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Gene information
General information
TC007664 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1188182..1190555
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007664
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04361

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partiallyorientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: partially or complete insideout
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva anterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head mostlynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007664 RA
      MNQDDKPTG KSQTSVLPP IQEATKSPK KKISGDLTA 
      ASLLHSITQ EKKLPQNIS HLNTALPEK KEPELPSQD 
      IVLKPELSS FDRISERHQ HSARSSIVS IESKKSQRL 
      KNDTDLSSE DIRTSESYF SETARTSQM FGRRYQKSD 
      QLVTNNKHP LDDLDPAGI VRAMKDLQR KVQHLKVEN 
      EIFYNFLQE NDSEAIKEM AELQEKRRI NADRVQAAV 
      KKRASRGSV VSTTNLGFG ASGPRGSIA SLGAKPRTS 
      VASITGRPR ASLASFSHF ESDLYSDKE APKIILAQK 
      LDMIMKCME EFEKHLETA RNQSKKAKR TLLSNLEEM 
      EIRNEEIQE SREHFAELR NNGVTLEKF VRFFEEWLK 
      KSRLTGDKI RLRHSSLKV QYHKAATLL ANKKLIGEH 
      VQEVDFDQL RVENRHLTE HIESKNCLL IEMKKMTAS 
      GNLKLTQLK KFLQEKMAK KEALLQSIK TFEDDIMRS 
      DEQLEKLYK ELDQVRKKY DQCKERSEN YLAPDVLEY 
      IRKKADVDN AKKELKVWE RRKMLKELA LSIAITEMK 
      RIFRCTHTH PSWFK
    CDS sequence
    • >TC007664 RA
      atgaaccaa gatgataaa cctactgga aaaagtcag 
      accagtgtt ttgccccca atacaagag gcaaccaaa 
      agccccaag aaaaaaatt tcaggggac ttgactgca 
      gcttcctta cttcattca ataacacaa gagaaaaaa 
      cttccccaa aacatatct cacttaaat accgcacta 
      ccagagaaa aaagaaccc gagcttccg tctcaagat 
      atcgtctta aaacctgaa ctaagttct tttgacaga 
      atttctgaa cgtcaccaa cattccgcc cgttcgtct 
      attgtgagc atagaaagt aaaaagtca caacgtttg 
      aaaaatgac accgatttg tcgtctgaa gatatacga 
      acttcagag tcatatttt tccgaaact gcaagaacc 
      tcccaaatg tttggtaga cggtaccaa aagtctgac 
      caacttgta actaataac aaacatcca cttgatgac 
      cttgaccct gctggtatt gtaagagca atgaaagac 
      cttcaacga aaagtgcaa cacttgaag gttgaaaac 
      gagattttc tacaatttt ttgcaagaa aatgactcc 
      gaagccatc aaagaaatg gcagaactg caggaaaaa 
      cggcgtatt aatgccgat agagtacaa gcagctgtt 
      aagaaaaga gcatcgaga ggtagcgtc gtctccact 
      acaaactta ggtttcgga gcttcaggc ccaagagga 
      tctattgcc tccttgggc gcaaaacca cgaacttcc 
      gtagcttcg atcacagga cggccacga gcatcactg 
      gcttcgttc tcacacttt gagtccgat ctttactct 
      gataaagaa gcaccgaaa ataatcctc gcccaaaaa 
      ctggacatg atcatgaaa tgtatggaa gagtttgaa 
      aaacacctc gaaacggct agaaatcag tcgaaaaaa 
      gcaaaacgt acgctgctc tcaaacctc gaagagatg 
      gaaatcagg aacgaggaa atccaagaa tcgcgggaa 
      catttcgca gaattgaga aacaacggc gtcacgctt 
      gaaaaattc gtccggttt ttcgaggaa tggttgaag 
      aaatcacgc ctaactggg gacaaaatc agactgaga 
      cacagttcc cttaaggtg caataccac aaagctgcg 
      actcttctc gctaataag aaactaata ggggaacac 
      gtgcaggag gttgatttt gatcaactc cgagtcgag 
      aatcggcat ttgactgag catattgag agcaaaaac 
      tgtcttctt atcgaaatg aagaaaatg acagccagt 
      ggtaattta aagttgact cagttgaaa aaattccta 
      caggagaaa atggccaag aaggaagcg ctgcttcaa 
      agtattaaa acgtttgaa gatgatatt atgcggtcg 
      gatgagcag ttagagaag ctttataaa gagctggat 
      caagtcagg aaaaagtac gaccagtgc aaagaacga 
      tctgaaaat tatttggcg cccgatgtt ttggagtat 
      attcgaaag aaggcagat gttgataat gcaaaaaaa 
      gagttgaaa gtttgggaa aggcgaaag atgctgaag 
      gaactagct ctgagtatt gcaataact gaaatgaag 
      aggattttc aggtgtaca catacacac ccgtcttgg 
      ttcaaatag
    mRNA sequence
    • >TC007664 RA
      ttgttgatg attataaaa gtataaaaa atatgtttt 
      tctcatttt tatgaaggt cgattgtgt cattagcca 
      ttaatttcc tcctcttgt gccataatt gcttggaag 
      tggcacagg tcatgtcga aattgcgca ttattagct 
      aatatgatg atattacat aacattttc gaagccggc 
      cggacgttc ctcgtttcc tgaccacta aacgatagc 
      ttcatcaat tcataaacg tggcacatt accggtctt 
      tacccaagt acgtaaaca taatattcc ctccttcct 
      gttaattat gcaattctt aaggagagc tgccaaacg 
      ggctcccgg ccaaagaga gcaattatg gaaggatta 
      ccttgatgt gtaattgca gagctgcat ccttggccg 
      gcgcacttt ccttatcag agcttaatg ctgatgatt 
      atgtgtctg gaccaagtt tttcccaga cattaaacc 
      taattagat cactcaact gtgcacaaa atcacatag 
      aaactaaac atttcaaac aaatgtcat ttaattgat 
      ttgtgtgtg tgtttgtgt tatttttgt aaaatgaac 
      caagatgat aaacctact ggaaaaagt cagaccagt 
      gttttgccc ccaatacaa gaggcaacc aaaagcccc 
      aagaaaaaa atttcaggg gacttgact gcagcttcc 
      ttacttcat tcaataaca caagagaaa aaacttccc 
      caaaacata tctcactta aataccgca ctaccagag 
      aaaaaagaa cccgagctt ccgtctcaa gatatcgtc 
      ttaaaacct gaactaagt tcttttgac agaatttct 
      gaacgtcac caacattcc gcccgttcg tctattgtg 
      agcatagaa agtaaaaag tcacaacgt ttgaaaaat 
      gacaccgat ttgtcgtct gaagatata cgaacttca 
      gagtcatat ttttccgaa actgcaaga acctcccaa 
      atgtttggt agacggtac caaaagtct gaccaactt 
      gtaactaat aacaaacat ccacttgat gaccttgac 
      cctgctggt attgtaaga gcaatgaaa gaccttcaa 
      cgaaaagtg caacacttg aaggttgaa aacgagatt 
      ttctacaat tttttgcaa gaaaatgac tccgaagcc 
      atcaaagaa atggcagaa ctgcaggaa aaacggcgt 
      attaatgcc gatagagta caagcagct gttaagaaa 
      agagcatcg agaggtagc gtcgtctcc actacaaac 
      ttaggtttc ggagcttca ggcccaaga ggatctatt 
      gcctccttg ggcgcaaaa ccacgaact tccgtagct 
      tcgatcaca ggacggcca cgagcatca ctggcttcg 
      ttctcacac tttgagtcc gatctttac tctgataaa 
      gaagcaccg aaaataatc ctcgcccaa aaactggac 
      atgatcatg aaatgtatg gaagagttt gaaaaacac 
      ctcgaaacg gctagaaat cagtcgaaa aaagcaaaa 
      cgtacgctg ctctcaaac ctcgaagag atggaaatc 
      aggaacgag gaaatccaa gaatcgcgg gaacatttc 
      gcagaattg agaaacaac ggcgtcacg cttgaaaaa 
      ttcgtccgg tttttcgag gaatggttg aagaaatca 
      cgcctaact ggggacaaa atcagactg agacacagt 
      tcccttaag gtgcaatac cacaaagct gcgactctt 
      ctcgctaat aagaaacta ataggggaa cacgtgcag 
      gaggttgat tttgatcaa ctccgagtc gagaatcgg 
      catttgact gagcatatt gagagcaaa aactgtctt 
      cttatcgaa atgaagaaa atgacagcc agtggtaat 
      ttaaagttg actcagttg aaaaaattc ctacaggag 
      aaaatggcc aagaaggaa gcgctgctt caaagtatt 
      aaaacgttt gaagatgat attatgcgg tcggatgag 
      cagttagag aagctttat aaagagctg gatcaagtc 
      aggaaaaag tacgaccag tgcaaagaa cgatctgaa 
      aattatttg gcgcccgat gttttggag tatattcga 
      aagaaggca gatgttgat aatgcaaaa aaagagttg 
      aaagtttgg gaaaggcga aagatgctg aaggaacta 
      gctctgagt attgcaata actgaaatg aagaggatt 
      ttcaggtgt acacataca cacccgtct tggttcaaa 
      taggctcta attgtgcgt tgtgttagt ttttttaat 
      ttgtgtttt aaataaatg ataccgtga aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY