| General information | |||
| TC007758 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 397407..419877 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007758 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04378 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC007758 RA
MSSVLLTCS PIMLPESLD NGLRSPNNN TGLPRTRGS LCLSPVTAV VDNNKRKSW CGGTPTSQF AASSRRYGE LGHFKPRSD AFLEPYLLG DRQSPGMGA PTPSPPIPD TPPSPPTRS ASGESLLSS NGGSEGGSE DSGSGYGAT CDIGLTERL IRSHPIWFL PGIQRAGAF HLLQGKEEG CFVVRQSSQ SDTMALSVR LPPDKGPYI EHYLIQSSE GHLGLETSE NRFGSMPEL IAHYANCCD ELPVQLTLP KAIREAKSR QQLSSLALL GQEFWRYSP PPSVASSPD IDLNLMSTF KSAETKPTR PNTLNLMSF IEPLKAKNE TKTPPPPPP RWSKPSTPQ NNFTVTTTV TFSVNSSPS EPIEVARMS PEGQCNSTL SSKGSLILS PNSDTILSP DTLSPLSAS KTTRSSKRS KHKLSKHYQ ESDIVDSPT FYYRSGLGD KISDYEDVW TNNDGSKGK GLASPDILQ HTNTNNNVL SPVECTTAK NPVIEDLAQ TPKQGSPFY AEPADSIAN PVPRRCLAK TMVPMNQRH SNPPGLLAH SPLSPGLER IDSDFNVMS SSADNLSPK QRFPGLRKP EVKPVQPPL IKTRVNDSW QIDNGWTFR SSECETTME SSDYDSDWP VINTLISSI HPYTPDPDD GTTVQDMIS KRFPDIPTS TEELCRMSA YDNVEDRRV PRAPPSEIS ELTEFSDPW TELSQVANT ETDDSINNR RSTKVASIN RSKSFKDRL DPLLSAPRL QALRNREQS GPKAVGAAI RTYALELAQ DKNTTFAQN IDNFIACTC ESKETNPQI IMRNMRQFM SGMKNYLVK HGEKGFYKE IERERSKLK STEFLNLDA ILEGVMHKL VVKPLKGHL QKLFLDHYT KTGAIRLLA DNIQFAATR PIAELAIKP KITLPSESS LATISQYLQ RLQSADSPL EKLEYLLAA IATIFNSVK TGQLTGSGK SVQLGADDF LPLFVWVLV KTNFVAAEI EAEYMWGLL HPSLLSGEG GYYLTTLSS AVHVLKNFK ESCVENVKS NVKNDSVLK VVVPDELHG SILTKTLPA KPHMTTKDV CNIIAHKAR ITNPQDYAL YRVTDGEET MLLDNECPQ DFISGSKHT MLAYKRTDA KIAWPNQDK LSL
- >TC007758 RA
atgtcgagc gtgctccta acatgtagt cccatcatg ttgcccgaa agtctggat aacggtctc cggagtccc aataataac acgggactt ccgaggacc agaggcagt ctgtgtctg agtccagtg acagcagtt gtggataat aataagcgt aaaagttgg tgtggtgga accccaaca agtcagttc gccgcctcc agcaggaga tacggggaa ttgggacac ttcaagcca cgaagcgat gccttcctg gagccttac cttttgggg gatcgccag agccccggc atgggggcc ccgacccct tcgccccct attccagac accccaccc tcgccccca actcgttca gcttcgggt gaatccctt ctatcatca aacggaggt tccgaagga ggaagtgag gacagcgga tcaggttac ggcgcaact tgtgacatc ggtttaacc gaacgcttg attcgctcg cacccgatt tggttcctt cccggaatt cagcgagcc ggtgccttc caccttttg caaggcaag gaagaaggt tgtttcgta gtccgccaa tcctcccag agtgacacc atggcgtta tcagtccgc ttaccgcct gataaaggc ccctacatc gaacactac ctaatccag tcaagtgag ggccacctc ggcctagaa acgagcgaa aatcgcttc ggaagcatg cccgaactg atcgcccac tatgcgaat tgttgcgac gaactcccg gtgcaatta accctcccg aaggcaatc agggaggca aaatccaga caacagtta tcctccctg gccctcctg ggccaggaa ttctggagg tacagtccc ccaccttca gtggcttct agtccagac atagattta aatctaatg agcacgttc aagtcagcc gaaacgaaa cccacaaga cccaacact ttgaacttg atgagtttc atcgagcct ttgaaagcg aaaaacgag accaaaacg ccaccacca cccccaccc agatggtcc aaaccttca accccccaa aataatttc acagttacc acaacagtc acattcagt gtcaactca agtccgagt gaaccaatc gaagtggcg cgaatgtcg ccggaaggg cagtgcaat tcgacgctt tcctccaag ggtagttta attctttca cccaactct gacaccatc ctatcgcca gacacacta tcaccctta tcagcctcc aaaaccacg cgcagttcc aaacgctcc aaacacaaa ctctccaaa cactaccag gaaagcgat attgtcgac tcgcccacg ttttactac aggagtggc ttaggggac aagatcagt gactatgag gacgtctgg acgaataat gatggttcc aaagggaag ggtttagct tcgcctgat attcttcaa cacacgaat acgaataat aatgttctg agtccggtg gaatgtaca acggccaaa aatccggtt attgaggat ttggcgcaa acgcccaaa caaggcagt ccgttttat gcagaacca gctgattcg attgctaat ccggtgccg aggaggtgt ttggccaag acgatggtt cccatgaat cagagacac tcgaatccg ccaggtttg ctggcgcat tcgccgctt agtccgggt ctggagcgg attgactcg gattttaac gtgatgtcg tcttctgcg gacaacctg agccccaaa cagcggttt cctggctta cgcaaaccg gaagtgaaa ccagtgcag cccccgttg ataaaaacc cgagtgaat gatagctgg cagatcgac aatggttgg acatttcga agtagtgaa tgtgagaca acaatggaa tcaagcgat tacgattcc gattggccc gtcatcaat acactaatt tcatcaatc cacccgtac actcccgat ccggacgac gggacaacc gtccaggat atgatctcg aaacggttc cccgatatt ccaacttcg accgaggaa ttgtgccgg atgtcggcc tatgacaac gtcgaagac cgacgagtc ccgagggct ccgccctcc gaaatcagc gaactgacc gaattttcg gatccttgg accgaactc agccaagtc gcaaacaca gagactgat gattcaatc aacaatcgc cgcagtaca aaagtagcg tcaatcaat cgctccaag agcttcaag gaccgcttg gaccccttg ctctcggcc cctcgcttg caagcgctg cggaaccga gagcaaagc ggccctaaa gccgtgggg gcagcgatc cgaacctac gccttagaa ttagctcaa gacaaaaac accacattt gctcaaaac atagacaac ttcatcgca tgcacgtgc gagtcgaag gaaaccaac ccgcagata atcatgcga aatatgcga cagtttatg tcagggatg aagaattat ctagtgaag cacggggag aagggattt tacaaggag atcgaacgg gagaggagc aaattgaaa tcgacggag tttctgaat ctcgatgcg attttggaa ggggttatg cacaagttg gtggtgaag ccgctgaaa ggacacttg caaaagttg tttcttgat cattatacc aaaacggga gcgataaga cttttagct gataatata cagtttgcg gcgacgaga cccatcgcg gaattggca atcaaaccg aaaattact ctgccgtcc gaaagctct ctcgccacg atctcccag tacttacaa cgcctccaa agtgccgac tctcctttg gaaaagctg gagtacctc ctagccgca attgccaca attttcaat tcggtgaaa actggccaa ttgaccggt tcgggaaaa tccgttcag ttgggcgcc gacgatttc ctccccctt ttcgtgtgg gttttagtc aaaacgaat ttcgtcgca gctgaaatc gaagccgaa tacatgtgg gggttgctc cacccttcc cttctctcg ggagaaggc ggctattat ttgaccacg ttatcgtct gccgttcac gtacttaaa aacttcaaa gagagttgt gtggagaac gtcaagagt aatgtaaag aatgattcg gtgttgaaa gttgtggtt cctgatgag ttgcacggc tcgattttg accaaaacg ttgccggcg aagccacat atgaccacg aaggatgtg tgcaatatc atcgcccac aaggccagg attacaaat ccccaggac tatgccctt tatagggtc acagacgga gaagaaaca atgttgctg gataatgag tgtccccag gattttatc agcgggagc aaacacacg atgttggcc tacaaacga actgacgcg aaaattgct tggcctaac caagacaaa ctgagtctc tag
- >TC007758 RA
ttcggttcg cgacttggc ggctgcagg attgtgctg ttgttgtga acgaacgcc tgtgacacg ttggatgtg tttttacta caaaatcgg tcattgtcc cggtaaatc atttcatgt tggtcgata ttccacaca taaacaaac cattaagga gtcagtgag tgatgtggt gaagtcgtg gatttaatg tcgagcgtg ctcctaaca tgtagtccc atcatgttg cccgaaagt ctggataac ggtctccgg agtcccaat aataacacg ggacttccg aggaccaga ggcagtctg tgtctgagt ccagtgaca gcagttgtg gataataat aagcgtaaa agttggtgt ggtggaacc ccaacaagt cagttcgcc gcctccagc aggagatac ggggaattg ggacacttc aagccacga agcgatgcc ttcctggag ccttacctt ttgggggat cgccagagc cccggcatg ggggccccg accccttcg ccccctatt ccagacacc ccaccctcg cccccaact cgttcagct tcgggtgaa tcccttcta tcatcaaac ggaggttcc gaaggagga agtgaggac agcggatca ggttacggc gcaacttgt gacatcggt ttaaccgaa cgcttgatt cgctcgcac ccgatttgg ttccttccc ggaattcag cgagccggt gccttccac cttttgcaa ggcaaggaa gaaggttgt ttcgtagtc cgccaatcc tcccagagt gacaccatg gcgttatca gtccgctta ccgcctgat aaaggcccc tacatcgaa cactaccta atccagtca agtgagggc cacctcggc ctagaaacg agcgaaaat cgcttcgga agcatgccc gaactgatc gcccactat gcgaattgt tgcgacgaa ctcccggtg caattaacc ctcccgaag gcaatcagg gaggcaaaa tccagacaa cagttatcc tccctggcc ctcctgggc caggaattc tggaggtac agtccccca ccttcagtg gcttctagt ccagacata gatttaaat ctaatgagc acgttcaag tcagccgaa acgaaaccc acaagaccc aacactttg aacttgatg agtttcatc gagcctttg aaagcgaaa aacgagacc aaaacgcca ccaccaccc ccacccaga tggtccaaa ccttcaacc ccccaaaat aatttcaca gttaccaca acagtcaca ttcagtgtc aactcaagt ccgagtgaa ccaatcgaa gtggcgcga atgtcgccg gaagggcag tgcaattcg acgctttcc tccaagggt agtttaatt ctttcaccc aactctgac accatccta tcgccagac acactatca cccttatca gcctccaaa accacgcgc agttccaaa cgctccaaa cacaaactc tccaaacac taccaggaa agcgatatt gtcgactcg cccacgttt tactacagg agtggctta ggggacaag atcagtgac tatgaggac gtctggacg aataatgat ggttccaaa gggaagggt ttagcttcg cctgatatt cttcaacac acgaatacg aataataat gttctgagt ccggtggaa tgtacaacg gccaaaaat ccggttatt gaggatttg gcgcaaacg cccaaacaa ggcagtccg ttttatgca gaaccagct gattcgatt gctaatccg gtgccgagg aggtgtttg gccaagacg atggttccc atgaatcag agacactcg aatccgcca ggtttgctg gcgcattcg ccgcttagt ccgggtctg gagcggatt gactcggat tttaacgtg atgtcgtct tctgcggac aacctgagc cccaaacag cggtttcct ggcttacgc aaaccggaa gtgaaacca gtgcagccc ccgttgata aaaacccga gtgaatgat agctggcag atcgacaat ggttggaca tttcgaagt agtgaatgt gagacaaca atggaatca agcgattac gattccgat tggcccgtc atcaataca ctaatttca tcaatccac ccgtacact cccgatccg gacgacggg acaaccgtc caggatatg atctcgaaa cggttcccc gatattcca acttcgacc gaggaattg tgccggatg tcggcctat gacaacgtc gaagaccga cgagtcccg agggctccg ccctccgaa atcagcgaa ctgaccgaa ttttcggat ccttggacc gaactcagc caagtcgca aacacagag actgatgat tcaatcaac aatcgccgc agtacaaaa gtagcgtca atcaatcgc tccaagagc ttcaaggac cgcttggac cccttgctc tcggcccct cgcttgcaa gcgctgcgg aaccgagag caaagcggc cctaaagcc gtgggggca gcgatccga acctacgcc ttagaatta gctcaagac aaaaacacc acatttgct caaaacata gacaacttc atcgcatgc acgtgcgag tcgaaggaa accaacccg cagataatc atgcgaaat atgcgacag tttatgtca gggatgaag aattatcta gtgaagcac ggggagaag ggattttac aaggagatc gaacgggag aggagcaaa ttgaaatcg acggagttt ctgaatctc gatgcgatt ttggaaggg gttatgcac aagttggtg gtgaagccg ctgaaagga cacttgcaa aagttgttt cttgatcat tataccaaa acgggagcg ataagactt ttagctgat aatatacag tttgcggcg acgagaccc atcgcggaa ttggcaatc aaaccgaaa attactctg ccgtccgaa agctctctc gccacgatc tcccagtac ttacaacgc ctccaaagt gccgactct cctttggaa aagctggag tacctccta gccgcaatt gccacaatt ttcaattcg gtgaaaact ggccaattg accggttcg ggaaaatcc gttcagttg ggcgccgac gatttcctc ccccttttc gtgtgggtt ttagtcaaa acgaatttc gtcgcagct gaaatcgaa gccgaatac atgtggggg ttgctccac ccttccctt ctctcggga gaaggcggc tattatttg accacgtta tcgtctgcc gttcacgta cttaaaaac ttcaaagag agttgtgtg gagaacgtc aagagtaat gtaaagaat gattcggtg ttgaaagtt gtggttcct gatgagttg cacggctcg attttgacc aaaacgttg ccggcgaag ccacatatg accacgaag gatgtgtgc aatatcatc gcccacaag gccaggatt acaaatccc caggactat gccctttat agggtcaca gacggagaa gaaacaatg ttgctggat aatgagtgt ccccaggat tttatcagc gggagcaaa cacacgatg ttggcctac aaacgaact gacgcgaaa attgcttgg cctaaccaa gacaaactg agtctctag tcatgtaca taaatttgt gtttatatc aaagtgtaa cttgtcaat ttataactt ttaatacac gtgttaatc tttttatac atgtagcgc agctgcatc cttgtttta ttgtgtcaa taacaaatt ttgtacttt ctaatttat tatctcctt ctgtactaa atcatttca ataaaa
