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Gene information
General information
TC007716 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 98016..103971
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007716
    iBeetle screen iB_04370

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007716 RA
      MANRPCKIC NINVSLGTW MSHVHNPKH LSKTPGAIM 
      IKGSFTSDN VKELNDYLS RFGIIESYK ITTEDDRTA 
      VEYVFKNTV SHSVIIHCD HSIFDQELE VSWATKKFK 
      SNNSAPGRV LTKIPLGGS TETAPSSSK ITADPEAVK 
      KALLASIKE PPTFAEQIQ LLQKQLQVG KAEIEQNRN 
      IIYDDLNLA LSQTYNSYD ICFFGSSIT GLDVQGSDL 
      DVYIDNVRP VTKPEVAVL KTIRFLIFK SRKFCDVLL 
      ISGAKTPII KCIHTKTQI CCDINVKNR LSVRNSELI 
      KYYLTLDAK IKPLMIFVK FWADLYGLK KVNFFSSYA 
      LYMMVIYYL QQPPYSVPT VLTLQRNAP PEIVDIWNC 
      GFDEIDFTS PALEKTTIL DLLVGFFRF YGHFDYVSN 
      VIAPFYGAI IDKASFLKP HDLPRCYHT YMSQSVALA 
      VNSGVCIQD PFEHSRNVS ASVPLQCIG RFTNMCRMA 
      EKLTQNGET ELLYKLLTT RTEPVFSFE INKVSEKSD 
      QEWINLVRE ITMKFLSRV LNLKVSETL MNASPDVTY 
      NASGQQNIW DNRINIYKN LTQLPSNTS EIDKEIAVT 
      ECILKYFPQ NLNTSLILQ LKFKENPTR VEVSVKQMG 
      KCKQFFHIY HYIKVKLVK FINLAAQHP EAGPESVMV 
      QTTQPSIST CSIENYPDK QVEAVENQE GVDNNETST 
      KPVKEVEEE EEEDVPADF FDDFSNQDF MDGLDIVDA 
      WDEASKPVT TDPPIDIKV EKLERKHSP IRFSPTERK 
      SSEKRKKLA RSRSRTPTR RYHTSWKRS RSRDRIRER 
      RDPEKTKRD INRDKIKCA RDRELKIVS DKLKIVETG 
      LVPPGTEMD FDVGTVLPE KRRSNTGSK TRYVSRRSS 
      SRETRKRYS RSPVYRPSR IRSRKRTSS IESGVSERE 
      LWIRSSRKR TRSRSRDSP RKKREETKK KSFLEEIRD 
      KLNETSTRT VVSVPPVQY TMGYGNAVP VPAPVPVPV 
      PAPTQYFPQ TQYDQNFFI GDSFQMNRP PQNFNMMPN 
      QGNVMPIPQ RQIGQVAPI QPGIIATPT PPPPPAIVD 
      NNTNVQEID KKTTDDIDK LFADKKISL SDFLTITAK 
      CEVQSSNPD HLQKKIKVI SRCQDAIKV MSDERKFTG 
      RLLVKSSPM EQINKNDVK YQSPLRRIP VVRFQFTTP 
      SKTIEGMDG FSVAVDKIL KSVGIVKES ISNEVIETG 
      QKSQNSSFA DEDTAALTL KATMLGAKK EVSCDECKK 
      RKQKTFVEV GSQCADNKE TCAVGIQVN EEELTPTKV 
      VKNESIAFL TPAQLLGKN KESDKNKES PTSPQKVEK 
      KTPEVKKKP QPSPRGRGG FRKGGPPAR NNERPFYEE 
      KFDYYENPS FGHRRYGGP SRFPQQHYE EEAEQYEEE 
      EEGDEFYGD YTDFNHQPF QGRQRFGPR F
    CDS sequence
    • >TC007716 RA
      atggcaaac agaccttgc aaaatatgc aacattaac 
      gtttcgttg ggtacgtgg atgtcacac gtccacaat 
      ccaaaacac ttatctaag acacccgga gccattatg 
      attaaaggc agtttcact agcgacaat gtgaaggaa 
      ttaaacgat tacttgtcc cgctttggt ataatcgaa 
      agttataaa ataacaacc gaggatgat cgaacggcc 
      gttgaatac gtttttaag aatacagta tcacactct 
      gtgatcatt cactgcgat catagcatt ttcgaccag 
      gaattggaa gtgagttgg gccacaaaa aaatttaaa 
      tcgaataat tccgcacct ggacgagtt cttacaaaa 
      atcccgctt ggcggttcc acagaaacc gccccttcc 
      tcttccaaa ataactgcg gaccccgaa gccgttaaa 
      aaggcccta cttgcaagt ataaaagag cccccaacg 
      ttcgccgaa cagatacaa ctcctccaa aagcaactc 
      caagtcggt aaagcagaa atcgaacaa aaccgcaac 
      attatttac gatgacttg aatctagca ctctcgcag 
      acttacaat tcgtatgac atttgtttt tttgggtct 
      agtatcacc ggtttggac gttcaaggc agtgatttg 
      gacgtttat attgataat gtgcgacct gtgacaaag 
      cctgaagtg gctgtgttg aaaactatc cgatttttg 
      atttttaaa agtcgcaaa ttttgcgat gttttactc 
      atttcaggt gccaaaaca ccgattatt aagtgcata 
      catacgaaa acgcagatt tgttgcgat attaacgtt 
      aaaaatcga ctttcggta cgaaacagc gaattgatc 
      aagtactac ttgaccttg gacgcgaaa atcaaaccc 
      ttgatgatt tttgtcaag ttttgggcc gatttatac 
      ggtttgaaa aaagttaat tttttctca agctatgcc 
      ctctacatg atggtaatt tattacttg caacaacca 
      ccttacagt gtaccaact gtgcttacg ctgcaacgt 
      aacgctcct cctgaaata gtagatatt tggaattgt 
      ggttttgac gaaattgat ttcacaagt ccggccctg 
      gaaaaaacg acaattctt gatctattg gtcgggttt 
      tttcgtttt tacggtcat tttgactac gtttcaaat 
      gtcattgca ccattttat ggcgctatt attgacaaa 
      gcttcgttt ttgaagccg catgatttg ccaagatgt 
      tatcacact tatatgagt caaagtgtg gcacttgct 
      gttaattcg ggagtttgt attcaggac ccgtttgag 
      cattcgagg aacgtttcg gcctcagtg cctcttcaa 
      tgtattgga agatttacg aatatgtgt cgcatggct 
      gaaaaattg actcaaaac ggcgaaact gaacttttg 
      tataaatta ttgacaact aggactgaa ccggttttt 
      agctttgaa attaacaaa gtttccgaa aaaagtgac 
      caagaatgg attaatttg gtccgagag ataacaatg 
      aaatttcta agtcgtgtt ttaaacttg aaagtgagc 
      gaaacactg atgaatgca tcacctgat gtcacttat 
      aatgcctca ggccaacaa aatatttgg gataacaga 
      attaatatt tacaagaat ttgacacaa ttgccgtca 
      aatacgtcc gaaattgat aaagaaatt gccgttact 
      gaatgtatt ttaaaatat tttccacaa aacttaaac 
      acgtcccta attctgcaa ttgaagttc aaggaaaat 
      ccgacaaga gtcgaagtt tcagtcaaa cagatgggc 
      aagtgtaag caatttttc cacatttat cactacatt 
      aaagtcaaa ctggtgaaa tttatcaat ttggcggca 
      caacatcct gaagccggt cctgagtct gtgatggtg 
      caaacaacc cagccatcg attagcaca tgtagtatt 
      gagaattac cctgacaaa caggttgaa gcggtcgaa 
      aatcaagaa ggtgttgac aataatgaa acatcaaca 
      aaacctgtc aaagaagtg gaagaggag gaagaagaa 
      gacgttccg gctgatttt ttcgacgat ttttctaat 
      caggatttt atggatggt ttagatatt gttgatgca 
      tgggatgaa gcttcaaaa cctgtaaca accgaccca 
      ccaattgat ataaaagtg gaaaaactt gaacgaaaa 
      cattcccca attcgtttc agtccaaca gaacgtaaa 
      tcatcggaa aaacgtaaa aaactagca cgttctaga 
      tcacgaacg ccaactcga cgttaccac acgtcgtgg 
      aagcgctcc agatcacgt gacaggatc cgggaacgt 
      cgtgatccc gaaaaaacc aaacgtgac atcaatcgg 
      gacaaaatc aagtgtgcc agagacaga gaattaaaa 
      atagttagt gataaattg aaaattgtc gaaacaggg 
      ttggtacca cctggaaca gaaatggac tttgacgtt 
      ggtacagtt cttccggaa aaacggcgt tcaaacacc 
      ggaagtaaa acgcgatat gtttctaga cgtagttca 
      agtcgcgaa acacggaaa cgttattca cgaagtcca 
      gtttatagg ccttctcgg attcgttcg cgtaaacgc 
      acttctagt atcgagtcc ggtgtttcg gagcgcgaa 
      ctttggatt cgatcttcg cgtaaaagg acgcgatca 
      cgttccagg gattccccg cgaaagaaa cgcgaagaa 
      acaaagaag aaatcattt ttggaagaa atccgggat 
      aagttgaat gaaacaagc actcggact gtggtttct 
      gttccgcca gttcagtat actatggga tatgggaat 
      gcagttccg gttcctgct ccagttccg gttccagtt 
      ccagctcca actcagtac ttcccccaa acacaatac 
      gatcagaat ttttttatt ggcgattcg tttcaaatg 
      aatcgacca ccacaaaat tttaatatg atgccaaat 
      caaggtaat gtaatgcca attccacaa agacaaata 
      gggcaggtt gcaccaatc caaccagga attattgcc 
      acacctacg ccaccaccg ccacctgcg attgttgat 
      aacaacaca aacgtgcaa gagattgat aaaaagaca 
      actgatgat attgataaa ctttttgcc gataagaag 
      atttccctt tcggatttt ttgactatt acagcgaaa 
      tgtgaagtc caatcgtca aatcctgat catcttcag 
      aaaaaaatc aaggtaata tcgcgctgt caggacgca 
      atcaaggtg atgagcgac gaacgtaaa tttactgga 
      agattgtta gttaagtca tcgccaatg gaacaaatt 
      aataaaaat gatgtaaaa tatcaatcg ccattacga 
      agaattccg gtggttcgt ttccaattt acaacacca 
      tcaaaaacc attgaagga atggacggt ttttcggtt 
      gctgttgat aagattttg aaaagtgtt ggtattgtg 
      aaagaatca ataagtaac gaagtgatt gaaactggt 
      cagaaatca caaaacagt tcatttgct gatgaagac 
      acagccgct ttaacatta aaagctaca atgttgggt 
      gcaaaaaaa gaagtgagc tgtgatgag tgcaaaaaa 
      cgcaaacaa aagacattt gtggaagtg ggatcgcag 
      tgtgcggat aataaagag acttgtgct gtgggaatc 
      caagtaaat gaagaagaa ttaacaccg acgaaagtt 
      gtaaagaat gagtcgatt gcgtttttg acaccggca 
      caactcttg ggaaaaaat aaggaaagt gataagaat 
      aaagaaagt ccaacatca ccacaaaaa gtggagaag 
      aaaacacca gaagttaag aaaaaacca caaccgtca 
      ccaagaggc agaggagga tttagaaag ggaggacca 
      ccagctcgt aataatgag cgcccattc tacgaagaa 
      aaatttgat tattacgaa aatcctagt ttcggtcat 
      cgaagatat gggggaccg tcacgtttt ccccaacaa 
      cactatgaa gaagaagcg gaacagtat gaagaagaa 
      gaagaaggt gatgaattc tatggcgac tataccgat 
      tttaatcat cagccgttt caaggaagg cagaggttt 
      ggacctcga ttctga
    mRNA sequence
    • >TC007716 RA
      ccacaaaaa aatattttt taagacact tcactactc 
      cagactgtg cttcagatt aattataat tcatctgca 
      ttttttcct gtgaccatg tggacggat tattgtttt 
      ttgtgtaaa tttggctaa taacaagac atgatctca 
      gtttctgat catttttca ggaccagtt tttaacctt 
      tttgttgca attcttttt aatatataa cttgagtta 
      aaataagtg cttcagtac ttgtggaat tcttgcatt 
      tgtgagttg tcgcgacct tgagaaagt gcaagaaat 
      aatggcgtg ttgaaattg tacaaacat gccctaagt 
      ccaactaaa aggtgactt aatgaactg aagatggca 
      aacagacct tgcaaaata tgcaacatt aacgtttcg 
      ttgggtacg tggatgtca cacgtccac aatccaaaa 
      cacttatct aagacaccc ggagccatt atgattaaa 
      ggcagtttc actagcgac aatgtgaag gaattaaac 
      gattacttg tcccgcttt ggtataatc gaaagttat 
      aaaataaca accgaggat gatcgaacg gccgttgaa 
      tacgttttt aagaataca gtatcacac tctgtgatc 
      attcactgc gatcatagc attttcgac caggaattg 
      gaagtgagt tgggccaca aaaaaattt aaatcgaat 
      aattccgca cctggacga gttcttaca aaaatcccg 
      cttggcggt tccacagaa accgcccct tcctcttcc 
      aaaataact gcggacccc gaagccgtt aaaaaggcc 
      ctacttgca agtataaaa gagccccca acgttcgcc 
      gaacagata caactcctc caaaagcaa ctccaagtc 
      ggtaaagca gaaatcgaa caaaaccgc aacattatt 
      tacgatgac ttgaatcta gcactctcg cagacttac 
      aattcgtat gacatttgt ttttttggg tctagtatc 
      accggtttg gacgttcaa ggcagtgat ttggacgtt 
      tatattgat aatgtgcga cctgtgaca aagcctgaa 
      gtggctgtg ttgaaaact atccgattt ttgattttt 
      aaaagtcgc aaattttgc gatgtttta ctcatttca 
      ggtgccaaa acaccgatt attaagtgc atacatacg 
      aaaacgcag atttgttgc gatattaac gttaaaaat 
      cgactttcg gtacgaaac agcgaattg atcaagtac 
      tacttgacc ttggacgcg aaaatcaaa cccttgatg 
      atttttgtc aagttttgg gccgattta tacggtttg 
      aaaaaagtt aattttttc tcaagctat gccctctac 
      atgatggta atttattac ttgcaacaa ccaccttac 
      agtgtacca actgtgctt acgctgcaa cgtaacgct 
      cctcctgaa atagtagat atttggaat tgtggtttt 
      gacgaaatt gatttcaca agtccggcc ctggaaaaa 
      acgacaatt cttgatcta ttggtcggg ttttttcgt 
      ttttacggt cattttgac tacgtttca aatgtcatt 
      gcaccattt tatggcgct attattgac aaagcttcg 
      tttttgaag ccgcatgat ttgccaaga tgttatcac 
      acttatatg agtcaaagt gtggcactt gctgttaat 
      tcgggagtt tgtattcag gacccgttt gagcattcg 
      aggaacgtt tcggcctca gtgcctctt caatgtatt 
      ggaagattt acgaatatg tgtcgcatg gctgaaaaa 
      ttgactcaa aacggcgaa actgaactt ttgtataaa 
      ttattgaca actaggact gaaccggtt tttagcttt 
      gaaattaac aaagtttcc gaaaaaagt gaccaagaa 
      tggattaat ttggtccga gagataaca atgaaattt 
      ctaagtcgt gttttaaac ttgaaagtg agcgaaaca 
      ctgatgaat gcatcacct gatgtcact tataatgcc 
      tcaggccaa caaaatatt tgggataac agaattaat 
      atttacaag aatttgaca caattgccg tcaaatacg 
      tccgaaatt gataaagaa attgccgtt actgaatgt 
      attttaaaa tattttcca caaaactta aacacgtcc 
      ctaattctg caattgaag ttcaaggaa aatccgaca 
      agagtcgaa gtttcagtc aaacagatg ggcaagtgt 
      aagcaattt ttccacatt tatcactac attaaagtc 
      aaactggtg aaatttatc aatttggcg gcacaacat 
      cctgaagcc ggtcctgag tctgtgatg gtgcaaaca 
      acccagcca tcgattagc acatgtagt attgagaat 
      taccctgac aaacaggtt gaagcggtc gaaaatcaa 
      gaaggtgtt gacaataat gaaacatca acaaaacct 
      gtcaaagaa gtggaagag gaggaagaa gaagacgtt 
      ccggctgat tttttcgac gatttttct aatcaggat 
      tttatggat ggtttagat attgttgat gcatgggat 
      gaagcttca aaacctgta acaaccgac ccaccaatt 
      gatataaaa gtggaaaaa cttgaacga aaacattcc 
      ccaattcgt ttcagtcca acagaacgt aaatcatcg 
      gaaaaacgt aaaaaacta gcacgttct agatcacga 
      acgccaact cgacgttac cacacgtcg tggaagcgc 
      tccagatca cgtgacagg atccgggaa cgtcgtgat 
      cccgaaaaa accaaacgt gacatcaat cgggacaaa 
      atcaagtgt gccagagac agagaatta aaaatagtt 
      agtgataaa ttgaaaatt gtcgaaaca gggttggta 
      ccacctgga acagaaatg gactttgac gttggtaca 
      gttcttccg gaaaaacgg cgttcaaac accggaagt 
      aaaacgcga tatgtttct agacgtagt tcaagtcgc 
      gaaacacgg aaacgttat tcacgaagt ccagtttat 
      aggccttct cggattcgt tcgcgtaaa cgcacttct 
      agtatcgag tccggtgtt tcggagcgc gaactttgg 
      attcgatct tcgcgtaaa aggacgcga tcacgttcc 
      agggattcc ccgcgaaag aaacgcgaa gaaacaaag 
      aagaaatca tttttggaa gaaatccgg gataagttg 
      aatgaaaca agcactcgg actgtggtt tctgttccg 
      ccagttcag tatactatg ggatatggg aatgcagtt 
      ccggttcct gctccagtt ccggttcca gttccagct 
      ccaactcag tacttcccc caaacacaa tacgatcag 
      aattttttt attggcgat tcgtttcaa atgaatcga 
      ccaccacaa aattttaat atgatgcca aatcaaggt 
      aatgtaatg ccaattcca caaagacaa atagggcag 
      gttgcacca atccaacca ggaattatt gccacacct 
      acgccacca ccgccacct gcgattgtt gataacaac 
      acaaacgtg caagagatt gataaaaag acaactgat 
      gatattgat aaacttttt gccgataag aagatttcc 
      ctttcggat tttttgact attacagcg aaatgtgaa 
      gtccaatcg tcaaatcct gatcatctt cagaaaaaa 
      atcaaggta atatcgcgc tgtcaggac gcaatcaag 
      gtgatgagc gacgaacgt aaatttact ggaagattg 
      ttagttaag tcatcgcca atggaacaa attaataaa 
      aatgatgta aaatatcaa tcgccatta cgaagaatt 
      ccggtggtt cgtttccaa tttacaaca ccatcaaaa 
      accattgaa ggaatggac ggtttttcg gttgctgtt 
      gataagatt ttgaaaagt gttggtatt gtgaaagaa 
      tcaataagt aacgaagtg attgaaact ggtcagaaa 
      tcacaaaac agttcattt gctgatgaa gacacagcc 
      gctttaaca ttaaaagct acaatgttg ggtgcaaaa 
      aaagaagtg agctgtgat gagtgcaaa aaacgcaaa 
      caaaagaca tttgtggaa gtgggatcg cagtgtgcg 
      gataataaa gagacttgt gctgtggga atccaagta 
      aatgaagaa gaattaaca ccgacgaaa gttgtaaag 
      aatgagtcg attgcgttt ttgacaccg gcacaactc 
      ttgggaaaa aataaggaa agtgataag aataaagaa 
      agtccaaca tcaccacaa aaagtggag aagaaaaca 
      ccagaagtt aagaaaaaa ccacaaccg tcaccaaga 
      ggcagagga ggatttaga aagggagga ccaccagct 
      cgtaataat gagcgccca ttctacgaa gaaaaattt 
      gattattac gaaaatcct agtttcggt catcgaaga 
      tatggggga ccgtcacgt tttccccaa caacactat 
      gaagaagaa gcggaacag tatgaagaa gaagaagaa 
      ggtgatgaa ttctatggc gactatacc gattttaat 
      catcagccg tttcaagga aggcagagg tttggacct 
      cgattctga attttgttt tttctgttg ttaagagaa 
      tctaaactt gtattttaa tataagcta gattttagt 
      ttcttatgt ttttgaaat gttactcaa gaaataaac 
      ttgtgtatg tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY