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Gene information
General information
TC007732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 287699..288950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007732
    iBeetle screen iB_04374

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007732 RA
      MLSSPIKPN QTDDSNKEN ISDNPLNIG DTCRDTKTS 
SLPLEQEHE EYQYLSHIK LIIDKGVKK SDRTGVGTF 
SIFGAQMRY SLRDNVFPL LTTKRVFWR GVVEELLWF 
IKGSTNANE LKEKNVHIW DANSTREFL DSVGLNERE 
EGDLGPVYG FQWRHFGAE YKGMHWDYA NQGIDQLAH 
VIETIRNKP NDRRIIMCA WNPVDIPEM ALPPCHCLV 
QFFVANGEL SCLLYQRSA DMGLGVPFN IASYSLLTY 
MIAHVTGLK PGEFIHTLG DSHVYLNHV EPLQEQLKR 
TPRPFPTLK IKRQVSSIE DFVFDDFEL SGYEPHPKL 
SMPMAV
    CDS sequence
    • >TC007732 RA
      atgttatcc tctccaatc aaacctaac caaaccgac 
gactcaaac aaggaaaac atctctgat aacccctta 
aatatcgga gacacgtgt cgcgacacc aaaaccagt 
agtttgccc cttgagcaa gaacacgaa gagtaccaa 
tacctgagc cacattaaa ctaatcatc gataagggt 
gtaaaaaaa agtgaccgc accggtgtt ggcactttt 
tcgattttt ggagcccaa atgcgctac tccctccgc 
gacaatgtc ttccctctg ttgaccacg aaacgtgtg 
ttctggcgc ggagtcgtt gaggagttg ctttggttc 
atcaaaggc tcaacgaac gcaaacgaa ttaaaagag 
aaaaatgtg catatttgg gatgcgaac agcacacga 
gagtttctc gattctgtg gggctcaat gagcgcgag 
gagggcgat ctgggaccg gtttatggt ttccagtgg 
cgccacttt ggggccgag tacaagggg atgcactgg 
gattacgcc aatcaaggg attgaccaa ttggctcat 
gttatcgaa acaattcgt aataaaccc aacgatagg 
agaatcata atgtgtgcg tggaatcca gtggatatt 
cccgaaatg gccctcccg ccttgtcac tgcctcgtg 
cagtttttt gtggcaaat ggggaattg tcgtgtttg 
ttgtaccaa aggtcggct gatatgggg ctgggggtg 
ccgttcaat attgcaagt tattccttg ttgacgtat 
atgattgca catgttaca ggattgaag cccggggaa 
tttattcat acgttaggg gacagtcat gtctacttg 
aatcatgtc gaaccgttg caagaacaa ttaaagagg 
acaccaagg ccgtttcca acattaaaa atcaaaaga 
caagtttcc agtattgag gattttgtt tttgatgat 
tttgagttg agtgggtac gagccccat cccaaattg 
agtatgccg atggctgtt tga
    mRNA sequence
    • >TC007732 RA
      cgacgccaa ctagtccaa attggcggg aaagttccg 
catcaaatc ctaagttta gtgtgtgtt ttttctaaa 
tttccgtgt gtttagtgt tgttaaacc gtgaaaatg 
ttatcctct ccaatcaaa cctaaccaa accgacgac 
tcaaacaag gaaaacatc tctgataac cccttaaat 
atcggagac acgtgtcgc gacaccaaa accagtagt 
ttgcccctt gagcaagaa cacgaagag taccaatac 
ctgagccac attaaacta atcatcgat aagggtgta 
aaaaaaagt gaccgcacc ggtgttggc actttttcg 
atttttgga gcccaaatg cgctactcc ctccgcgac 
aatgtcttc cctctgttg accacgaaa cgtgtgttc 
tggcgcgga gtcgttgag gagttgctt tggttcatc 
aaaggctca acgaacgca aacgaatta aaagagaaa 
aatgtgcat atttgggat gcgaacagc acacgagag 
tttctcgat tctgtgggg ctcaatgag cgcgaggag 
ggcgatctg ggaccggtt tatggtttc cagtggcgc 
cactttggg gccgagtac aaggggatg cactgggat 
tacgccaat caagggatt gaccaattg gctcatgtt 
atcgaaaca attcgtaat aaacccaac gataggaga 
atcataatg tgtgcgtgg aatccagtg gatattccc 
gaaatggcc ctcccgcct tgtcactgc ctcgtgcag 
ttttttgtg gcaaatggg gaattgtcg tgtttgttg 
taccaaagg tcggctgat atggggctg ggggtgccg 
ttcaatatt gcaagttat tccttgttg acgtatatg 
attgcacat gttacagga ttgaagccc ggggaattt 
attcatacg ttaggggac agtcatgtc tacttgaat 
catgtcgaa ccgttgcaa gaacaatta aagaggaca 
ccaaggccg tttccaaca ttaaaaatc aaaagacaa 
gtttccagt attgaggat tttgttttt gatgatttt 
gagttgagt gggtacgag ccccatccc aaattgagt 
atgccgatg gctgtttga agtaaaaat atgtgttta 
atgtactta tctgtttat caaatatac gtcattttt 
ttat
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