Status
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Gene information
General information
TC007743 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 483254..488677
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007743
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04376

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: sometimes insideout
    head partially remaining - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007743 RA
      MGDSVDVEA KVTRKTLIM PNLPKSLSI EEKKYLLAI 
      ERGDMANVR RMLQKAHRK KSFDINCVD SLGRGALSI 
      AIDQEHLEM VELLVIMGV ETRDALLHA IHVEFVEAV 
      ELLLEHEEL IHKDGEPYS WQKVDINTA MFTPDITPL 
      TLAAHRNNY EILKILLDR GATIPMPHD IKCGCDECI 
      KESEEDYLR HSLARLNEY KALASPSLI ALSSSDPIL 
      TAFQLSWEL RNLAFAEPE CKSEYMDLR RQCQQFAVD 
      LLHQTRSSQ ELAFILNHD PAAPPYEEG EHMKLARLE 
      LAIQYKQKK FVAHPNIQQ LLAAIWYEG VPGFRRKSS 
      TQKIMIIVK VAVLFPFYC MLYMITPGT KTGKLMRKP 
      FMKFLIHAS SYLFFLFLL ILVSQRAED QIIELFGTE 
      SMKQNLQEK YRKQRGNPP TILEYVVLI YVIGFIFEE 
      THEIYVEGM KSYLRNLWN FIDFTRNFL YTAVFLLRV 
      AAYIQQTSE IENDPFTAY IRREEWQAF DPQLIAEGL 
      FAAANIFSA LKLVHLFSI NPHLGPLQI SLGRMVIDI 
      VKFFFIYTL VLFAFACGL NQLLWYFSD LEKKKCYHL 
      PSGEADFDN AADSCMKWR RFANVFESS QSLFWASFG 
      MVDLASFEL TGIKTYTRF WGLLMFGSY SVINVIVLL 
      NLLIAMMSN SYAMIDEHS DTEWKFART KLWMSYFEE 
      SATLPSPFN IFPKPKHLF KLLGLRKKD KIRKMSTKR 
      RNREEKERD YKYTAVMRA LVWRYVSAM HRKLEENAV 
      TEDDVNELK SDISSFRYE LLEVLQKNG MDISSAEMK 
      EKAVLGRKM KVWERRLMK DFKVAPVVT EEEEEFIYQ 
      PPPEGENSL AKFRRIARL AVVNSNLSK WRQVVKGAC 
      IASQIGHCH SRDSFKKQQ NLQRAMEEA RKLKVKSPD 
      QSRATTPIQ LPDTTGSNI IKIIRGITP ETVDTKYCL 
      TPRDKSPLI SISPPKTPE KEPDLINLE SAKTSPVLP 
      VKAMPSDDV SLESVDKII NDSNSLDDD KVRSLPTSP 
      TSSDDKSLD SLSKKALFS SGSFTPEEN EKKEEETEP 
      KNVTFSFDF IDESSKGES SAEILKKSE EETEENLDK 
      SDEPEPSLE ETKEESEEV HDLPEETVQ AKSEDEVEE 
      TENNEIEEP QADLEESEP QLDEKEEES QVKSEELTV 
      EEKEIQVEV EKVCAKSVD ELEAPEKKE EVEEKIEKE 
      KSPLAVRNI QRIGDRGRR QPKTGWL
    CDS sequence
    • >TC007743 RA
      atgggggat tcggttgat gttgaggct aaagtcaca 
      cgcaagacc ctaatcatg cccaacttg cccaagtca 
      ctttcaatt gaagaaaaa aaatattta ttggccatt 
      gaacgtgga gacatggca aatgttcgc cgaatgttg 
      caaaaagcc caccggaaa aaatcgttt gatattaat 
      tgtgtggat agtctagga cgtggtgct ttatcgatt 
      gccatcgac caagagcat ctcgaaatg gtggaactc 
      ctggtgatc atgggagtg gaaactaga gatgctctt 
      ctgcatgct attcacgta gagtttgtg gaagctgtt 
      gagcttttg ttggaacac gaggagttg atccacaaa 
      gatggggag ccttatagt tggcaaaaa gttgacatc 
      aacactgcc atgttcaca cctgatatc actcccttg 
      actttggcc gcccatcga aataattac gaaattttg 
      aaaattttg ttagacaga ggagcgact attccgatg 
      ccacatgat atcaaatgt ggatgtgat gaatgtatc 
      aaagaatct gaagaggat tatttacga cactcgtta 
      gcgagacta aacgagtac aaagctctg gcaagtccg 
      tctttgatt gcactttcg tcaagtgat ccgatttta 
      acagcgttt caattgtcg tgggaattg aggaatctg 
      gcgtttgct gaaccggaa tgtaaatcc gaatacatg 
      gacctgcgc cgccaatgc caacaattc gctgtcgac 
      ctcctccac caaactcgc agctcccaa gaactagcg 
      ttcatcctc aaccacgac ccagccgcc ccaccttac 
      gaagaaggc gaacacatg aaacttgca cgcctcgaa 
      ctcgccatt caatacaaa caaaaaaaa ttcgtagcc 
      catccaaac atccaacaa ctcttagcc gccatctgg 
      tacgaaggc gttcccgga tttcggcgc aaatcctca 
      acccaaaaa atcatgatc attgtcaaa gtggccgtt 
      ttattcccc ttctattgc atgctatac atgataaca 
      cccggaacg aaaaccggg aaactaatg agaaaaccc 
      ttcatgaaa ttcttaatt catgcctcg tcctatttg 
      ttctttttg tttttgtta atcctggtg tctcaacga 
      gccgaagac caaatcatc gaattgttc ggaaccgaa 
      agcatgaaa caaaaccta caggaaaag taccgaaaa 
      caaagggga aacccccca actatccta gaatatgtt 
      gtcttgatt tatgttatt gggttcatt ttcgaggaa 
      actcacgag atttatgtg gaaggaatg aaatcgtat 
      ttgcgtaac ttgtggaat tttattgat tttacgcga 
      aattttctt tatacggcc gtttttttg ttacgtgtg 
      gctgcctat atccagcaa acttccgaa attgaaaat 
      gaccctttt acggcatat atcaggcga gaggagtgg 
      caagctttc gatccgcaa ttgattgct gaggggctt 
      ttcgcagcc gcaaatatt ttcagtgcc ttaaaactg 
      gtccatttg ttctccatc aacccacat ttggggccg 
      ttgcaaata tcgcttgga agaatggtc atagacatt 
      gtaaaattc ttctttatt tacactctg gtgctgttt 
      gcttttgcc tgcggtttg aaccaactc ttgtggtac 
      ttttcggat ttggaaaag aagaaatgt taccattta 
      ccaagtggt gaagccgat tttgataat gcggccgat 
      tcgtgcatg aagtggcgc cgtttcgct aacgttttt 
      gagtcgtcc cagtcgctt ttctgggcc agcttcggt 
      atggtggac ctcgccagt tttgagtta accggaatc 
      aaaacttac acgagattt tggggcctt ttaatgttc 
      ggttcttac tcggtgatc aatgtcatc gttttattg 
      aaccttcta atagccatg atgtcaaac tcgtatgca 
      atgatcgac gaacattca gacaccgaa tggaaattt 
      gcacgaacc aaactatgg atgagctat ttcgaagaa 
      agcgctact ttaccgtca cccttcaac atcttcccc 
      aaacccaaa cacttgttc aaacttttg ggcttgcgc 
      aagaaagac aaaatacga aaaatgtca acgaagcga 
      agaaatcgc gaggaaaaa gaacgcgat tacaaatat 
      actgccgtt atgcgggct ttagtttgg cgttatgtc 
      tcagcaatg caccgaaag ctggaagaa aacgctgtg 
      actgaagac gatgttaat gaactaaag agcgacatt 
      tcgtcgttt cgttatgaa ttattggag gtgttgcag 
      aaaaatggc atggatatt tcttcggct gaaatgaag 
      gaaaaggcc gttttgggc cgaaaaatg aaagtttgg 
      gaacgaaga ctgatgaag gactttaaa gttgcgccg 
      gttgttacg gaagaagag gaagaattc atctaccaa 
      ccccctcca gaaggcgaa aactcccta gccaagttt 
      cggcgaatc gcgagatta gctgttgtt aactcaaat 
      ttgagtaaa tggaggcag gtggtcaaa ggtgcctgc 
      attgcgtcg cagattggc cactgccac agtcgcgac 
      tcgttcaaa aagcagcaa aatcttcaa agagcgatg 
      gaagaagcg cggaaattg aaagtgaaa agtccggat 
      cagagtcgc gccacgact ccgatccaa cttccggac 
      accaccgga tccaacatc attaaaata atccgggga 
      atcaccccc gaaaccgtc gatacgaaa tattgccta 
      actcccaga gacaagagt cctttgatt tccatttct 
      ccaccgaaa acgccggaa aaagaaccg gatttgatc 
      aacttagag agtgccaag acctcgccc gttctccca 
      gtcaaggct atgccctct gatgatgtg tcgttggaa 
      tcggttgat aaaattatc aacgactcc aattctcta 
      gatgatgat aaagttaga agtttgcca acttcacca 
      acttcgagt gacgataaa agtttggat agtttgagc 
      aaaaaagca cttttctca tcgggaagt ttcactccc 
      gaagaaaat gagaagaaa gaagaagaa actgagccg 
      aaaaatgtc acttttagt tttgatttt atcgatgag 
      agtagtaaa ggggagagt tcagccgaa atattgaag 
      aaatcagag gaagaaact gaagaaaat ctggacaaa 
      agtgatgaa ccggaacca tcacttgaa gaaactaag 
      gaagaaagt gaagaagta cacgatcta cctgaagaa 
      actgttcag gcaaaaagc gaagatgaa gttgaagag 
      acagaaaat aacgaaata gaagaacct caagccgac 
      ttagaagaa agtgaacct caattggac gagaaagaa 
      gaagaaagt caggtcaaa agtgaagaa ctgacggta 
      gaagaaaaa gaaattcaa gttgaagta gaaaaagtt 
      tgcgccaaa tcggtagat gaacttgaa gcgccagaa 
      aaaaaagaa gaagttgaa gaaaaaata gaaaaagag 
      aagtctcca ctggccgtc agaaacatc caaagaata 
      ggagaccgc ggtcgaaga cagccaaaa actggctgg 
      ctttaa
    mRNA sequence
    • >TC007743 RA
      gcctaaatt tgtgcaaaa tgggggatt cggttgatg 
      ttgaggcta aagtcacac gcaagaccc taatcatgc 
      ccaacttgc ccaagtcac tttcaattg aagaaaaaa 
      aatatttat tggccattg aacgtggag acatggcaa 
      atgttcgcc gaatgttgc aaaaagccc accggaaaa 
      aatcgtttg atattaatt gtgtggata gtctaggac 
      gtggtgctt tatcgattg ccatcgacc aagagcatc 
      tcgaaatgg tggaactcc tggtgatca tgggagtgg 
      aaactagag atgctcttc tgcatgcta ttcacgtag 
      agtttgtgg aagctgttg agcttttgt tggaacacg 
      aggagttga tccacaaag atggggagc cttatagtt 
      ggcaaaaag ttgacatca acactgcca tgttcacac 
      ctgatatca ctcccttga ctttggccg cccatcgaa 
      ataattacg aaattttga aaattttgt tagacagag 
      gagcgacta ttccgatgc cacatgata tcaaatgtg 
      gatgtgatg aatgtatca aagaatctg aagaggatt 
      atttacgac actcgttag cgagactaa acgagtaca 
      aagctctgg caagtccgt ctttgattg cactttcgt 
      caagtgatc cgattttaa cagcgtttc aattgtcgt 
      gggaattga ggaatctgg cgtttgctg aaccggaat 
      gtaaatccg aatacatgg acctgcgcc gccaatgcc 
      aacaattcg ctgtcgacc tcctccacc aaactcgca 
      gctcccaag aactagcgt tcatcctca accacgacc 
      cagccgccc caccttacg aagaaggcg aacacatga 
      aacttgcac gcctcgaac tcgccattc aatacaaac 
      aaaaaaaat tcgtagccc atccaaaca tccaacaac 
      tcttagccg ccatctggt acgaaggcg ttcccggat 
      ttcggcgca aatcctcaa cccaaaaaa tcatgatca 
      ttgtcaaag tggccgttt tattcccct tctattgca 
      tgctataca tgataacac ccggaacga aaaccggga 
      aactaatga gaaaaccct tcatgaaat tcttaattc 
      atgcctcgt cctatttgt tctttttgt ttttgttaa 
      tcctggtgt ctcaacgag ccgaagacc aaatcatcg 
      aattgttcg gaaccgaaa gcatgaaac aaaacctac 
      aggaaaagt accgaaaac aaaggggaa accccccaa 
      ctatcctag aatatgttg tcttgattt atgttattg 
      ggttcattt tcgaggaaa ctcacgaga tttatgtgg 
      aaggaatga aatcgtatt tgcgtaact tgtggaatt 
      ttattgatt ttacgcgaa attttcttt atacggccg 
      tttttttgt tacgtgtgg ctgcctata tccagcaaa 
      cttccgaaa ttgaaaatg accctttta cggcatata 
      tcaggcgag aggagtggc aagctttcg atccgcaat 
      tgattgctg aggggcttt tcgcagccg caaatattt 
      tcagtgcct taaaactgg tccatttgt tctccatca 
      acccacatt tggggccgt tgcaaatat cgcttggaa 
      gaatggtca tagacattg taaaattct tctttattt 
      acactctgg tgctgtttg cttttgcct gcggtttga 
      accaactct tgtggtact tttcggatt tggaaaaga 
      agaaatgtt accatttac caagtggtg aagccgatt 
      ttgataatg cggccgatt cgtgcatga agtggcgcc 
      gtttcgcta acgtttttg agtcgtccc agtcgcttt 
      tctgggcca gcttcggta tggtggacc tcgccagtt 
      ttgagttaa ccggaatca aaacttaca cgagatttt 
      ggggccttt taatgttcg gttcttact cggtgatca 
      atgtcatcg ttttattga accttctaa tagccatga 
      tgtcaaact cgtatgcaa tgatcgacg aacattcag 
      acaccgaat ggaaatttg cacgaacca aactatgga 
      tgagctatt tcgaagaaa gcgctactt taccgtcac 
      ccttcaaca tcttcccca aacccaaac acttgttca 
      aacttttgg gcttgcgca agaaagaca aaatacgaa 
      aaatgtcaa cgaagcgaa gaaatcgcg aggaaaaag 
      aacgcgatt acaaatata ctgccgtta tgcgggctt 
      tagtttggc gttatgtct cagcaatgc accgaaagc 
      tggaagaaa acgctgtga ctgaagacg atgttaatg 
      aactaaaga gcgacattt cgtcgtttc gttatgaat 
      tattggagg tgttgcaga aaaatggca tggatattt 
      cttcggctg aaatgaagg aaaaggccg ttttgggcc 
      gaaaaatga aagtttggg aacgaagac tgatgaagg 
      actttaaag ttgcgccgg ttgttacgg aagaagagg 
      aagaattca tctaccaac cccctccag aaggcgaaa 
      actccctag ccaagtttc ggcgaatcg cgagattag 
      ctgttgtta actcaaatt tgagtaaat ggaggcagg 
      tggtcaaag gtgcctgca ttgcgtcgc agattggcc 
      actgccaca gtcgcgact cgttcaaaa agcagcaaa 
      atcttcaaa gagcgatgg aagaagcgc ggaaattga 
      aagtgaaaa gtccggatc agagtcgcg ccacgactc 
      cgatccaac ttccggaca ccaccggat ccaacatca 
      ttaaaataa tccggggaa tcacccccg aaaccgtcg 
      atacgaaat attgcctaa ctcccagag acaagagtc 
      ctttgattt ccatttctc caccgaaaa cgccggaaa 
      aagaaccgg atttgatca acttagaga gtgccaaga 
      cctcgcccg ttctcccag tcaaggcta tgccctctg 
      atgatgtgt cgttggaat cggttgata aaattatca 
      acgactcca attctctag atgatgata aagttagaa 
      gtttgccaa cttcaccaa cttcgagtg acgataaaa 
      gtttggata gtttgagca aaaaagcac ttttctcat 
      cgggaagtt tcactcccg aagaaaatg agaagaaag 
      aagaagaaa ctgagccga aaaatgtca cttttagtt 
      ttgatttta tcgatgaga gtagtaaag gggagagtt 
      cagccgaaa tattgaaga aatcagagg aagaaactg 
      aagaaaatc tggacaaaa gtgatgaac cggaaccat 
      cacttgaag aaactaagg aagaaagtg aagaagtac 
      acgatctac ctgaagaaa ctgttcagg caaaaagcg 
      aagatgaag ttgaagaga cagaaaata acgaaatag 
      aagaacctc aagccgact tagaagaaa gtgaacctc 
      aattggacg agaaagaag aagaaagtc aggtcaaaa 
      gtgaagaac tgacggtag aagaaaaag aaattcaag 
      ttgaagtag aaaaagttt gcgccaaat cggtagatg 
      aacttgaag cgccagaaa aaaaagaag aagttgaag 
      aaaaaatag aaaaagaga agtctccac tggccgtca 
      gaaacatcc aaagaatag gagaccgcg gtcgaagac 
      agccaaaaa ctggctggc tttaattat attaatcat 
      ttgtaccca ttttcaagt ttatcaaac atttattat 
      gtaaaatgt tgaaggaat aataaaatt ttgaacgcc 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY