| General information | |||
| TC007656 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1427840..1432549 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC007656 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04360 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdominal segment 1 present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC007656 RA
MSSPIMKSL GTLPDKYES LSLSLSPSD DPKCESVHL LDDQERLLI STEENFVKL LKIDPTHKI KVVSIFGNT GEGKSYTLN HTFFEGREI FQTSPYQNS CTIGVWAKY DPTLKMLCI DTEGFLGIT KKEHQRTRL LLKILAISD VIIYRTKSE RLPRDMYTF LGGASKAYK EHFSPALQS VLQKYEVEK TAAAWGPSV IIFHETRYT NTLTSIADV KMSPEDILR ENFAKLDES CDGFSSLKY IGVVDGVGK ASFLQLRVA VEKELESNQ VRSPRSPKY VFHTLKALN EKFQSHISD IAPQLYLSA FFTCQDTCL SCGVGCTLS MGHKDDDEP HLNLSNCKL QHQYQNFVY LCKKCYENG RKQVVEPSY QGKNEQFWS SIISSVWSG YVIECPTCG KIYQSREYW YGNKDPKDE AVVIDIVHV WPGHKSLLG SQNSAQKVL DGVTSISNV VASVGSQPT KMISDWVTD QIAPGYWRP NNEILECFK CKTAFESNA SKHHCRACG EGFCEGCSS KSQPVPERG WHEDVRVCD DCYKEETPT INHVADGTE LRSRRIGES VINSISVVK SILDKPKEL IKETARPAY WTPDNECRN CAVCERPFG ALLGLHHCR DCGKGVCDK CSTTRKPVP LRGWETPVR VCDKCSS
- >TC007656 RB
MLTQINTTL LGYSKIQKL LPPHVQNSK KMSSPIMKS LGTLPDKYE SLSLSLSPS DDPKCESVH LLDDQERLL ISTEENFVK LLKIDPTHK IKVVSIFGN TGEGKSYTL NHTFFEGRE IFQTSPYQN SCTIGVWAK YDPTLKMLC IDTEGFLGI TKKEHQRTR LLLKILAIS DVIIYRTKS ERLPRDMYT FLGGASKAY KEHFSPALQ SVLQKYEVE KTAAAWGPS VIIFHETRY TNTLTSIAD VKMSPEDIL RENFAKLDE SCDGFSSLK YIGVVDGVG KASFLQLRV AVEKELESN QVRSPRSPK YVFHTLKAL NEKFQSHIS DIAPQLYLS AFFTCQDTC LSCGVGCTL SMGHKDDDE PHLNLSNCK LQHQYQNFV YLCKKCYEN GRKQVVEPS YQGKNEQFW SSIISSVWS GYVIECPTC GKIYQSREY WYGNKDPKD EAVVIDIVH VWPGHKSLL GSQNSAQKV LDGVTSISN VVASVGSQP TKMISDWVT DQIAPGYWR PNNEILECF KCKTAFESN ASKHHCRAC GEGFCEGCS SKSQPVPER GWHEDVRVC DDCYKEETP TINHVADGT ELRSRRIGE SVINSISVV KSILDKPKE LIKETARPA YWTPDNECR NCAVCERPF GALLGLHHC RDCGKGVCD KCSTTRKPV PLRGWETPV RVCDKCSS
- >TC007656 RA
atgtcttcc cctataatg aagtcgctg ggcacttta cccgacaaa tacgagtct ttgagtctc tccctgagc ccctctgac gacccaaaa tgcgaaagc gtgcacctt ttagacgac caagagcgc ctcttgatc agcaccgaa gaaaacttc gtcaaacta ctaaaaatc gaccccact cacaaaatc aaagtcgtt tcgattttt ggtaacaca ggagagggc aagtcttac accctcaac cacacattc ttcgaaggc agggaaata ttccaaact tccccgtac cagaactct tgcacgatc ggcgtttgg gcaaaatac gaccccacc ttaaaaatg ctctgcatc gacactgaa ggcttcctg gggatcact aaaaaggag caccaacgc acccgtctc ctgttaaaa attctcgca atttccgac gttatcatc tatcgcacg aaatcagag cgtttgcca cgcgacatg tacacgttc ttggggggc gcctccaag gcgtataaa gagcacttc agtcctgcg ttacaaagc gttttgcaa aagtacgaa gtggaaaag actgcagcc gcatggggg cccagtgtt attattttc cacgagacg cggtatacg aatactttg accagtatc gcagatgtt aaaatgtcg ccagaggat attttacgg gagaatttc gccaaattg gatgagagt tgtgatggg tttagctca ttgaagtac attggggtg gtagatggg gtcgggaag gcgtcgttt ttgcaattg agggttgct gtggagaag gagctcgaa agtaatcaa gtgcggtcg ccgaggagc cccaaatat gtctttcat acgctgaag gcgttaaat gagaaattt caaagccac atatccgac atcgccccc cagctctac ctctcggcg tttttcacg tgccaagac acatgttta tcatgtggg gtgggttgt actttatca atgggtcac aaagacgac gatgaacca cacttgaat ttaagcaac tgcaagctc cagcatcaa taccaaaat ttcgtttat ctttgcaaa aaatgttac gaaaacggc cgaaagcaa gtagttgaa ccaagctat caaggaaaa aacgaacag ttctggagc agcattatc tcatcagtc tggtcgggt tatgtaatc gaatgccca acttgtggt aaaatctac caaagtcgg gaatactgg tacggtaat aaggacccg aaagacgag gctgtggta attgacatt gtccatgtc tggccaggg cataaatca ctgctgggg tcccaaaat tcggcccag aaagtccta gatggggtc actagcatc agtaatgta gtagccagc gttggatct cagcccact aagatgata agtgattgg gtgaccgat caaatcgcc cccggctac tggcgaccc aacaacgaa attttggag tgcttcaag tgcaagaca gcgtttgag tcgaatgct tcaaagcac cactgtcgc gcctgtggg gagggtttc tgcgagggt tgttcgtcc aaaagccag ccggtgcct gaaaggggt tggcatgaa gacgtgcga gtttgtgac gattgttac aaggaggag acgccaact atcaaccac gtggccgat ggcacggaa cttcgcagt cggagaata ggagaaagc gttataaac tcgatatca gtcgttaag tcgattctg gacaagccg aaagaactg ataaaggaa actgcaagg ccggcgtac tggacgccc gataacgaa tgcagaaac tgtgcagta tgtgagaga ccgtttggg gcgcttttg gggctgcac cactgccgg gattgtggt aagggggtg tgtgataaa tgctcgacg acgcggaaa ccggtgcca ctgcgaggg tgggaaaca ccagtccga gtttgtgat aaatgttca agttaa
- >TC007656 RB
atgttgaca caaataaac acaacgctg cttgggtat agcaaaatc caaaaacta ctaccgccc cacgtccaa aattccaaa aaaatgtct tcccctata atgaagtcg ctgggcact ttacccgac aaatacgag tctttgagt ctctccctg agcccctct gacgaccca aaatgcgaa agcgtgcac cttttagac gaccaagag cgcctcttg atcagcacc gaagaaaac ttcgtcaaa ctactaaaa atcgacccc actcacaaa atcaaagtc gtttcgatt tttggtaac acaggagag ggcaagtct tacaccctc aaccacaca ttcttcgaa ggcagggaa atattccaa acttccccg taccagaac tcttgcacg atcggcgtt tgggcaaaa tacgacccc accttaaaa atgctctgc atcgacact gaaggcttc ctggggatc actaaaaag gagcaccaa cgcacccgt ctcctgtta aaaattctc gcaatttcc gacgttatc atctatcgc acgaaatca gagcgtttg ccacgcgac atgtacacg ttcttgggg ggcgcctcc aaggcgtat aaagagcac ttcagtcct gcgttacaa agcgttttg caaaagtac gaagtggaa aagactgca gccgcatgg gggcccagt gttattatt ttccacgag acgcggtat acgaatact ttgaccagt atcgcagat gttaaaatg tcgccagag gatatttta cgggagaat ttcgccaaa ttggatgag agttgtgat gggtttagc tcattgaag tacattggg gtggtagat ggggtcggg aaggcgtcg tttttgcaa ttgagggtt gctgtggag aaggagctc gaaagtaat caagtgcgg tcgccgagg agccccaaa tatgtcttt catacgctg aaggcgtta aatgagaaa tttcaaagc cacatatcc gacatcgcc ccccagctc tacctctcg gcgtttttc acgtgccaa gacacatgt ttatcatgt ggggtgggt tgtacttta tcaatgggt cacaaagac gacgatgaa ccacacttg aatttaagc aactgcaag ctccagcat caataccaa aatttcgtt tatctttgc aaaaaatgt tacgaaaac ggccgaaag caagtagtt gaaccaagc tatcaagga aaaaacgaa cagttctgg agcagcatt atctcatca gtctggtcg ggttatgta atcgaatgc ccaacttgt ggtaaaatc taccaaagt cgggaatac tggtacggt aataaggac ccgaaagac gaggctgtg gtaattgac attgtccat gtctggcca gggcataaa tcactgctg gggtcccaa aattcggcc cagaaagtc ctagatggg gtcactagc atcagtaat gtagtagcc agcgttgga tctcagccc actaagatg ataagtgat tgggtgacc gatcaaatc gcccccggc tactggcga cccaacaac gaaattttg gagtgcttc aagtgcaag acagcgttt gagtcgaat gcttcaaag caccactgt cgcgcctgt ggggagggt ttctgcgag ggttgttcg tccaaaagc cagccggtg cctgaaagg ggttggcat gaagacgtg cgagtttgt gacgattgt tacaaggag gagacgcca actatcaac cacgtggcc gatggcacg gaacttcgc agtcggaga ataggagaa agcgttata aactcgata tcagtcgtt aagtcgatt ctggacaag ccgaaagaa ctgataaag gaaactgca aggccggcg tactggacg cccgataac gaatgcaga aactgtgca gtatgtgag agaccgttt ggggcgctt ttggggctg caccactgc cgggattgt ggtaagggg gtgtgtgat aaatgctcg acgacgcgg aaaccggtg ccactgcga gggtgggaa acaccagtc cgagtttgt gataaatgt tcaagttaa
- >TC007656 RA
agcacgtag acaaacaat cacggtcaa aaacctcgc caacaatca gaaattcag aaatagcca caattctgg aatgaattt tcagcgaca aaaacaaaa ataaaattc ttgcggtgt gtccttgag tgcaacagg aacagtcag tgtagcact acacccaat tggaatcaa ctgtcaaaa acatcaatt caagtgtca agtcatgtg taagtagtt tattaacaa aaaacgtgt atcatgtct aggaaaacg caaagctgt gacacaaat aaacacaac gctgcttgg gtatagcaa aatccaaaa actactacc gccccacgt ccaaaattc caaaaaaat gtcttcccc tataatgaa gtcgctggg cactttacc cgacaaata cgagtcttt gagtctctc cctgagccc ctctgacga cccaaaatg cgaaagcgt gcacctttt agacgacca agagcgcct cttgatcag caccgaaga aaacttcgt caaactact aaaaatcga ccccactca caaaatcaa agtcgtttc gatttttgg taacacagg agagggcaa gtcttacac cctcaacca cacattctt cgaaggcag ggaaatatt ccaaacttc cccgtacca gaactcttg cacgatcgg cgtttgggc aaaatacga ccccacctt aaaaatgct ctgcatcga cactgaagg cttcctggg gatcactaa aaaggagca ccaacgcac ccgtctcct gttaaaaat tctcgcaat ttccgacgt tatcatcta tcgcacgaa atcagagcg tttgccacg cgacatgta cacgttctt ggggggcgc ctccaaggc gtataaaga gcacttcag tcctgcgtt acaaagcgt tttgcaaaa gtacgaagt ggaaaagac tgcagccgc atgggggcc cagtgttat tattttcca cgagacgcg gtatacgaa tactttgac cagtatcgc agatgttaa aatgtcgcc agaggatat tttacggga gaatttcgc caaattgga tgagagttg tgatgggtt tagctcatt gaagtacat tggggtggt agatggggt cgggaaggc gtcgttttt gcaattgag ggttgctgt ggagaagga gctcgaaag taatcaagt gcggtcgcc gaggagccc caaatatgt ctttcatac gctgaaggc gttaaatga gaaatttca aagccacat atccgacat cgcccccca gctctacct ctcggcgtt tttcacgtg ccaagacac atgtttatc atgtggggt gggttgtac tttatcaat gggtcacaa agacgacga tgaaccaca cttgaattt aagcaactg caagctcca gcatcaata ccaaaattt cgtttatct ttgcaaaaa atgttacga aaacggccg aaagcaagt agttgaacc aagctatca aggaaaaaa cgaacagtt ctggagcag cattatctc atcagtctg gtcgggtta tgtaatcga atgcccaac ttgtggtaa aatctacca aagtcggga atactggta cggtaataa ggacccgaa agacgaggc tgtggtaat tgacattgt ccatgtctg gccagggca taaatcact gctggggtc ccaaaattc ggcccagaa agtcctaga tggggtcac tagcatcag taatgtagt agccagcgt tggatctca gcccactaa gatgataag tgattgggt gaccgatca aatcgcccc cggctactg gcgacccaa caacgaaat tttggagtg cttcaagtg caagacagc gtttgagtc gaatgcttc aaagcacca ctgtcgcgc ctgtgggga gggtttctg cgagggttg ttcgtccaa aagccagcc ggtgcctga aaggggttg gcatgaaga cgtgcgagt ttgtgacga ttgttacaa ggaggagac gccaactat caaccacgt ggccgatgg cacggaact tcgcagtcg gagaatagg agaaagcgt tataaactc gatatcagt cgttaagtc gattctgga caagccgaa agaactgat aaaggaaac tgcaaggcc ggcgtactg gacgcccga taacgaatg cagaaactg tgcagtatg tgagagacc gtttggggc gcttttggg gctgcacca ctgccggga ttgtggtaa gggggtgtg tgataaatg ctcgacgac gcggaaacc ggtgccact gcgagggtg ggaaacacc agtccgagt ttgtgataa atgttcaag ttaagatgc tcaaaattt gttacaagt ctgagtaag gacacagta gctttcgat aaagcattt acgtaggtt tgtactttt aataaatcc gtgtgaacg tccttgtgt ggtcttttt tattttata gggttatgg aaatatacg ccggttaga gattatgct tgttaataa cttatagtt tattttatt gtgacggag tgagtgatg taatattta cctcgctca gtttttcac taaaacggt attttttat taggtttcg agtcacaga actgtaccc cttagagta tttcaataa cacaaatgt gtgctttta taacaagtc caattgtca gtatagaat gtttttttt ggaataatt ttttttctt gtacagtgg cagattttg tatcaaatg tacatacat ttaggcgaa ttttttatt agaaaatta aggtgttta aattaagtt tcaagcttt acattccaa attttaatg tgagcaggt tactactaa aaatttgtg catttatga tcaatttat ttctttatt tgttatttt catcaaagc atcgacaca aaaactatt tttagtgat gacaatttc attaaattc atattgtga tacaatgta aatacttag ttgtatgta cataataaa attgttcat ttaac
- >TC007656 RB
agcacgtag acaaacaat cacggtcaa aaacctcgc caacaatca gaaattcag aaatagcca caattctgg aatgaattt tcagcgaca aaaacaaaa ataaaattc ttgcggtgt gtccttgag tgcaacagg aacagtcag tgtagcact acacccaat tggaatcaa ctgtcaaaa acatcaatt caagtgtca agtcatgtt gacacaaat aaacacaac gctgcttgg gtatagcaa aatccaaaa actactacc gccccacgt ccaaaattc caaaaaaat gtcttcccc tataatgaa gtcgctggg cactttacc cgacaaata cgagtcttt gagtctctc cctgagccc ctctgacga cccaaaatg cgaaagcgt gcacctttt agacgacca agagcgcct cttgatcag caccgaaga aaacttcgt caaactact aaaaatcga ccccactca caaaatcaa agtcgtttc gatttttgg taacacagg agagggcaa gtcttacac cctcaacca cacattctt cgaaggcag ggaaatatt ccaaacttc cccgtacca gaactcttg cacgatcgg cgtttgggc aaaatacga ccccacctt aaaaatgct ctgcatcga cactgaagg cttcctggg gatcactaa aaaggagca ccaacgcac ccgtctcct gttaaaaat tctcgcaat ttccgacgt tatcatcta tcgcacgaa atcagagcg tttgccacg cgacatgta cacgttctt ggggggcgc ctccaaggc gtataaaga gcacttcag tcctgcgtt acaaagcgt tttgcaaaa gtacgaagt ggaaaagac tgcagccgc atgggggcc cagtgttat tattttcca cgagacgcg gtatacgaa tactttgac cagtatcgc agatgttaa aatgtcgcc agaggatat tttacggga gaatttcgc caaattgga tgagagttg tgatgggtt tagctcatt gaagtacat tggggtggt agatggggt cgggaaggc gtcgttttt gcaattgag ggttgctgt ggagaagga gctcgaaag taatcaagt gcggtcgcc gaggagccc caaatatgt ctttcatac gctgaaggc gttaaatga gaaatttca aagccacat atccgacat cgcccccca gctctacct ctcggcgtt tttcacgtg ccaagacac atgtttatc atgtggggt gggttgtac tttatcaat gggtcacaa agacgacga tgaaccaca cttgaattt aagcaactg caagctcca gcatcaata ccaaaattt cgtttatct ttgcaaaaa atgttacga aaacggccg aaagcaagt agttgaacc aagctatca aggaaaaaa cgaacagtt ctggagcag cattatctc atcagtctg gtcgggtta tgtaatcga atgcccaac ttgtggtaa aatctacca aagtcggga atactggta cggtaataa ggacccgaa agacgaggc tgtggtaat tgacattgt ccatgtctg gccagggca taaatcact gctggggtc ccaaaattc ggcccagaa agtcctaga tggggtcac tagcatcag taatgtagt agccagcgt tggatctca gcccactaa gatgataag tgattgggt gaccgatca aatcgcccc cggctactg gcgacccaa caacgaaat tttggagtg cttcaagtg caagacagc gtttgagtc gaatgcttc aaagcacca ctgtcgcgc ctgtgggga gggtttctg cgagggttg ttcgtccaa aagccagcc ggtgcctga aaggggttg gcatgaaga cgtgcgagt ttgtgacga ttgttacaa ggaggagac gccaactat caaccacgt ggccgatgg cacggaact tcgcagtcg gagaatagg agaaagcgt tataaactc gatatcagt cgttaagtc gattctgga caagccgaa agaactgat aaaggaaac tgcaaggcc ggcgtactg gacgcccga taacgaatg cagaaactg tgcagtatg tgagagacc gtttggggc gcttttggg gctgcacca ctgccggga ttgtggtaa gggggtgtg tgataaatg ctcgacgac gcggaaacc ggtgccact gcgagggtg ggaaacacc agtccgagt ttgtgataa atgttcaag ttaagatgc tcaaaattt gttacaagt ctgagtaag gacacagta gctttcgat aaagcattt acgtaggtt tgtactttt aataaatcc gtgtgaacg tccttgtgt ggtcttttt tattttata gggttatgg aaatatacg ccggttaga gattatgct tgttaataa cttatagtt tattttatt gtgacggag tgagtgatg taatattta cctcgctca gtttttcac taaaacggt attttttat taggtttcg agtcacaga actgtaccc cttagagta tttcaataa cacaaatgt gtgctttta taacaagtc caattgtca gtatagaat gtttttttt ggaataatt ttttttctt gtacagtgg cagattttg tatcaaatg tacatacat ttaggcgaa ttttttatt agaaaatta aggtgttta aattaagtt tcaagcttt acattccaa attttaatg tgagcaggt tactactaa aaatttgtg catttatga tcaatttat ttctttatt tgttatttt catcaaagc atcgacaca aaaactatt tttagtgat gacaatttc attaaattc atattgtga tacaatgta aatacttag ttgtatgta cataataaa attgttcat ttaac
