General information | |||
TC007743 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 483254..488677 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC007743 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04376 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Comment: sometimes insideout |
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC007743 RA
MGDSVDVEA KVTRKTLIM PNLPKSLSI EEKKYLLAI ERGDMANVR RMLQKAHRK KSFDINCVD SLGRGALSI AIDQEHLEM VELLVIMGV ETRDALLHA IHVEFVEAV ELLLEHEEL IHKDGEPYS WQKVDINTA MFTPDITPL TLAAHRNNY EILKILLDR GATIPMPHD IKCGCDECI KESEEDYLR HSLARLNEY KALASPSLI ALSSSDPIL TAFQLSWEL RNLAFAEPE CKSEYMDLR RQCQQFAVD LLHQTRSSQ ELAFILNHD PAAPPYEEG EHMKLARLE LAIQYKQKK FVAHPNIQQ LLAAIWYEG VPGFRRKSS TQKIMIIVK VAVLFPFYC MLYMITPGT KTGKLMRKP FMKFLIHAS SYLFFLFLL ILVSQRAED QIIELFGTE SMKQNLQEK YRKQRGNPP TILEYVVLI YVIGFIFEE THEIYVEGM KSYLRNLWN FIDFTRNFL YTAVFLLRV AAYIQQTSE IENDPFTAY IRREEWQAF DPQLIAEGL FAAANIFSA LKLVHLFSI NPHLGPLQI SLGRMVIDI VKFFFIYTL VLFAFACGL NQLLWYFSD LEKKKCYHL PSGEADFDN AADSCMKWR RFANVFESS QSLFWASFG MVDLASFEL TGIKTYTRF WGLLMFGSY SVINVIVLL NLLIAMMSN SYAMIDEHS DTEWKFART KLWMSYFEE SATLPSPFN IFPKPKHLF KLLGLRKKD KIRKMSTKR RNREEKERD YKYTAVMRA LVWRYVSAM HRKLEENAV TEDDVNELK SDISSFRYE LLEVLQKNG MDISSAEMK EKAVLGRKM KVWERRLMK DFKVAPVVT EEEEEFIYQ PPPEGENSL AKFRRIARL AVVNSNLSK WRQVVKGAC IASQIGHCH SRDSFKKQQ NLQRAMEEA RKLKVKSPD QSRATTPIQ LPDTTGSNI IKIIRGITP ETVDTKYCL TPRDKSPLI SISPPKTPE KEPDLINLE SAKTSPVLP VKAMPSDDV SLESVDKII NDSNSLDDD KVRSLPTSP TSSDDKSLD SLSKKALFS SGSFTPEEN EKKEEETEP KNVTFSFDF IDESSKGES SAEILKKSE EETEENLDK SDEPEPSLE ETKEESEEV HDLPEETVQ AKSEDEVEE TENNEIEEP QADLEESEP QLDEKEEES QVKSEELTV EEKEIQVEV EKVCAKSVD ELEAPEKKE EVEEKIEKE KSPLAVRNI QRIGDRGRR QPKTGWL
- >TC007743 RA
atgggggat tcggttgat gttgaggct aaagtcaca cgcaagacc ctaatcatg cccaacttg cccaagtca ctttcaatt gaagaaaaa aaatattta ttggccatt gaacgtgga gacatggca aatgttcgc cgaatgttg caaaaagcc caccggaaa aaatcgttt gatattaat tgtgtggat agtctagga cgtggtgct ttatcgatt gccatcgac caagagcat ctcgaaatg gtggaactc ctggtgatc atgggagtg gaaactaga gatgctctt ctgcatgct attcacgta gagtttgtg gaagctgtt gagcttttg ttggaacac gaggagttg atccacaaa gatggggag ccttatagt tggcaaaaa gttgacatc aacactgcc atgttcaca cctgatatc actcccttg actttggcc gcccatcga aataattac gaaattttg aaaattttg ttagacaga ggagcgact attccgatg ccacatgat atcaaatgt ggatgtgat gaatgtatc aaagaatct gaagaggat tatttacga cactcgtta gcgagacta aacgagtac aaagctctg gcaagtccg tctttgatt gcactttcg tcaagtgat ccgatttta acagcgttt caattgtcg tgggaattg aggaatctg gcgtttgct gaaccggaa tgtaaatcc gaatacatg gacctgcgc cgccaatgc caacaattc gctgtcgac ctcctccac caaactcgc agctcccaa gaactagcg ttcatcctc aaccacgac ccagccgcc ccaccttac gaagaaggc gaacacatg aaacttgca cgcctcgaa ctcgccatt caatacaaa caaaaaaaa ttcgtagcc catccaaac atccaacaa ctcttagcc gccatctgg tacgaaggc gttcccgga tttcggcgc aaatcctca acccaaaaa atcatgatc attgtcaaa gtggccgtt ttattcccc ttctattgc atgctatac atgataaca cccggaacg aaaaccggg aaactaatg agaaaaccc ttcatgaaa ttcttaatt catgcctcg tcctatttg ttctttttg tttttgtta atcctggtg tctcaacga gccgaagac caaatcatc gaattgttc ggaaccgaa agcatgaaa caaaaccta caggaaaag taccgaaaa caaagggga aacccccca actatccta gaatatgtt gtcttgatt tatgttatt gggttcatt ttcgaggaa actcacgag atttatgtg gaaggaatg aaatcgtat ttgcgtaac ttgtggaat tttattgat tttacgcga aattttctt tatacggcc gtttttttg ttacgtgtg gctgcctat atccagcaa acttccgaa attgaaaat gaccctttt acggcatat atcaggcga gaggagtgg caagctttc gatccgcaa ttgattgct gaggggctt ttcgcagcc gcaaatatt ttcagtgcc ttaaaactg gtccatttg ttctccatc aacccacat ttggggccg ttgcaaata tcgcttgga agaatggtc atagacatt gtaaaattc ttctttatt tacactctg gtgctgttt gcttttgcc tgcggtttg aaccaactc ttgtggtac ttttcggat ttggaaaag aagaaatgt taccattta ccaagtggt gaagccgat tttgataat gcggccgat tcgtgcatg aagtggcgc cgtttcgct aacgttttt gagtcgtcc cagtcgctt ttctgggcc agcttcggt atggtggac ctcgccagt tttgagtta accggaatc aaaacttac acgagattt tggggcctt ttaatgttc ggttcttac tcggtgatc aatgtcatc gttttattg aaccttcta atagccatg atgtcaaac tcgtatgca atgatcgac gaacattca gacaccgaa tggaaattt gcacgaacc aaactatgg atgagctat ttcgaagaa agcgctact ttaccgtca cccttcaac atcttcccc aaacccaaa cacttgttc aaacttttg ggcttgcgc aagaaagac aaaatacga aaaatgtca acgaagcga agaaatcgc gaggaaaaa gaacgcgat tacaaatat actgccgtt atgcgggct ttagtttgg cgttatgtc tcagcaatg caccgaaag ctggaagaa aacgctgtg actgaagac gatgttaat gaactaaag agcgacatt tcgtcgttt cgttatgaa ttattggag gtgttgcag aaaaatggc atggatatt tcttcggct gaaatgaag gaaaaggcc gttttgggc cgaaaaatg aaagtttgg gaacgaaga ctgatgaag gactttaaa gttgcgccg gttgttacg gaagaagag gaagaattc atctaccaa ccccctcca gaaggcgaa aactcccta gccaagttt cggcgaatc gcgagatta gctgttgtt aactcaaat ttgagtaaa tggaggcag gtggtcaaa ggtgcctgc attgcgtcg cagattggc cactgccac agtcgcgac tcgttcaaa aagcagcaa aatcttcaa agagcgatg gaagaagcg cggaaattg aaagtgaaa agtccggat cagagtcgc gccacgact ccgatccaa cttccggac accaccgga tccaacatc attaaaata atccgggga atcaccccc gaaaccgtc gatacgaaa tattgccta actcccaga gacaagagt cctttgatt tccatttct ccaccgaaa acgccggaa aaagaaccg gatttgatc aacttagag agtgccaag acctcgccc gttctccca gtcaaggct atgccctct gatgatgtg tcgttggaa tcggttgat aaaattatc aacgactcc aattctcta gatgatgat aaagttaga agtttgcca acttcacca acttcgagt gacgataaa agtttggat agtttgagc aaaaaagca cttttctca tcgggaagt ttcactccc gaagaaaat gagaagaaa gaagaagaa actgagccg aaaaatgtc acttttagt tttgatttt atcgatgag agtagtaaa ggggagagt tcagccgaa atattgaag aaatcagag gaagaaact gaagaaaat ctggacaaa agtgatgaa ccggaacca tcacttgaa gaaactaag gaagaaagt gaagaagta cacgatcta cctgaagaa actgttcag gcaaaaagc gaagatgaa gttgaagag acagaaaat aacgaaata gaagaacct caagccgac ttagaagaa agtgaacct caattggac gagaaagaa gaagaaagt caggtcaaa agtgaagaa ctgacggta gaagaaaaa gaaattcaa gttgaagta gaaaaagtt tgcgccaaa tcggtagat gaacttgaa gcgccagaa aaaaaagaa gaagttgaa gaaaaaata gaaaaagag aagtctcca ctggccgtc agaaacatc caaagaata ggagaccgc ggtcgaaga cagccaaaa actggctgg ctttaa
- >TC007743 RA
gcctaaatt tgtgcaaaa tgggggatt cggttgatg ttgaggcta aagtcacac gcaagaccc taatcatgc ccaacttgc ccaagtcac tttcaattg aagaaaaaa aatatttat tggccattg aacgtggag acatggcaa atgttcgcc gaatgttgc aaaaagccc accggaaaa aatcgtttg atattaatt gtgtggata gtctaggac gtggtgctt tatcgattg ccatcgacc aagagcatc tcgaaatgg tggaactcc tggtgatca tgggagtgg aaactagag atgctcttc tgcatgcta ttcacgtag agtttgtgg aagctgttg agcttttgt tggaacacg aggagttga tccacaaag atggggagc cttatagtt ggcaaaaag ttgacatca acactgcca tgttcacac ctgatatca ctcccttga ctttggccg cccatcgaa ataattacg aaattttga aaattttgt tagacagag gagcgacta ttccgatgc cacatgata tcaaatgtg gatgtgatg aatgtatca aagaatctg aagaggatt atttacgac actcgttag cgagactaa acgagtaca aagctctgg caagtccgt ctttgattg cactttcgt caagtgatc cgattttaa cagcgtttc aattgtcgt gggaattga ggaatctgg cgtttgctg aaccggaat gtaaatccg aatacatgg acctgcgcc gccaatgcc aacaattcg ctgtcgacc tcctccacc aaactcgca gctcccaag aactagcgt tcatcctca accacgacc cagccgccc caccttacg aagaaggcg aacacatga aacttgcac gcctcgaac tcgccattc aatacaaac aaaaaaaat tcgtagccc atccaaaca tccaacaac tcttagccg ccatctggt acgaaggcg ttcccggat ttcggcgca aatcctcaa cccaaaaaa tcatgatca ttgtcaaag tggccgttt tattcccct tctattgca tgctataca tgataacac ccggaacga aaaccggga aactaatga gaaaaccct tcatgaaat tcttaattc atgcctcgt cctatttgt tctttttgt ttttgttaa tcctggtgt ctcaacgag ccgaagacc aaatcatcg aattgttcg gaaccgaaa gcatgaaac aaaacctac aggaaaagt accgaaaac aaaggggaa accccccaa ctatcctag aatatgttg tcttgattt atgttattg ggttcattt tcgaggaaa ctcacgaga tttatgtgg aaggaatga aatcgtatt tgcgtaact tgtggaatt ttattgatt ttacgcgaa attttcttt atacggccg tttttttgt tacgtgtgg ctgcctata tccagcaaa cttccgaaa ttgaaaatg accctttta cggcatata tcaggcgag aggagtggc aagctttcg atccgcaat tgattgctg aggggcttt tcgcagccg caaatattt tcagtgcct taaaactgg tccatttgt tctccatca acccacatt tggggccgt tgcaaatat cgcttggaa gaatggtca tagacattg taaaattct tctttattt acactctgg tgctgtttg cttttgcct gcggtttga accaactct tgtggtact tttcggatt tggaaaaga agaaatgtt accatttac caagtggtg aagccgatt ttgataatg cggccgatt cgtgcatga agtggcgcc gtttcgcta acgtttttg agtcgtccc agtcgcttt tctgggcca gcttcggta tggtggacc tcgccagtt ttgagttaa ccggaatca aaacttaca cgagatttt ggggccttt taatgttcg gttcttact cggtgatca atgtcatcg ttttattga accttctaa tagccatga tgtcaaact cgtatgcaa tgatcgacg aacattcag acaccgaat ggaaatttg cacgaacca aactatgga tgagctatt tcgaagaaa gcgctactt taccgtcac ccttcaaca tcttcccca aacccaaac acttgttca aacttttgg gcttgcgca agaaagaca aaatacgaa aaatgtcaa cgaagcgaa gaaatcgcg aggaaaaag aacgcgatt acaaatata ctgccgtta tgcgggctt tagtttggc gttatgtct cagcaatgc accgaaagc tggaagaaa acgctgtga ctgaagacg atgttaatg aactaaaga gcgacattt cgtcgtttc gttatgaat tattggagg tgttgcaga aaaatggca tggatattt cttcggctg aaatgaagg aaaaggccg ttttgggcc gaaaaatga aagtttggg aacgaagac tgatgaagg actttaaag ttgcgccgg ttgttacgg aagaagagg aagaattca tctaccaac cccctccag aaggcgaaa actccctag ccaagtttc ggcgaatcg cgagattag ctgttgtta actcaaatt tgagtaaat ggaggcagg tggtcaaag gtgcctgca ttgcgtcgc agattggcc actgccaca gtcgcgact cgttcaaaa agcagcaaa atcttcaaa gagcgatgg aagaagcgc ggaaattga aagtgaaaa gtccggatc agagtcgcg ccacgactc cgatccaac ttccggaca ccaccggat ccaacatca ttaaaataa tccggggaa tcacccccg aaaccgtcg atacgaaat attgcctaa ctcccagag acaagagtc ctttgattt ccatttctc caccgaaaa cgccggaaa aagaaccgg atttgatca acttagaga gtgccaaga cctcgcccg ttctcccag tcaaggcta tgccctctg atgatgtgt cgttggaat cggttgata aaattatca acgactcca attctctag atgatgata aagttagaa gtttgccaa cttcaccaa cttcgagtg acgataaaa gtttggata gtttgagca aaaaagcac ttttctcat cgggaagtt tcactcccg aagaaaatg agaagaaag aagaagaaa ctgagccga aaaatgtca cttttagtt ttgatttta tcgatgaga gtagtaaag gggagagtt cagccgaaa tattgaaga aatcagagg aagaaactg aagaaaatc tggacaaaa gtgatgaac cggaaccat cacttgaag aaactaagg aagaaagtg aagaagtac acgatctac ctgaagaaa ctgttcagg caaaaagcg aagatgaag ttgaagaga cagaaaata acgaaatag aagaacctc aagccgact tagaagaaa gtgaacctc aattggacg agaaagaag aagaaagtc aggtcaaaa gtgaagaac tgacggtag aagaaaaag aaattcaag ttgaagtag aaaaagttt gcgccaaat cggtagatg aacttgaag cgccagaaa aaaaagaag aagttgaag aaaaaatag aaaaagaga agtctccac tggccgtca gaaacatcc aaagaatag gagaccgcg gtcgaagac agccaaaaa ctggctggc tttaattat attaatcat ttgtaccca ttttcaagt ttatcaaac atttattat gtaaaatgt tgaaggaat aataaaatt ttgaacgcc tt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
