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Gene information
General information
TC007687 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 899437..902531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007687
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04364

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007687 RA
      MDEDTTRSQ FSELVRYSR AMLTAAGIV TLVIMVVGV 
IGNLLTVFA LMRNSKIRT VAAAFIASL CISDLMFCF 
IVLPFAASQ FFHGTWIHG DVLCTIIPV LRYGCVGVS 
LLSIAMISV NRYILIAWP HVYGQIYTK AKVAIYITA 
IWLFSYGLQ IPTLLGQWG LYGFDKRLG TCSINKDAN 
GRSSKTALF VIGFALPCA VIVVCYANI FWVVRKSHK 
RLKQHNSGG DYKRSEMKI TKMVLVIFV CFVVCYLPI 
TIVKIFDPE VKQAPLHVL GYILIYISS CVNPVVYVT 
MNKQYRQAY LDTLRCRMT SAGDSMHTP SPHSRTHLS 
VGYLKNVVN NSPKV
    CDS sequence
    • >TC007687 RA
      atggacgaa gacacgacg cggtcgcaa ttttccgag 
ctcgtccga tattcgagg gcgatgctg accgcagcc 
gggattgtc acactcgtc atcatggtg gtgggtgtg 
attgggaac ttactcacg gttttcgcg ctcatgagg 
aactccaag attcggacg gtcgccgct gcttttatt 
gcaagcctg tgcatctcc gacctgatg ttctgcttc 
atcgtcctc ccgttcgcc gcgagtcaa ttcttccac 
ggtacgtgg atccacggc gacgtcctg tgcaccata 
atccccgtt ttgcgctac ggatgcgtg ggagtctcc 
ctcctgagc atcgcaatg atatcagta aaccgctac 
atcctcatc gcttggccc cacgtctac ggccagatt 
tacaccaag gcgaaggtc gccatctac atcacggcc 
atctggctc ttcagctac ggcctccag atcccgact 
ttgctcggc caatggggg ctgtacggc ttcgataag 
cgactgggc acctgctcg atcaacaaa gacgcaaat 
gggcgctcg tccaaaacg gcgcttttt gtgatcggg 
tttgcgctc ccatgtgct gttatcgtg gtttgctat 
gcgaatatt ttctgggtg gtgaggaag agtcataag 
aggctcaag cagcataat tcagggggc gattacaag 
cgcagcgaa atgaaaata acgaagatg gtgctggtg 
atttttgtg tgttttgtt gtgtgttat ctgccgatt 
actatcgtg aagattttt gatccggaa gtgaaacaa 
gcaccgttg catgtcctg ggctacatc ctaatctac 
atttcctct tgtgtgaat ccagtggtg tacgttaca 
atgaataaa cagtacaga caggcctat ttggacact 
ttaaggtgt cgcatgaca agtgctggg gactccatg 
catacgccg agcccccac agtcggacg cacctttcg 
gtggggtac ctcaaaaac gtggtaaac aactcgccg 
aaagtgtga
    mRNA sequence
    • >TC007687 RA
      tgattagtc ccaattaaa attaatttt taaccatcc 
tgtgtttgt gcacgaggg agcgccccc ttaatcagc 
catggacga agacacgac gcggtcgca attttccga 
gctcgtccg atattcgag ggcgatgct gaccgcagc 
cgggattgt cacactcgt catcatggt ggtgggtgt 
gattgggaa cttactcac ggttttcgc gctcatgag 
gaactccaa gattcggac ggtcgccgc tgcttttat 
tgcaagcct gtgcatctc cgacctgat gttctgctt 
catcgtcct cccgttcgc cgcgagtca attcttcca 
cggtacgtg gatccacgg cgacgtcct gtgcaccat 
aatccccgt tttgcgcta cggatgcgt gggagtctc 
cctcctgag catcgcaat gatatcagt aaaccgcta 
catcctcat cgcttggcc ccacgtcta cggccagat 
ttacaccaa ggcgaaggt cgccatcta catcacggc 
catctggct cttcagcta cggcctcca gatcccgac 
tttgctcgg ccaatgggg gctgtacgg cttcgataa 
gcgactggg cacctgctc gatcaacaa agacgcaaa 
tgggcgctc gtccaaaac ggcgctttt tgtgatcgg 
gtttgcgct cccatgtgc tgttatcgt ggtttgcta 
tgcgaatat tttctgggt ggtgaggaa gagtcataa 
gaggctcaa gcagcataa ttcaggggg cgattacaa 
gcgcagcga aatgaaaat aacgaagat ggtgctggt 
gatttttgt gtgttttgt tgtgtgtta tctgccgat 
tactatcgt gaagatttt tgatccgga agtgaaaca 
agcaccgtt gcatgtcct gggctacat cctaatcta 
catttcctc ttgtgtgaa tccagtggt gtacgttac 
aatgaataa acagtacag acaggccta tttggacac 
tttaaggtg tcgcatgac aagtgctgg ggactccat 
gcatacgcc gagccccca cagtcggac gcacctttc 
ggtggggta cctcaaaaa cgtggtaaa caactcgcc 
gaaagtgtg atcctaata tctattccc ttaatattt 
atgtaaata ccaaagtac aaatacctt acattaagg 
ctttttatc gtgtacata gttttttat taaataaaa 
ttttatcga cgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY