Status
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Gene information
General information
TC007743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 483254..488677
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007743
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: sometimes insideout
    head partially remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007743 RA
      MGDSVDVEA KVTRKTLIM PNLPKSLSI EEKKYLLAI 
ERGDMANVR RMLQKAHRK KSFDINCVD SLGRGALSI 
AIDQEHLEM VELLVIMGV ETRDALLHA IHVEFVEAV 
ELLLEHEEL IHKDGEPYS WQKVDINTA MFTPDITPL 
TLAAHRNNY EILKILLDR GATIPMPHD IKCGCDECI 
KESEEDYLR HSLARLNEY KALASPSLI ALSSSDPIL 
TAFQLSWEL RNLAFAEPE CKSEYMDLR RQCQQFAVD 
LLHQTRSSQ ELAFILNHD PAAPPYEEG EHMKLARLE 
LAIQYKQKK FVAHPNIQQ LLAAIWYEG VPGFRRKSS 
TQKIMIIVK VAVLFPFYC MLYMITPGT KTGKLMRKP 
FMKFLIHAS SYLFFLFLL ILVSQRAED QIIELFGTE 
SMKQNLQEK YRKQRGNPP TILEYVVLI YVIGFIFEE 
THEIYVEGM KSYLRNLWN FIDFTRNFL YTAVFLLRV 
AAYIQQTSE IENDPFTAY IRREEWQAF DPQLIAEGL 
FAAANIFSA LKLVHLFSI NPHLGPLQI SLGRMVIDI 
VKFFFIYTL VLFAFACGL NQLLWYFSD LEKKKCYHL 
PSGEADFDN AADSCMKWR RFANVFESS QSLFWASFG 
MVDLASFEL TGIKTYTRF WGLLMFGSY SVINVIVLL 
NLLIAMMSN SYAMIDEHS DTEWKFART KLWMSYFEE 
SATLPSPFN IFPKPKHLF KLLGLRKKD KIRKMSTKR 
RNREEKERD YKYTAVMRA LVWRYVSAM HRKLEENAV 
TEDDVNELK SDISSFRYE LLEVLQKNG MDISSAEMK 
EKAVLGRKM KVWERRLMK DFKVAPVVT EEEEEFIYQ 
PPPEGENSL AKFRRIARL AVVNSNLSK WRQVVKGAC 
IASQIGHCH SRDSFKKQQ NLQRAMEEA RKLKVKSPD 
QSRATTPIQ LPDTTGSNI IKIIRGITP ETVDTKYCL 
TPRDKSPLI SISPPKTPE KEPDLINLE SAKTSPVLP 
VKAMPSDDV SLESVDKII NDSNSLDDD KVRSLPTSP 
TSSDDKSLD SLSKKALFS SGSFTPEEN EKKEEETEP 
KNVTFSFDF IDESSKGES SAEILKKSE EETEENLDK 
SDEPEPSLE ETKEESEEV HDLPEETVQ AKSEDEVEE 
TENNEIEEP QADLEESEP QLDEKEEES QVKSEELTV 
EEKEIQVEV EKVCAKSVD ELEAPEKKE EVEEKIEKE 
KSPLAVRNI QRIGDRGRR QPKTGWL
    CDS sequence
    • >TC007743 RA
      atgggggat tcggttgat gttgaggct aaagtcaca 
cgcaagacc ctaatcatg cccaacttg cccaagtca 
ctttcaatt gaagaaaaa aaatattta ttggccatt 
gaacgtgga gacatggca aatgttcgc cgaatgttg 
caaaaagcc caccggaaa aaatcgttt gatattaat 
tgtgtggat agtctagga cgtggtgct ttatcgatt 
gccatcgac caagagcat ctcgaaatg gtggaactc 
ctggtgatc atgggagtg gaaactaga gatgctctt 
ctgcatgct attcacgta gagtttgtg gaagctgtt 
gagcttttg ttggaacac gaggagttg atccacaaa 
gatggggag ccttatagt tggcaaaaa gttgacatc 
aacactgcc atgttcaca cctgatatc actcccttg 
actttggcc gcccatcga aataattac gaaattttg 
aaaattttg ttagacaga ggagcgact attccgatg 
ccacatgat atcaaatgt ggatgtgat gaatgtatc 
aaagaatct gaagaggat tatttacga cactcgtta 
gcgagacta aacgagtac aaagctctg gcaagtccg 
tctttgatt gcactttcg tcaagtgat ccgatttta 
acagcgttt caattgtcg tgggaattg aggaatctg 
gcgtttgct gaaccggaa tgtaaatcc gaatacatg 
gacctgcgc cgccaatgc caacaattc gctgtcgac 
ctcctccac caaactcgc agctcccaa gaactagcg 
ttcatcctc aaccacgac ccagccgcc ccaccttac 
gaagaaggc gaacacatg aaacttgca cgcctcgaa 
ctcgccatt caatacaaa caaaaaaaa ttcgtagcc 
catccaaac atccaacaa ctcttagcc gccatctgg 
tacgaaggc gttcccgga tttcggcgc aaatcctca 
acccaaaaa atcatgatc attgtcaaa gtggccgtt 
ttattcccc ttctattgc atgctatac atgataaca 
cccggaacg aaaaccggg aaactaatg agaaaaccc 
ttcatgaaa ttcttaatt catgcctcg tcctatttg 
ttctttttg tttttgtta atcctggtg tctcaacga 
gccgaagac caaatcatc gaattgttc ggaaccgaa 
agcatgaaa caaaaccta caggaaaag taccgaaaa 
caaagggga aacccccca actatccta gaatatgtt 
gtcttgatt tatgttatt gggttcatt ttcgaggaa 
actcacgag atttatgtg gaaggaatg aaatcgtat 
ttgcgtaac ttgtggaat tttattgat tttacgcga 
aattttctt tatacggcc gtttttttg ttacgtgtg 
gctgcctat atccagcaa acttccgaa attgaaaat 
gaccctttt acggcatat atcaggcga gaggagtgg 
caagctttc gatccgcaa ttgattgct gaggggctt 
ttcgcagcc gcaaatatt ttcagtgcc ttaaaactg 
gtccatttg ttctccatc aacccacat ttggggccg 
ttgcaaata tcgcttgga agaatggtc atagacatt 
gtaaaattc ttctttatt tacactctg gtgctgttt 
gcttttgcc tgcggtttg aaccaactc ttgtggtac 
ttttcggat ttggaaaag aagaaatgt taccattta 
ccaagtggt gaagccgat tttgataat gcggccgat 
tcgtgcatg aagtggcgc cgtttcgct aacgttttt 
gagtcgtcc cagtcgctt ttctgggcc agcttcggt 
atggtggac ctcgccagt tttgagtta accggaatc 
aaaacttac acgagattt tggggcctt ttaatgttc 
ggttcttac tcggtgatc aatgtcatc gttttattg 
aaccttcta atagccatg atgtcaaac tcgtatgca 
atgatcgac gaacattca gacaccgaa tggaaattt 
gcacgaacc aaactatgg atgagctat ttcgaagaa 
agcgctact ttaccgtca cccttcaac atcttcccc 
aaacccaaa cacttgttc aaacttttg ggcttgcgc 
aagaaagac aaaatacga aaaatgtca acgaagcga 
agaaatcgc gaggaaaaa gaacgcgat tacaaatat 
actgccgtt atgcgggct ttagtttgg cgttatgtc 
tcagcaatg caccgaaag ctggaagaa aacgctgtg 
actgaagac gatgttaat gaactaaag agcgacatt 
tcgtcgttt cgttatgaa ttattggag gtgttgcag 
aaaaatggc atggatatt tcttcggct gaaatgaag 
gaaaaggcc gttttgggc cgaaaaatg aaagtttgg 
gaacgaaga ctgatgaag gactttaaa gttgcgccg 
gttgttacg gaagaagag gaagaattc atctaccaa 
ccccctcca gaaggcgaa aactcccta gccaagttt 
cggcgaatc gcgagatta gctgttgtt aactcaaat 
ttgagtaaa tggaggcag gtggtcaaa ggtgcctgc 
attgcgtcg cagattggc cactgccac agtcgcgac 
tcgttcaaa aagcagcaa aatcttcaa agagcgatg 
gaagaagcg cggaaattg aaagtgaaa agtccggat 
cagagtcgc gccacgact ccgatccaa cttccggac 
accaccgga tccaacatc attaaaata atccgggga 
atcaccccc gaaaccgtc gatacgaaa tattgccta 
actcccaga gacaagagt cctttgatt tccatttct 
ccaccgaaa acgccggaa aaagaaccg gatttgatc 
aacttagag agtgccaag acctcgccc gttctccca 
gtcaaggct atgccctct gatgatgtg tcgttggaa 
tcggttgat aaaattatc aacgactcc aattctcta 
gatgatgat aaagttaga agtttgcca acttcacca 
acttcgagt gacgataaa agtttggat agtttgagc 
aaaaaagca cttttctca tcgggaagt ttcactccc 
gaagaaaat gagaagaaa gaagaagaa actgagccg 
aaaaatgtc acttttagt tttgatttt atcgatgag 
agtagtaaa ggggagagt tcagccgaa atattgaag 
aaatcagag gaagaaact gaagaaaat ctggacaaa 
agtgatgaa ccggaacca tcacttgaa gaaactaag 
gaagaaagt gaagaagta cacgatcta cctgaagaa 
actgttcag gcaaaaagc gaagatgaa gttgaagag 
acagaaaat aacgaaata gaagaacct caagccgac 
ttagaagaa agtgaacct caattggac gagaaagaa 
gaagaaagt caggtcaaa agtgaagaa ctgacggta 
gaagaaaaa gaaattcaa gttgaagta gaaaaagtt 
tgcgccaaa tcggtagat gaacttgaa gcgccagaa 
aaaaaagaa gaagttgaa gaaaaaata gaaaaagag 
aagtctcca ctggccgtc agaaacatc caaagaata 
ggagaccgc ggtcgaaga cagccaaaa actggctgg 
ctttaa
    mRNA sequence
    • >TC007743 RA
      gcctaaatt tgtgcaaaa tgggggatt cggttgatg 
ttgaggcta aagtcacac gcaagaccc taatcatgc 
ccaacttgc ccaagtcac tttcaattg aagaaaaaa 
aatatttat tggccattg aacgtggag acatggcaa 
atgttcgcc gaatgttgc aaaaagccc accggaaaa 
aatcgtttg atattaatt gtgtggata gtctaggac 
gtggtgctt tatcgattg ccatcgacc aagagcatc 
tcgaaatgg tggaactcc tggtgatca tgggagtgg 
aaactagag atgctcttc tgcatgcta ttcacgtag 
agtttgtgg aagctgttg agcttttgt tggaacacg 
aggagttga tccacaaag atggggagc cttatagtt 
ggcaaaaag ttgacatca acactgcca tgttcacac 
ctgatatca ctcccttga ctttggccg cccatcgaa 
ataattacg aaattttga aaattttgt tagacagag 
gagcgacta ttccgatgc cacatgata tcaaatgtg 
gatgtgatg aatgtatca aagaatctg aagaggatt 
atttacgac actcgttag cgagactaa acgagtaca 
aagctctgg caagtccgt ctttgattg cactttcgt 
caagtgatc cgattttaa cagcgtttc aattgtcgt 
gggaattga ggaatctgg cgtttgctg aaccggaat 
gtaaatccg aatacatgg acctgcgcc gccaatgcc 
aacaattcg ctgtcgacc tcctccacc aaactcgca 
gctcccaag aactagcgt tcatcctca accacgacc 
cagccgccc caccttacg aagaaggcg aacacatga 
aacttgcac gcctcgaac tcgccattc aatacaaac 
aaaaaaaat tcgtagccc atccaaaca tccaacaac 
tcttagccg ccatctggt acgaaggcg ttcccggat 
ttcggcgca aatcctcaa cccaaaaaa tcatgatca 
ttgtcaaag tggccgttt tattcccct tctattgca 
tgctataca tgataacac ccggaacga aaaccggga 
aactaatga gaaaaccct tcatgaaat tcttaattc 
atgcctcgt cctatttgt tctttttgt ttttgttaa 
tcctggtgt ctcaacgag ccgaagacc aaatcatcg 
aattgttcg gaaccgaaa gcatgaaac aaaacctac 
aggaaaagt accgaaaac aaaggggaa accccccaa 
ctatcctag aatatgttg tcttgattt atgttattg 
ggttcattt tcgaggaaa ctcacgaga tttatgtgg 
aaggaatga aatcgtatt tgcgtaact tgtggaatt 
ttattgatt ttacgcgaa attttcttt atacggccg 
tttttttgt tacgtgtgg ctgcctata tccagcaaa 
cttccgaaa ttgaaaatg accctttta cggcatata 
tcaggcgag aggagtggc aagctttcg atccgcaat 
tgattgctg aggggcttt tcgcagccg caaatattt 
tcagtgcct taaaactgg tccatttgt tctccatca 
acccacatt tggggccgt tgcaaatat cgcttggaa 
gaatggtca tagacattg taaaattct tctttattt 
acactctgg tgctgtttg cttttgcct gcggtttga 
accaactct tgtggtact tttcggatt tggaaaaga 
agaaatgtt accatttac caagtggtg aagccgatt 
ttgataatg cggccgatt cgtgcatga agtggcgcc 
gtttcgcta acgtttttg agtcgtccc agtcgcttt 
tctgggcca gcttcggta tggtggacc tcgccagtt 
ttgagttaa ccggaatca aaacttaca cgagatttt 
ggggccttt taatgttcg gttcttact cggtgatca 
atgtcatcg ttttattga accttctaa tagccatga 
tgtcaaact cgtatgcaa tgatcgacg aacattcag 
acaccgaat ggaaatttg cacgaacca aactatgga 
tgagctatt tcgaagaaa gcgctactt taccgtcac 
ccttcaaca tcttcccca aacccaaac acttgttca 
aacttttgg gcttgcgca agaaagaca aaatacgaa 
aaatgtcaa cgaagcgaa gaaatcgcg aggaaaaag 
aacgcgatt acaaatata ctgccgtta tgcgggctt 
tagtttggc gttatgtct cagcaatgc accgaaagc 
tggaagaaa acgctgtga ctgaagacg atgttaatg 
aactaaaga gcgacattt cgtcgtttc gttatgaat 
tattggagg tgttgcaga aaaatggca tggatattt 
cttcggctg aaatgaagg aaaaggccg ttttgggcc 
gaaaaatga aagtttggg aacgaagac tgatgaagg 
actttaaag ttgcgccgg ttgttacgg aagaagagg 
aagaattca tctaccaac cccctccag aaggcgaaa 
actccctag ccaagtttc ggcgaatcg cgagattag 
ctgttgtta actcaaatt tgagtaaat ggaggcagg 
tggtcaaag gtgcctgca ttgcgtcgc agattggcc 
actgccaca gtcgcgact cgttcaaaa agcagcaaa 
atcttcaaa gagcgatgg aagaagcgc ggaaattga 
aagtgaaaa gtccggatc agagtcgcg ccacgactc 
cgatccaac ttccggaca ccaccggat ccaacatca 
ttaaaataa tccggggaa tcacccccg aaaccgtcg 
atacgaaat attgcctaa ctcccagag acaagagtc 
ctttgattt ccatttctc caccgaaaa cgccggaaa 
aagaaccgg atttgatca acttagaga gtgccaaga 
cctcgcccg ttctcccag tcaaggcta tgccctctg 
atgatgtgt cgttggaat cggttgata aaattatca 
acgactcca attctctag atgatgata aagttagaa 
gtttgccaa cttcaccaa cttcgagtg acgataaaa 
gtttggata gtttgagca aaaaagcac ttttctcat 
cgggaagtt tcactcccg aagaaaatg agaagaaag 
aagaagaaa ctgagccga aaaatgtca cttttagtt 
ttgatttta tcgatgaga gtagtaaag gggagagtt 
cagccgaaa tattgaaga aatcagagg aagaaactg 
aagaaaatc tggacaaaa gtgatgaac cggaaccat 
cacttgaag aaactaagg aagaaagtg aagaagtac 
acgatctac ctgaagaaa ctgttcagg caaaaagcg 
aagatgaag ttgaagaga cagaaaata acgaaatag 
aagaacctc aagccgact tagaagaaa gtgaacctc 
aattggacg agaaagaag aagaaagtc aggtcaaaa 
gtgaagaac tgacggtag aagaaaaag aaattcaag 
ttgaagtag aaaaagttt gcgccaaat cggtagatg 
aacttgaag cgccagaaa aaaaagaag aagttgaag 
aaaaaatag aaaaagaga agtctccac tggccgtca 
gaaacatcc aaagaatag gagaccgcg gtcgaagac 
agccaaaaa ctggctggc tttaattat attaatcat 
ttgtaccca ttttcaagt ttatcaaac atttattat 
gtaaaatgt tgaaggaat aataaaatt ttgaacgcc 
tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY