Status
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Gene information
General information
TC032719
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007728
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 275879..277858
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032719
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04373

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032719 RA
      MFLFIFLLL YDRISADIA LCPLDFYHE QFAERVEPE 
      REEPTTGLT IHDILPEDP KKYDKMRPP KKDGQPTKV 
      YFHVTVMGL DSIDEGSMT YAADIFFAQ TWKDHRLRL 
      PENMTSEYR LLEVDWLKN MWRPDSFFK NAKSVTFQT 
      MTIPNHYMW LYKDKTILY MVKLTLRLS CAMNFMIYP 
      HDTQECKLQ MESLSHTTD DMIFQWDPD VPLVVDENI 
      ELPQLALVH NHTADCTQV YSTGNFTCL EVVFELKRR 
      LGYYLFHTY IPTCLIVIM SWVSFWIKP EAAPARVTL 
      GVTSLLTLS TQHAKSQAS LPPVSYLKA VDAFMSVCT 
      IFVFMALME YCLVNIVLG DSDLPKPPQ PAKKPDSIF 
      DMAAKENSF LLTGAPKIP VVTPAQRAR NRAINIDRF 
      SRIFFPLLF TVLNGTYWI MFAEYI
    • >TC032719 RB
      MFLFIFLLL YDRISADIA TGLTIHDIL PEDPKKYDK 
      MRPPKKDGQ PTKVYFHVT VMGLDSIDE GSMTYAADI 
      FFAQTWKDH RLRLPENMT SEYRLLEVD WLKNMWRPD 
      SFFKNAKSV TFQTMTIPN HYMWLYKDK TILYMVKLT 
      LRLSCAMNF MIYPHDTQE CKLQMESLS HTTDDMIFQ 
      WDPDVPLVV DENIELPQL ALVHNHTAD CTQVYSTGN 
      FTCLEVVFE LKRRLGYYL FHTYIPTCL IVIMSWVSF 
      WIKPEAAPA RVTLGVTSL LTLSTQHAK SQASLPPVS 
      YLKAVDAFM SVCTIFVFM ALMEYCLVN IVLGDSDLP 
      KPPQPAKKP DSIFDMAAK ENSFLLTGA PKIPVVTPA 
      QRARNRAIN IDRFSRIFF PLLFTVLNG TYWIMFAEY 
      I
    CDS sequence
    • >TC032719 RA
      atgtttctc tttattttt cttcttctg tatgaccgt 
      attagtgcc gatattgcc ctctgtcct ctagatttt 
      taccacgag caatttgcg gaaagagtg gaacccgaa 
      cgtgaagaa ccgaccacc ggattgacc attcacgac 
      attctcccc gaagacccc aaaaagtac gataaaatg 
      cgcccgccg aaaaaagac ggacaaccc accaaagtc 
      tacttccat gtcactgtc atggggctt gatagtata 
      gacgagggg tccatgact tatgcggct gatattttt 
      ttcgcccag acttggaaa gaccaccgg ttgcggtta 
      ccggaaaat atgacctcg gagtataga ttattggag 
      gttgattgg ttgaaaaat atgtggagg cctgattcg 
      tttttcaaa aatgcgaaa agtgtgact tttcaaact 
      atgaccata ccaaaccac tacatgtgg ctgtataaa 
      gacaaaact attctctat atggttaaa cttacgtta 
      cgactttct tgcgccatg aatttcatg atttatccg 
      catgatacg caagagtgt aaactacag atggaaagt 
      ttgtcacac actaccgac gatatgatt tttcaatgg 
      gaccctgat gtcccattg gttgtggat gaaaacatc 
      gaattacca caacttgct ttagttcat aatcacaca 
      gctgattgt actcaagtc tattcaact ggaaatttc 
      acctgtctt gaagttgtt tttgagtta aagcgccgt 
      ctaggctac tatctcttc catacgtat attccgact 
      tgtctcatt gtaatcatg tcttgggtt tctttctgg 
      attaaacct gaggcagcc ccagctcga gtcacccta 
      ggagtcacc tctttgcta acactttcc actcaacat 
      gccaaaagt caagcatca cttcctcct gtttcttac 
      ctgaaagct gtggacgca tttatgtca gtctgcaca 
      atatttgtg tttatggcc cttatggaa tactgtttg 
      gtgaatata gtcctgggt gactcagat ttacccaaa 
      ccaccgcaa ccggccaaa aaaccagac tcaattttc 
      gacatggca gcaaaagag aattcgttt ttgttaacc 
      ggggctcca aaaatcccg gtggttaca ccggcacaa 
      agggctcgg aatagggcg attaatatt gataggttc 
      agtcggatt tttttccct ttgctcttc actgtcctg 
      aacgggacg tattggata atgttcgct gagtatatt 
      tag
    • >TC032719 RB
      atgtttctc tttattttt cttcttctg tatgaccgt 
      attagtgcc gatattgcc accggattg accattcac 
      gacattctc cccgaagac cccaaaaag tacgataaa 
      atgcgcccg ccgaaaaaa gacggacaa cccaccaaa 
      gtctacttc catgtcact gtcatgggg cttgatagt 
      atagacgag gggtccatg acttatgcg gctgatatt 
      tttttcgcc cagacttgg aaagaccac cggttgcgg 
      ttaccggaa aatatgacc tcggagtat agattattg 
      gaggttgat tggttgaaa aatatgtgg aggcctgat 
      tcgtttttc aaaaatgcg aaaagtgtg acttttcaa 
      actatgacc ataccaaac cactacatg tggctgtat 
      aaagacaaa actattctc tatatggtt aaacttacg 
      ttacgactt tcttgcgcc atgaatttc atgatttat 
      ccgcatgat acgcaagag tgtaaacta cagatggaa 
      agtttgtca cacactacc gacgatatg atttttcaa 
      tgggaccct gatgtccca ttggttgtg gatgaaaac 
      atcgaatta ccacaactt gctttagtt cataatcac 
      acagctgat tgtactcaa gtctattca actggaaat 
      ttcacctgt cttgaagtt gtttttgag ttaaagcgc 
      cgtctaggc tactatctc ttccatacg tatattccg 
      acttgtctc attgtaatc atgtcttgg gtttctttc 
      tggattaaa cctgaggca gccccagct cgagtcacc 
      ctaggagtc acctctttg ctaacactt tccactcaa 
      catgccaaa agtcaagca tcacttcct cctgtttct 
      tacctgaaa gctgtggac gcatttatg tcagtctgc 
      acaatattt gtgtttatg gcccttatg gaatactgt 
      ttggtgaat atagtcctg ggtgactca gatttaccc 
      aaaccaccg caaccggcc aaaaaacca gactcaatt 
      ttcgacatg gcagcaaaa gagaattcg tttttgtta 
      accggggct ccaaaaatc ccggtggtt acaccggca 
      caaagggct cggaatagg gcgattaat attgatagg 
      ttcagtcgg atttttttc cctttgctc ttcactgtc 
      ctgaacggg acgtattgg ataatgttc gctgagtat 
      atttag
    mRNA sequence
    • >TC032719 RA
      agtaccaag tgcataaga aggctttgc agcctcttc 
      agatttcag tcggcaatt cccagccgc cactaacat 
      gccattctt ataacaaaa aattcaaat attgataac 
      attgtggtg attatggat tgcggtaaa atgtttctc 
      tttattttt cttcttctg tatgaccgt attagtgcc 
      gatattgcc ctctgtcct ctagatttt taccacgag 
      caatttgcg gaaagagtg gaacccgaa cgtgaagaa 
      ccgaccacc ggattgacc attcacgac attctcccc 
      gaagacccc aaaaagtac gataaaatg cgcccgccg 
      aaaaaagac ggacaaccc accaaagtc tacttccat 
      gtcactgtc atggggctt gatagtata gacgagggg 
      tccatgact tatgcggct gatattttt ttcgcccag 
      acttggaaa gaccaccgg ttgcggtta ccggaaaat 
      atgacctcg gagtataga ttattggag gttgattgg 
      ttgaaaaat atgtggagg cctgattcg tttttcaaa 
      aatgcgaaa agtgtgact tttcaaact atgaccata 
      ccaaaccac tacatgtgg ctgtataaa gacaaaact 
      attctctat atggttaaa cttacgtta cgactttct 
      tgcgccatg aatttcatg atttatccg catgatacg 
      caagagtgt aaactacag atggaaagt ttgtcacac 
      actaccgac gatatgatt tttcaatgg gaccctgat 
      gtcccattg gttgtggat gaaaacatc gaattacca 
      caacttgct ttagttcat aatcacaca gctgattgt 
      actcaagtc tattcaact ggaaatttc acctgtctt 
      gaagttgtt tttgagtta aagcgccgt ctaggctac 
      tatctcttc catacgtat attccgact tgtctcatt 
      gtaatcatg tcttgggtt tctttctgg attaaacct 
      gaggcagcc ccagctcga gtcacccta ggagtcacc 
      tctttgcta acactttcc actcaacat gccaaaagt 
      caagcatca cttcctcct gtttcttac ctgaaagct 
      gtggacgca tttatgtca gtctgcaca atatttgtg 
      tttatggcc cttatggaa tactgtttg gtgaatata 
      gtcctgggt gactcagat ttacccaaa ccaccgcaa 
      ccggccaaa aaaccagac tcaattttc gacatggca 
      gcaaaagag aattcgttt ttgttaacc ggggctcca 
      aaaatcccg gtggttaca ccggcacaa agggctcgg 
      aatagggcg attaatatt gataggttc agtcggatt 
      tttttccct ttgctcttc actgtcctg aacgggacg 
      tattggata atgttcgct gagtatatt tagattatt 
      agttgagag ttaatgaat attacatta tgtagacac 
      tgattacgt taaatacat gctgagtca acaa
    • >TC032719 RB
      agtaccaag tgcataaga aggctttgc agcctcttc 
      agatttcag tcggcaatt cccagccgc cactaacat 
      gccattctt ataacaaaa aattcaaat attgataac 
      attgtggtg attatggat tgcggtaaa atgtttctc 
      tttattttt cttcttctg tatgaccgt attagtgcc 
      gatattgcc accggattg accattcac gacattctc 
      cccgaagac cccaaaaag tacgataaa atgcgcccg 
      ccgaaaaaa gacggacaa cccaccaaa gtctacttc 
      catgtcact gtcatgggg cttgatagt atagacgag 
      gggtccatg acttatgcg gctgatatt tttttcgcc 
      cagacttgg aaagaccac cggttgcgg ttaccggaa 
      aatatgacc tcggagtat agattattg gaggttgat 
      tggttgaaa aatatgtgg aggcctgat tcgtttttc 
      aaaaatgcg aaaagtgtg acttttcaa actatgacc 
      ataccaaac cactacatg tggctgtat aaagacaaa 
      actattctc tatatggtt aaacttacg ttacgactt 
      tcttgcgcc atgaatttc atgatttat ccgcatgat 
      acgcaagag tgtaaacta cagatggaa agtttgtca 
      cacactacc gacgatatg atttttcaa tgggaccct 
      gatgtccca ttggttgtg gatgaaaac atcgaatta 
      ccacaactt gctttagtt cataatcac acagctgat 
      tgtactcaa gtctattca actggaaat ttcacctgt 
      cttgaagtt gtttttgag ttaaagcgc cgtctaggc 
      tactatctc ttccatacg tatattccg acttgtctc 
      attgtaatc atgtcttgg gtttctttc tggattaaa 
      cctgaggca gccccagct cgagtcacc ctaggagtc 
      acctctttg ctaacactt tccactcaa catgccaaa 
      agtcaagca tcacttcct cctgtttct tacctgaaa 
      gctgtggac gcatttatg tcagtctgc acaatattt 
      gtgtttatg gcccttatg gaatactgt ttggtgaat 
      atagtcctg ggtgactca gatttaccc aaaccaccg 
      caaccggcc aaaaaacca gactcaatt ttcgacatg 
      gcagcaaaa gagaattcg tttttgtta accggggct 
      ccaaaaatc ccggtggtt acaccggca caaagggct 
      cggaatagg gcgattaat attgatagg ttcagtcgg 
      atttttttc cctttgctc ttcactgtc ctgaacggg 
      acgtattgg ataatgttc gctgagtat atttagatt 
      attagttga gagttaatg aatattaca ttatgtaga 
      cactgatta cgttaaata catgctgag tcaacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY