Status
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Gene information
General information
TC007705 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 25256..32266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007705
    iBeetle screen iB_04368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007705 RA
      MSAESPRRS RSQTRGGSS VTVAPQQVT DPTILDSVA 
      GFINEVVPT AYNVPNEVD DKIQWAHFE QLDQDEATW 
      GSIYDADNA VPPPLILVL GYTTGIQIW VIPATGEAT 
      EILSFRHSI LRVLRLIPT PFKTGGDNV DLFASSRPL 
      IALCDSISN YSTLTFRSL KGGVVDPVK QIQFKTSIL 
      NVLANRHSI VVSFAEKIA VFDAFTLEN RISVTTCYL 
      SPGIQQNPL ALGARWLAY AEKRLIANN RSGGGNEGE 
      GVQSYTATV LHAAKSLGR GIREFTESV AGSLTGNPN 
      FKPGSSTSS PQAGGVADV PQKGIVTIL DIENKTMSP 
      QENVIPQDA VIAHFVAHT EAIVCLSFD PSGMLLLTA 
      DKRGHDFHV FRIQPHPGG PALAAVHHL YVLHRGDTS 
      AKVQDMCFS PDSRWVTVS TLRGTTHVF PVTPYGGPI 
      SVRTHATAH VVNKMSRYQ RSAGLTLEG RSSSPVSLT 
      ETPVNSLFP YRNPRFPPY PQPTIVNPL AQIRQPVYV 
      QNTTGALSQ RTHPGRQRL SSSSEENIT LRVVACFAS 
      PRAWMDVSK GGPRETSHK PQIKPVESL FVISCYGTL 
      IQYDLEPHP SSSLPKERV CNDTPIELK VCAKAQWSL 
      HRRLHTADV PLPLSEDNL NFIAQSVPL YKKTKPDHN 
      DDHWLSQVE IVTHLGPHR RLWMGPQFT FKTYTITNG 
      TVLETKSVD VRRSKPVNM PVSKANAIL IESGSASSC 
      EQLFLDTYH KACEEVGSA GETRLKEDL ADAMLESPG 
      IRGGDTGGH CVIVSMKPS STVAKVVNP LGTVVTVQS 
      DDETTLEQQ VIEDAVIHE NCDEALFRP VVAPKAVFY 
      SETKPKPCP PDHILTKSL EANTEVAVK IINHKSNNE 
      KNNRLSSKH IKSRESSPK TKTIKKSCG KIEENKTKI 
      KKEDKVQDV LDCVCVDLP QFEDVPMKK SPDSSLFES 
      ILKFEDAED CSEKMTNAL NKTTDPEIT CSSEEEKLS 
      TRKARKPKT KLGVKITKE KENASLVEI DVPPIKRSW 
      NIVAASKPP KEKIIDLDL PEIESVESF AVNKLAKKE 
      NLIDIDVEV KNDELLKID NKSSDEIGS SPDTTESDD 
      SSKVPPIVE DENLAPAQN SQTSKSSRK KSKKKRGEQ 
      SAELNNKDI EDMSSASSF SSASRASPT PSCSDNLLH 
      FPGDSSSSM
    CDS sequence
    • >TC007705 RA
      atgtctgct gaatcacct cgacggtcc agatcccag 
      acgagaggg gggagtagc gtcactgtc gcacctcaa 
      caagtcacg gaccccacg attttggac agcgtcgct 
      ggatttatc aacgaggtc gtaccgaca gcgtataat 
      gtacctaac gaagttgac gataagata caatgggca 
      cattttgaa caactagac caggacgaa gccacatgg 
      gggtccatt tacgatgct gataatgcc gttccaccg 
      cccctgata ctcgtcttg ggatacact acaggcata 
      cagatttgg gtgattcca gcaacgggc gaagccact 
      gaaatctta tcatttcgt catagcatt cttcgtgtt 
      ctccgattg attccgact ccgttcaaa actggcgga 
      gacaatgtt gatttgttt gcatcatcg cgtcctcta 
      atcgctctt tgtgactct atttcaaat tacagcact 
      ctgactttt cggtcactt aaaggagga gttgtagat 
      ccggtaaaa cagatacag tttaaaacg tcaatcttg 
      aacgtttta gcaaatcgt cattcgatt gtggtgagt 
      tttgctgaa aaaatcgcc gtcttcgat gcatttact 
      ttggagaat cgtatcagt gtgactact tgttatttg 
      agccctggt atccaacag aatccttta gctttaggt 
      gcaagatgg ttggcgtac gctgaaaaa aggctaata 
      gcgaataat cggagtgga ggtggcaat gaaggggaa 
      ggagtacaa agttatacc gctactgta ttacatgca 
      gcaaagtct ttgggtcgt ggcattcgg gaattcacc 
      gaatcagtg gctggaagt ttaacgggg aatcccaat 
      tttaaacct ggttcatca acgagtagc ccccaagcc 
      ggaggagta gccgatgtg ccccaaaaa ggcatcgtt 
      acaattctc gacattgaa aacaagaca atgagtcca 
      caagagaat gttattccg caagatgct gtgatagcc 
      cattttgta gcccacact gaagctatt gtttgttta 
      tcgtttgat ccgagcgga atgttactt ttaacagcc 
      gataaacgt ggtcatgat tttcacgtg tttcggata 
      caaccccat cctgggggc cctgctttg gctgcagtc 
      catcacttg tatgtgtta catcgtggt gatacatcg 
      gccaaagtt caagatatg tgtttttca ccggattcg 
      cgttgggtg actgtatct acacttcgg ggaactact 
      catgtattt ccggtgaca ccttacggt ggtccgatt 
      agtgtacga acacatgct acagcacat gttgttaat 
      aagatgtca aggtatcaa cgatcagct ggattgact 
      ttggaaggt agatcaagt agtccagta tcattgact 
      gaaacacca gtcaattcg ttgtttccc tatcgcaat 
      ccaagattt ccaccttat ccacagccg actattgtc 
      aatccactt gctcaaatc agacaaccg gtttatgta 
      caaaataca acgggagca ttgtctcaa agaacccat 
      cctggaaga caacgattg tcttcgtcg tcagaagag 
      aatattact ttgagggtt gtagcgtgt tttgcctca 
      cctagagca tggatggat gtctcaaaa gggggacca 
      agagagact tcgcacaag ccccaaatc aaaccggtg 
      gagtccttg ttcgtaatc tcgtgctac ggaacgtta 
      atccagtac gatttggaa ccacatcca tcttcttcg 
      ctcccaaaa gaacgcgta tgtaacgat accccaatt 
      gaattgaaa gtatgtgca aaagcccaa tggtctctt 
      catcgtcgc cttcatacc gctgacgtt cccttacca 
      ttatccgaa gacaactta aattttata gctcaaagc 
      gtgcctctt tacaaaaag actaaaccc gatcataac 
      gacgatcat tggttgtct caagttgaa attgtgaca 
      catctagga ccccatagg aggctttgg atgggaccc 
      caatttacg ttcaaaacc tataccatc acaaatggt 
      actgtttta gaaacgaaa tcagttgat gtgagacgt 
      tcaaaaccg gttaatatg ccggtgagc aaagctaat 
      gcgatttta attgaatcg gggtctgca agcagttgc 
      gagcaattg tttttggac acatatcat aaggcgtgt 
      gaggaggtg ggaagtgct ggggagaca agacttaaa 
      gaggatttg gcagatgcc atgttagaa tcgccagga 
      attcgagga ggagataca ggtgggcat tgcgtgatc 
      gtttcaatg aaaccatca tcgacagtt gccaaagtc 
      gtgaatccg ttaggcacc gttgtaaca gtccaatct 
      gatgatgaa acaacgcta gaacagcaa gttattgaa 
      gatgctgtt atacatgaa aactgcgat gaagcatta 
      tttagaccg gtggtggca ccaaaagcc gttttttat 
      tcagaaacc aaaccgaaa ccttgtcct ccagatcac 
      atactgacc aaaagtctc gaagccaac actgaagtc 
      gccgtcaaa atcatcaat cataaaagc aacaatgaa 
      aaaaataat agactatct tccaaacat atcaagtcg 
      agagaaagc tcacctaaa actaaaaca atcaaaaaa 
      agttgcggc aaaattgag gagaataag accaaaatc 
      aagaaggaa gacaaagtg caagatgtt ttggattgt 
      gtgtgcgtt gatttgccc cagtttgag gatgtgccg 
      atgaagaaa agccccgac tcatctcta ttcgaaagc 
      attttaaaa tttgaagat gctgaagat tgcagcgaa 
      aaaatgaca aacgctttg aataaaaca accgatccg 
      gaaataact tgttcttcc gaagaagag aaactgtct 
      acacgtaaa gcacgcaaa cctaaaact aagcttgga 
      gtgaaaata acaaaagaa aaagagaat gccagttta 
      gtggaaatc gatgttcca ccaataaaa aggtcttgg 
      aatattgtg gcggcctca aaaccaccc aaagaaaaa 
      atcatcgat ttggatctt cccgaaatt gaaagtgtg 
      gaaagtttt gcagtaaat aaacttgcg aaaaaagag 
      aatttgatt gatattgac gtggaggtg aaaaatgat 
      gagttgttg aagattgat aataaatct tcggatgag 
      atcggatca tcaccagat acgaccgaa agtgacgat 
      tcaagtaaa gtaccaccg attgtcgag gatgaaaat 
      cttgcgccg gcgcaaaac tcgcaaacg tcgaaaagt 
      tctcggaaa aagtcaaag aaaaaacgt ggtgaacaa 
      tcggcagag ttgaacaac aaagacatt gaagatatg 
      agttctgcg agtagtttc agttcagca agtcgggca 
      tcaccaacg ccctcctgc agtgataat ctgttacat 
      tttccaggt gattccagc agtagcatg tga
    mRNA sequence
    • >TC007705 RA
      taacttttt tctctcctt atttaaagc cataaaatg 
      atgcatcaa aagcttgat taaagccaa aataatcat 
      ttattaaaa ttattatta ttaaattac tcactaact 
      gtcccacca tttgctaaa attaaatcg cccgctacc 
      actcgacca atgttgtcc aatcagctg atttctgtt 
      gttgtgaca gtgacagtt gtgaagttc tttgtactc 
      tgctgatgc atattgatt tatagtgat ttgaaaccg 
      tctgaagcc cttgtttgc gactttgat ggtgagttt 
      cagtacttg taccctttc catgtgttc atctttcgg 
      tgttaatcc cctatttct gacgtcata tctgtttag 
      atcttcggc agcgaaatg acgcagttt gggaaatga 
      aatgttgat ttgcatcga aaacaagcg accaaatta 
      tgtctgctg aatcacctc gacggtcca gatcccaga 
      cgagagggg ggagtagcg tcactgtcg cacctcaac 
      aagtcacgg accccacga ttttggaca gcgtcgctg 
      gatttatca acgaggtcg taccgacag cgtataatg 
      tacctaacg aagttgacg ataagatac aatgggcac 
      attttgaac aactagacc aggacgaag ccacatggg 
      ggtccattt acgatgctg ataatgccg ttccaccgc 
      ccctgatac tcgtcttgg gatacacta caggcatac 
      agatttggg tgattccag caacgggcg aagccactg 
      aaatcttat catttcgtc atagcattc ttcgtgttc 
      tccgattga ttccgactc cgttcaaaa ctggcggag 
      acaatgttg atttgtttg catcatcgc gtcctctaa 
      tcgctcttt gtgactcta tttcaaatt acagcactc 
      tgacttttc ggtcactta aaggaggag ttgtagatc 
      cggtaaaac agatacagt ttaaaacgt caatcttga 
      acgttttag caaatcgtc attcgattg tggtgagtt 
      ttgctgaaa aaatcgccg tcttcgatg catttactt 
      tggagaatc gtatcagtg tgactactt gttatttga 
      gccctggta tccaacaga atcctttag ctttaggtg 
      caagatggt tggcgtacg ctgaaaaaa ggctaatag 
      cgaataatc ggagtggag gtggcaatg aaggggaag 
      gagtacaaa gttataccg ctactgtat tacatgcag 
      caaagtctt tgggtcgtg gcattcggg aattcaccg 
      aatcagtgg ctggaagtt taacgggga atcccaatt 
      ttaaacctg gttcatcaa cgagtagcc cccaagccg 
      gaggagtag ccgatgtgc cccaaaaag gcatcgtta 
      caattctcg acattgaaa acaagacaa tgagtccac 
      aagagaatg ttattccgc aagatgctg tgatagccc 
      attttgtag cccacactg aagctattg tttgtttat 
      cgtttgatc cgagcggaa tgttacttt taacagccg 
      ataaacgtg gtcatgatt ttcacgtgt ttcggatac 
      aaccccatc ctgggggcc ctgctttgg ctgcagtcc 
      atcacttgt atgtgttac atcgtggtg atacatcgg 
      ccaaagttc aagatatgt gtttttcac cggattcgc 
      gttgggtga ctgtatcta cacttcggg gaactactc 
      atgtatttc cggtgacac cttacggtg gtccgatta 
      gtgtacgaa cacatgcta cagcacatg ttgttaata 
      agatgtcaa ggtatcaac gatcagctg gattgactt 
      tggaaggta gatcaagta gtccagtat cattgactg 
      aaacaccag tcaattcgt tgtttccct atcgcaatc 
      caagatttc caccttatc cacagccga ctattgtca 
      atccacttg ctcaaatca gacaaccgg tttatgtac 
      aaaatacaa cgggagcat tgtctcaaa gaacccatc 
      ctggaagac aacgattgt cttcgtcgt cagaagaga 
      atattactt tgagggttg tagcgtgtt ttgcctcac 
      ctagagcat ggatggatg tctcaaaag ggggaccaa 
      gagagactt cgcacaagc cccaaatca aaccggtgg 
      agtccttgt tcgtaatct cgtgctacg gaacgttaa 
      tccagtacg atttggaac cacatccat cttcttcgc 
      tcccaaaag aacgcgtat gtaacgata ccccaattg 
      aattgaaag tatgtgcaa aagcccaat ggtctcttc 
      atcgtcgcc ttcataccg ctgacgttc ccttaccat 
      tatccgaag acaacttaa attttatag ctcaaagcg 
      tgcctcttt acaaaaaga ctaaacccg atcataacg 
      acgatcatt ggttgtctc aagttgaaa ttgtgacac 
      atctaggac cccatagga ggctttgga tgggacccc 
      aatttacgt tcaaaacct ataccatca caaatggta 
      ctgttttag aaacgaaat cagttgatg tgagacgtt 
      caaaaccgg ttaatatgc cggtgagca aagctaatg 
      cgattttaa ttgaatcgg ggtctgcaa gcagttgcg 
      agcaattgt ttttggaca catatcata aggcgtgtg 
      aggaggtgg gaagtgctg gggagacaa gacttaaag 
      aggatttgg cagatgcca tgttagaat cgccaggaa 
      ttcgaggag gagatacag gtgggcatt gcgtgatcg 
      tttcaatga aaccatcat cgacagttg ccaaagtcg 
      tgaatccgt taggcaccg ttgtaacag tccaatctg 
      atgatgaaa caacgctag aacagcaag ttattgaag 
      atgctgtta tacatgaaa actgcgatg aagcattat 
      ttagaccgg tggtggcac caaaagccg ttttttatt 
      cagaaacca aaccgaaac cttgtcctc cagatcaca 
      tactgacca aaagtctcg aagccaaca ctgaagtcg 
      ccgtcaaaa tcatcaatc ataaaagca acaatgaaa 
      aaaataata gactatctt ccaaacata tcaagtcga 
      gagaaagct cacctaaaa ctaaaacaa tcaaaaaaa 
      gttgcggca aaattgagg agaataaga ccaaaatca 
      agaaggaag acaaagtgc aagatgttt tggattgtg 
      tgtgcgttg atttgcccc agtttgagg atgtgccga 
      tgaagaaaa gccccgact catctctat tcgaaagca 
      ttttaaaat ttgaagatg ctgaagatt gcagcgaaa 
      aaatgacaa acgctttga ataaaacaa ccgatccgg 
      aaataactt gttcttccg aagaagaga aactgtcta 
      cacgtaaag cacgcaaac ctaaaacta agcttggag 
      tgaaaataa caaaagaaa aagagaatg ccagtttag 
      tggaaatcg atgttccac caataaaaa ggtcttgga 
      atattgtgg cggcctcaa aaccaccca aagaaaaaa 
      tcatcgatt tggatcttc ccgaaattg aaagtgtgg 
      aaagttttg cagtaaata aacttgcga aaaaagaga 
      atttgattg atattgacg tggaggtga aaaatgatg 
      agttgttga agattgata ataaatctt cggatgaga 
      tcggatcat caccagata cgaccgaaa gtgacgatt 
      caagtaaag taccaccga ttgtcgagg atgaaaatc 
      ttgcgccgg cgcaaaact cgcaaacgt cgaaaagtt 
      ctcggaaaa agtcaaaga aaaaacgtg gtgaacaat 
      cggcagagt tgaacaaca aagacattg aagatatga 
      gttctgcga gtagtttca gttcagcaa gtcgggcat 
      caccaacgc cctcctgca gtgataatc tgttacatt 
      ttccaggtg attccagca gtagcatgt gaagtgagg 
      aagtgccac taataactt tccagtgat tgtagttat 
      ttttctttt ttttttttt agtttaaaa gagaggcga 
      gagcaatat ttttcattg ttaaagatt gtgataatg 
      gagaaagta tagtttttg tttaattgc atttcgtaa 
      actccatca taacaattt tgcgttcgg ttttgtcaa 
      ttcttccat tttggtgcc ccgctagaa tagaatcaa 
      aacatttgc atagcgtaa ggacatgta gctagtcgt 
      ttgatttaa ttctagata actcaaggg acaagtgat 
      agactatag ttagacgaa atgtgatgt tataatcaa 
      tgatatgat gtttaggaa attattttg tcgccagac 
      actttgcta catattttt ataaatacg attagaaag 
      agttagttt ctcagactt attttgtac gattcgttc 
      ttatattta tactagtag gacgtaata aaatgttac 
      ttctgga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY