Status
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Gene information
General information
TC007671 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1092174..1094695
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007671
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04362

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007671 RA
      MESNNSRKK LIKKLRKHQ HTVGKETGV VCTDSPTKN 
      LVICNTGQI NGLTEEIIV EHFEKYGHL DRVLLVPGK 
      SCAFLSYKD TSSASEALE AVSGKLNIA QDGKPIFLS 
      FVEALPEIR DAKIWGRLP PGLRIITNF VSEEEEARL 
      LALCQFEDG GSMKHRLVK HYGYEFRYD INNVDKEKP 
      LSEGIPQEC DFLWRRLPF EFRPNQLTI NRYNPGQGI 
      PSHVDTHSA FGDPILSLS LSSDVVMEF KKDETICVL 
      LPRRSLLVM AGESRYEWT HGIVPRTFD FYNDEGGCH 
      CFKRGVRVS FTFRKIRKG GCNCSYKLQ CDSQTATKI 
      IESDLLAHK LENQHVHDV YEDIAGHFS ETRHTPWPN 
      VLNFVQKLE IGAVLVDVG CGNGKYFGH NRQIVELGT 
      DRSFKLNNL CKHRGFEVF TGNCLNLPL KNSSADAVI 
      SIAVIHHLS TPERRLKAL KEIVRILRI GGEALIYVW 
      AKQQIKNDE KSTYIKQDR KNRKKEVIG SVTKEEVSV 
      GEGVTLPVH TNRANFTHS DVLVPWKLK GEEQERTFL 
      RFYHVFEEG ELESLCQSI GNVSIVKSY YDQGNYCVQ 
      FVKNNK
    CDS sequence
    • >TC007671 RA
      atggaatct aacaattcc cgcaaaaaa ctcattaaa 
      aaactgcga aagcaccaa cacactgtt gggaaagaa 
      accggagtc gtctgtacc gacagcccc acgaaaaac 
      cttgtaatt tgcaatact ggccaaatt aacggctta 
      actgaggaa ataatcgtt gaacacttc gaaaaatac 
      ggtcacctc gaccgcgtt ttactagtt cctggaaaa 
      tcgtgcgct tttttgagc tataaagac acctcaagt 
      gcttcagaa gcattggag gctgttagc ggtaaattg 
      aatatagcc caagacggc aaaccgatt tttttgagt 
      tttgttgag gctttgcct gagataagg gatgcgaaa 
      atttggggg cgcctcccg ccaggtttg aggataatt 
      acgaatttt gttagtgag gaagaagag gcaagattg 
      ttggctttg tgccagttt gaagatggc ggaagcatg 
      aaacacaga ttagtaaaa cactacggt tatgagttt 
      cgttacgat attaacaat gttgataaa gagaagccg 
      ttaagtgag ggaatcccc caagagtgc gatttttta 
      tggagacgc cttccgttt gaattccgc cccaatcag 
      ttaacaatc aatcggtac aatcccgga caggggatt 
      ccctcacac gtggacacc cacagcgcg ttcggggac 
      cccatcctg tctctaagc ctcagttct gacgttgtg 
      atggaattc aaaaaagac gaaacgatt tgtgttctt 
      ttaccccga aggtcgtta cttgtaatg gcaggggag 
      agtaggtac gaatggacg catgggatc gtgccccga 
      acttttgat ttttacaat gatgaaggg ggctgtcat 
      tgtttcaaa cgcggggtt agggtgtct ttcaccttc 
      agaaaaatc aggaaaggg ggatgtaac tgcagttac 
      aaattacag tgcgattcg caaactgcg accaaaatt 
      attgagagc gatttgtta gcccataag ttggaaaat 
      caacacgtt catgatgtt tatgaggat attgccggg 
      cattttagc gaaactaga cacacaccg tggccaaat 
      gttctaaat tttgtgcaa aagctcgaa attggggct 
      gttttggtg gacgtgggg tgtggcaat gggaagtat 
      tttggacac aacagacaa attgtggag ttgggcacc 
      gaccgcagc ttcaaactc aacaacctt tgcaaacac 
      cgtggtttt gaagtcttc accggcaat tgcctcaac 
      ctccctcta aaaaactca tcagccgat gctgttatc 
      agcattgcc gtaatccac cacttatca acacccgaa 
      cgccggcta aaagccctc aaagaaatc gtccgaatc 
      cttcgaatt ggcggcgaa gctcttatt tacgtttgg 
      gcgaaacaa caaatcaaa aacgacgaa aaatcaacc 
      tacattaaa caagaccgg aaaaatcgg aaaaaggaa 
      gttatcgga agtgttacc aaggaggaa gttagtgtg 
      ggggagggt gtgacttta ccagtgcac acgaacagg 
      gcaaacttc acccacagt gatgtctta gtgccttgg 
      aaattgaaa ggggaggag caggagcgg acgtttttg 
      aggttttat cacgttttt gaggagggg gagcttgag 
      agtttgtgc caaagcatc gggaatgtt tccattgtg 
      aaaagttat tacgaccaa gggaattac tgtgtacag 
      tttgtaaaa aacaataaa taa
    mRNA sequence
    • >TC007671 RA
      ctaacctca gtttttgta ccaatggaa tctaacaat 
      tcccgcaaa aaactcatt aaaaaactg cgaaagcac 
      caacacact gttgggaaa gaaaccgga gtcgtctgt 
      accgacagc cccacgaaa aaccttgta atttgcaat 
      actggccaa attaacggc ttaactgag gaaataatc 
      gttgaacac ttcgaaaaa tacggtcac ctcgaccgc 
      gttttacta gttcctgga aaatcgtgc gcttttttg 
      agctataaa gacacctca agtgcttca gaagcattg 
      gaggctgtt agcggtaaa ttgaatata gcccaagac 
      ggcaaaccg atttttttg agttttgtt gaggctttg 
      cctgagata agggatgcg aaaatttgg gggcgcctc 
      ccgccaggt ttgaggata attacgaat tttgttagt 
      gaggaagaa gaggcaaga ttgttggct ttgtgccag 
      tttgaagat ggcggaagc atgaaacac agattagta 
      aaacactac ggttatgag tttcgttac gatattaac 
      aatgttgat aaagagaag ccgttaagt gagggaatc 
      ccccaagag tgcgatttt ttatggaga cgccttccg 
      tttgaattc cgccccaat cagttaaca atcaatcgg 
      tacaatccc ggacagggg attccctca cacgtggac 
      acccacagc gcgttcggg gaccccatc ctgtctcta 
      agcctcagt tctgacgtt gtgatggaa ttcaaaaaa 
      gacgaaacg atttgtgtt cttttaccc cgaaggtcg 
      ttacttgta atggcaggg gagagtagg tacgaatgg 
      acgcatggg atcgtgccc cgaactttt gatttttac 
      aatgatgaa gggggctgt cattgtttc aaacgcggg 
      gttagggtg tctttcacc ttcagaaaa atcaggaaa 
      gggggatgt aactgcagt tacaaatta cagtgcgat 
      tcgcaaact gcgaccaaa attattgag agcgatttg 
      ttagcccat aagttggaa aatcaacac gttcatgat 
      gtttatgag gatattgcc gggcatttt agcgaaact 
      agacacaca ccgtggcca aatgttcta aattttgtg 
      caaaagctc gaaattggg gctgttttg gtggacgtg 
      gggtgtggc aatgggaag tattttgga cacaacaga 
      caaattgtg gagttgggc accgaccgc agcttcaaa 
      ctcaacaac ctttgcaaa caccgtggt tttgaagtc 
      ttcaccggc aattgcctc aacctccct ctaaaaaac 
      tcatcagcc gatgctgtt atcagcatt gccgtaatc 
      caccactta tcaacaccc gaacgccgg ctaaaagcc 
      ctcaaagaa atcgtccga atccttcga attggcggc 
      gaagctctt atttacgtt tgggcgaaa caacaaatc 
      aaaaacgac gaaaaatca acctacatt aaacaagac 
      cggaaaaat cggaaaaag gaagttatc ggaagtgtt 
      accaaggag gaagttagt gtgggggag ggtgtgact 
      ttaccagtg cacacgaac agggcaaac ttcacccac 
      agtgatgtc ttagtgcct tggaaattg aaaggggag 
      gagcaggag cggacgttt ttgaggttt tatcacgtt 
      tttgaggag ggggagctt gagagtttg tgccaaagc 
      atcgggaat gtttccatt gtgaaaagt tattacgac 
      caagggaat tactgtgta cagtttgta aaaaacaat 
      aaataaact aaaagtaaa ataacacgt ttttaccaa 
      tcctataca agaattgga ccaaaaaat gacatttat 
      cgagctaaa aattcaatt atctcagtt ttttccgac 
      caaaaagtc aataaatta gagaaaatt at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY