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Gene information
General information
TC007732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 287699..288950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007732
    iBeetle screen iB_04374

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007732 RA
      MLSSPIKPN QTDDSNKEN ISDNPLNIG DTCRDTKTS 
      SLPLEQEHE EYQYLSHIK LIIDKGVKK SDRTGVGTF 
      SIFGAQMRY SLRDNVFPL LTTKRVFWR GVVEELLWF 
      IKGSTNANE LKEKNVHIW DANSTREFL DSVGLNERE 
      EGDLGPVYG FQWRHFGAE YKGMHWDYA NQGIDQLAH 
      VIETIRNKP NDRRIIMCA WNPVDIPEM ALPPCHCLV 
      QFFVANGEL SCLLYQRSA DMGLGVPFN IASYSLLTY 
      MIAHVTGLK PGEFIHTLG DSHVYLNHV EPLQEQLKR 
      TPRPFPTLK IKRQVSSIE DFVFDDFEL SGYEPHPKL 
      SMPMAV
    CDS sequence
    • >TC007732 RA
      atgttatcc tctccaatc aaacctaac caaaccgac 
      gactcaaac aaggaaaac atctctgat aacccctta 
      aatatcgga gacacgtgt cgcgacacc aaaaccagt 
      agtttgccc cttgagcaa gaacacgaa gagtaccaa 
      tacctgagc cacattaaa ctaatcatc gataagggt 
      gtaaaaaaa agtgaccgc accggtgtt ggcactttt 
      tcgattttt ggagcccaa atgcgctac tccctccgc 
      gacaatgtc ttccctctg ttgaccacg aaacgtgtg 
      ttctggcgc ggagtcgtt gaggagttg ctttggttc 
      atcaaaggc tcaacgaac gcaaacgaa ttaaaagag 
      aaaaatgtg catatttgg gatgcgaac agcacacga 
      gagtttctc gattctgtg gggctcaat gagcgcgag 
      gagggcgat ctgggaccg gtttatggt ttccagtgg 
      cgccacttt ggggccgag tacaagggg atgcactgg 
      gattacgcc aatcaaggg attgaccaa ttggctcat 
      gttatcgaa acaattcgt aataaaccc aacgatagg 
      agaatcata atgtgtgcg tggaatcca gtggatatt 
      cccgaaatg gccctcccg ccttgtcac tgcctcgtg 
      cagtttttt gtggcaaat ggggaattg tcgtgtttg 
      ttgtaccaa aggtcggct gatatgggg ctgggggtg 
      ccgttcaat attgcaagt tattccttg ttgacgtat 
      atgattgca catgttaca ggattgaag cccggggaa 
      tttattcat acgttaggg gacagtcat gtctacttg 
      aatcatgtc gaaccgttg caagaacaa ttaaagagg 
      acaccaagg ccgtttcca acattaaaa atcaaaaga 
      caagtttcc agtattgag gattttgtt tttgatgat 
      tttgagttg agtgggtac gagccccat cccaaattg 
      agtatgccg atggctgtt tga
    mRNA sequence
    • >TC007732 RA
      cgacgccaa ctagtccaa attggcggg aaagttccg 
      catcaaatc ctaagttta gtgtgtgtt ttttctaaa 
      tttccgtgt gtttagtgt tgttaaacc gtgaaaatg 
      ttatcctct ccaatcaaa cctaaccaa accgacgac 
      tcaaacaag gaaaacatc tctgataac cccttaaat 
      atcggagac acgtgtcgc gacaccaaa accagtagt 
      ttgcccctt gagcaagaa cacgaagag taccaatac 
      ctgagccac attaaacta atcatcgat aagggtgta 
      aaaaaaagt gaccgcacc ggtgttggc actttttcg 
      atttttgga gcccaaatg cgctactcc ctccgcgac 
      aatgtcttc cctctgttg accacgaaa cgtgtgttc 
      tggcgcgga gtcgttgag gagttgctt tggttcatc 
      aaaggctca acgaacgca aacgaatta aaagagaaa 
      aatgtgcat atttgggat gcgaacagc acacgagag 
      tttctcgat tctgtgggg ctcaatgag cgcgaggag 
      ggcgatctg ggaccggtt tatggtttc cagtggcgc 
      cactttggg gccgagtac aaggggatg cactgggat 
      tacgccaat caagggatt gaccaattg gctcatgtt 
      atcgaaaca attcgtaat aaacccaac gataggaga 
      atcataatg tgtgcgtgg aatccagtg gatattccc 
      gaaatggcc ctcccgcct tgtcactgc ctcgtgcag 
      ttttttgtg gcaaatggg gaattgtcg tgtttgttg 
      taccaaagg tcggctgat atggggctg ggggtgccg 
      ttcaatatt gcaagttat tccttgttg acgtatatg 
      attgcacat gttacagga ttgaagccc ggggaattt 
      attcatacg ttaggggac agtcatgtc tacttgaat 
      catgtcgaa ccgttgcaa gaacaatta aagaggaca 
      ccaaggccg tttccaaca ttaaaaatc aaaagacaa 
      gtttccagt attgaggat tttgttttt gatgatttt 
      gagttgagt gggtacgag ccccatccc aaattgagt 
      atgccgatg gctgtttga agtaaaaat atgtgttta 
      atgtactta tctgtttat caaatatac gtcattttt 
      ttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY