Status
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Gene information
General information
TC007723 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 253109..254827
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007723
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007723 RA
      MTEKSFDKL QLNPWIIRQ CATIGVRSP TPIQTNCIP 
PILAGRDCI GAAKTGSGK TLAFALPIL QKLCEDPYG 
IFALILTPT RELAFQIAD QFAVIGKVM NLRHCVIVG 
GMDMVVQGK DLARKPHIV VATPGRLAD HLESCNTFN 
FNKLRFLVL DEADRLLGG HFDEQIKTI FQALPKERQ 
NLFFSATIT DTLEKLKDV TGKDVFFYE APAEVATVE 
QLEQNYVLC PKDVKDAYL VETIRTYRA TNDGNILIF 
TNTCKNCQV LSMTLNEVG FENVALHAM MKQQQRLAA 
LAKFKSNTI KILLATDVA SRGLDIPTV QLVINHNIP 
KIPKEYIHR VGRTARAGR NGKAISLVT PYDIKLLQA 
IEQHINTKL TEFKLDDSE VGKIFTQIS VTKSEAYIK 
LDEQDFYEK KMINKRKKW MMEGLDPDE EEEKFLKTI 
KKSKKAKKK KQKNDN
    CDS sequence
    • >TC007723 RA
      atgaccgag aaatcgttc gacaagctc cagttgaac 
ccttggatc attcgccag tgcgccact ataggtgtg 
cgcagccct acacccatc cagaccaac tgtattccc 
ccaattctc gccggtagg gactgtatc ggggctgct 
aaaaccggc agtggcaaa accctagca tttgccttg 
cccattctc cagaaatta tgcgaggat ccttacggt 
attttcgct cttatttta acccccaca cgagaactg 
gcgtttcaa atcgctgat cagtttgca gtcattggc 
aaagtgatg aatttgcgc cattgtgtc attgtgggg 
ggtatggat atggtggtt cagggcaaa gacttagcc 
cgaaaaccc cacattgtg gtggcaacg cctggccgc 
ttagctgac catttagaa agttgcaac acgtttaat 
tttaataaa ttgaggttt ttggtgctt gatgaggcc 
gatcgtttg ttagggggg cactttgac gaacaaatc 
aaaacgatt tttcaagca ttgcctaag gagcgccaa 
aatttattt ttctcagcc acgatcacc gatacttta 
gaaaaactg aaagatgtg acagggaaa gatgttttt 
ttctatgaa gccccggct gaagtagca actgttgaa 
caattggaa caaaactat gtattgtgc cccaaagat 
gtgaaagac gcttatttg gttgagaca attaggacg 
tatcgtgct acaaatgac ggaaatatc cttattttc 
actaacact tgcaagaat tgtcaagtg ttgtcaatg 
actttaaat gaggtcggt tttgaaaat gtggccctt 
catgccatg atgaaacaa caacagaga ttagcagct 
ttggctaaa tttaaatca aacacaatt aaaattctt 
ctagccact gatgttgca agtagaggt cttgatatt 
cccaccgtt caattagta attaatcac aatattcct 
aaaataccg aaggaatac atacatagg gttggtaga 
actgccagg gctgggaga aacggcaaa gcgataagt 
ttggtcacc ccttacgac atcaaattg ttacaagcg 
atcgaacaa cacattaat acgaaatta actgaattt 
aaattggac gatagcgaa gttggtaaa attttcacg 
caaatttcg gtaaccaaa agcgaggct tatattaaa 
ctcgacgaa caagatttt tacgaaaag aaaatgata 
aataagagg aaaaagtgg atgatggaa gggcttgat 
ccagacgag gaagaagaa aaattttta aagacaatc 
aaaaaaagc aagaaggcg aaaaagaag aaacaaaag 
aatgacaat tga
    mRNA sequence
    • >TC007723 RA
      caaaaaaat atccaaaac tctagtaaa aacctaaca 
ctttgccca aaaatcgta aaaaacgat tcggtcgtg 
tacacaatc ggtcgcaat gtcggggaa cctaacccc 
tctgaatat taagtttgt gtttttcgc gtaataatg 
accgagaaa tcgttcgac aagctccag ttgaaccct 
tggatcatt cgccagtgc gccactata ggtgtgcgc 
agccctaca cccatccag accaactgt attccccca 
attctcgcc ggtagggac tgtatcggg gctgctaaa 
accggcagt ggcaaaacc ctagcattt gccttgccc 
attctccag aaattatgc gaggatcct tacggtatt 
ttcgctctt attttaacc cccacacga gaactggcg 
tttcaaatc gctgatcag tttgcagtc attggcaaa 
gtgatgaat ttgcgccat tgtgtcatt gtggggggt 
atggatatg gtggttcag ggcaaagac ttagcccga 
aaaccccac attgtggtg gcaacgcct ggccgctta 
gctgaccat ttagaaagt tgcaacacg tttaatttt 
aataaattg aggtttttg gtgcttgat gaggccgat 
cgtttgtta ggggggcac tttgacgaa caaatcaaa 
acgattttt caagcattg cctaaggag cgccaaaat 
ttatttttc tcagccacg atcaccgat actttagaa 
aaactgaaa gatgtgaca gggaaagat gtttttttc 
tatgaagcc ccggctgaa gtagcaact gttgaacaa 
ttggaacaa aactatgta ttgtgcccc aaagatgtg 
aaagacgct tatttggtt gagacaatt aggacgtat 
cgtgctaca aatgacgga aatatcctt attttcact 
aacacttgc aagaattgt caagtgttg tcaatgact 
ttaaatgag gtcggtttt gaaaatgtg gcccttcat 
gccatgatg aaacaacaa cagagatta gcagctttg 
gctaaattt aaatcaaac acaattaaa attcttcta 
gccactgat gttgcaagt agaggtctt gatattccc 
accgttcaa ttagtaatt aatcacaat attcctaaa 
ataccgaag gaatacata catagggtt ggtagaact 
gccagggct gggagaaac ggcaaagcg ataagtttg 
gtcacccct tacgacatc aaattgtta caagcgatc 
gaacaacac attaatacg aaattaact gaatttaaa 
ttggacgat agcgaagtt ggtaaaatt ttcacgcaa 
atttcggta accaaaagc gaggcttat attaaactc 
gacgaacaa gatttttac gaaaagaaa atgataaat 
aagaggaaa aagtggatg atggaaggg cttgatcca 
gacgaggaa gaagaaaaa tttttaaag acaatcaaa 
aaaagcaag aaggcgaaa aagaagaaa caaaagaat 
gacaattga aattttaaa tatttttgt aaattgagc 
acaaataaa ttttctaaa tcgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY