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Gene information
General information
TC007656 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1427840..1432549
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007656
    iBeetle screen iB_04360

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdominal segment 1 present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007656 RA
      MSSPIMKSL GTLPDKYES LSLSLSPSD DPKCESVHL 
      LDDQERLLI STEENFVKL LKIDPTHKI KVVSIFGNT 
      GEGKSYTLN HTFFEGREI FQTSPYQNS CTIGVWAKY 
      DPTLKMLCI DTEGFLGIT KKEHQRTRL LLKILAISD 
      VIIYRTKSE RLPRDMYTF LGGASKAYK EHFSPALQS 
      VLQKYEVEK TAAAWGPSV IIFHETRYT NTLTSIADV 
      KMSPEDILR ENFAKLDES CDGFSSLKY IGVVDGVGK 
      ASFLQLRVA VEKELESNQ VRSPRSPKY VFHTLKALN 
      EKFQSHISD IAPQLYLSA FFTCQDTCL SCGVGCTLS 
      MGHKDDDEP HLNLSNCKL QHQYQNFVY LCKKCYENG 
      RKQVVEPSY QGKNEQFWS SIISSVWSG YVIECPTCG 
      KIYQSREYW YGNKDPKDE AVVIDIVHV WPGHKSLLG 
      SQNSAQKVL DGVTSISNV VASVGSQPT KMISDWVTD 
      QIAPGYWRP NNEILECFK CKTAFESNA SKHHCRACG 
      EGFCEGCSS KSQPVPERG WHEDVRVCD DCYKEETPT 
      INHVADGTE LRSRRIGES VINSISVVK SILDKPKEL 
      IKETARPAY WTPDNECRN CAVCERPFG ALLGLHHCR 
      DCGKGVCDK CSTTRKPVP LRGWETPVR VCDKCSS
    • >TC007656 RB
      MLTQINTTL LGYSKIQKL LPPHVQNSK KMSSPIMKS 
      LGTLPDKYE SLSLSLSPS DDPKCESVH LLDDQERLL 
      ISTEENFVK LLKIDPTHK IKVVSIFGN TGEGKSYTL 
      NHTFFEGRE IFQTSPYQN SCTIGVWAK YDPTLKMLC 
      IDTEGFLGI TKKEHQRTR LLLKILAIS DVIIYRTKS 
      ERLPRDMYT FLGGASKAY KEHFSPALQ SVLQKYEVE 
      KTAAAWGPS VIIFHETRY TNTLTSIAD VKMSPEDIL 
      RENFAKLDE SCDGFSSLK YIGVVDGVG KASFLQLRV 
      AVEKELESN QVRSPRSPK YVFHTLKAL NEKFQSHIS 
      DIAPQLYLS AFFTCQDTC LSCGVGCTL SMGHKDDDE 
      PHLNLSNCK LQHQYQNFV YLCKKCYEN GRKQVVEPS 
      YQGKNEQFW SSIISSVWS GYVIECPTC GKIYQSREY 
      WYGNKDPKD EAVVIDIVH VWPGHKSLL GSQNSAQKV 
      LDGVTSISN VVASVGSQP TKMISDWVT DQIAPGYWR 
      PNNEILECF KCKTAFESN ASKHHCRAC GEGFCEGCS 
      SKSQPVPER GWHEDVRVC DDCYKEETP TINHVADGT 
      ELRSRRIGE SVINSISVV KSILDKPKE LIKETARPA 
      YWTPDNECR NCAVCERPF GALLGLHHC RDCGKGVCD 
      KCSTTRKPV PLRGWETPV RVCDKCSS
    CDS sequence
    • >TC007656 RA
      atgtcttcc cctataatg aagtcgctg ggcacttta 
      cccgacaaa tacgagtct ttgagtctc tccctgagc 
      ccctctgac gacccaaaa tgcgaaagc gtgcacctt 
      ttagacgac caagagcgc ctcttgatc agcaccgaa 
      gaaaacttc gtcaaacta ctaaaaatc gaccccact 
      cacaaaatc aaagtcgtt tcgattttt ggtaacaca 
      ggagagggc aagtcttac accctcaac cacacattc 
      ttcgaaggc agggaaata ttccaaact tccccgtac 
      cagaactct tgcacgatc ggcgtttgg gcaaaatac 
      gaccccacc ttaaaaatg ctctgcatc gacactgaa 
      ggcttcctg gggatcact aaaaaggag caccaacgc 
      acccgtctc ctgttaaaa attctcgca atttccgac 
      gttatcatc tatcgcacg aaatcagag cgtttgcca 
      cgcgacatg tacacgttc ttggggggc gcctccaag 
      gcgtataaa gagcacttc agtcctgcg ttacaaagc 
      gttttgcaa aagtacgaa gtggaaaag actgcagcc 
      gcatggggg cccagtgtt attattttc cacgagacg 
      cggtatacg aatactttg accagtatc gcagatgtt 
      aaaatgtcg ccagaggat attttacgg gagaatttc 
      gccaaattg gatgagagt tgtgatggg tttagctca 
      ttgaagtac attggggtg gtagatggg gtcgggaag 
      gcgtcgttt ttgcaattg agggttgct gtggagaag 
      gagctcgaa agtaatcaa gtgcggtcg ccgaggagc 
      cccaaatat gtctttcat acgctgaag gcgttaaat 
      gagaaattt caaagccac atatccgac atcgccccc 
      cagctctac ctctcggcg tttttcacg tgccaagac 
      acatgttta tcatgtggg gtgggttgt actttatca 
      atgggtcac aaagacgac gatgaacca cacttgaat 
      ttaagcaac tgcaagctc cagcatcaa taccaaaat 
      ttcgtttat ctttgcaaa aaatgttac gaaaacggc 
      cgaaagcaa gtagttgaa ccaagctat caaggaaaa 
      aacgaacag ttctggagc agcattatc tcatcagtc 
      tggtcgggt tatgtaatc gaatgccca acttgtggt 
      aaaatctac caaagtcgg gaatactgg tacggtaat 
      aaggacccg aaagacgag gctgtggta attgacatt 
      gtccatgtc tggccaggg cataaatca ctgctgggg 
      tcccaaaat tcggcccag aaagtccta gatggggtc 
      actagcatc agtaatgta gtagccagc gttggatct 
      cagcccact aagatgata agtgattgg gtgaccgat 
      caaatcgcc cccggctac tggcgaccc aacaacgaa 
      attttggag tgcttcaag tgcaagaca gcgtttgag 
      tcgaatgct tcaaagcac cactgtcgc gcctgtggg 
      gagggtttc tgcgagggt tgttcgtcc aaaagccag 
      ccggtgcct gaaaggggt tggcatgaa gacgtgcga 
      gtttgtgac gattgttac aaggaggag acgccaact 
      atcaaccac gtggccgat ggcacggaa cttcgcagt 
      cggagaata ggagaaagc gttataaac tcgatatca 
      gtcgttaag tcgattctg gacaagccg aaagaactg 
      ataaaggaa actgcaagg ccggcgtac tggacgccc 
      gataacgaa tgcagaaac tgtgcagta tgtgagaga 
      ccgtttggg gcgcttttg gggctgcac cactgccgg 
      gattgtggt aagggggtg tgtgataaa tgctcgacg 
      acgcggaaa ccggtgcca ctgcgaggg tgggaaaca 
      ccagtccga gtttgtgat aaatgttca agttaa
    • >TC007656 RB
      atgttgaca caaataaac acaacgctg cttgggtat 
      agcaaaatc caaaaacta ctaccgccc cacgtccaa 
      aattccaaa aaaatgtct tcccctata atgaagtcg 
      ctgggcact ttacccgac aaatacgag tctttgagt 
      ctctccctg agcccctct gacgaccca aaatgcgaa 
      agcgtgcac cttttagac gaccaagag cgcctcttg 
      atcagcacc gaagaaaac ttcgtcaaa ctactaaaa 
      atcgacccc actcacaaa atcaaagtc gtttcgatt 
      tttggtaac acaggagag ggcaagtct tacaccctc 
      aaccacaca ttcttcgaa ggcagggaa atattccaa 
      acttccccg taccagaac tcttgcacg atcggcgtt 
      tgggcaaaa tacgacccc accttaaaa atgctctgc 
      atcgacact gaaggcttc ctggggatc actaaaaag 
      gagcaccaa cgcacccgt ctcctgtta aaaattctc 
      gcaatttcc gacgttatc atctatcgc acgaaatca 
      gagcgtttg ccacgcgac atgtacacg ttcttgggg 
      ggcgcctcc aaggcgtat aaagagcac ttcagtcct 
      gcgttacaa agcgttttg caaaagtac gaagtggaa 
      aagactgca gccgcatgg gggcccagt gttattatt 
      ttccacgag acgcggtat acgaatact ttgaccagt 
      atcgcagat gttaaaatg tcgccagag gatatttta 
      cgggagaat ttcgccaaa ttggatgag agttgtgat 
      gggtttagc tcattgaag tacattggg gtggtagat 
      ggggtcggg aaggcgtcg tttttgcaa ttgagggtt 
      gctgtggag aaggagctc gaaagtaat caagtgcgg 
      tcgccgagg agccccaaa tatgtcttt catacgctg 
      aaggcgtta aatgagaaa tttcaaagc cacatatcc 
      gacatcgcc ccccagctc tacctctcg gcgtttttc 
      acgtgccaa gacacatgt ttatcatgt ggggtgggt 
      tgtacttta tcaatgggt cacaaagac gacgatgaa 
      ccacacttg aatttaagc aactgcaag ctccagcat 
      caataccaa aatttcgtt tatctttgc aaaaaatgt 
      tacgaaaac ggccgaaag caagtagtt gaaccaagc 
      tatcaagga aaaaacgaa cagttctgg agcagcatt 
      atctcatca gtctggtcg ggttatgta atcgaatgc 
      ccaacttgt ggtaaaatc taccaaagt cgggaatac 
      tggtacggt aataaggac ccgaaagac gaggctgtg 
      gtaattgac attgtccat gtctggcca gggcataaa 
      tcactgctg gggtcccaa aattcggcc cagaaagtc 
      ctagatggg gtcactagc atcagtaat gtagtagcc 
      agcgttgga tctcagccc actaagatg ataagtgat 
      tgggtgacc gatcaaatc gcccccggc tactggcga 
      cccaacaac gaaattttg gagtgcttc aagtgcaag 
      acagcgttt gagtcgaat gcttcaaag caccactgt 
      cgcgcctgt ggggagggt ttctgcgag ggttgttcg 
      tccaaaagc cagccggtg cctgaaagg ggttggcat 
      gaagacgtg cgagtttgt gacgattgt tacaaggag 
      gagacgcca actatcaac cacgtggcc gatggcacg 
      gaacttcgc agtcggaga ataggagaa agcgttata 
      aactcgata tcagtcgtt aagtcgatt ctggacaag 
      ccgaaagaa ctgataaag gaaactgca aggccggcg 
      tactggacg cccgataac gaatgcaga aactgtgca 
      gtatgtgag agaccgttt ggggcgctt ttggggctg 
      caccactgc cgggattgt ggtaagggg gtgtgtgat 
      aaatgctcg acgacgcgg aaaccggtg ccactgcga 
      gggtgggaa acaccagtc cgagtttgt gataaatgt 
      tcaagttaa
    mRNA sequence
    • >TC007656 RA
      agcacgtag acaaacaat cacggtcaa aaacctcgc 
      caacaatca gaaattcag aaatagcca caattctgg 
      aatgaattt tcagcgaca aaaacaaaa ataaaattc 
      ttgcggtgt gtccttgag tgcaacagg aacagtcag 
      tgtagcact acacccaat tggaatcaa ctgtcaaaa 
      acatcaatt caagtgtca agtcatgtg taagtagtt 
      tattaacaa aaaacgtgt atcatgtct aggaaaacg 
      caaagctgt gacacaaat aaacacaac gctgcttgg 
      gtatagcaa aatccaaaa actactacc gccccacgt 
      ccaaaattc caaaaaaat gtcttcccc tataatgaa 
      gtcgctggg cactttacc cgacaaata cgagtcttt 
      gagtctctc cctgagccc ctctgacga cccaaaatg 
      cgaaagcgt gcacctttt agacgacca agagcgcct 
      cttgatcag caccgaaga aaacttcgt caaactact 
      aaaaatcga ccccactca caaaatcaa agtcgtttc 
      gatttttgg taacacagg agagggcaa gtcttacac 
      cctcaacca cacattctt cgaaggcag ggaaatatt 
      ccaaacttc cccgtacca gaactcttg cacgatcgg 
      cgtttgggc aaaatacga ccccacctt aaaaatgct 
      ctgcatcga cactgaagg cttcctggg gatcactaa 
      aaaggagca ccaacgcac ccgtctcct gttaaaaat 
      tctcgcaat ttccgacgt tatcatcta tcgcacgaa 
      atcagagcg tttgccacg cgacatgta cacgttctt 
      ggggggcgc ctccaaggc gtataaaga gcacttcag 
      tcctgcgtt acaaagcgt tttgcaaaa gtacgaagt 
      ggaaaagac tgcagccgc atgggggcc cagtgttat 
      tattttcca cgagacgcg gtatacgaa tactttgac 
      cagtatcgc agatgttaa aatgtcgcc agaggatat 
      tttacggga gaatttcgc caaattgga tgagagttg 
      tgatgggtt tagctcatt gaagtacat tggggtggt 
      agatggggt cgggaaggc gtcgttttt gcaattgag 
      ggttgctgt ggagaagga gctcgaaag taatcaagt 
      gcggtcgcc gaggagccc caaatatgt ctttcatac 
      gctgaaggc gttaaatga gaaatttca aagccacat 
      atccgacat cgcccccca gctctacct ctcggcgtt 
      tttcacgtg ccaagacac atgtttatc atgtggggt 
      gggttgtac tttatcaat gggtcacaa agacgacga 
      tgaaccaca cttgaattt aagcaactg caagctcca 
      gcatcaata ccaaaattt cgtttatct ttgcaaaaa 
      atgttacga aaacggccg aaagcaagt agttgaacc 
      aagctatca aggaaaaaa cgaacagtt ctggagcag 
      cattatctc atcagtctg gtcgggtta tgtaatcga 
      atgcccaac ttgtggtaa aatctacca aagtcggga 
      atactggta cggtaataa ggacccgaa agacgaggc 
      tgtggtaat tgacattgt ccatgtctg gccagggca 
      taaatcact gctggggtc ccaaaattc ggcccagaa 
      agtcctaga tggggtcac tagcatcag taatgtagt 
      agccagcgt tggatctca gcccactaa gatgataag 
      tgattgggt gaccgatca aatcgcccc cggctactg 
      gcgacccaa caacgaaat tttggagtg cttcaagtg 
      caagacagc gtttgagtc gaatgcttc aaagcacca 
      ctgtcgcgc ctgtgggga gggtttctg cgagggttg 
      ttcgtccaa aagccagcc ggtgcctga aaggggttg 
      gcatgaaga cgtgcgagt ttgtgacga ttgttacaa 
      ggaggagac gccaactat caaccacgt ggccgatgg 
      cacggaact tcgcagtcg gagaatagg agaaagcgt 
      tataaactc gatatcagt cgttaagtc gattctgga 
      caagccgaa agaactgat aaaggaaac tgcaaggcc 
      ggcgtactg gacgcccga taacgaatg cagaaactg 
      tgcagtatg tgagagacc gtttggggc gcttttggg 
      gctgcacca ctgccggga ttgtggtaa gggggtgtg 
      tgataaatg ctcgacgac gcggaaacc ggtgccact 
      gcgagggtg ggaaacacc agtccgagt ttgtgataa 
      atgttcaag ttaagatgc tcaaaattt gttacaagt 
      ctgagtaag gacacagta gctttcgat aaagcattt 
      acgtaggtt tgtactttt aataaatcc gtgtgaacg 
      tccttgtgt ggtcttttt tattttata gggttatgg 
      aaatatacg ccggttaga gattatgct tgttaataa 
      cttatagtt tattttatt gtgacggag tgagtgatg 
      taatattta cctcgctca gtttttcac taaaacggt 
      attttttat taggtttcg agtcacaga actgtaccc 
      cttagagta tttcaataa cacaaatgt gtgctttta 
      taacaagtc caattgtca gtatagaat gtttttttt 
      ggaataatt ttttttctt gtacagtgg cagattttg 
      tatcaaatg tacatacat ttaggcgaa ttttttatt 
      agaaaatta aggtgttta aattaagtt tcaagcttt 
      acattccaa attttaatg tgagcaggt tactactaa 
      aaatttgtg catttatga tcaatttat ttctttatt 
      tgttatttt catcaaagc atcgacaca aaaactatt 
      tttagtgat gacaatttc attaaattc atattgtga 
      tacaatgta aatacttag ttgtatgta cataataaa 
      attgttcat ttaac
    • >TC007656 RB
      agcacgtag acaaacaat cacggtcaa aaacctcgc 
      caacaatca gaaattcag aaatagcca caattctgg 
      aatgaattt tcagcgaca aaaacaaaa ataaaattc 
      ttgcggtgt gtccttgag tgcaacagg aacagtcag 
      tgtagcact acacccaat tggaatcaa ctgtcaaaa 
      acatcaatt caagtgtca agtcatgtt gacacaaat 
      aaacacaac gctgcttgg gtatagcaa aatccaaaa 
      actactacc gccccacgt ccaaaattc caaaaaaat 
      gtcttcccc tataatgaa gtcgctggg cactttacc 
      cgacaaata cgagtcttt gagtctctc cctgagccc 
      ctctgacga cccaaaatg cgaaagcgt gcacctttt 
      agacgacca agagcgcct cttgatcag caccgaaga 
      aaacttcgt caaactact aaaaatcga ccccactca 
      caaaatcaa agtcgtttc gatttttgg taacacagg 
      agagggcaa gtcttacac cctcaacca cacattctt 
      cgaaggcag ggaaatatt ccaaacttc cccgtacca 
      gaactcttg cacgatcgg cgtttgggc aaaatacga 
      ccccacctt aaaaatgct ctgcatcga cactgaagg 
      cttcctggg gatcactaa aaaggagca ccaacgcac 
      ccgtctcct gttaaaaat tctcgcaat ttccgacgt 
      tatcatcta tcgcacgaa atcagagcg tttgccacg 
      cgacatgta cacgttctt ggggggcgc ctccaaggc 
      gtataaaga gcacttcag tcctgcgtt acaaagcgt 
      tttgcaaaa gtacgaagt ggaaaagac tgcagccgc 
      atgggggcc cagtgttat tattttcca cgagacgcg 
      gtatacgaa tactttgac cagtatcgc agatgttaa 
      aatgtcgcc agaggatat tttacggga gaatttcgc 
      caaattgga tgagagttg tgatgggtt tagctcatt 
      gaagtacat tggggtggt agatggggt cgggaaggc 
      gtcgttttt gcaattgag ggttgctgt ggagaagga 
      gctcgaaag taatcaagt gcggtcgcc gaggagccc 
      caaatatgt ctttcatac gctgaaggc gttaaatga 
      gaaatttca aagccacat atccgacat cgcccccca 
      gctctacct ctcggcgtt tttcacgtg ccaagacac 
      atgtttatc atgtggggt gggttgtac tttatcaat 
      gggtcacaa agacgacga tgaaccaca cttgaattt 
      aagcaactg caagctcca gcatcaata ccaaaattt 
      cgtttatct ttgcaaaaa atgttacga aaacggccg 
      aaagcaagt agttgaacc aagctatca aggaaaaaa 
      cgaacagtt ctggagcag cattatctc atcagtctg 
      gtcgggtta tgtaatcga atgcccaac ttgtggtaa 
      aatctacca aagtcggga atactggta cggtaataa 
      ggacccgaa agacgaggc tgtggtaat tgacattgt 
      ccatgtctg gccagggca taaatcact gctggggtc 
      ccaaaattc ggcccagaa agtcctaga tggggtcac 
      tagcatcag taatgtagt agccagcgt tggatctca 
      gcccactaa gatgataag tgattgggt gaccgatca 
      aatcgcccc cggctactg gcgacccaa caacgaaat 
      tttggagtg cttcaagtg caagacagc gtttgagtc 
      gaatgcttc aaagcacca ctgtcgcgc ctgtgggga 
      gggtttctg cgagggttg ttcgtccaa aagccagcc 
      ggtgcctga aaggggttg gcatgaaga cgtgcgagt 
      ttgtgacga ttgttacaa ggaggagac gccaactat 
      caaccacgt ggccgatgg cacggaact tcgcagtcg 
      gagaatagg agaaagcgt tataaactc gatatcagt 
      cgttaagtc gattctgga caagccgaa agaactgat 
      aaaggaaac tgcaaggcc ggcgtactg gacgcccga 
      taacgaatg cagaaactg tgcagtatg tgagagacc 
      gtttggggc gcttttggg gctgcacca ctgccggga 
      ttgtggtaa gggggtgtg tgataaatg ctcgacgac 
      gcggaaacc ggtgccact gcgagggtg ggaaacacc 
      agtccgagt ttgtgataa atgttcaag ttaagatgc 
      tcaaaattt gttacaagt ctgagtaag gacacagta 
      gctttcgat aaagcattt acgtaggtt tgtactttt 
      aataaatcc gtgtgaacg tccttgtgt ggtcttttt 
      tattttata gggttatgg aaatatacg ccggttaga 
      gattatgct tgttaataa cttatagtt tattttatt 
      gtgacggag tgagtgatg taatattta cctcgctca 
      gtttttcac taaaacggt attttttat taggtttcg 
      agtcacaga actgtaccc cttagagta tttcaataa 
      cacaaatgt gtgctttta taacaagtc caattgtca 
      gtatagaat gtttttttt ggaataatt ttttttctt 
      gtacagtgg cagattttg tatcaaatg tacatacat 
      ttaggcgaa ttttttatt agaaaatta aggtgttta 
      aattaagtt tcaagcttt acattccaa attttaatg 
      tgagcaggt tactactaa aaatttgtg catttatga 
      tcaatttat ttctttatt tgttatttt catcaaagc 
      atcgacaca aaaactatt tttagtgat gacaatttc 
      attaaattc atattgtga tacaatgta aatacttag 
      ttgtatgta cataataaa attgttcat ttaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY