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Gene information
General information
TC007708 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 49006..53498
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007708
    iBeetle screen iB_04369

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007708 RA
      MDPVGPWSA YASYNRLAG VQGDHHPTG APPTTTAQL 
      LPGSFLSPT PVGYETVFS PLFHHAGTK PTAHYVAQH 
      RTKQSDSDY HQQPFFEQG AGWQQNSPF GILPHESVV 
      PTTKASTYE NFNAHFAAA QSLNHLNSQ LAAAKSRAQ 
      SPATTKPPP AFFQTATTS NSCIVSTPS NSTVSKEYR 
      VPQTPRTYL TSPNPQRPT LKQPQIQTK AQTKIYPEL 
      NQQNERQRN NEEPQSQSS PISFSLNYS GKRTPQFQN 
      SNYFPQNTT SNNTNGPDC NNVVVPRRP SPLQAHSQP 
      SPIGHAPSP AYPMYNSPM NSISSPQQT SNQVTPPSP 
      LDVSVPRPT SQPTNVVAY PSVITRTEK TFPQNNQNC 
      WEERRKFPT GNQANNYNT LPEQRVGQQ GYFEANSGH 
      QMALQDLSS CRGDPMSIV KNLQQQQQS CQVAQVEVK 
      QEVKPPVKR RKSNEKAPS EVPRIPPPA HSSGAQGQP 
      QNGAYFDFD RWNLPPPSS KIFTSQTIH QQHQGLMVP 
      HPHGHHPPP PLPYFAPFH LPPHPSEFP STVELTPIN 
      NYSEQNTQP SSHFPQQQQ EDQPKVVVP NIEEELNFL 
      AQGGSTTKK PMKPSEKPG SGFMSSYLK FLQGERDSS 
      PPPATRGNR KQSSWTRSK PQINNETKP ESNGTTPTV 
      IQPSQPPVV TRLSQGDPQ DDPRYFPLP KERKRNSFE 
      SDSDGISSD DDFFGNNKK FSNYDRKDK PRKKTKSNT 
      ERKKVKKPK KEEEVIPRR ESTKRAAKE KINLQHLIK 
      VDDDLEEPP EFQDSDSDP AWTPQAKED GEEIIPISK 
      KSRKSSAKK RRNLITAAA QGAGIHEID GYVSDNSKK 
      NKGSKNKQN APPTLEDNI AASLSNNVV TNDDNPFKP 
      GEFVVIRSE LSQDWPAIW RVDGKTLLQ KYEPFEQNG 
      VTLYRNIST YTSWTPESK KQYISIPVA YKVQNHVET 
      IVEFLRNEM TLIDQDYQQ KCMRDCEGY QDNFEVYIQ 
      TLISQALDS NFLTEIFQE KDEYFLSNV KTIDDITDS 
      KKRKLLSLL RWPPAVQAA VCTWPCFNV IREVNPSDI 
      HTKSCAACG RLEVAVRVL MYGQPYNST TLEGCQPDP 
      KAMNEKNFL MCRICATRV ELSNKVTHQ KYLMYIECA 
      KRVSEKRTS DPNKDTTCI LNELLADEN WLNQLFLEV 
      RTSWAEIDC IEYSLKLKN SVYTQ
    CDS sequence
    • >TC007708 RA
      atggatccg gtgggccca tggtcggct tacgcctct 
      tataatcgt cttgcgggt gttcagggg gaccaccac 
      ccaacgggc gctccgccc actaccact gctcaattg 
      ttacctgga agctttctc tccccaact cctgtgggt 
      tacgagacc gttttcagt ccccttttt catcacgct 
      ggaactaaa cccacagcc cactatgtc gctcagcac 
      cgtaccaaa cagtccgac agtgactac caccaacag 
      ccttttttt gaacaaggg gctggatgg caacaaaat 
      agccctttt ggaattttg ccgcatgaa agtgtggtt 
      ccaaccacg aaagcgtcg acgtatgaa aactttaac 
      gctcatttc gcagccgct caaagcctc aatcattta 
      aattcgcaa ttagcggca gcgaaatct cgagctcaa 
      agccctgcc accacaaaa ccgcctcca gcgttcttc 
      caaaccgcc accacttca aactcatgt atagtatca 
      acaccgtct aattcaacc gtttcgaaa gagtataga 
      gtacctcaa acacccaga acttatctc acttcaccc 
      aacccccaa agacctacc cttaaacaa ccccaaatc 
      caaacgaaa gctcaaacg aaaatctac ccagaacta 
      aaccaacaa aatgaaaga cagcgaaac aacgaagag 
      ccccaaagt caatcatcc ccaatcagt ttctcgcta 
      aactactct ggaaaacgt acaccacaa tttcaaaat 
      tcgaattac tttccacaa aataccacc tccaacaat 
      acaaacggc cccgattgc aacaacgtc gtagtcccc 
      cgtagaccc agccctctt caagcccac tcccaaccc 
      tcaccaatt ggccacgcc ccaagtccc gcctatcca 
      atgtacaac agtccaatg aactcaatc tcttcccct 
      caacaaact tccaatcaa gttacacca ccgtcacca 
      ctggacgtc tctgttcca aggcccacc tctcaacca 
      accaacgtc gttgcctat ccttctgtc ataacacgt 
      accgaaaaa accttcccg caaaataat caaaattgt 
      tgggaagaa cgacggaaa tttccgaca ggaaatcaa 
      gccaataat tacaacacg ttaccagaa caaagagta 
      ggacaacaa gggtatttt gaggcgaat agcggccat 
      cagatggcg ctacaggat ttgtcaagt tgtcgtggc 
      gatccaatg agtattgtc aaaaatttg caacaacag 
      caacaaagt tgtcaagtg gctcaagtc gaggttaaa 
      caggaggtt aaaccgccg gttaagagg aggaaaagt 
      aatgagaaa gcgccaagt gaagtgcca aggatacca 
      ccgccagca cacagttct ggggcgcaa ggacagcca 
      caaaacggc gcctatttc gactttgac aggtggaat 
      ctgcctcct ccttcttca aaaatcttt accagccaa 
      acaatccat caacagcac caaggtttg atggtacca 
      catccacat ggtcaccat ccaccacct ccattgcca 
      tattttgca cctttccac ttaccccct cacccatca 
      gaatttcca tcaaccgtc gaactaacc ccaattaac 
      aattattcg gaacaaaat acacaacca tcgtcacat 
      tttccacaa caacaacaa gaagatcaa ccgaaagtt 
      gttgtaccc aacattgaa gaagaatta aattttctt 
      gcacaaggt ggtagtacg acaaaaaaa ccgatgaaa 
      ccgagcgag aaacccggt tcgggtttt atgagtagt 
      tatcttaag ttccttcaa ggagaacgt gatagttct 
      ccgccccca gctacacgg ggtaataga aaacagtct 
      tcatggaca cgttcaaaa ccccaaata aacaacgag 
      acaaaaccc gaaagtaat ggaacaact ccaacagtt 
      atacagccc tcacagcca ccggtggtt acaaggtta 
      tcgcaaggg gaccctcaa gacgatccc aggtatttt 
      cccttacct aaagaacgc aaaagaaat agttttgaa 
      agtgacagc gatggaatt agttcagac gatgatttc 
      tttggaaat aacaagaag tttagtaat tacgatcga 
      aaagacaag ccgcggaaa aaaacaaaa agtaacaca 
      gaacggaag aaagtaaag aaacccaaa aaggaagaa 
      gaagttata ccgcgaaga gaatcaaca aaacgagca 
      gcgaaagaa aaaataaat ttgcaacat cttatcaaa 
      gtcgacgat gatcttgaa gaacctcca gagtttcaa 
      gattcggat tcggatccg gcgtggacg ccacaagcg 
      aaagaagat ggcgaggaa attataccg atttcgaaa 
      aaatcacgg aaatctagc gccaaaaaa cgaaggaat 
      cttatcaca gcagctgca caaggtgcc ggcatacat 
      gaaatcgac ggttacgtc agcgacaat tccaagaaa 
      aataaagga agtaaaaat aaacaaaat gcgccacct 
      acgctcgaa gataatatt gcggcttct ttatctaat 
      aatgttgtt acgaatgat gacaatccg ttcaagcct 
      ggtgaattt gttgtgatt cgatctgaa ctgagtcaa 
      gactggcct gcgatttgg cgtgttgat ggaaaaaca 
      ttacttcag aaatatgaa ccgtttgaa caaaacggt 
      gtcacgtta tacaggaat atttcaacg tatacgtca 
      tggactcct gaaagtaaa aagcaatac atctcgata 
      ccagtggct tacaaagtc caaaatcat gtggaaacg 
      atcgttgaa tttttacgt aacgaaatg acgcttatc 
      gatcaggac taccaacag aaatgtatg agagattgt 
      gagggttat caggacaat ttcgaagtg tacatacag 
      acattaatt tcacaagca ttggatagt aatttcctg 
      acggaaatc ttccaagag aaagatgag tatttcctg 
      tctaatgtg aaaactata gacgacata actgatagc 
      aaaaagagg aaattgttg tcgctgctg cgatggccg 
      ccagcggtc caagcagca gtgtgtacg tggccatgt 
      tttaatgtg attcgcgaa gtaaatccg tctgatatt 
      catacgaaa agttgtgca gcttgtggt cgattggaa 
      gtcgctgtt cgagtgtta atgtacggc caaccgtat 
      aattcaaca actttggaa ggttgtcaa ccggatcca 
      aaagcaatg aatgaaaaa aatttttta atgtgtagg 
      atatgtgca acgcgtgtc gaactttca aataaagtg 
      actcatcaa aagtatttg atgtatatt gaatgtgcg 
      aaacgtgtc agtgaaaaa cggactagt gatccaaat 
      aaggatacg acttgtata ttaaatgag ttgttagcc 
      gatgagaat tggttaaat cagttgttt ttggaggtg 
      cgtacttcg tgggcggag attgactgt atagagtac 
      agtttaaaa ttgaaaaat tcagtgtac acgcagtga
    mRNA sequence
    • >TC007708 RA
      ctcatctat tgttaggaa aacacacta aaatggcga 
      aataagttt ggtagctaa acgatgttg taaatacgc 
      aaatgcctg acttaatta tgaatgttt ttacttttt 
      caactcatc agtcacgtg tactgaacc ctagagttg 
      taacagtgc cgttgttat aattttgtg ttgtttatt 
      ttgataatt cgtgctcag tgggacttc aaagacatg 
      aagccatat ttacgaaaa ccggacaag agatataat 
      tggtgaatc gtctttttc tcaatgaga aatgggatg 
      gctaaacca ctgaaactt agtcatgga tccggtggg 
      cccatggtc ggcttacgc ctcttataa tcgtcttgc 
      gggtgttca gggggacca ccacccaac gggcgctcc 
      gcccactac cactgctca attgttacc tggaagctt 
      tctctcccc aactcctgt gggttacga gaccgtttt 
      cagtcccct ttttcatca cgctggaac taaacccac 
      agcccacta tgtcgctca gcaccgtac caaacagtc 
      cgacagtga ctaccacca acagccttt ttttgaaca 
      aggggctgg atggcaaca aaatagccc ttttggaat 
      tttgccgca tgaaagtgt ggttccaac cacgaaagc 
      gtcgacgta tgaaaactt taacgctca tttcgcagc 
      cgctcaaag cctcaatca tttaaattc gcaattagc 
      ggcagcgaa atctcgagc tcaaagccc tgccaccac 
      aaaaccgcc tccagcgtt cttccaaac cgccaccac 
      ttcaaactc atgtatagt atcaacacc gtctaattc 
      aaccgtttc gaaagagta tagagtacc tcaaacacc 
      cagaactta tctcacttc acccaaccc ccaaagacc 
      tacccttaa acaacccca aatccaaac gaaagctca 
      aacgaaaat ctacccaga actaaacca acaaaatga 
      aagacagcg aaacaacga agagcccca aagtcaatc 
      atccccaat cagtttctc gctaaacta ctctggaaa 
      acgtacacc acaatttca aaattcgaa ttactttcc 
      acaaaatac cacctccaa caatacaaa cggccccga 
      ttgcaacaa cgtcgtagt cccccgtag acccagccc 
      tcttcaagc ccactccca accctcacc aattggcca 
      cgccccaag tcccgccta tccaatgta caacagtcc 
      aatgaactc aatctcttc ccctcaaca aacttccaa 
      tcaagttac accaccgtc accactgga cgtctctgt 
      tccaaggcc cacctctca accaaccaa cgtcgttgc 
      ctatccttc tgtcataac acgtaccga aaaaacctt 
      cccgcaaaa taatcaaaa ttgttggga agaacgacg 
      gaaatttcc gacaggaaa tcaagccaa taattacaa 
      cacgttacc agaacaaag agtaggaca acaagggta 
      ttttgaggc gaatagcgg ccatcagat ggcgctaca 
      ggatttgtc aagttgtcg tggcgatcc aatgagtat 
      tgtcaaaaa tttgcaaca acagcaaca aagttgtca 
      agtggctca agtcgaggt taaacagga ggttaaacc 
      gccggttaa gaggaggaa aagtaatga gaaagcgcc 
      aagtgaagt gccaaggat accaccgcc agcacacag 
      ttctggggc gcaaggaca gccacaaaa cggcgccta 
      tttcgactt tgacaggtg gaatctgcc tcctccttc 
      ttcaaaaat ctttaccag ccaaacaat ccatcaaca 
      gcaccaagg tttgatggt accacatcc acatggtca 
      ccatccacc acctccatt gccatattt tgcaccttt 
      ccacttacc ccctcaccc atcagaatt tccatcaac 
      cgtcgaact aaccccaat taacaatta ttcggaaca 
      aaatacaca accatcgtc acattttcc acaacaaca 
      acaagaaga tcaaccgaa agttgttgt acccaacat 
      tgaagaaga attaaattt tcttgcaca aggtggtag 
      tacgacaaa aaaaccgat gaaaccgag cgagaaacc 
      cggttcggg ttttatgag tagttatct taagttcct 
      tcaaggaga acgtgatag ttctccgcc cccagctac 
      acggggtaa tagaaaaca gtcttcatg gacacgttc 
      aaaacccca aataaacaa cgagacaaa acccgaaag 
      taatggaac aactccaac agttataca gccctcaca 
      gccaccggt ggttacaag gttatcgca aggggaccc 
      tcaagacga tcccaggta ttttccctt acctaaaga 
      acgcaaaag aaatagttt tgaaagtga cagcgatgg 
      aattagttc agacgatga tttctttgg aaataacaa 
      gaagtttag taattacga tcgaaaaga caagccgcg 
      gaaaaaaac aaaaagtaa cacagaacg gaagaaagt 
      aaagaaacc caaaaagga agaagaagt tataccgcg 
      aagagaatc aacaaaacg agcagcgaa agaaaaaat 
      aaatttgca acatcttat caaagtcga cgatgatct 
      tgaagaacc tccagagtt tcaagattc ggattcgga 
      tccggcgtg gacgccaca agcgaaaga agatggcga 
      ggaaattat accgatttc gaaaaaatc acggaaatc 
      tagcgccaa aaaacgaag gaatcttat cacagcagc 
      tgcacaagg tgccggcat acatgaaat cgacggtta 
      cgtcagcga caattccaa gaaaaataa aggaagtaa 
      aaataaaca aaatgcgcc acctacgct cgaagataa 
      tattgcggc ttctttatc taataatgt tgttacgaa 
      tgatgacaa tccgttcaa gcctggtga atttgttgt 
      gattcgatc tgaactgag tcaagactg gcctgcgat 
      ttggcgtgt tgatggaaa aacattact tcagaaata 
      tgaaccgtt tgaacaaaa cggtgtcac gttatacag 
      gaatatttc aacgtatac gtcatggac tcctgaaag 
      taaaaagca atacatctc gataccagt ggcttacaa 
      agtccaaaa tcatgtgga aacgatcgt tgaattttt 
      acgtaacga aatgacgct tatcgatca ggactacca 
      acagaaatg tatgagaga ttgtgaggg ttatcagga 
      caatttcga agtgtacat acagacatt aatttcaca 
      agcattgga tagtaattt cctgacgga aatcttcca 
      agagaaaga tgagtattt cctgtctaa tgtgaaaac 
      tatagacga cataactga tagcaaaaa gaggaaatt 
      gttgtcgct gctgcgatg gccgccagc ggtccaagc 
      agcagtgtg tacgtggcc atgttttaa tgtgattcg 
      cgaagtaaa tccgtctga tattcatac gaaaagttg 
      tgcagcttg tggtcgatt ggaagtcgc tgttcgagt 
      gttaatgta cggccaacc gtataattc aacaacttt 
      ggaaggttg tcaaccgga tccaaaagc aatgaatga 
      aaaaaattt tttaatgtg taggatatg tgcaacgcg 
      tgtcgaact ttcaaataa agtgactca tcaaaagta 
      tttgatgta tattgaatg tgcgaaacg tgtcagtga 
      aaaacggac tagtgatcc aaataagga tacgacttg 
      tatattaaa tgagttgtt agccgatga gaattggtt 
      aaatcagtt gtttttgga ggtgcgtac ttcgtgggc 
      ggagattga ctgtataga gtacagttt aaaattgaa 
      aaattcagt gtacacgca gtgaatttt gttctcgaa 
      agacttgaa taaaatttg aaggacttt aatatcaaa 
      attgtgtac attatattt gatgagagt ttatataaa 
      actaaaaaa tgtattgtt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY