Status
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Gene information
General information
TC031093
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007727,TC007726
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 263269..270596
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031093
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04372

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031093 RA
      MNQADISKL VSQLRIKVN PRLRKFRSP GGPEARLDK 
      LRKTVTALV KHERIELNF PRADESRMY AERLISDAI 
      RYGDCHKET MEMADYWLV EKQLVHKLF KVLVPRFVN 
      YNTCYTRMY KAPTLYPET REKAVLELK GNPYPSLLP 
      ENYTNRNLI HNVLLDEAK KAYRTQKYA EIASKIDAA 
      EKNVEETRN FKNCGFLLF NWLPAGWWC SQAPRAHQY 
      GEEFENSPL EASAVRFVP LTQFRCFDG CLSHVPIAM 
      GAIASVLQW HCGSCSLIN PTEQLKCIR CGTSRQIQH 
      TNKGHDIAG SDPFAGGSS SANCTVIRR LKSTAKSSL 
      HDSGSLKRN CSSLVRNTV INTVLLLQR SASVKITEK 
      KPLGNNIHK SKSFQDLTR HNVCENCSV VFVSVARRC 
      LLCEGGVGM MNVAQSPCK NCASLTPIE QPNFRNYDI 
      LNCDRPLTC VCQRAKNNS FISLSSIES FSCSVAQQL 
      HCDPLIPSN IRTTASGIN KGHSPNYTW NKGAIGGNS 
      WTCKRCTLL NGPNRDVCE ACETPHTSD LNSNFNPSV 
      IIKVDNWAD NEALRNPSH NQRPSYRRS FSELPTSQN 
      LHVPNINRR SLGNEVLES VFPKSSTSM TDIQSSQHS 
      FDGITTRYS NLNLNRSTS DITCNEGAT ATTLYTYIG 
      ISEPDKDKT HIYENQGIV NAHNSKKGD FDSEQHDKI 
      LQALKSGSN SVNINWPFD KRKWTCRQC SFAYNDFNN 
      FKCDICKGP RTRPSLNEP CTVTVTEDR NSGATPLSL 
      NWSDSMELQ VPLATLDQD LDDDFQLLP GDDSPVDDQ 
      KWTCKKCTL VNSGRSLIC EACCGSKLR SLSMTGDMT 
      LRKGEFWSC VKCTLKNPL TAPICKACK TEKNSLDVV 
      ALSNRNPSP RHGSSKKYN KSSYAKVNH NQKVIVNRS 
      KTRINLMSD LDHSQSNAI KAWQCMICT FENTKSQII 
      CDMCQKIRE DIRTTPPEE PSRLSQEEL ANKHWNYIV 
      RYCKLKKQS YVDESFPPS PSSLYYNPS EVKDAHTVK 
      WKRLRDITV DDGPDSDLI WAVFRKPHP SDISQGVLG 
      NCWLLSALA VLAEREDLI RAVLITRDF CHQGVYQVR 
      LCKDGNWTT VLVDDYFPC DKKGHLFYS QAKRKQLWV 
      PLIEKAVAK IHGCYEALV SGRAIEGLA TLTGAPCES 
      IPLQASSIP APAEEELDT DLIWAQLLS SRQALFLMG 
      ASCGGGNMK VDEVEYQNK GLRPRHAYS LLDVRDVDG 
      YRLLKLRNP WGHFVWKGD WSDTSDKWT RALRMELMP 
      EGPQDGTFW ISFGDVLKY FDCIDICKA RNGWNEVRL 
      SGVLPPLSS QKHLSCILL TVLEPTEVD FTLFQEGQR 
      KSEKSQRSQ LDLCVVLFK ARNGSTIGS LVEHSKRQV 
      RGFVGCNKM LEAGEYVVV PLAFNHWHT GLEDLTAFP 
      RYVLAIHSS KKLLAEQLT PPDCILADS IISLTLARG 
      QRHEGREGM TAYYLTKGW AGLVVMVEN RHENKWIHV 
      KCDCQESYN VVSTRGTLK TVDSVPPLT RQVIIVLTQ 
      LEGSGGFSI AHRLTHRLA NSQGLYDWG PSGTCHDPE 
      LDYQTSGLH MPRLF
    CDS sequence
    • >TC031093 RA
      atgaatcaa gccgatatt tctaaacta gtgtcccaa 
