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Gene information
General information
TC007751 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 504111..509856
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007751
    iBeetle screen iB_04377

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007751 RA
      MHKITKGLK KKKKGKKSK HKEEELFKP EELEAYRRE 
      HQESGASSG QNEEWKKFI QLTSGVDDI LKKTQGDLD 
      RIKSTSFFQ RVPPPSQVK EAAEAEEEK AKEAEQQVV 
      EENKQTAET NLGIVQVSE SESEEEEDD DIFDTAYID 
      EVKLAYIPE SPSEETTGD DPFDTSIAE RAILGPAVE 
      RKGKKLVPI GAAVEVLTG RVQIPTCAT PRPSSNRRQ 
      VLKEKNLLL GSFDDNANS IQTNQETGS EEVPKTLLD 
      EDPPTPLSE PIDLGKTLH QIVIPDEDD EEFAKLAAE 
      SLAKSPVSV VAVPSDQVV VPEENWAAF SEEKAESPK 
      DPFDTSFAE NILPGKAEL KIIENEILN ESNDLDFDP 
      RAEDKLSQI INKVSIKVT DPAGQRESI SSLDRVSET 
      DLNLITPTH RDLLGGSNT DLSNLNEQP LRPCEQTPS 
      ADYIEYCDP FDTSIVETV QLPGQTELK FLEKELLGD 
      LEDEFDPRA GEPKERTIS RPDVLNIGS KTVSFDLPS 
      PQNELLEVR EKIAKPLTP FYVRKNSVP EEFFPEDDP 
      FDTSFVSEI KPGKAELKL IESELEQLE ENFDFDPRK 
      EEQKIVQVV KEPVDLLAV EHQVPAKVL TPLEEKTEI 
      SYTDPFDTS IVNICPGKT ELKLLESEL IESGQTPVD 
      LLDNFPDVA SHTCLKPEN FDSDPFDTS IANNIAPGK 
      TELKLLESE LI
    CDS sequence
    • >TC007751 RA
      atgcacaag ataacaaaa ggcctgaaa aagaagaaa 
      aagggcaaa aaatctaag cacaaagaa gaggagctt 
      ttcaagccg gaagaactc gaagcatac cgtcgcgaa 
      caccaagaa tccggcgct tcttcaggt caaaacgaa 
      gagtggaag aaattcatc caactcaca tcaggcgtt 
      gacgatatc ttaaaaaaa acccaaggc gacttagac 
      cgcatcaaa agcacttca ttttttcaa cgcgttccg 
      ccacccagt caagtcaaa gaagcagca gaggcagaa 
      gaagaaaaa gcaaaagaa gcggaacaa caagttgtt 
      gaagaaaac aaacaaaca gccgaaacc aatcttggc 
      attgttcaa gtctccgaa tccgaatcg gaagaagag 
      gaagatgat gatattttc gacactgcg tacattgat 
      gaagtaaaa ctagcttac atcccggaa tcaccaagt 
      gaggaaacc accggtgat gaccctttt gacacctcc 
      atagctgag agggcgatc ttgggacct gcagtcgaa 
      cgcaagggc aaaaaacta gttccgatt ggagcagct 
      gttgaggtt ttaacagga agggttcaa ataccaacg 
      tgtgcaaca ccaagaccg agctcaaat cgaagacaa 
      gtccttaag gagaaaaac ctactgttg gggagtttt 
      gacgataac gccaattcg attcagaca aatcaagag 
      actggtagt gaagaggtt cctaaaact ttactcgat 
      gaagatccg ccgactcca ctaagtgaa ccaattgat 
      ttgggcaag actttacac caaattgtt attccagat 
      gaagatgat gaagaattt gcgaaacta gcggctgag 
      agtttggcc aaaagccca gtcagtgtt gttgcggta 
      ccaagtgac caagtggtc gttcctgag gaaaactgg 
      gccgctttc agtgaagaa aaagccgaa agtccaaaa 
      gacccattc gacacaagc ttcgccgaa aacatcctt 
      ccaggcaaa gctgagcta aaaatcatc gaaaatgag 
      attttgaac gaaagcaac gatctcgac ttcgaccca 
      agagccgaa gacaagctt tcgcaaatt atcaacaaa 
      gtcagtatt aaagtaact gatcctgcg ggacaacgg 
      gaatcaatc tcgtcactt gaccgcgtt tcagaaacc 
      gatctaaac ctaatcact ccaacccac cgcgatttg 
      ctcggagga agcaacact gacttatca aacctcaac 
      gagcagccg ttacgacct tgtgaacaa actccaagt 
      gctgattac atagaatat tgcgacccg ttcgatact 
      tcaatcgtc gaaacagtc caacttcct ggacaaacc 
      gaactcaaa tttctcgaa aaggaacta ctcggtgac 
      cttgaggac gaattcgat cccagagct ggtgaacca 
      aaagaacgg acaatttcc cgccctgat gtgctaaac 
      atcggctcg aaaaccgtc tcctttgat ttgccgtca 
      cctcagaac gaactactc gaagtgcgt gaaaaaatc 
      gcaaagcct ttaactccc ttctacgtg cgcaaaaac 
      tcagtcccc gaggagttc ttcccagag gatgatccg 
      ttcgatacg tcgttcgtg agcgagatc aagccggga 
      aaagccgaa ttaaaatta attgaaagt gaattggaa 
      cagttggag gaaaatttt gattttgat ccgaggaaa 
      gaggagcaa aaaattgtt caagtggtc aaagaaccg 
      gtggatttg ttggcggtt gagcaccaa gttcccgcc 
      aaagttctg acacctttg gaggaaaaa accgagatt 
      tcgtacaca gatccgttc gatacgagc atagttaat 
      atttgtccc ggaaaaacc gaactgaaa ctactcgaa 
      agcgagttg atagaatca gggcaaact ccggttgat 
      ctactggac aatttcccg gatgttgca agtcacact 
      tgcctgaag ccggagaat ttcgacagc gatcccttc 
      gatacaagc attgccaat aacatagca cccggaaaa 
      actgagctg aaactgcta gaatccgag ctgatttga
    mRNA sequence
    • >TC007751 RA
      tttggaaag ccgtcttca agtaaaaaa tttctcaaa 
      taaatcaat tcgtgtttt aatagacaa tgtaactgt 
      tttattttt cggtgcgat ttcagtctg aatgcttga 
      tgtgatatg aggcctaaa aaaagcctc gatgagact 
      tagagtaga taatgcaca agataacaa aaggcctga 
      aaaagaaga aaaagggca aaaaatcta agcacaaag 
      aagaggagc ttttcaagc cggaagaac tcgaagcat 
      accgtcgcg aacaccaag aatccggcg cttcttcag 
      gtcaaaacg aagagtgga agaaattca tccaactca 
      catcaggcg ttgacgata tcttaaaaa aaacccaag 
      gcgacttag accgcatca aaagcactt cattttttc 
      aacgcgttc cgccaccca gtcaagtca aagaagcag 
      cagaggcag aagaagaaa aagcaaaag aagcggaac 
      aacaagttg ttgaagaaa acaaacaaa cagccgaaa 
      ccaatcttg gcattgttc aagtctccg aatccgaat 
      cggaagaag aggaagatg atgatattt tcgacactg 
      cgtacattg atgaagtaa aactagctt acatcccgg 
      aatcaccaa gtgaggaaa ccaccggtg atgaccctt 
      ttgacacct ccatagctg agagggcga tcttgggac 
      ctgcagtcg aacgcaagg gcaaaaaac tagttccga 
      ttggagcag ctgttgagg ttttaacag gaagggttc 
      aaataccaa cgtgtgcaa caccaagac cgagctcaa 
      atcgaagac aagtcctta aggagaaaa acctactgt 
      tggggagtt ttgacgata acgccaatt cgattcaga 
      caaatcaag agactggta gtgaagagg ttcctaaaa 
      ctttactcg atgaagatc cgccgactc cactaagtg 
      aaccaattg atttgggca agactttac accaaattg 
      ttattccag atgaagatg atgaagaat ttgcgaaac 
      tagcggctg agagtttgg ccaaaagcc cagtcagtg 
      ttgttgcgg taccaagtg accaagtgg tcgttcctg 
      aggaaaact gggccgctt tcagtgaag aaaaagccg 
      aaagtccaa aagacccat tcgacacaa gcttcgccg 
      aaaacatcc ttccaggca aagctgagc taaaaatca 
      tcgaaaatg agattttga acgaaagca acgatctcg 
      acttcgacc caagagccg aagacaagc tttcgcaaa 
      ttatcaaca aagtcagta ttaaagtaa ctgatcctg 
      cgggacaac gggaatcaa tctcgtcac ttgaccgcg 
      tttcagaaa ccgatctaa acctaatca ctccaaccc 
      accgcgatt tgctcggag gaagcaaca ctgacttat 
      caaacctca acgagcagc cgttacgac cttgtgaac 
      aaactccaa gtgctgatt acatagaat attgcgacc 
      cgttcgata cttcaatcg tcgaaacag tccaacttc 
      ctggacaaa ccgaactca aatttctcg aaaaggaac 
      tactcggtg accttgagg acgaattcg atcccagag 
      ctggtgaac caaaagaac ggacaattt cccgccctg 
      atgtgctaa acatcggct cgaaaaccg tctcctttg 
      atttgccgt cacctcaga acgaactac tcgaagtgc 
      gtgaaaaaa tcgcaaagc ctttaactc ccttctacg 
      tgcgcaaaa actcagtcc ccgaggagt tcttcccag 
      aggatgatc cgttcgata cgtcgttcg tgagcgaga 
      tcaagccgg gaaaagccg aattaaaat taattgaaa 
      gtgaattgg aacagttgg aggaaaatt ttgattttg 
      atccgagga aagaggagc aaaaaattg ttcaagtgg 
      tcaaagaac cggtggatt tgttggcgg ttgagcacc 
      aagttcccg ccaaagttc tgacacctt tggaggaaa 
      aaaccgaga tttcgtaca cagatccgt tcgatacga 
      gcatagtta atatttgtc ccggaaaaa ccgaactga 
      aactactcg aaagcgagt tgatagaat cagggcaaa 
      ctccggttg atctactgg acaatttcc cggatgttg 
      caagtcaca cttgcctga agccggaga atttcgaca 
      gcgatccct tcgatacaa gcattgcca ataacatag 
      cacccggaa aaactgagc tgaaactgc tagaatccg 
      agctgattt gaaatggag gtgaaaacg gggaaatcc 
      ctttctctt cactgaaga tgaatacag ttcgccaca 
      ttcg
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