| General information | |||
| TC007693 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 706815..715069 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007693 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04365 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
Fertility
egg production: number no/almost no eggs -
tropharium size decreased - Number of animals showing the phenotype: 4
egg chamber not present - Number of animals showing the phenotype: 4
vitellarium not present - Number of animals showing the phenotype: 4
tropharium potentially not present - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
tropharium size decreased - Number of animals showing the phenotype: 4
egg chamber not present - Number of animals showing the phenotype: 4
vitellarium not present - Number of animals showing the phenotype: 4
tropharium potentially not present - Number of animals showing the phenotype: 4
fertility after larval injection decreased (no living offspring) -
- >TC007693 RA
MALGAQSVA AIFVCSSPL IWCCCSYTT QGIKFSLRP KMNASEEFP ESGKEPAEK VEEFGELVM FEGNGKLCP SEALEDGLL ADPDKCGMC GGSLVVSRM LHCLHSFCE DCLDKKMVG EGGDAGTAE ATIECPTCG HHTKVGVKK MAALPLDVT KTNISDVSN ASSLHCTSC TAKELAKSR CNTCHNLLC NHCDSAHHY MRCFESHQV VALEDMRKD GVKITIHKP LECDLHQGE NLIYYCNTC NTTACSECV KNEHKGAEH HVEGIADSE MRVRQEMKA LLSESKNKV DELMKLSTG LNNSLEELA HQRSTARDL INESYQSYK AVLEKCRDE ALAKLNELY HERELIIMK ENDEVGKTI GNFEDARSY TSRLLEIST VPEIMYLRK TVADRLLEL NNNTPKCDE TFHIEFRTD FGAFESMVK EHFGDFRTE KSRVSSPIN SALTPLTIT QPLTNGCNA SITNSSPIS LPTSMQSSF DGDVSGNMQ NFALAQSPP LPQLATLAE TSNSAATSP TPPFNLADL LTSDTAYKN LASLAKLGL NADNPITNG TMIVRSTSP ASLNLTNSL INGFSGVSS STSPLLSTP DDIMSDPMS NILPAPTPN PNGTHTTRS NKVSPMQIR SKFGQLGPN KGQFNSPHG FCLGLEEDI IVADTNNHR IQVFDKSGV FKFQFGTSG KDEGQLWYP RKVAVMRTT GKYVVCDRG NERSRMQIF TKNGHFLKK IAIRYIDIV AGLAVTGHG EIVAVDSVS PTVFIISES GELIRWFDC SDFMREPSD IAIHGKEFY VCDFKGHNV VVFSDEGHF LRRIGYESI TNFPNGIDI SDAGDILIG DSHGNRFHV AVFSRDGCL ISEFECPYV KVSRCCGLK ITSEGYVSP KMSSRSS
- >TC007693 RB
MALGAQSVA AIFVCSSPL IWCCCSYTT QGIKFSLRP KMNASEEFP ESGKEPAEK VEEFGELVM FEGNGKLCP SEALEDGLL ADPDKCGMC GGSLVVSRM LHCLHSFCE DCLDKKMVG EGGDAGTAE ATIECPTCG HHTKVGVKK MAALPLDVT KTNISDVSN ASSLHCTSC TAKELAKSR CNTCHNLLC NHCDSAHHY MRCFESHQV VALEDMRKD GVKITIHKP LECDLHQGE NLIYYCNTC NTTACSECV KNEHKGAEH HVEGIADSE MRVRQEMKA LLSESKNKV DELMKLSTG LNNSLEELA HQRSTARDL INESYQSYK AVLEKCRDE ALAKLNELY HERELIIMK ENDEVGKTI GNFEDARSY TSRLLEIST VPEIMYLRK TVADRLLEL NNNTPKCDE TFHIEFRTD FGAFESMVK EHFGDFRTE KSRVSSPIN SALTPLTIT QPLTNGCNA SITNSSPIS LPTSMQSSF DGDVSGNMQ NFALAQSPP LPQLATLAE TSNSAATSP TPPFNLADL LTSDTAYKN LASLAKLGL NAAPTPNPN GTHTTRSNK VSPMQIRSK FGQLGPNKG QFNSPHGFC LGLEEDIIV ADTNNHRIQ VFDKSGVFK FQFGTSGKD EGQLWYPRK VAVMRTTGK YVVCDRGNE RSRMQIFTK NGHFLKKIA IRYIDIVAG LAVTGHGEI VAVDSVSPT VFIISESGE LIRWFDCSD FMREPSDIA IHGKEFYVC DFKGHNVVV FSDEGHFLR RIGYESITN FPNGIDISD AGDILIGDS HGNRFHVAV FSRDGCLIS EFECPYVKV SRCCGLKIT SEGYVSPKM SSRSS
- >TC007693 RA
atggcattg ggggcgcaa agcgtcgcc gccatcttt gtttgcagc agccccttg atttggtgt tgttgcagt tacactacc caagggatt aagttttcg ttaaggcct aaaatgaac gcttcggag gaatttcct gagtcgggt aaggaacca gccgagaaa gtggaggag tttggggag ctggtgatg tttgaaggg aatggcaaa ttgtgccct tcggaggcc ttggaggac ggcttattg gccgatccg gacaagtgt ggcatgtgt gggggatca cttgtggtg tctcgaatg ctgcactgt ttgcattcg ttttgcgaa gattgtctc gataagaaa atggttggg gaaggaggt gacgctggg acggctgag gctactatt gagtgtccc acttgtgga catcacacc aaggtcggg gtgaaaaaa atggctgct cttcctctt gatgtgacc aaaaccaac atctctgac gtgtcaaat gcgtccagt ttgcattgc acctcttgt accgccaaa gaattggcc aaatcacgt tgcaacacc tgccataat ctcttgtgt aaccattgt gattcggcg catcattac atgcgttgt ttcgagtcg catcaagtc gtagccttg gaagatatg cgtaaagac ggagtcaaa ataaccatt cataagccg ctcgaatgc gaccttcac caaggcgaa aatttgatc tactattgc aacacttgt aatacgacg gcttgtagc gaatgcgtt aaaaacgaa cacaaaggt gccgagcat catgtcgaa ggcatagcc gattcggaa atgagagtt agacaagag atgaaggca ctattgtcc gaaagtaag aacaaagtc gatgagttg atgaaactc tcgactgga ttgaataac agtttggaa gagttggca catcaacgg tctacagca cgtgacctc atcaacgaa tcgtatcag agttacaaa gcggttttg gaaaaatgt cgagacgaa gctttggcc aaactgaac gaactttat cacgagcgg gagttgata ataatgaag gaaaatgac gaagttgga aaaactatc ggtaatttc gaggatgca agatcttac acttcgcgt cttttggaa atatctacc gttcctgaa ataatgtac ctgagaaaa acagttgct gatcgttta ttggaactc aacaacaac acaccgaaa tgcgacgag acatttcac attgaattt cgcacagac tttggggct ttcgaatcg atggtcaag gaacatttt ggtgatttt cgtacggaa aaatcgcgt gtttcaagt ccgataaat tcggctcta acaccgctg actattaca caaccgctc accaatggt tgtaatgcg tcgattacg aatagtagt ccgatttcg ttgccgact tcgatgcag tcgtcgttt gatggagac gtcagtgga aatatgcag aatttcgct ttggcgcag agtccacct ttgcctcag ttggcgact ttggctgag acttcaaat agtgcagcg acttcaccg acaccacct tttaatttg gccgattta ctcaccagc gacactgcc tataagaat cttgcttcg cttgccaag ctcggactg aatgcggat aatccgata accaacggt acaatgata gtccgttca acgtcgccc gcatctctc aacttgacc aacagctta atcaacggt ttcagtgga gtttcctcc tccacatct cctttactc tccactcct gacgacata atgtctgat cccatgtca aatattctt ccagcacct actcctaat ccaaatggt acccacacc actcgttca aacaaagtc agtcctatg caaattcgt agcaagttt ggccaactc ggtcctaac aaaggccag tttaattca ccccacgga ttctgcctc ggtctagaa gaggacatt attgttgct gatacgaat aaccatcgc attcaagta tttgataag tcgggggtt ttcaaattc caatttgga acctcaggt aaggacgaa gggcagttg tggtatcca cgtaaagtg gcagtgatg cgcaccacc ggcaagtac gtcgtatgc gatcgcgga aatgaacgc tcacgcatg caaatcttc acgaaaaac ggtcatttc ttgaagaag attgccatc agatacatc gatatcgta gccggactt gcagtcact ggacatgga gagattgtg gctgttgat agtgtcagt ccgactgta tttattatc agcgaaagt ggggagttg attcgttgg ttcgattgc agcgacttt atgagagaa ccttccgat attgccatt cacgggaaa gaattctac gtttgtgac tttaagggt cacaacgta gttgttttc agcgatgaa gggcatttt ttgagacga attggttac gaaagcatt actaatttt ccaaacggg attgatatc tccgacgct ggagatatc ctgattggg gattcgcac ggaaatcgt tttcatgtt gctgtgttt tcgcgtgat gggtgtctc atttcggaa tttgaatgc ccctatgtt aaggtgtct cgttgctgc ggtttgaag atcacctcc gagggttat gtcagcccc aaaatgtcg tcgagatct tcgtga
- >TC007693 RB
atggcattg ggggcgcaa agcgtcgcc gccatcttt gtttgcagc agccccttg atttggtgt tgttgcagt tacactacc caagggatt aagttttcg ttaaggcct aaaatgaac gcttcggag gaatttcct gagtcgggt aaggaacca gccgagaaa gtggaggag tttggggag ctggtgatg tttgaaggg aatggcaaa ttgtgccct tcggaggcc ttggaggac ggcttattg gccgatccg gacaagtgt ggcatgtgt gggggatca cttgtggtg tctcgaatg ctgcactgt ttgcattcg ttttgcgaa gattgtctc gataagaaa atggttggg gaaggaggt gacgctggg acggctgag gctactatt gagtgtccc acttgtgga catcacacc aaggtcggg gtgaaaaaa atggctgct cttcctctt gatgtgacc aaaaccaac atctctgac gtgtcaaat gcgtccagt ttgcattgc acctcttgt accgccaaa gaattggcc aaatcacgt tgcaacacc tgccataat ctcttgtgt aaccattgt gattcggcg catcattac atgcgttgt ttcgagtcg catcaagtc gtagccttg gaagatatg cgtaaagac ggagtcaaa ataaccatt cataagccg ctcgaatgc gaccttcac caaggcgaa aatttgatc tactattgc aacacttgt aatacgacg gcttgtagc gaatgcgtt aaaaacgaa cacaaaggt gccgagcat catgtcgaa ggcatagcc gattcggaa atgagagtt agacaagag atgaaggca ctattgtcc gaaagtaag aacaaagtc gatgagttg atgaaactc tcgactgga ttgaataac agtttggaa gagttggca catcaacgg tctacagca cgtgacctc atcaacgaa tcgtatcag agttacaaa gcggttttg gaaaaatgt cgagacgaa gctttggcc aaactgaac gaactttat cacgagcgg gagttgata ataatgaag gaaaatgac gaagttgga aaaactatc ggtaatttc gaggatgca agatcttac acttcgcgt cttttggaa atatctacc gttcctgaa ataatgtac ctgagaaaa acagttgct gatcgttta ttggaactc aacaacaac acaccgaaa tgcgacgag acatttcac attgaattt cgcacagac tttggggct ttcgaatcg atggtcaag gaacatttt ggtgatttt cgtacggaa aaatcgcgt gtttcaagt ccgataaat tcggctcta acaccgctg actattaca caaccgctc accaatggt tgtaatgcg tcgattacg aatagtagt ccgatttcg ttgccgact tcgatgcag tcgtcgttt gatggagac gtcagtgga aatatgcag aatttcgct ttggcgcag agtccacct ttgcctcag ttggcgact ttggctgag acttcaaat agtgcagcg acttcaccg acaccacct tttaatttg gccgattta ctcaccagc gacactgcc tataagaat cttgcttcg cttgccaag ctcggactg aatgcggca cctactcct aatccaaat ggtacccac accactcgt tcaaacaaa gtcagtcct atgcaaatt cgtagcaag tttggccaa ctcggtcct aacaaaggc cagtttaat tcaccccac ggattctgc ctcggtcta gaagaggac attattgtt gctgatacg aataaccat cgcattcaa gtatttgat aagtcgggg gttttcaaa ttccaattt ggaacctca ggtaaggac gaagggcag ttgtggtat ccacgtaaa gtggcagtg atgcgcacc accggcaag tacgtcgta tgcgatcgc ggaaatgaa cgctcacgc atgcaaatc ttcacgaaa aacggtcat ttcttgaag aagattgcc atcagatac atcgatatc gtagccgga cttgcagtc actggacat ggagagatt gtggctgtt gatagtgtc agtccgact gtatttatt atcagcgaa agtggggag ttgattcgt tggttcgat tgcagcgac tttatgaga gaaccttcc gatattgcc attcacggg aaagaattc tacgtttgt gactttaag ggtcacaac gtagttgtt ttcagcgat gaagggcat tttttgaga cgaattggt tacgaaagc attactaat tttccaaac gggattgat atctccgac gctggagat atcctgatt ggggattcg cacggaaat cgttttcat gttgctgtg ttttcgcgt gatgggtgt ctcatttcg gaatttgaa tgcccctat gttaaggtg tctcgttgc tgcggtttg aagatcacc tccgagggt tatgtcagc cccaaaatg tcgtcgaga tcttcgtga
- >TC007693 RA
tatttgaca catccccac tcaataacc cattgccct tgcgctaaa gacgcaaga cgcgccatt tgctaaata aacacttgc tcgttatgg cattggggg cgcaaagcg tcgccgcca tctttgttt gcagcagcc ccttgattt ggtgttgtt gcagttaca ctacccaag ggattaagt tttcgttaa ggcctaaaa tgaacgctt cggaggaat ttcctgagt cgggtaagg aaccagccg agaaagtgg aggagtttg gggagctgg tgatgtttg aagggaatg gcaaattgt gcccttcgg aggccttgg aggacggct tattggccg atccggaca agtgtggca tgtgtgggg gatcacttg tggtgtctc gaatgctgc actgtttgc attcgtttt gcgaagatt gtctcgata agaaaatgg ttggggaag gaggtgacg ctgggacgg ctgaggcta ctattgagt gtcccactt gtggacatc acaccaagg tcggggtga aaaaaatgg ctgctcttc ctcttgatg tgaccaaaa ccaacatct ctgacgtgt caaatgcgt ccagtttgc attgcacct cttgtaccg ccaaagaat tggccaaat cacgttgca acacctgcc ataatctct tgtgtaacc attgtgatt cggcgcatc attacatgc gttgtttcg agtcgcatc aagtcgtag ccttggaag atatgcgta aagacggag tcaaaataa ccattcata agccgctcg aatgcgacc ttcaccaag gcgaaaatt tgatctact attgcaaca cttgtaata cgacggctt gtagcgaat gcgttaaaa acgaacaca aaggtgccg agcatcatg tcgaaggca tagccgatt cggaaatga gagttagac aagagatga aggcactat tgtccgaaa gtaagaaca aagtcgatg agttgatga aactctcga ctggattga ataacagtt tggaagagt tggcacatc aacggtcta cagcacgtg acctcatca acgaatcgt atcagagtt acaaagcgg ttttggaaa aatgtcgag acgaagctt tggccaaac tgaacgaac tttatcacg agcgggagt tgataataa tgaaggaaa atgacgaag ttggaaaaa ctatcggta atttcgagg atgcaagat cttacactt cgcgtcttt tggaaatat ctaccgttc ctgaaataa tgtacctga gaaaaacag ttgctgatc gtttattgg aactcaaca acaacacac cgaaatgcg acgagacat ttcacattg aatttcgca cagactttg gggctttcg aatcgatgg tcaaggaac attttggtg attttcgta cggaaaaat cgcgtgttt caagtccga taaattcgg ctctaacac cgctgacta ttacacaac cgctcacca atggttgta atgcgtcga ttacgaata gtagtccga tttcgttgc cgacttcga tgcagtcgt cgtttgatg gagacgtca gtggaaata tgcagaatt tcgctttgg cgcagagtc cacctttgc ctcagttgg cgactttgg ctgagactt caaatagtg cagcgactt caccgacac cacctttta atttggccg atttactca ccagcgaca ctgcctata agaatcttg cttcgcttg ccaagctcg gactgaatg cggataatc cgataacca acggtacaa tgatagtcc gttcaacgt cgcccgcat ctctcaact tgaccaaca gcttaatca acggtttca gtggagttt cctcctcca catctcctt tactctcca ctcctgacg acataatgt ctgatccca tgtcaaata ttcttccag cacctactc ctaatccaa atggtaccc acaccactc gttcaaaca aagtcagtc ctatgcaaa ttcgtagca agtttggcc aactcggtc ctaacaaag gccagttta attcacccc acggattct gcctcggtc tagaagagg acattattg ttgctgata cgaataacc atcgcattc aagtatttg ataagtcgg gggttttca aattccaat ttggaacct caggtaagg acgaagggc agttgtggt atccacgta aagtggcag tgatgcgca ccaccggca agtacgtcg tatgcgatc gcggaaatg aacgctcac gcatgcaaa tcttcacga aaaacggtc atttcttga agaagattg ccatcagat acatcgata tcgtagccg gacttgcag tcactggac atggagaga ttgtggctg ttgatagtg tcagtccga ctgtattta ttatcagcg aaagtgggg agttgattc gttggttcg attgcagcg actttatga gagaacctt ccgatattg ccattcacg ggaaagaat tctacgttt gtgacttta agggtcaca acgtagttg ttttcagcg atgaagggc attttttga gacgaattg gttacgaaa gcattacta attttccaa acgggattg atatctccg acgctggag atatcctga ttggggatt cgcacggaa atcgttttc atgttgctg tgttttcgc gtgatgggt gtctcattt cggaatttg aatgcccct atgttaagg tgtctcgtt gctgcggtt tgaagatca cctccgagg gttatgtca gccccaaaa tgtcgtcga gatcttcgt gatgtttgg ttggtttga taaaatttt gagcaaaat tgataaagc agcagttct ctattacct gcctcgttt ttaataaat taaacaaat cagttgagc tgtggcatt taagaagtt tttgataga actattttt gatgtgaac ggatcgcac agcgtgtgt gacacgctt gtgcggttc gtatattct gtaacaatc aactgtcga aaaaataca aaaaaatac ggccgattt caagcggtc tatgtgtac ctattacct atattgtaa ttaatagca ctcttgttt ctctgctta accttccac tatgataat aaaacaaag ttaaatact gtcgtaaat tcagagtag cacatcgta aaatattaa agagtaata atattgctt tgtctcgtt tacaatcga tacttatac cgcgtatac actttatct tattattgt aatgttata tgatgtgtg ttatattcc aagtcgttt atggacatt cgatataga atcttttcc attattcta aaattcatg cctctgaac agtctgtac taaaattta tattattat atttgttgc actctatgg ttattcagg gttacaaga gctatgtga gctcgtatt tttggaact gttttttat ttaaattgt actgaggtt gtttttaat ttgtaagtt atattattg gtttttgaa taaatataa tccaaaaaa
- >TC007693 RB
tatttgaca catccccac tcaataacc cattgccct tgcgctaaa gacgcaaga cgcgccatt tgctaaata aacacttgc tcgttatgg cattggggg cgcaaagcg tcgccgcca tctttgttt gcagcagcc ccttgattt ggtgttgtt gcagttaca ctacccaag ggattaagt tttcgttaa ggcctaaaa tgaacgctt cggaggaat ttcctgagt cgggtaagg aaccagccg agaaagtgg aggagtttg gggagctgg tgatgtttg aagggaatg gcaaattgt gcccttcgg aggccttgg aggacggct tattggccg atccggaca agtgtggca tgtgtgggg gatcacttg tggtgtctc gaatgctgc actgtttgc attcgtttt gcgaagatt gtctcgata agaaaatgg ttggggaag gaggtgacg ctgggacgg ctgaggcta ctattgagt gtcccactt gtggacatc acaccaagg tcggggtga aaaaaatgg ctgctcttc ctcttgatg tgaccaaaa ccaacatct ctgacgtgt caaatgcgt ccagtttgc attgcacct cttgtaccg ccaaagaat tggccaaat cacgttgca acacctgcc ataatctct tgtgtaacc attgtgatt cggcgcatc attacatgc gttgtttcg agtcgcatc aagtcgtag ccttggaag atatgcgta aagacggag tcaaaataa ccattcata agccgctcg aatgcgacc ttcaccaag gcgaaaatt tgatctact attgcaaca cttgtaata cgacggctt gtagcgaat gcgttaaaa acgaacaca aaggtgccg agcatcatg tcgaaggca tagccgatt cggaaatga gagttagac aagagatga aggcactat tgtccgaaa gtaagaaca aagtcgatg agttgatga aactctcga ctggattga ataacagtt tggaagagt tggcacatc aacggtcta cagcacgtg acctcatca acgaatcgt atcagagtt acaaagcgg ttttggaaa aatgtcgag acgaagctt tggccaaac tgaacgaac tttatcacg agcgggagt tgataataa tgaaggaaa atgacgaag ttggaaaaa ctatcggta atttcgagg atgcaagat cttacactt cgcgtcttt tggaaatat ctaccgttc ctgaaataa tgtacctga gaaaaacag ttgctgatc gtttattgg aactcaaca acaacacac cgaaatgcg acgagacat ttcacattg aatttcgca cagactttg gggctttcg aatcgatgg tcaaggaac attttggtg attttcgta cggaaaaat cgcgtgttt caagtccga taaattcgg ctctaacac cgctgacta ttacacaac cgctcacca atggttgta atgcgtcga ttacgaata gtagtccga tttcgttgc cgacttcga tgcagtcgt cgtttgatg gagacgtca gtggaaata tgcagaatt tcgctttgg cgcagagtc cacctttgc ctcagttgg cgactttgg ctgagactt caaatagtg cagcgactt caccgacac cacctttta atttggccg atttactca ccagcgaca ctgcctata agaatcttg cttcgcttg ccaagctcg gactgaatg cggcaccta ctcctaatc caaatggta cccacacca ctcgttcaa acaaagtca gtcctatgc aaattcgta gcaagtttg gccaactcg gtcctaaca aaggccagt ttaattcac cccacggat tctgcctcg gtctagaag aggacatta ttgttgctg atacgaata accatcgca ttcaagtat ttgataagt cgggggttt tcaaattcc aatttggaa cctcaggta aggacgaag ggcagttgt ggtatccac gtaaagtgg cagtgatgc gcaccaccg gcaagtacg tcgtatgcg atcgcggaa atgaacgct cacgcatgc aaatcttca cgaaaaacg gtcatttct tgaagaaga ttgccatca gatacatcg atatcgtag ccggacttg cagtcactg gacatggag agattgtgg ctgttgata gtgtcagtc cgactgtat ttattatca gcgaaagtg gggagttga ttcgttggt tcgattgca gcgacttta tgagagaac cttccgata ttgccattc acgggaaag aattctacg tttgtgact ttaagggtc acaacgtag ttgttttca gcgatgaag ggcattttt tgagacgaa ttggttacg aaagcatta ctaattttc caaacggga ttgatatct ccgacgctg gagatatcc tgattgggg attcgcacg gaaatcgtt ttcatgttg ctgtgtttt cgcgtgatg ggtgtctca tttcggaat ttgaatgcc cctatgtta aggtgtctc gttgctgcg gtttgaaga tcacctccg agggttatg tcagcccca aaatgtcgt cgagatctt cgtgatgtt tggttggtt tgataaaat tttgagcaa aattgataa agcagcagt tctctatta cctgcctcg tttttaata aattaaaca aatcagttg agctgtggc atttaagaa gtttttgat agaactatt tttgatgtg aacggatcg cacagcgtg tgtgacacg cttgtgcgg ttcgtatat tctgtaaca atcaactgt cgaaaaaat acaaaaaaa tacggccga tttcaagcg gtctatgtg tacctatta cctatattg taattaata gcactcttg tttctctgc ttaaccttc cactatgat aataaaaca aagttaaat actgtcgta aattcagag tagcacatc gtaaaatat taaagagta ataatattg ctttgtctc gtttacaat cgatactta taccgcgta tacacttta tcttattat tgtaatgtt atatgatgt gtgttatat tccaagtcg tttatggac attcgatat agaatcttt tccattatt ctaaaattc atgcctctg aacagtctg tactaaaat ttatattat tatatttgt tgcactcta tggttattc agggttaca agagctatg tgagctcgt atttttgga actgttttt tatttaaat tgtactgag gttgttttt aatttgtaa gttatatta ttggttttt gaataaata taatccaaa aaa
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