      cttcgaatc aaagtaaat ccccgtttg cgaaaattc 
      cgttcgccg ggaggcccc gaagcacgt ttagacaag 
      ctccgaaaa acagttact gctttagtt aaacatgaa 
      cgaattgaa ttgaatttt cctcgagct gatgagtct 
      cgaatgtac gcagagagg ttaatttca gatgctata 
      cggtatgga gattgtcat aaagaaaca atggaaatg 
      gccgattat tggctggtt gaaaaacaa ctagtccac 
      aaattgttc aaagtctta gttcccaga tttgtaaat 
      tacaacacg tgttacaca agaatgtat aaagcaccg 
      actttgtat ccggaaaca cgggagaaa gctgtattg 
      gagttaaaa ggcaatcct tacccatcc ttactacct 
      gagaattac accaacagg aatttaatc cataatgtg 
      ttgttggat gaggctaaa aaggcctac aggacgcaa 
      aaatacgca gaaattgcg tccaaaatc gacgcggcc 
      gaaaaaaac gttgaagag actaggaat tttaaaaat 
      tgcggtttt ttgttgttt aactggttg cctgccggt 
      tggtggtgt tcgcaggcg ccaagggcg catcaatat 
      ggcgaagaa tttgaaaat agccctctt gaggcatca 
      gctgttcgc ttcgttccg ttgacacag tttcggtgt 
      tttgatggg tgtcttagc catgtgcca attgccatg 
      ggtgccatc gcctcggtg cttcaatgg cattgcggc 
      tcgtgcagc ttaataaac ccaaccgag cagctgaaa 
      tgcatcaga tgtggtacc agcagacag atccagcac 
      acaaacaaa ggccatgac attgctgga agcgaccca 
      tttgctggt ggtagcagc agtgcaaat tgtacagta 
      ataagacgc ttaaaatcc acagctaaa tcctcatta 
      cacgacagc ggctctcta aagcggaat tgttcgtcg 
      ttggtgcgt aacaccgtc ataaacacc gttctcttg 
      ctccaaaga agcgcctca gttaaaata accgaaaag 
      aaaccgctt ggcaataac atccacaaa tctaaaagt 
      ttccaagac ttgaccaga cacaacgtt tgtgaaaac 
      tgctcggtt gtttttgtt tcggttgca agacggtgt 
      cttttgtgt gaagggggc gtaggaatg atgaacgta 
      gctcaatca ccttgcaaa aattgtgct tcactcact 
      ccaatcgaa caaccaaat ttcaggaat tatgacata 
      cttaattgt gatagaccg ttaacgtgc gtgtgtcaa 
      agagcaaag aacaacagt ttcatttca ctttcgagc 
      attgaaagt ttcagttgt tcggttgca caacaatta 
      cactgtgat cctttaata ccaagcaac attcgaacc 
      actgcgagc ggaattaat aaagggcat agccctaat 
      tatacgtgg aataagggg gcgattggt gggaattcc 
      tggacttgt aaacgatgc actttacta aacggacct 
      aatagagat gtttgtgaa gcgtgcgaa acgccgcac 
      acctcagat ctaaacagc aattttaat ccgagcgtt 
      atcatcaag gttgataat tgggccgat aatgaagcg 
      ttgagaaac ccaagtcac aatcaacgg ccctcatat 
      cgccgctct ttttccgaa ctccccaca tcccagaat 
      ctccacgtc ccgaatatt aatcgtaga agtctcggt 
      aacgaagtg ttggagtca gtttttcca aaatcgagc 
      acttcaatg accgatata caatcctct caacattcc 
      ttcgatggc attacgact agatatagt aatcttaat 
      ttaaataga agtacaagt gatattacg tgcaatgaa 
      ggtgccact gccaccact ttatacacc tacattggc 
      atttcggaa ccggataaa gacaaaaca catatttac 
      gaaaatcaa ggaatcgtc aacgctcac aacagcaaa 
      aaaggcgat tttgatagt gaacaacac gacaaaata 
      ttgcaagcg ctgaaatcg ggaagcaat tcagttaac 
      ataaactgg ccgtttgat aaaagaaaa tggacttgt 
      cggcagtgt tcttttgca tacaatgat tttaataat 
      ttcaagtgc gatatttgt aaaggtccg agaaccaga 
      ccctcattg aatgaacct tgcactgtc actgtcaca 
      gaggatagg aatagtgga gccactcca ttgtctttg 
      aattggagc gactccatg gagctgcag gtgcccctg 
      gcgacgctg gaccaagac ctggacgac gacttccag 
      ctcttgcca ggcgatgat agtccagta gacgaccag 
      aaatggact tgtaaaaaa tgtacgctt gtaaatagc 
      ggtcgttcg ttaatttgt gaggcttgt tgcggttca 
      aaattgcgt tcgctttca atgactggc gatatgacg 
      cttcgaaaa ggcgaattt tggtcgtgt gttaaatgt 
      actttgaaa aatccgtta acggcgccg atttgtaaa 
      gcctgtaag actgagaaa aatagttta gatgtggtg 
      gcattgtcg aatcgaaat ccatcacca cgtcacggt 
      tcatccaaa aagtacaat aaaagttcg tacgccaaa 
      gtaaatcat aatcagaaa gttattgtg aatcgaagc 
      aaaactcga attaatttg atgtcggat ttagatcat 
      agtcagagt aatgcgatt aaagcgtgg cagtgtatg 
      atttgcact tttgaaaat accaagtct caaattatc 
      tgtgatatg tgccagaaa atacgtgaa gatattagg 
      accacgcct cctgaagaa ccgtcaagg ttgagtcaa 
      gaggaatta gctaataag cattggaat tatatagtg 
      agatattgt aagttgaag aagcagagt tacgttgat 
      gaatctttt ccgccgtca ccgagtagt ctttattat 
      aatccttcg gaagtcaaa gacgctcac acggttaaa 
      tggaaacga ctccgtgat ataacagtc gatgacggt 
      ccagactcc gatctaatt tgggccgtg tttcgaaaa 
      ccacaccct tccgacatt tcgcaagga gttttgggc 
      aattgttgg ctgttgagt gccttagcc gtgctggca 
      gaacgcgaa gatctcatt cgagctgtc ttgataacg 
      agagatttt tgccatcag ggagtttac caagtcaga 
      ctctgtaaa gatggcaat tggaccact gttctcgtt 
      gacgattac tttccatgt gataaaaag ggacacttg 
      ttttattcc caagcgaag cggaaacaa ctttgggtt 
      cctctaatc gaaaaagcc gtcgctaaa attcacggt 
      tgttacgaa gctctagtt tcggggcgt gcaattgaa 
      ggtttagca acactaact ggggcacct tgtgaatct 
      attcctctt caagcttct tcaattcct gctcctgct 
      gaggaggaa cttgacact gatctcatt tgggcacaa 
      cttttgtct tcacgtcag gcacttttt ttaatgggg 
      gcgtcatgt ggtggcggc aatatgaaa gtggacgaa 
      gtcgagtac caaaataag ggacttaga ccacgtcac 
      gcctattct ttgttagat gttagagat gtggatggt 
      tataggctt cttaaatta cgaaatcct tggggacat 
      ttcgtatgg aagggcgat tggtcggac acttccgac 
      aagtggacg cgggcgctc cgcatggaa ttaatgcca 
      gaagggcca caagatggt actttttgg atttcgttc 
      ggtgatgtt ttgaaatat ttcgactgc atcgatatt 
      tgtaaagca cggaatggt tggaacgaa gttagactt 
      tcgggtgtt ttaccgcca ctttcgtca cagaaacac 
      ttgtcttgt attttgctc acggttttg gaacccact 
      gaagtcgat tttacgctt tttcaagag ggccaacgc 
      aagtcggaa aaatctcaa cgatctcag ctcgattta 
      tgtgttgtt cttttcaaa gcacgaaac ggttcaacc 
      attggtagt ttagtggaa catagcaaa agacaggtt 
      cgaggtttc gtcggttgc aataaaatg ttagaagct 
      ggggaatat gttgttgtt ccgttagcc ttcaatcac 
      tggcatact ggtttggaa gatctcact gcattccca 
      agatatgtt ttagccatt cacagttca aagaaatta 
      ctggcagaa caattaact cctcctgat tgtattttg 
      gccgattcg ataatttcc ttgactttg gcacgtggc 
      caaagacat gaaggtcgt gaaggtatg accgcttat 
      tatttgacc aaaggatgg gcgggtttg gtcgtcatg 
      gttgaaaat cgccatgaa aataaatgg attcatgtg 
      aaatgtgat tgtcaagaa agttataat gtggtgtca 
      acacgtggt acgttgaaa acggtcgat tctgtgcct 
      cctttgacg cgacaagtt attattgtt ttaacgcaa 
      cttgagggt agtggcggt ttttctata gctcataga 
      cttacacat agacttgct aattcacag ggtttatat 
      gattggggg ccttctggc acttgtcac gatccagag 
      ctagattat cagaccagc ggtttgcat atgccacga 
      ttgttttga
    mRNA sequence
    • >TC031093 RA
      ttccaaaat aacctcaat ttgccacaa cttttttat 
      taattaaaa aatgaatca agccgatat ttctaaact 
      agtgtccca acttcgaat caaagtaaa tccccgttt 
      gcgaaaatt ccgttcgcc gggaggccc cgaagcacg 
      tttagacaa gctccgaaa aacagttac tgctttagt 
      taaacatga acgaattga attgaattt tcctcgagc 
      tgatgagtc tcgaatgta cgcagagag gttaatttc 
      agatgctat acggtatgg agattgtca taaagaaac 
      aatggaaat ggccgatta ttggctggt tgaaaaaca 
      actagtcca caaattgtt caaagtctt agttcccag 
      atttgtaaa ttacaacac gtgttacac aagaatgta 
      taaagcacc gactttgta tccggaaac acgggagaa 
      agctgtatt ggagttaaa aggcaatcc ttacccatc 
      cttactacc tgagaatta caccaacag gaatttaat 
      ccataatgt gttgttgga tgaggctaa aaaggccta 
      caggacgca aaaatacgc agaaattgc gtccaaaat 
      cgacgcggc cgaaaaaaa cgttgaaga gactaggaa 
      ttttaaaaa ttgcggttt tttgttgtt taactggtt 
      gcctgccgg ttggtggtg ttcgcaggc gccaagggc 
      gcatcaata tggcgaaga atttgaaaa tagccctct 
      tgaggcatc agctgttcg cttcgttcc gttgacaca 
      gtttcggtg ttttgatgg gtgtcttag ccatgtgcc 
      aattgccat gggtgccat cgcctcggt gcttcaatg 
      gcattgcgg ctcgtgcag cttaataaa cccaaccga 
      gcagctgaa atgcatcag atgtggtac cagcagaca 
      gatccagca cacaaacaa aggccatga cattgctgg 
      aagcgaccc atttgctgg tggtagcag cagtgcaaa 
      ttgtacagt aataagacg cttaaaatc cacagctaa 
      atcctcatt acacgacag cggctctct aaagcggaa 
      ttgttcgtc gttggtgcg taacaccgt cataaacac 
      cgttctctt gctccaaag aagcgcctc agttaaaat 
      aaccgaaaa gaaaccgct tggcaataa catccacaa 
      atctaaaag tttccaaga cttgaccag acacaacgt 
      ttgtgaaaa ctgctcggt tgtttttgt ttcggttgc 
      aagacggtg tcttttgtg tgaaggggg cgtaggaat 
      gatgaacgt agctcaatc accttgcaa aaattgtgc 
      ttcactcac tccaatcga acaaccaaa tttcaggaa 
      ttatgacat acttaattg tgatagacc gttaacgtg 
      cgtgtgtca aagagcaaa gaacaacag tttcatttc 
      actttcgag cattgaaag tttcagttg ttcggttgc 
      acaacaatt acactgtga tcctttaat accaagcaa 
      cattcgaac cactgcgag cggaattaa taaagggca 
      tagccctaa ttatacgtg gaataaggg ggcgattgg 
      tgggaattc ctggacttg taaacgatg cactttact 
      aaacggacc taatagaga tgtttgtga agcgtgcga 
      aacgccgca cacctcaga tctaaacag caattttaa 
      tccgagcgt tatcatcaa ggttgataa ttgggccga 
      taatgaagc gttgagaaa cccaagtca caatcaacg 
      gccctcata tcgccgctc tttttccga actccccac 
      atcccagaa tctccacgt cccgaatat taatcgtag 
      aagtctcgg taacgaagt gttggagtc agtttttcc 
      aaaatcgag cacttcaat gaccgatat acaatcctc 
      tcaacattc cttcgatgg cattacgac tagatatag 
      taatcttaa tttaaatag aagtacaag tgatattac 
      gtgcaatga aggtgccac tgccaccac tttatacac 
      ctacattgg catttcgga accggataa agacaaaac 
      acatattta cgaaaatca aggaatcgt caacgctca 
      caacagcaa aaaaggcga ttttgatag tgaacaaca 
      cgacaaaat attgcaagc gctgaaatc gggaagcaa 
      ttcagttaa cataaactg gccgtttga taaaagaaa 
      atggacttg tcggcagtg ttcttttgc atacaatga 
      ttttaataa tttcaagtg cgatatttg taaaggtcc 
      gagaaccag accctcatt gaatgaacc ttgcactgt 
      cactgtcac agaggatag gaatagtgg agccactcc 
      attgtcttt gaattggag cgactccat ggagctgca 
      ggtgcccct ggcgacgct ggaccaaga cctggacga 
      cgacttcca gctcttgcc aggcgatga tagtccagt 
      agacgacca gaaatggac ttgtaaaaa atgtacgct 
      tgtaaatag cggtcgttc gttaatttg tgaggcttg 
      ttgcggttc aaaattgcg ttcgctttc aatgactgg 
      cgatatgac gcttcgaaa aggcgaatt ttggtcgtg 
      tgttaaatg tactttgaa aaatccgtt aacggcgcc 
      gatttgtaa agcctgtaa gactgagaa aaatagttt 
      agatgtggt ggcattgtc gaatcgaaa tccatcacc 
      acgtcacgg ttcatccaa aaagtacaa taaaagttc 
      gtacgccaa agtaaatca taatcagaa agttattgt 
      gaatcgaag caaaactcg aattaattt gatgtcgga 
      tttagatca tagtcagag taatgcgat taaagcgtg 
      gcagtgtat gatttgcac ttttgaaaa taccaagtc 
      tcaaattat ctgtgatat gtgccagaa aatacgtga 
      agatattag gaccacgcc tcctgaaga accgtcaag 
      gttgagtca agaggaatt agctaataa gcattggaa 
      ttatatagt gagatattg taagttgaa gaagcagag 
      ttacgttga tgaatcttt tccgccgtc accgagtag 
      tctttatta taatccttc ggaagtcaa agacgctca 
      cacggttaa atggaaacg actccgtga tataacagt 
      cgatgacgg tccagactc cgatctaat ttgggccgt 
      gtttcgaaa accacaccc ttccgacat ttcgcaagg 
      agttttggg caattgttg gctgttgag tgccttagc 
      cgtgctggc agaacgcga agatctcat tcgagctgt 
      cttgataac gagagattt ttgccatca gggagttta 
      ccaagtcag actctgtaa agatggcaa ttggaccac 
      tgttctcgt tgacgatta ctttccatg tgataaaaa 
      gggacactt gttttattc ccaagcgaa gcggaaaca 
      actttgggt tcctctaat cgaaaaagc cgtcgctaa 
      aattcacgg ttgttacga agctctagt ttcggggcg 
      tgcaattga aggtttagc aacactaac tggggcacc 
      ttgtgaatc tattcctct tcaagcttc ttcaattcc 
      tgctcctgc tgaggagga acttgacac tgatctcat 
      ttgggcaca acttttgtc ttcacgtca ggcactttt 
      tttaatggg ggcgtcatg tggtggcgg caatatgaa 
      agtggacga agtcgagta ccaaaataa gggacttag 
      accacgtca cgcctattc tttgttaga tgttagaga 
      tgtggatgg ttataggct tcttaaatt acgaaatcc 
      ttggggaca tttcgtatg gaagggcga ttggtcgga 
      cacttccga caagtggac gcgggcgct ccgcatgga 
      attaatgcc agaagggcc acaagatgg tactttttg 
      gatttcgtt cggtgatgt tttgaaata tttcgactg 
      catcgatat ttgtaaagc acggaatgg ttggaacga 
      agttagact ttcgggtgt tttaccgcc actttcgtc 
      acagaaaca cttgtcttg tattttgct cacggtttt 
      ggaacccac tgaagtcga ttttacgct ttttcaaga 
      gggccaacg caagtcgga aaaatctca acgatctca 
      gctcgattt atgtgttgt tcttttcaa agcacgaaa 
      cggttcaac cattggtag tttagtgga acatagcaa 
      aagacaggt tcgaggttt cgtcggttg caataaaat 
      gttagaagc tggggaata tgttgttgt tccgttagc 
      cttcaatca ctggcatac tggtttgga agatctcac 
      tgcattccc aagatatgt tttagccat tcacagttc 
      aaagaaatt actggcaga acaattaac tcctcctga 
      ttgtatttt ggccgattc gataatttc cttgacttt 
      ggcacgtgg ccaaagaca tgaaggtcg tgaaggtat 
      gaccgctta ttatttgac caaaggatg ggcgggttt 
      ggtcgtcat ggttgaaaa tcgccatga aaataaatg 
      gattcatgt gaaatgtga ttgtcaaga aagttataa 
      tgtggtgtc aacacgtgg tacgttgaa aacggtcga 
      ttctgtgcc tcctttgac gcgacaagt tattattgt 
      tttaacgca acttgaggg tagtggcgg tttttctat 
      agctcatag acttacaca tagacttgc taattcaca 
      gggtttata tgattgggg gccttctgg cacttgtca 
      cgatccaga gctagatta tcagaccag cggtttgca 
      tatgccacg attgttttg aattttgtt atatattat 
      ggcctaaat gtgtcattt attgtttaa tcatctcga 
      ttcttattc ctcgaaaat tgtttattg aaaacgtat 
      tatacgaat aatgcagtg ttcagtttt tatgacgta 
      taaacgtga ttgttaacg taactacgt ttgttctct 
      cgttaagtg acttagtta cgttagctg tttggtgtt 
      taatagtac agtttcagt aacatgctt tttagattg 
      tcttgcaaa gtttgtgtg aaatcgtac attgttaag 
      aattaaa
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