatt ccaacttcg accgaggaa 
ttgtgccgg atgtcggcc tatgacaac gtcgaagac 
cgacgagtc ccgagggct ccgccctcc gaaatcagc 
gaactgacc gaattttcg gatccttgg accgaactc 
agccaagtc gcaaacaca gagactgat gattcaatc 
aacaatcgc cgcagtaca aaagtagcg tcaatcaat 
cgctccaag agcttcaag gaccgcttg gaccccttg 
ctctcggcc cctcgcttg caagcgctg cggaaccga 
gagcaaagc ggccctaaa gccgtgggg gcagcgatc 
cgaacctac gccttagaa ttagctcaa gacaaaaac 
accacattt gctcaaaac atagacaac ttcatcgca 
tgcacgtgc gagtcgaag gaaaccaac ccgcagata 
atcatgcga aatatgcga cagtttatg tcagggatg 
aagaattat ctagtgaag cacggggag aagggattt 
tacaaggag atcgaacgg gagaggagc aaattgaaa 
tcgacggag tttctgaat ctcgatgcg attttggaa 
ggggttatg cacaagttg gtggtgaag ccgctgaaa 
ggacacttg caaaagttg tttcttgat cattatacc 
aaaacggga gcgataaga cttttagct gataatata 
cagtttgcg gcgacgaga cccatcgcg gaattggca 
atcaaaccg aaaattact ctgccgtcc gaaagctct 
ctcgccacg atctcccag tacttacaa cgcctccaa 
agtgccgac tctcctttg gaaaagctg gagtacctc 
ctagccgca attgccaca attttcaat tcggtgaaa 
actggccaa ttgaccggt tcgggaaaa tccgttcag 
ttgggcgcc gacgatttc ctccccctt ttcgtgtgg 
gttttagtc aaaacgaat ttcgtcgca gctgaaatc 
gaagccgaa tacatgtgg gggttgctc cacccttcc 
cttctctcg ggagaaggc ggctattat ttgaccacg 
ttatcgtct gccgttcac gtacttaaa aacttcaaa 
gagagttgt gtggagaac gtcaagagt aatgtaaag 
aatgattcg gtgttgaaa gttgtggtt cctgatgag 
ttgcacggc tcgattttg accaaaacg ttgccggcg 
aagccacat atgaccacg aaggatgtg tgcaatatc 
atcgcccac aaggccagg attacaaat ccccaggac 
tatgccctt tatagggtc acagacgga gaagaaaca 
atgttgctg gataatgag tgtccccag gattttatc 
agcgggagc aaacacacg atgttggcc tacaaacga 
actgacgcg aaaattgct tggcctaac caagacaaa 
ctgagtctc tag
    mRNA sequence
    • >TC007758 RA
      ttcggttcg cgacttggc ggctgcagg attgtgctg 
ttgttgtga acgaacgcc tgtgacacg ttggatgtg 
tttttacta caaaatcgg tcattgtcc cggtaaatc 
atttcatgt tggtcgata ttccacaca taaacaaac 
cattaagga gtcagtgag tgatgtggt gaagtcgtg 
gatttaatg tcgagcgtg ctcctaaca tgtagtccc 
atcatgttg cccgaaagt ctggataac ggtctccgg 
agtcccaat aataacacg ggacttccg aggaccaga 
ggcagtctg tgtctgagt ccagtgaca gcagttgtg 
gataataat aagcgtaaa agttggtgt ggtggaacc 
ccaacaagt cagttcgcc gcctccagc aggagatac 
ggggaattg ggacacttc aagccacga agcgatgcc 
ttcctggag ccttacctt ttgggggat cgccagagc 
cccggcatg ggggccccg accccttcg ccccctatt 
ccagacacc ccaccctcg cccccaact cgttcagct 
tcgggtgaa tcccttcta tcatcaaac ggaggttcc 
gaaggagga agtgaggac agcggatca ggttacggc 
gcaacttgt gacatcggt ttaaccgaa cgcttgatt 
cgctcgcac ccgatttgg ttccttccc ggaattcag 
cgagccggt gccttccac cttttgcaa ggcaaggaa 
gaaggttgt ttcgtagtc cgccaatcc tcccagagt 
gacaccatg gcgttatca gtccgctta ccgcctgat 
aaaggcccc tacatcgaa cactaccta atccagtca 
agtgagggc cacctcggc ctagaaacg agcgaaaat 
cgcttcgga agcatgccc gaactgatc gcccactat 
gcgaattgt tgcgacgaa ctcccggtg caattaacc 
ctcccgaag gcaatcagg gaggcaaaa tccagacaa 
cagttatcc tccctggcc ctcctgggc caggaattc 
tggaggtac agtccccca ccttcagtg gcttctagt 
ccagacata gatttaaat ctaatgagc acgttcaag 
tcagccgaa acgaaaccc acaagaccc aacactttg 
aacttgatg agtttcatc gagcctttg aaagcgaaa 
aacgagacc aaaacgcca ccaccaccc ccacccaga 
tggtccaaa ccttcaacc ccccaaaat aatttcaca 
gttaccaca acagtcaca ttcagtgtc aactcaagt 
ccgagtgaa ccaatcgaa gtggcgcga atgtcgccg 
gaagggcag tgcaattcg acgctttcc tccaagggt 
agtttaatt ctttcaccc aactctgac accatccta 
tcgccagac acactatca cccttatca gcctccaaa 
accacgcgc agttccaaa cgctccaaa cacaaactc 
tccaaacac taccaggaa agcgatatt gtcgactcg 
cccacgttt tactacagg agtggctta ggggacaag 
atcagtgac tatgaggac gtctggacg aataatgat 
ggttccaaa gggaagggt ttagcttcg cctgatatt 
cttcaacac acgaatacg aataataat gttctgagt 
ccggtggaa tgtacaacg gccaaaaat ccggttatt 
gaggatttg gcgcaaacg cccaaacaa ggcagtccg 
ttttatgca gaaccagct gattcgatt gctaatccg 
gtgccgagg aggtgtttg gccaagacg atggttccc 
atgaatcag agacactcg aatccgcca ggtttgctg 
gcgcattcg ccgcttagt ccgggtctg gagcggatt 
gactcggat tttaacgtg atgtcgtct tctgcggac 
aacctgagc cccaaacag cggtttcct ggcttacgc 
aaaccggaa gtgaaacca gtgcagccc ccgttgata 
aaaacccga gtgaatgat agctggcag atcgacaat 
ggttggaca tttcgaagt agtgaatgt gagacaaca 
atggaatca agcgattac gattccgat tggcccgtc 
atcaataca ctaatttca tcaatccac ccgtacact 
cccgatccg gacgacggg acaaccgtc caggatatg 
atctcgaaa cggttcccc gatattcca acttcgacc 
gaggaattg tgccggatg tcggcctat gacaacgtc 
gaagaccga cgagtcccg agggctccg ccctccgaa 
atcagcgaa ctgaccgaa ttttcggat ccttggacc 
gaactcagc caagtcgca aacacagag actgatgat 
tcaatcaac aatcgccgc agtacaaaa gtagcgtca 
atcaatcgc tccaagagc ttcaaggac cgcttggac 
cccttgctc tcggcccct cgcttgcaa gcgctgcgg 
aaccgagag caaagcggc cctaaagcc gtgggggca 
gcgatccga acctacgcc ttagaatta gctcaagac 
aaaaacacc acatttgct caaaacata gacaacttc 
atcgcatgc acgtgcgag tcgaaggaa accaacccg 
cagataatc atgcgaaat atgcgacag tttatgtca 
gggatgaag aattatcta gtgaagcac ggggagaag 
ggattttac aaggagatc gaacgggag aggagcaaa 
ttgaaatcg acggagttt ctgaatctc gatgcgatt 
ttggaaggg gttatgcac aagttggtg gtgaagccg 
ctgaaagga cacttgcaa aagttgttt cttgatcat 
tataccaaa acgggagcg ataagactt ttagctgat 
aatatacag tttgcggcg acgagaccc atcgcggaa 
ttggcaatc aaaccgaaa attactctg ccgtccgaa 
agctctctc gccacgatc tcccagtac ttacaacgc 
ctccaaagt gccgactct cctttggaa aagctggag 
tacctccta gccgcaatt gccacaatt ttcaattcg 
gtgaaaact ggccaattg accggttcg ggaaaatcc 
gttcagttg ggcgccgac gatttcctc ccccttttc 
gtgtgggtt ttagtcaaa acgaatttc gtcgcagct 
gaaatcgaa gccgaatac atgtggggg ttgctccac 
ccttccctt ctctcggga gaaggcggc tattatttg 
accacgtta tcgtctgcc gttcacgta cttaaaaac 
ttcaaagag agttgtgtg gagaacgtc aagagtaat 
gtaaagaat gattcggtg ttgaaagtt gtggttcct 
gatgagttg cacggctcg attttgacc aaaacgttg 
ccggcgaag ccacatatg accacgaag gatgtgtgc 
aatatcatc gcccacaag gccaggatt acaaatccc 
caggactat gccctttat agggtcaca gacggagaa 
gaaacaatg ttgctggat aatgagtgt ccccaggat 
tttatcagc gggagcaaa cacacgatg ttggcctac 
aaacgaact gacgcgaaa attgcttgg cctaaccaa 
gacaaactg agtctctag tcatgtaca taaatttgt 
gtttatatc aaagtgtaa cttgtcaat ttataactt 
ttaatacac gtgttaatc tttttatac atgtagcgc 
agctgcatc cttgtttta ttgtgtcaa taacaaatt 
ttgtacttt ctaatttat tatctcctt ctgtactaa 
atcatttca ataaaa
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