Status
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Gene information
General information
TC007682 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 934021..935491
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007682
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007682 RA
      MRITFCTDL DKSVVVANC EKRGWIQVG PEDDWNIYW 
      AGTQTCRSL FSVDSGYRM HDNQLINHF PNHYEISRK 
      DLLVKNIKR YRRDLEKEG NVLAERGDS KYLHLDFIP 
      VTFVLPADY NMFVEEYRK APQSTWIMK PCGRSQGSG 
      IFLINKLSK LKRWSREAK SPFQQQLTK ESYVISKYI 
      DNPLLIGGK KFDLRLYVL VTSFRPLKA YQFRLGFCR 
      FCTVKYDSS VAELDNMYV HLTNVSVQK HGGEYNSLH 
      GGKLSVQNL RLHLESTRG KQVTERLFD QISWLIIHS 
      LKAVAPVIA TDRHCFECY GYDIIIDNK LKPWLIEVN 
      ASPSLTSTT ANDRILKHK LMDNIFSIV VPPSGLPDV 
      RWNKIPSQE ALGDFELLI DEDLIHLHE TENNSYLLK 
      NNRWK
    CDS sequence
    • >TC007682 RA
      atgcggata actttttgc accgatctc gacaaaagt 
      gtcgttgtg gcaaactgt gaaaaacgc ggttggata 
      caagtgggg cccgaagat gattggaat atttattgg 
      gcggggact cagacttgt aggagtttg tttagtgtg 
      gatagtgga taccgcatg cacgataat caattaatc 
      aatcatttc cccaatcat tacgaaatc tcccgtaaa 
      gacttgctc gtgaaaaac atcaagcgc taccgacgc 
      gatttggaa aaagaaggc aatgttttg gccgaacgt 
      ggagattca aaatacctc caccttgat tttattcct 
      gtaactttc gtgctccca gctgactat aacatgttc 
      gttgaagag tacagaaag gcgcctcag agcacttgg 
      atcatgaaa ccgtgtggc cgatcgcaa ggctccggt 
      atttttctc atcaataaa ttatcaaag ctcaaacgc 
      tggtcacgc gaggccaaa agccccttc cagcagcaa 
      ctcaccaaa gagagctac gtcatttcc aaatacatc 
      gataatcct ctcctaatc ggcggcaaa aaattcgac 
      ttgcgcctt tacgtcctt gttacgtct ttccgtccc 
      ttgaaagcg taccagttc cggttgggt ttctgccgc 
      ttttgcacc gtcaaatac gactcaagt gtggcagag 
      ctcgacaat atgtacgtc catttgacg aatgtgtcc 
      gtacaaaaa catgggggt gagtacaac tcgcttcat 
      ggtggcaag ttaagtgtg caaaacttg cggctccat 
      ttggagagc acacgaggg aagcaagtg actgagcgt 
      ctttttgac caaatcagt tggttaatc attcattca 
      ctcaaagct gtagctccg gtgattgcc accgatcga 
      cactgtttc gaatgctac ggctacgac atcatcatt 
      gacaacaag ctcaaaccg tggcttatt gaagtaaat 
      gcctcaccg tcgctaact tcaaccacg gccaatgat 
      cgtattttg aaacacaaa ctcatggat aatattttc 
      tcgattgtt gtgccccca tctgggcta cctgatgtt 
      cgttggaat aaaattccg agtcaggag gcgctaggt 
      gactttgag ctgctgata gacgaggat ttgattcat 
      ttgcacgaa actgagaat aatagttac ttgttgaag 
      aacaatagg tggaagtaa
    mRNA sequence
    • >TC007682 RA
      aaagacatc gaaaaaccg tcatccgtt tattaaatt 
      ccagtgaca atgcggata actttttgc accgatctc 
      gacaaaagt gtcgttgtg gcaaactgt gaaaaacgc 
      ggttggata caagtgggg cccgaagat gattggaat 
      atttattgg gcggggact cagacttgt aggagtttg 
      tttagtgtg gatagtgga taccgcatg cacgataat 
      caattaatc aatcatttc cccaatcat tacgaaatc 
      tcccgtaaa gacttgctc gtgaaaaac atcaagcgc 
      taccgacgc gatttggaa aaagaaggc aatgttttg 
      gccgaacgt ggagattca aaatacctc caccttgat 
      tttattcct gtaactttc gtgctccca gctgactat 
      aacatgttc gttgaagag tacagaaag gcgcctcag 
      agcacttgg atcatgaaa ccgtgtggc cgatcgcaa 
      ggctccggt atttttctc atcaataaa ttatcaaag 
      ctcaaacgc tggtcacgc gaggccaaa agccccttc 
      cagcagcaa ctcaccaaa gagagctac gtcatttcc 
      aaatacatc gataatcct ctcctaatc ggcggcaaa 
      aaattcgac ttgcgcctt tacgtcctt gttacgtct 
      ttccgtccc ttgaaagcg taccagttc cggttgggt 
      ttctgccgc ttttgcacc gtcaaatac gactcaagt 
      gtggcagag ctcgacaat atgtacgtc catttgacg 
      aatgtgtcc gtacaaaaa catgggggt gagtacaac 
      tcgcttcat ggtggcaag ttaagtgtg caaaacttg 
      cggctccat ttggagagc acacgaggg aagcaagtg 
      actgagcgt ctttttgac caaatcagt tggttaatc 
      attcattca ctcaaagct gtagctccg gtgattgcc 
      accgatcga cactgtttc gaatgctac ggctacgac 
      atcatcatt gacaacaag ctcaaaccg tggcttatt 
      gaagtaaat gcctcaccg tcgctaact tcaaccacg 
      gccaatgat cgtattttg aaacacaaa ctcatggat 
      aatattttc tcgattgtt gtgccccca tctgggcta 
      cctgatgtt cgttggaat aaaattccg agtcaggag 
      gcgctaggt gactttgag ctgctgata gacgaggat 
      ttgattcat ttgcacgaa actgagaat aatagttac 
      ttgttgaag aacaatagg tggaagtaa aggcgtctt 
      tgtgttaaa taaatatac atttattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY