Status
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Gene information
General information
TC032730
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007694
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 689959..700809
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032730
    iBeetle screen iB_04366

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032730 RA
      MLVRLISSK TLLRSTQHI SFLPATCNF STKIEYKPI 
      RSVLVANRG EIAVRVFRA CNELGIKSV AIYSKEDRA 
      HIHRLKADE SYLVGEGLP PVQAYLNIP EIIKICKDN 
      GIDAVHPGY GFLSERADF AQAVIDAGI RFIGPSPKV 
      VHQMGDKVA AREAAIAAG VPIVPGTDG PVTTKEEAK 
      EFCVKHGLP VIFKAAYGG GGRGMRVVR KMEEVEENF 
      QRASSEALS AFGNGAMFI EKFIERPRH IEVQLMGDK 
      AGNVVHLYE RDCSVQRRH QKVVEMAPA PHLDINVRN 
      KMTDLAVKL AKHVGYENA GTVEFLCDE KGNFYFIEV 
      NARLQVEHT VTEEITGID LVQTQIRVA EGMTLPEMG 
      IEQENIKPN GFAIQCRVT TEDPAKNFQ PDTGRIEVF 
      RSGEGMGIR LDGASAFAG AIISPYYDS LLVKVIAHS 
      RDLQASCAK MNRALREFR VRGVKTNVP FLLNVLENQ 
      KFLNGAVDT YFIDENPQL FNFPQTQNR AQKLLNYLG 
      QVLVNGPQT PLATSSKPS DIKPHLPEV SQDAQPPKG 
      FRDIYKQQG PEAFAKAVR NHKGLLLMD TTYRDAHQS 
      LLATRVRSH DLLRISPFV THNFNQLYS LENWGGATF 
      DVALRFLHE CPWERLEEM RKLIPNIPF QMLLRGANA 
      VGYTNYPDN VVFKFCELA VQTGMDVFR VFDSLNYLP 
      NLIVGMEAA GKAGGIVEA AISYSGDVS NPNKTKYDL 
      KYYLNIAEE LVKAGTHVL AIKDMAGLL KPAAGRMLV 
      SALRDKYPD LPIHIHTHD TSGAGVAAM LACAEAGAD 
      VVDVAVDSM SGLTSQPSM GAVVASLQG TQLDTKLDL 
      SKVSEYSAY WEQTRTLYA PFECTTTMK SGNADVYIN 
      EIPGGQYTN LQFQAYSLG LGDFFEDVK KAYAEANIL 
      LGDIIKVTP SSKVVGDLA QFMVQNKLS PEDVLNKAE 
      ELSFPKSVV EYLQGHIGQ PYGGFPEPL RSKVLRDMP 
      RIEGRPGAS LPPLEFDKL KTDLKETFP HATDRDVMS 
      AALYPQVTN EYLAFKEQY GPVDKLDTR IFLVGPKVG 
      EEFEVTIEK GKTLGIKTL AMAEDLTEN GEREVFFEL 
      NGTLRSVLI RDKDAGKEI HVHPKADKA NKKQVGAPM 
      PGTVIDIRV KVGEKVEKG TPLVILSAM KMETVVQSP 
      IAGVIKTLD VNMGMKLEA EDLMMTVE
    CDS sequence
    • >TC032730 RA
      atgctcgtc cgcctcatc agctcgaaa accctcctc 
      cgctcgacc cagcacatc tccttcctc cctgcaaca 
      tgcaacttc tccacaaaa atcgaatac aaaccaatc 
      agaagcgtc ctcgtggca aatcgtggc gaaatcgcc 
      gttcgtgtt ttccgcgct tgtaacgaa cttggaatc 
      aaatcggtg gcaatctac tccaaagag gacagagcc 
      cacattcac agactcaaa gccgatgaa agttaccta 
      gtgggtgaa ggcctacca cctgtacaa gcctacctt 
      aacataccc gaaattatc aaaatttgt aaagacaac 
      ggcattgac gctgtacat ccaggctat ggatttctg 
      tcagaaagg gccgacttt gcacaagct gttatcgat 
      gccggaatt agattcatc ggaccgtcg ccaaaggtc 
      gtgcaccag atgggggac aaagtggcc gccagagag 
      gcggctatt gctgcaggt gttcctatt gttccgggg 
      acagatgga cctgtcacc acaaaggag gaagccaag 
      gagttttgc gtcaaacac ggattacct gtcattttt 
      aaggcggct tatgggggc ggcggccgt ggcatgcgc 
      gtcgtccgc aagatggaa gaagtggag gagaacttc 
      cagagagca tcgtccgaa gccctaagt gccttcggt 
      aacggtgcc atgtttatt gaaaaattc atcgaacgt 
      ccaagacac atcgaagtg caactaatg ggtgataag 
      gccggtaat gtggtgcac ctctacgag cgcgattgt 
      tccgtccaa cggaggcac cagaaggtg gtggaaatg 
      gccccagcc ccccatttg gacatcaac gtccgtaat 
      aaaatgacc gatttggcc gtaaaactt gccaaacat 
      gtgggctat gaaaatgcc ggcactgtt gaattttta 
      tgcgatgaa aaaggcaat ttctacttt atcgaagtg 
      aacgctcga ctccaagtc gaacacacg gtcacggag 
      gaaatcacc ggaattgat ttggtgcaa acgcaaatc 
      cgggtcgct gaaggaatg acgcttccg gagatggga 
      atcgagcaa gaaaatatc aaaccaaac ggttttgcg 
      attcaatgc cgggtgacc accgaagac ccagccaaa 
      aacttccaa ccggatacg ggaaggatc gaagtgttc 
      cggtccggt gaagggatg gggatacgt ctagatggc 
      gcgtcggcg tttgcagga gcaattatt tcgccctat 
      tatgattcg ctcctagtc aaggtcatt gcacattcg 
      agggacttg caagcgtcg tgcgctaaa atgaaccga 
      gcattgagg gaatttcgc gttcgaggt gttaaaact 
      aacgtccct tttcttcta aacgtgttg gaaaatcaa 
      aagtttttg aatggagct gtcgatact tatttcatt 
      gacgaaaat ccgcaactt tttaatttc ccacagacg 
      caaaatcgg gcacaaaaa ttgctcaat tatttaggg 
      caggtgctt gttaatgga ccacaaact cccctagct 
      acgtcaagt aaaccgtcg gatattaaa ccgcatttg 
      ccagaggtg tcgcaagac gcccaacca ccgaaaggc 
      ttccgtgac atttacaaa cagcaaggt cccgaagct 
      tttgctaaa gccgttcgc aatcacaaa ggccttctt 
      ttgatggac acaacatat cgtgatgcc caccaatca 
      ctgctcgct accagagtc aggtcgcac gacctattg 
      cgaatttca cctttcgtt acacacaac tttaaccag 
      ttgtattcg ctggaaaat tggggcggg gctacgttt 
      gatgtcgct ttgagattc ttgcacgaa tgtccgtgg 
      gaacgcttg gaggagatg cgaaaattg atcccgaac 
      attccgttc cagatgttg ctcagagga gcaaacgct 
      gtcggctac acgaattat cccgataac gttgtcttc 
      aaattctgc gagttggcg gtccaaact ggaatggac 
      gttttccgc gtctttgac tctctgaac taccttcca 
      aacctgatt gtgggtatg gaagccgca ggcaaagct 
      ggcggaatc gtcgaagcc gctatttcc tattccggt 
      gacgtgagc aatccgaac aaaacgaaa tacgaccta 
      aaatattac ttaaacatt gctgaggaa ctagtcaag 
      gctggaacg catgttttg gccattaag gacatggcc 
      gggttactt aaaccggca gctgggagg atgcttgtg 
      agcgcttta cgtgacaaa taccccgat cttccaatc 
      catattcac acccatgac acaagtggt gctggagtt 
      gccgcaatg ttggcctgt gctgaagct ggagctgat 
      gttgttgat gttgctgtt gattccatg tctgggtta 
      acaagtcag ccaagtatg ggcgcagtt gttgcaagt 
      cttcaaggc actcaactc gacacaaaa cttgattta 
      tcgaaagtg tccgaatat tccgcctat tgggaacaa 
      accaggact ttgtatgcg ccgttcgag tgcacgacg 
      acgatgaag tcaggaaac gccgatgtt tatattaat 
      gaaattccc ggagggcag tacacgaac ttacagttc 
      caagcgtat tctctcggt cttggcgac tttttcgaa 
      gacgttaag aaggcgtac gctgaggct aacattctc 
      ctgggtgac ataataaaa gtgacacct tcgtcaaaa 
      gtcgtgggt gaccttgcc cagttcatg gtccaaaac 
      aaactgagt cctgaggac gtcctaaac aaggctgaa 
      gaattatct ttccctaaa tcagtagtg gagtatttg 
      caaggtcac atcgggcaa ccctacggc ggtttccct 
      gaaccgtta cgctcgaaa gtacttaga gacatgcca 
      cgtattgaa gggcgtcct ggggcgtcc cttcctccc 
      ttagaattc gacaaactc aagactgat cttaaggaa 
      actttcccg catgcaacc gatcgtgac gtcatgtcg 
      gcagctttg tacccccaa gtcaccaac gaatactta 
      gcgtttaaa gagcaatac ggccctgtt gataaactc 
      gacacgagg attttcctc gttgggcca aaagtgggc 
      gaagagttt gaagtgaca atcgaaaag ggcaaaact 
      ttgggcatt aaaacacta gcaatggcc gaggatttg 
      acggagaat ggggaacgt gaagtgttc tttgaactg 
      aacgggacg ctacgttcg gttcttatc agagataag 
      gatgcaggg aaagaaatc cacgtgcat cccaaagct 
      gataaagcc aataagaag caagtgggg gctccaatg 
      ccgggaact gttattgat attagggtt aaagttggg 
      gagaaagtt gagaaagga actccgttg gttattttg 
      tcggctatg aagatggaa actgtggtt cagagtccg 
      attgctggt gttatcaaa actttggat gtcaatatg 
      gggatgaaa ctggaagct gaggatttg atgatgact 
      gttgagtaa
    mRNA sequence
    • >TC032730 RA
      ttcatttga tggtgactt tgctttcta tcttcattt 
      aaaaatatt ggtcaggtc gcttacacc ggtctttct 
      caccagcag cagcggcgt ggagtgcga cattttgcg 
      gcctgaatt ttagtgaga acgactgtg atttcttca 
      attaaagta cccaacggc ctcccgcaa tgctcgtcc 
      gcctcatca gctcgaaaa ccctcctcc gctcgaccc 
      agcacatct ccttcctcc ctgcaacat gcaacttct 
      ccacaaaaa tcgaataca aaccaatca gaagcgtcc 
      tcgtggcaa atcgtggcg aaatcgccg ttcgtgttt 
      tccgcgctt gtaacgaac ttggaatca aatcggtgg 
      caatctact ccaaagagg acagagccc acattcaca 
      gactcaaag ccgatgaaa gttacctag tgggtgaag 
      gcctaccac ctgtacaag cctacctta acatacccg 
      aaattatca aaatttgta aagacaacg gcattgacg 
      ctgtacatc caggctatg gatttctgt cagaaaggg 
      ccgactttg cacaagctg ttatcgatg ccggaatta 
      gattcatcg gaccgtcgc caaaggtcg tgcaccaga 
      tgggggaca aagtggccg ccagagagg cggctattg 
      ctgcaggtg ttcctattg ttccgggga cagatggac 
      ctgtcacca caaaggagg aagccaagg agttttgcg 
      tcaaacacg gattacctg tcattttta aggcggctt 
      atgggggcg gcggccgtg gcatgcgcg tcgtccgca 
      agatggaag aagtggagg agaacttcc agagagcat 
      cgtccgaag ccctaagtg ccttcggta acggtgcca 
      tgtttattg aaaaattca tcgaacgtc caagacaca 
      tcgaagtgc aactaatgg gtgataagg ccggtaatg 
      tggtgcacc tctacgagc gcgattgtt ccgtccaac 
      ggaggcacc agaaggtgg tggaaatgg ccccagccc 
      cccatttgg acatcaacg tccgtaata aaatgaccg 
      atttggccg taaaacttg ccaaacatg tgggctatg 
      aaaatgccg gcactgttg aatttttat gcgatgaaa 
      aaggcaatt tctacttta tcgaagtga acgctcgac 
      tccaagtcg aacacacgg tcacggagg aaatcaccg 
      gaattgatt tggtgcaaa cgcaaatcc gggtcgctg 
      aaggaatga cgcttccgg agatgggaa tcgagcaag 
      aaaatatca aaccaaacg gttttgcga ttcaatgcc 
      gggtgacca ccgaagacc cagccaaaa acttccaac 
      cggatacgg gaaggatcg aagtgttcc ggtccggtg 
      aagggatgg ggatacgtc tagatggcg cgtcggcgt 
      ttgcaggag caattattt cgccctatt atgattcgc 
      tcctagtca aggtcattg cacattcga gggacttgc 
      aagcgtcgt gcgctaaaa tgaaccgag cattgaggg 
      aatttcgcg ttcgaggtg ttaaaacta acgtccctt 
      ttcttctaa acgtgttgg aaaatcaaa agtttttga 
      atggagctg tcgatactt atttcattg acgaaaatc 
      cgcaacttt ttaatttcc cacagacgc aaaatcggg 
      cacaaaaat tgctcaatt atttagggc aggtgcttg 
      ttaatggac cacaaactc ccctagcta cgtcaagta 
      aaccgtcgg atattaaac cgcatttgc cagaggtgt 
      cgcaagacg cccaaccac cgaaaggct tccgtgaca 
      tttacaaac agcaaggtc ccgaagctt ttgctaaag 
      ccgttcgca atcacaaag gccttcttt tgatggaca 
      caacatatc gtgatgccc accaatcac tgctcgcta 
      ccagagtca ggtcgcacg acctattgc gaatttcac 
      ctttcgtta cacacaact ttaaccagt tgtattcgc 
      tggaaaatt ggggcgggg ctacgtttg atgtcgctt 
      tgagattct tgcacgaat gtccgtggg aacgcttgg 
      aggagatgc gaaaattga tcccgaaca ttccgttcc 
      agatgttgc tcagaggag caaacgctg tcggctaca 
      cgaattatc ccgataacg ttgtcttca aattctgcg 
      agttggcgg tccaaactg gaatggacg ttttccgcg 
      tctttgact ctctgaact accttccaa acctgattg 
      tgggtatgg aagccgcag gcaaagctg gcggaatcg 
      tcgaagccg ctatttcct attccggtg acgtgagca 
      atccgaaca aaacgaaat acgacctaa aatattact 
      taaacattg ctgaggaac tagtcaagg ctggaacgc 
      atgttttgg ccattaagg acatggccg ggttactta 
      aaccggcag ctgggagga tgcttgtga gcgctttac 
      gtgacaaat accccgatc ttccaatcc atattcaca 
      cccatgaca caagtggtg ctggagttg ccgcaatgt 
      tggcctgtg ctgaagctg gagctgatg ttgttgatg 
      ttgctgttg attccatgt ctgggttaa caagtcagc 
      caagtatgg gcgcagttg ttgcaagtc ttcaaggca 
      ctcaactcg acacaaaac ttgatttat cgaaagtgt 
      ccgaatatt ccgcctatt gggaacaaa ccaggactt 
      tgtatgcgc cgttcgagt gcacgacga cgatgaagt 
      caggaaacg ccgatgttt atattaatg aaattcccg 
      gagggcagt acacgaact tacagttcc aagcgtatt 
      ctctcggtc ttggcgact ttttcgaag acgttaaga 
      aggcgtacg ctgaggcta acattctcc tgggtgaca 
      taataaaag tgacacctt cgtcaaaag tcgtgggtg 
      accttgccc agttcatgg tccaaaaca aactgagtc 
      ctgaggacg tcctaaaca aggctgaag aattatctt 
      tccctaaat cagtagtgg agtatttgc aaggtcaca 
      tcgggcaac cctacggcg gtttccctg aaccgttac 
      gctcgaaag tacttagag acatgccac gtattgaag 
      ggcgtcctg gggcgtccc ttcctccct tagaattcg 
      acaaactca agactgatc ttaaggaaa ctttcccgc 
      atgcaaccg atcgtgacg tcatgtcgg cagctttgt 
      acccccaag tcaccaacg aatacttag cgtttaaag 
      agcaatacg gccctgttg ataaactcg acacgagga 
      ttttcctcg ttgggccaa aagtgggcg aagagtttg 
      aagtgacaa tcgaaaagg gcaaaactt tgggcatta 
      aaacactag caatggccg aggatttga cggagaatg 
      gggaacgtg aagtgttct ttgaactga acgggacgc 
      tacgttcgg ttcttatca gagataagg atgcaggga 
      aagaaatcc acgtgcatc ccaaagctg ataaagcca 
      ataagaagc aagtggggg ctccaatgc cgggaactg 
      ttattgata ttagggtta aagttgggg agaaagttg 
      agaaaggaa ctccgttgg ttattttgt cggctatga 
      agatggaaa ctgtggttc agagtccga ttgctggtg 
      ttatcaaaa ctttggatg tcaatatgg ggatgaaac 
      tggaagctg aggatttga tgatgactg ttgagtaat 
      tgggcactt taccgtgtg ttaggctgt gttatgtca 
      tgtacaaaa aacaaacat atttttatt acattggtt 
      gagccttaa ctgtttcct ataatgaat tatttattg 
      ttatacaat tcgattttg ttgatcttg ttattttgt 
      tatctccac tttattgaa ataaagact attttttac 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY